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The map label for this gene is ilvB [H]

Identifier: 114799168

GI number: 114799168

Start: 445126

End: 446862

Strand: Reverse

Name: ilvB [H]

Synonym: HNE_0451

Alternate gene names: 114799168

Gene position: 446862-445126 (Counterclockwise)

Preceding gene: 114798380

Following gene: 114799718

Centisome position: 12.06

GC content: 61.89

Gene sequence:

>1737_bases
ATGATGACCGGCGCGCAGATCGTCGTAGAGGCCCTCCGGGAGCAAGGCGTATCGGTGATGTTCGGGTATCCGGGCGGCGC
GGTGCTACCGATTTATGATGCGCTCTACGGGCAGGACGCGATCCGCCATATTCTGGTGCGCCATGAACAGGGCGCCGGGC
ACGCGGCTGAAGGCTATGCGCGCTCGACGGGGAAATGCGGCGTGGCGCTGGTCACCTCCGGCCCCGGCGCGACCAATATG
GTGACCCCGATCACCGACGCGATGATGGATTCGATCCCGATGGTCGTCCTCACCGGACAGGTGCCAACGCATCTGATCGG
GACAGACGCCTTTCAGGAGTGCGATACGACCGGCATCACGCGCACCTGCGCCAAGCACAATTACCTCGTTACGGATGTGA
ACCAGCTGGCGCGCACGATCCATGAGGCGTTCTATATCGCCACCAATGGCCGCCCCGGCCCGGTCGTGATCGACATTCCC
AAGGACATCCAGTTTGCCACCGGGCCTTATGTGGGCAAGGCCGGCATCCTGAAGCCGACCTATGTGCCCCAGACTGAGCC
GGCGCCCGAGGCAATCAAGGCTGTCGTCGACCTGATGACCAAGGCGTGGCGGCCGATCTTCTATACCGGCGGCGGCGTGA
TCAATTCCGGGCCGCGCGCGTCCAAGCTGCTGCGCGAGTTGCAGGAGATGACGGGCATTCCGGTGACCTCAACGTTGATG
GGGCTGGGCGCCTTCCCCGCCAATCATGCTGACTGGCTGGGCATGGTGGGCATGCATGGCAGCTTTGAAGCCAACAACGC
CATGCATGACTGTGACCTGATGATCTGTGTCGGCGCGCGGTTTGATGACCGGGTGACGGGCCGTGTGGACGCGTTCTCGC
CCAATTCGAAGAAGGTGCACATCGACATTGATGCCTCCTCAATCAACAAGATTGTGCGGGTGGATATTCCGGTTCTGGCC
GATTGCGCGCAGGCGCTGGAAGCCATCCTCAAGGAGCTGAAAGCCCGCAAGGCGCAGCTGCCGGATCTGGCGCCCTGGAA
AGAAGAGATCGGCGCCTGGCGCGCGCGGCGCTGCTTTGCCTACAAGGGCTCCAGTGACGTGATCAAACCGCAATATGCGG
TCGAGCGCCTCTATGCCCTGACCAAGGATCATGACGCCTACATCACGACCGAAGTGGGCCAGCACCAGATGTGGGCGGCC
CAGTTCTATCATTTCAACGAGCCCAACCGCTGGATGACTTCCGGTGGCCTGGGCACGATGGGCTATGGCCTGCCCGCCGC
CGTGGGCGTTCAGGCCGCCCACCCCGACGCGCTGGTGATCGACATTGCCGGGGAAGCCTCCATCCAGATGATGATGCAGG
AACTTTCCACGGCGGTTCAGCACAAGCTGCCGGTGAAGGTGTTCATTCTCAATAATGAATGGATGGGCATGGTGCGCCAG
TGGCAGGAATTGCTGCATGGCGAGCGCTATTCCCACTCCTATTCGGAAAGCCTGCCGGATTTCGTCAAACTGGCTGACGC
GCTGGGCGCTTATGGCATCCGCTGTGATGACCCGGCCGAGCTTGACGGGAAGATCCTGGAGATGGTGAACCATCCTGGCC
CGGTCCTGTTCGATTGCCGTGTGGAGCGCGCCGAAAACTGCCTGCCGATGATTCCGTCGGGATCAGCGCACAACCAGATG
ATTCTCGGTGAGATAACGGGTAACGAGATCGGAGAGGCTGGCCGGGGGCTGGTCTAG

Upstream 100 bases:

>100_bases
TAACCCCACGCAGAAAACATCCTTCCGGAAGCTGAAGCCCATGACCACCCAGACCAAGGCCAAGCCCGCCGCTAACACGG
CAGAGACCTCCACCCCGCGC

Downstream 100 bases:

>100_bases
GCCGCCGATCGGGGCCGGAATACGGGAAGCATGATGGACGCTGCAGGTATCTTCGACTGGCTGAAGGGCAATACCGAGAT
TATTATCGGTGTTCTCTCGG

Product: acetolactate synthase large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MMTGAQIVVEALREQGVSVMFGYPGGAVLPIYDALYGQDAIRHILVRHEQGAGHAAEGYARSTGKCGVALVTSGPGATNM
VTPITDAMMDSIPMVVLTGQVPTHLIGTDAFQECDTTGITRTCAKHNYLVTDVNQLARTIHEAFYIATNGRPGPVVIDIP
KDIQFATGPYVGKAGILKPTYVPQTEPAPEAIKAVVDLMTKAWRPIFYTGGGVINSGPRASKLLRELQEMTGIPVTSTLM
GLGAFPANHADWLGMVGMHGSFEANNAMHDCDLMICVGARFDDRVTGRVDAFSPNSKKVHIDIDASSINKIVRVDIPVLA
DCAQALEAILKELKARKAQLPDLAPWKEEIGAWRARRCFAYKGSSDVIKPQYAVERLYALTKDHDAYITTEVGQHQMWAA
QFYHFNEPNRWMTSGGLGTMGYGLPAAVGVQAAHPDALVIDIAGEASIQMMMQELSTAVQHKLPVKVFILNNEWMGMVRQ
WQELLHGERYSHSYSESLPDFVKLADALGAYGIRCDDPAELDGKILEMVNHPGPVLFDCRVERAENCLPMIPSGSAHNQM
ILGEITGNEIGEAGRGLV

Sequences:

>Translated_578_residues
MMTGAQIVVEALREQGVSVMFGYPGGAVLPIYDALYGQDAIRHILVRHEQGAGHAAEGYARSTGKCGVALVTSGPGATNM
VTPITDAMMDSIPMVVLTGQVPTHLIGTDAFQECDTTGITRTCAKHNYLVTDVNQLARTIHEAFYIATNGRPGPVVIDIP
KDIQFATGPYVGKAGILKPTYVPQTEPAPEAIKAVVDLMTKAWRPIFYTGGGVINSGPRASKLLRELQEMTGIPVTSTLM
GLGAFPANHADWLGMVGMHGSFEANNAMHDCDLMICVGARFDDRVTGRVDAFSPNSKKVHIDIDASSINKIVRVDIPVLA
DCAQALEAILKELKARKAQLPDLAPWKEEIGAWRARRCFAYKGSSDVIKPQYAVERLYALTKDHDAYITTEVGQHQMWAA
QFYHFNEPNRWMTSGGLGTMGYGLPAAVGVQAAHPDALVIDIAGEASIQMMMQELSTAVQHKLPVKVFILNNEWMGMVRQ
WQELLHGERYSHSYSESLPDFVKLADALGAYGIRCDDPAELDGKILEMVNHPGPVLFDCRVERAENCLPMIPSGSAHNQM
ILGEITGNEIGEAGRGLV
>Mature_578_residues
MMTGAQIVVEALREQGVSVMFGYPGGAVLPIYDALYGQDAIRHILVRHEQGAGHAAEGYARSTGKCGVALVTSGPGATNM
VTPITDAMMDSIPMVVLTGQVPTHLIGTDAFQECDTTGITRTCAKHNYLVTDVNQLARTIHEAFYIATNGRPGPVVIDIP
KDIQFATGPYVGKAGILKPTYVPQTEPAPEAIKAVVDLMTKAWRPIFYTGGGVINSGPRASKLLRELQEMTGIPVTSTLM
GLGAFPANHADWLGMVGMHGSFEANNAMHDCDLMICVGARFDDRVTGRVDAFSPNSKKVHIDIDASSINKIVRVDIPVLA
DCAQALEAILKELKARKAQLPDLAPWKEEIGAWRARRCFAYKGSSDVIKPQYAVERLYALTKDHDAYITTEVGQHQMWAA
QFYHFNEPNRWMTSGGLGTMGYGLPAAVGVQAAHPDALVIDIAGEASIQMMMQELSTAVQHKLPVKVFILNNEWMGMVRQ
WQELLHGERYSHSYSESLPDFVKLADALGAYGIRCDDPAELDGKILEMVNHPGPVLFDCRVERAENCLPMIPSGSAHNQM
ILGEITGNEIGEAGRGLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=26.4864864864865, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=23.2142857142857, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=49.3870402802102, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1790104, Length=572, Percent_Identity=40.9090909090909, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=32.6126126126126, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=26.7272727272727, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1788716, Length=572, Percent_Identity=25, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=26.1862917398945, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=507, Percent_Identity=25.2465483234714, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=507, Percent_Identity=25.2465483234714, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=46.3917525773196, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=25.2525252525253, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=538, Percent_Identity=22.1189591078067, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=476, Percent_Identity=25.6302521008403, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62752; Mature: 62752

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTGAQIVVEALREQGVSVMFGYPGGAVLPIYDALYGQDAIRHILVRHEQGAGHAAEGYA
CCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
RSTGKCGVALVTSGPGATNMVTPITDAMMDSIPMVVLTGQVPTHLIGTDAFQECDTTGIT
CCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHCCCCCCH
RTCAKHNYLVTDVNQLARTIHEAFYIATNGRPGPVVIDIPKDIQFATGPYVGKAGILKPT
HHHHHCCEEEECHHHHHHHHHHHEEEEECCCCCCEEEECCCCCEECCCCCCCCCCCCCCC
YVPQTEPAPEAIKAVVDLMTKAWRPIFYTGGGVINSGPRASKLLRELQEMTGIPVTSTLM
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
GLGAFPANHADWLGMVGMHGSFEANNAMHDCDLMICVGARFDDRVTGRVDAFSPNSKKVH
HHCCCCCCCCCHHHEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCEEE
IDIDASSINKIVRVDIPVLADCAQALEAILKELKARKAQLPDLAPWKEEIGAWRARRCFA
EEEEHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEE
YKGSSDVIKPQYAVERLYALTKDHDAYITTEVGQHQMWAAQFYHFNEPNRWMTSGGLGTM
ECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHEECCCCCCEEECCCCCCC
GYGLPAAVGVQAAHPDALVIDIAGEASIQMMMQELSTAVQHKLPVKVFILNNEWMGMVRQ
CCCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHH
WQELLHGERYSHSYSESLPDFVKLADALGAYGIRCDDPAELDGKILEMVNHPGPVLFDCR
HHHHHCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEE
VERAENCLPMIPSGSAHNQMILGEITGNEIGEAGRGLV
HHHHCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCC
>Mature Secondary Structure
MMTGAQIVVEALREQGVSVMFGYPGGAVLPIYDALYGQDAIRHILVRHEQGAGHAAEGYA
CCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
RSTGKCGVALVTSGPGATNMVTPITDAMMDSIPMVVLTGQVPTHLIGTDAFQECDTTGIT
CCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHCCCCCCH
RTCAKHNYLVTDVNQLARTIHEAFYIATNGRPGPVVIDIPKDIQFATGPYVGKAGILKPT
HHHHHCCEEEECHHHHHHHHHHHEEEEECCCCCCEEEECCCCCEECCCCCCCCCCCCCCC
YVPQTEPAPEAIKAVVDLMTKAWRPIFYTGGGVINSGPRASKLLRELQEMTGIPVTSTLM
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
GLGAFPANHADWLGMVGMHGSFEANNAMHDCDLMICVGARFDDRVTGRVDAFSPNSKKVH
HHCCCCCCCCCHHHEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCEEE
IDIDASSINKIVRVDIPVLADCAQALEAILKELKARKAQLPDLAPWKEEIGAWRARRCFA
EEEEHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEE
YKGSSDVIKPQYAVERLYALTKDHDAYITTEVGQHQMWAAQFYHFNEPNRWMTSGGLGTM
ECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHEECCCCCCEEECCCCCCC
GYGLPAAVGVQAAHPDALVIDIAGEASIQMMMQELSTAVQHKLPVKVFILNNEWMGMVRQ
CCCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHH
WQELLHGERYSHSYSESLPDFVKLADALGAYGIRCDDPAELDGKILEMVNHPGPVLFDCR
HHHHHCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEE
VERAENCLPMIPSGSAHNQMILGEITGNEIGEAGRGLV
HHHHCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]