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The map label for this gene is leuD2 [H]

Identifier: 114799245

GI number: 114799245

Start: 1084972

End: 1085595

Strand: Direct

Name: leuD2 [H]

Synonym: HNE_1078

Alternate gene names: 114799245

Gene position: 1084972-1085595 (Clockwise)

Preceding gene: 114800141

Following gene: 114797911

Centisome position: 29.28

GC content: 63.14

Gene sequence:

>624_bases
ATGCCGAGCCCTTTTCGTCACCATCGCGGACTGGCTGCGGCGCTGCCGGTCGACAATATCGATACCGACCAGATCATTCC
CTCGCGGGAGATGAAGACGGTCAGCCGCGACGGGCTGGGCGAGGGGCTGTTTGCGGGCTGGCGCTATACAGAGCCGGGTG
GGCGCGAGCCGGTGGCGGATTTTGTGCTGAACCGGCCTGAGCAGGCGGGCACGTCAATCCTGATTTCCGGATCGAATTTT
GGCTGCGGATCATCGCGGGAACACGCCGTCTGGGCGCTGATGGATTATGGTGTGCGGGCGGTGATTGCCAAATCGTTTGG
CGAGATTTTTCATGGCAACTGTCTGCGCAATGGCATCTTGCCGGTTACCCTGCCGGAAGAGGACATTGACCGCCTGATCG
CGCAGGCGAGCGGCGGCGAGATCATGATTGATCTTGCCGCGCAGACAGTGACGGCCGGCGCGCTGCCGGGCTGGACGGGG
CATTTCGAGATTGGCGCCTATTCCAAGCGGTTGCTGCTGGAGGGGCTCGACCCGATCGGGCTGACGCTGTTGCAGGCGCC
TTCCATCAGTGCGTTTCAGGAAGCGGACGCCAGGGCGCGCCCCTGGGTTTATTCCGCTGGCTGA

Upstream 100 bases:

>100_bases
TGCGCACCCATCTGGCAAGCCCGGCTGTGGTGGCGGCGTCTGCCATTGCGGGGCGTATTTGTGCGCCATCTGACATTCTT
GTTGAAGGAGGCGTTGCCTG

Downstream 100 bases:

>100_bases
GCTTCGCTGACTTCGACAAGGCCGCGGCTGATGAGACTGTCGATCTGGTCCTGCGCGTGGCCGAGGGATTTCAGGATTTC
CACCGAATGGGCGCCGATGC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPSPFRHHRGLAAALPVDNIDTDQIIPSREMKTVSRDGLGEGLFAGWRYTEPGGREPVADFVLNRPEQAGTSILISGSNF
GCGSSREHAVWALMDYGVRAVIAKSFGEIFHGNCLRNGILPVTLPEEDIDRLIAQASGGEIMIDLAAQTVTAGALPGWTG
HFEIGAYSKRLLLEGLDPIGLTLLQAPSISAFQEADARARPWVYSAG

Sequences:

>Translated_207_residues
MPSPFRHHRGLAAALPVDNIDTDQIIPSREMKTVSRDGLGEGLFAGWRYTEPGGREPVADFVLNRPEQAGTSILISGSNF
GCGSSREHAVWALMDYGVRAVIAKSFGEIFHGNCLRNGILPVTLPEEDIDRLIAQASGGEIMIDLAAQTVTAGALPGWTG
HFEIGAYSKRLLLEGLDPIGLTLLQAPSISAFQEADARARPWVYSAG
>Mature_206_residues
PSPFRHHRGLAAALPVDNIDTDQIIPSREMKTVSRDGLGEGLFAGWRYTEPGGREPVADFVLNRPEQAGTSILISGSNFG
CGSSREHAVWALMDYGVRAVIAKSFGEIFHGNCLRNGILPVTLPEEDIDRLIAQASGGEIMIDLAAQTVTAGALPGWTGH
FEIGAYSKRLLLEGLDPIGLTLLQAPSISAFQEADARARPWVYSAG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=45.4081632653061, Blast_Score=174, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321429, Length=195, Percent_Identity=42.5641025641026, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22212; Mature: 22081

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPFRHHRGLAAALPVDNIDTDQIIPSREMKTVSRDGLGEGLFAGWRYTEPGGREPVAD
CCCCCCHHCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCEECCCCCCCHHHH
FVLNRPEQAGTSILISGSNFGCGSSREHAVWALMDYGVRAVIAKSFGEIFHGNCLRNGIL
HHHCCCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCE
PVTLPEEDIDRLIAQASGGEIMIDLAAQTVTAGALPGWTGHFEIGAYSKRLLLEGLDPIG
EEECCHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCCCEECCHHHHHHHHHCCCCCC
LTLLQAPSISAFQEADARARPWVYSAG
EEEECCCCCHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
PSPFRHHRGLAAALPVDNIDTDQIIPSREMKTVSRDGLGEGLFAGWRYTEPGGREPVAD
CCCCCHHCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCEECCCCCCCHHHH
FVLNRPEQAGTSILISGSNFGCGSSREHAVWALMDYGVRAVIAKSFGEIFHGNCLRNGIL
HHHCCCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCE
PVTLPEEDIDRLIAQASGGEIMIDLAAQTVTAGALPGWTGHFEIGAYSKRLLLEGLDPIG
EEECCHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCCCEECCHHHHHHHHHCCCCCC
LTLLQAPSISAFQEADARARPWVYSAG
EEEECCCCCHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]