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The map label for this gene is miaA

Identifier: 114798380

GI number: 114798380

Start: 447110

End: 448060

Strand: Reverse

Name: miaA

Synonym: HNE_0452

Alternate gene names: 114798380

Gene position: 448060-447110 (Counterclockwise)

Preceding gene: 114800403

Following gene: 114799168

Centisome position: 12.09

GC content: 62.99

Gene sequence:

>951_bases
ATGCACCCTGCGATCCTGATACATGGCCCGACGGCCAGCGGAAAATCCGCCCTTGCCATAGAACTGGCCCGCAAACTTGG
CGGCGAGGTGATCAATGCCGATTCCATGCAGGTTTACAGCGACCTGCAGGTAATTTCTGCCCGTCCGACCGAAGAAGAGA
TGGCCGGCGTGCCTCATCACCTGTTTGGCTATGTCGATGCCGGGCGGCGGTATTCGACCGGAGAATGGCTGGAATCGGCG
CGCAGCGTACTGAAACGGCTGCAGCGGCAGAACAAGCATGCGGTGATTGTCGGCGGCACCGGGCTCTACCTTCTGGCGCT
GACCCAGGGGCTTTCGGATATTCCGCCGGTGCCCGAGGACATTCGCGCCGAGGTGAAAGCCATCTCCGAGAGCGAGGGCG
CTGATGGCCTGCGTCTGCGGCTCGCGCCGCATGATCCGGAACTGGCCGAGCGGCTGGGCACGGGCGACCGGCAGAGGCTG
GCCCGTGCCTATGAGGTGTGGCTGGCGACAGGGCGGCAGCTGAGCGAGTTTCAGAATGAGCGCCAGCCACCCGTGTTGAA
AGAGGGCGAGTGGGTCGGCTTTGCCCTCACCCCGCCCCGCGCGGCGCTCTACAAGAAGATCGACCGGCGGTTTGAAGGGA
TGCTGATGCAGGGCGCGGTGGCCGAGGCGCGGGCGCTGGTTTCACGCAATCTTGATCCCGAACTTCCGGCCATGAAGGCT
CTGGGCATGCCCTCGATTGCCGCCTTCGTGCGCGGCGAGATCAGCGCCGAGGAAGCCGCCGAAAGCGCCAAGCGCGAGTC
AAGGCGTTATGCCAAAAGGCAATTTACCTGGATTGGCCGGCAATTTCCCTTCTGGCCCCGCATTCCCTCGCCGGAAGTCA
GCGACCGCATGAGGGTTATTTTTGCCCTCTATCGCGAGATTGACACAGCCGATACGGAAGATTATGCCTGA

Upstream 100 bases:

>100_bases
GCAACCAAGACCCAATCCATGTGCATTCTCAAGGCCCTCTTCGAAGCAGGCCGTCTCTAGGTGGCTTTCCTCAGGGCTCG
CAAGTCCTTAGTTGTAGGGG

Downstream 100 bases:

>100_bases
ACTCCCAAGATTGAGGAAACGCCAAGTGCGCGCATATAAAGTAGTAATTCTTAAGTGCCCGTAGCTGCCCTTCGACCAGG
CAGTTGCGGGTGCGCGCACA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MHPAILIHGPTASGKSALAIELARKLGGEVINADSMQVYSDLQVISARPTEEEMAGVPHHLFGYVDAGRRYSTGEWLESA
RSVLKRLQRQNKHAVIVGGTGLYLLALTQGLSDIPPVPEDIRAEVKAISESEGADGLRLRLAPHDPELAERLGTGDRQRL
ARAYEVWLATGRQLSEFQNERQPPVLKEGEWVGFALTPPRAALYKKIDRRFEGMLMQGAVAEARALVSRNLDPELPAMKA
LGMPSIAAFVRGEISAEEAAESAKRESRRYAKRQFTWIGRQFPFWPRIPSPEVSDRMRVIFALYREIDTADTEDYA

Sequences:

>Translated_316_residues
MHPAILIHGPTASGKSALAIELARKLGGEVINADSMQVYSDLQVISARPTEEEMAGVPHHLFGYVDAGRRYSTGEWLESA
RSVLKRLQRQNKHAVIVGGTGLYLLALTQGLSDIPPVPEDIRAEVKAISESEGADGLRLRLAPHDPELAERLGTGDRQRL
ARAYEVWLATGRQLSEFQNERQPPVLKEGEWVGFALTPPRAALYKKIDRRFEGMLMQGAVAEARALVSRNLDPELPAMKA
LGMPSIAAFVRGEISAEEAAESAKRESRRYAKRQFTWIGRQFPFWPRIPSPEVSDRMRVIFALYREIDTADTEDYA
>Mature_316_residues
MHPAILIHGPTASGKSALAIELARKLGGEVINADSMQVYSDLQVISARPTEEEMAGVPHHLFGYVDAGRRYSTGEWLESA
RSVLKRLQRQNKHAVIVGGTGLYLLALTQGLSDIPPVPEDIRAEVKAISESEGADGLRLRLAPHDPELAERLGTGDRQRL
ARAYEVWLATGRQLSEFQNERQPPVLKEGEWVGFALTPPRAALYKKIDRRFEGMLMQGAVAEARALVSRNLDPELPAMKA
LGMPSIAAFVRGEISAEEAAESAKRESRRYAKRQFTWIGRQFPFWPRIPSPEVSDRMRVIFALYREIDTADTEDYA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=328, Percent_Identity=28.0487804878049, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=35.0180505415162, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=306, Percent_Identity=24.5098039215686, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=31.4878892733564, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24649830, Length=371, Percent_Identity=26.9541778975741, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HYPNA (Q0C511)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759182.1
- ProteinModelPortal:   Q0C511
- SMR:   Q0C511
- STRING:   Q0C511
- GeneID:   4288372
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0452
- NMPDR:   fig|228405.5.peg.445
- TIGR:   HNE_0452
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q0C511
- ProtClustDB:   CLSK2531816
- BioCyc:   HNEP81032:HNE_0452-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35170; Mature: 35170

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPAILIHGPTASGKSALAIELARKLGGEVINADSMQVYSDLQVISARPTEEEMAGVPHH
CCCEEEEECCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCCHHHHHCCCHH
LFGYVDAGRRYSTGEWLESARSVLKRLQRQNKHAVIVGGTGLYLLALTQGLSDIPPVPED
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCHH
IRAEVKAISESEGADGLRLRLAPHDPELAERLGTGDRQRLARAYEVWLATGRQLSEFQNE
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHC
RQPPVLKEGEWVGFALTPPRAALYKKIDRRFEGMLMQGAVAEARALVSRNLDPELPAMKA
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LGMPSIAAFVRGEISAEEAAESAKRESRRYAKRQFTWIGRQFPFWPRIPSPEVSDRMRVI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
FALYREIDTADTEDYA
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHPAILIHGPTASGKSALAIELARKLGGEVINADSMQVYSDLQVISARPTEEEMAGVPHH
CCCEEEEECCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCCHHHHHCCCHH
LFGYVDAGRRYSTGEWLESARSVLKRLQRQNKHAVIVGGTGLYLLALTQGLSDIPPVPED
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCHH
IRAEVKAISESEGADGLRLRLAPHDPELAERLGTGDRQRLARAYEVWLATGRQLSEFQNE
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHC
RQPPVLKEGEWVGFALTPPRAALYKKIDRRFEGMLMQGAVAEARALVSRNLDPELPAMKA
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LGMPSIAAFVRGEISAEEAAESAKRESRRYAKRQFTWIGRQFPFWPRIPSPEVSDRMRVI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
FALYREIDTADTEDYA
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA