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The map label for this gene is leuC [H]

Identifier: 114800141

GI number: 114800141

Start: 1083581

End: 1084972

Strand: Direct

Name: leuC [H]

Synonym: HNE_1077

Alternate gene names: 114800141

Gene position: 1083581-1084972 (Clockwise)

Preceding gene: 114799364

Following gene: 114799245

Centisome position: 29.25

GC content: 65.8

Gene sequence:

>1392_bases
GTGGCGACCTTATTTGACAAGCTGTGGGATATGCACCGGGTGGGTGATGCGGGCGACGGGGACGACCTGATCGCCATCGA
CCGGCTGTTGCTGCATGAGCGCACGGGGGGGGTGGCGCTGAAATCTCTGGCGGCGGCCGGGCACCGCGTGCGCGACCCGG
CGCGCGTATTCTCGATCATGGATCATATCGTGTCGTTCCGGCACGGACGGGGGCGCGATGAGGCGCGCAGCCCTGGCGGG
GAAGCGTTCATCGTCGAAACCCGCGAGATGGCGCAGACAGCGGGCATTCACCTGATTGATACGGATAACCCGGCGCAGGG
GATTGTGCATGTCGTGGCGCCGGAGCTGGGCATTGCGTTGCCGGGGCTGACGCTGGTGTGCCCTGACAGCCATACGTGCA
GCCTGGGGGCGCTGGGCGCGCTGGCCTGGGGCATCGGCTCCAGCGACGCCGAACATGCGATGGCGACCGGCACGCTGCGC
GCGGCAAAGCCAAGACAGATGCTGATCCGCGTGGCGGGCAGGCTTAGCCCCGGCGTTTCGGCAAAGGATCTGGCGCTTTA
TATCATTGCGCGGTTTGGCTCGGATGGCGGCAAGCGCTGCGCGATTGAATATGCGGGCGAGGCGATTTCCGATCTGAGCA
TCGAGGGGCGCCTGACGCTGTGCAATATGGCGGTGGAGTTTGCCGCGTTTACGGCGCTGATTGCGCCGGATGCCAAGACG
CTCGACTATGTCAGTGGGCGGCCGTTTTCGCCTTCAGGCGCGCAGGTGGAAACCGCCCTGGCGCATTGGCAGACGCTGCG
CACGGATGAAGGCGCGGAGTTTGACGAGGTGATTGAGGTAGACGCCGGCAAGGTGGCGCCGCAGGTGAGCTGGGGAACGA
GCCCGGAGGATAGCCTCGCGCTGACAGACGCGCTGCCCGCAGACGCGAGCGCGCGGGCGCTGGCCTATATGGGGCTGACG
GCCGGGCAATCGGTCAAAGGTCTTGCGATTGGCGGGGCGTTTATCGGCTCGTGCACCAATGGGCGGCTGAGCGATCTGAA
GGCGGCGGCGGAAATTCTCAAAGGACGCCGGGTTGCGCCGGGAGTGCGCGCGGTGTGCGTGCCGGGCTCTCAAGCGGTGA
GCCGGGAAGCAGAAGCGCTGGGCATCGACCGGATATTCAAGGATGCCGGGTTTGAATGGGGCGCGCCGGGATGTGCGATG
TGTTTCTATGCGGGCGGCGAAACCTTTCCGCCGGAGACGCGCGTGATGTCGTCCACCAACCGCAATTTTGAGGGCCGGCA
GGGGCCGGGCGTGCGCACCCATCTGGCAAGCCCGGCTGTGGTGGCGGCGTCTGCCATTGCGGGGCGTATTTGTGCGCCAT
CTGACATTCTTGTTGAAGGAGGCGTTGCCTGA

Upstream 100 bases:

>100_bases
CAACCGCCGGGTGCGGCGCAATGGGGTTTGACAAGGGACCCTTTCAGATTGCATTTGTGCGCCCATATCAAAAGATATAT
CTATTGAGTTTGAGGGATTA

Downstream 100 bases:

>100_bases
TGCCGAGCCCTTTTCGTCACCATCGCGGACTGGCTGCGGCGCTGCCGGTCGACAATATCGATACCGACCAGATCATTCCC
TCGCGGGAGATGAAGACGGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MATLFDKLWDMHRVGDAGDGDDLIAIDRLLLHERTGGVALKSLAAAGHRVRDPARVFSIMDHIVSFRHGRGRDEARSPGG
EAFIVETREMAQTAGIHLIDTDNPAQGIVHVVAPELGIALPGLTLVCPDSHTCSLGALGALAWGIGSSDAEHAMATGTLR
AAKPRQMLIRVAGRLSPGVSAKDLALYIIARFGSDGGKRCAIEYAGEAISDLSIEGRLTLCNMAVEFAAFTALIAPDAKT
LDYVSGRPFSPSGAQVETALAHWQTLRTDEGAEFDEVIEVDAGKVAPQVSWGTSPEDSLALTDALPADASARALAYMGLT
AGQSVKGLAIGGAFIGSCTNGRLSDLKAAAEILKGRRVAPGVRAVCVPGSQAVSREAEALGIDRIFKDAGFEWGAPGCAM
CFYAGGETFPPETRVMSSTNRNFEGRQGPGVRTHLASPAVVAASAIAGRICAPSDILVEGGVA

Sequences:

>Translated_463_residues
MATLFDKLWDMHRVGDAGDGDDLIAIDRLLLHERTGGVALKSLAAAGHRVRDPARVFSIMDHIVSFRHGRGRDEARSPGG
EAFIVETREMAQTAGIHLIDTDNPAQGIVHVVAPELGIALPGLTLVCPDSHTCSLGALGALAWGIGSSDAEHAMATGTLR
AAKPRQMLIRVAGRLSPGVSAKDLALYIIARFGSDGGKRCAIEYAGEAISDLSIEGRLTLCNMAVEFAAFTALIAPDAKT
LDYVSGRPFSPSGAQVETALAHWQTLRTDEGAEFDEVIEVDAGKVAPQVSWGTSPEDSLALTDALPADASARALAYMGLT
AGQSVKGLAIGGAFIGSCTNGRLSDLKAAAEILKGRRVAPGVRAVCVPGSQAVSREAEALGIDRIFKDAGFEWGAPGCAM
CFYAGGETFPPETRVMSSTNRNFEGRQGPGVRTHLASPAVVAASAIAGRICAPSDILVEGGVA
>Mature_462_residues
ATLFDKLWDMHRVGDAGDGDDLIAIDRLLLHERTGGVALKSLAAAGHRVRDPARVFSIMDHIVSFRHGRGRDEARSPGGE
AFIVETREMAQTAGIHLIDTDNPAQGIVHVVAPELGIALPGLTLVCPDSHTCSLGALGALAWGIGSSDAEHAMATGTLRA
AKPRQMLIRVAGRLSPGVSAKDLALYIIARFGSDGGKRCAIEYAGEAISDLSIEGRLTLCNMAVEFAAFTALIAPDAKTL
DYVSGRPFSPSGAQVETALAHWQTLRTDEGAEFDEVIEVDAGKVAPQVSWGTSPEDSLALTDALPADASARALAYMGLTA
GQSVKGLAIGGAFIGSCTNGRLSDLKAAAEILKGRRVAPGVRAVCVPGSQAVSREAEALGIDRIFKDAGFEWGAPGCAMC
FYAGGETFPPETRVMSSTNRNFEGRQGPGVRTHLASPAVVAASAIAGRICAPSDILVEGGVA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI41352693, Length=400, Percent_Identity=28.5, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI8659555, Length=495, Percent_Identity=26.0606060606061, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI4501867, Length=439, Percent_Identity=26.879271070615, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786259, Length=461, Percent_Identity=48.1561822125813, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1787531, Length=381, Percent_Identity=26.246719160105, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081781, Length=341, Percent_Identity=24.633431085044, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=441, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=406, Percent_Identity=29.5566502463054, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17568399, Length=490, Percent_Identity=27.9591836734694, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=321, Percent_Identity=28.0373831775701, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=451, Percent_Identity=44.1241685144124, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6320440, Length=444, Percent_Identity=28.1531531531532, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=434, Percent_Identity=26.7281105990783, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=441, Percent_Identity=27.6643990929705, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI281365315, Length=440, Percent_Identity=27.7272727272727, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17864292, Length=440, Percent_Identity=27.7272727272727, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161076999, Length=436, Percent_Identity=27.5229357798165, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28571643, Length=439, Percent_Identity=26.4236902050114, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24645686, Length=411, Percent_Identity=28.7104622871046, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137564, Length=408, Percent_Identity=27.4509803921569, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 48176; Mature: 48045

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLFDKLWDMHRVGDAGDGDDLIAIDRLLLHERTGGVALKSLAAAGHRVRDPARVFSIM
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHH
DHIVSFRHGRGRDEARSPGGEAFIVETREMAQTAGIHLIDTDNPAQGIVHVVAPELGIAL
HHHHHHHCCCCCCHHCCCCCCEEEEEHHHHHHHCCEEEEECCCCHHCEEEEECCCCCEEC
PGLTLVCPDSHTCSLGALGALAWGIGSSDAEHAMATGTLRAAKPRQMLIRVAGRLSPGVS
CCCEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
AKDLALYIIARFGSDGGKRCAIEYAGEAISDLSIEGRLTLCNMAVEFAAFTALIAPDAKT
HHHHEEEEEEEECCCCCCEEEEEECCCHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCH
LDYVSGRPFSPSGAQVETALAHWQTLRTDEGAEFDEVIEVDAGKVAPQVSWGTSPEDSLA
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCEE
LTDALPADASARALAYMGLTAGQSVKGLAIGGAFIGSCTNGRLSDLKAAAEILKGRRVAP
EECCCCCCCCHHEEEEEECCCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHHHCCCCCCC
GVRAVCVPGSQAVSREAEALGIDRIFKDAGFEWGAPGCAMCFYAGGETFPPETRVMSSTN
CCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCHHHHHCCC
RNFEGRQGPGVRTHLASPAVVAASAIAGRICAPSDILVEGGVA
CCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCEEECCCCC
>Mature Secondary Structure 
ATLFDKLWDMHRVGDAGDGDDLIAIDRLLLHERTGGVALKSLAAAGHRVRDPARVFSIM
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHH
DHIVSFRHGRGRDEARSPGGEAFIVETREMAQTAGIHLIDTDNPAQGIVHVVAPELGIAL
HHHHHHHCCCCCCHHCCCCCCEEEEEHHHHHHHCCEEEEECCCCHHCEEEEECCCCCEEC
PGLTLVCPDSHTCSLGALGALAWGIGSSDAEHAMATGTLRAAKPRQMLIRVAGRLSPGVS
CCCEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
AKDLALYIIARFGSDGGKRCAIEYAGEAISDLSIEGRLTLCNMAVEFAAFTALIAPDAKT
HHHHEEEEEEEECCCCCCEEEEEECCCHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCH
LDYVSGRPFSPSGAQVETALAHWQTLRTDEGAEFDEVIEVDAGKVAPQVSWGTSPEDSLA
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCEE
LTDALPADASARALAYMGLTAGQSVKGLAIGGAFIGSCTNGRLSDLKAAAEILKGRRVAP
EECCCCCCCCHHEEEEEECCCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHHHCCCCCCC
GVRAVCVPGSQAVSREAEALGIDRIFKDAGFEWGAPGCAMCFYAGGETFPPETRVMSSTN
CCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCHHHHHCCC
RNFEGRQGPGVRTHLASPAVVAASAIAGRICAPSDILVEGGVA
CCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA