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The map label for this gene is echA12 [H]

Identifier: 114798363

GI number: 114798363

Start: 2555165

End: 2555962

Strand: Reverse

Name: echA12 [H]

Synonym: HNE_2443

Alternate gene names: 114798363

Gene position: 2555962-2555165 (Counterclockwise)

Preceding gene: 114799927

Following gene: 114799270

Centisome position: 68.99

GC content: 60.53

Gene sequence:

>798_bases
ATGACCAAGGCATACGAAGCGCTCATCCTTGAGCAAAAGGACGGTGTCCTGCGGATCACCATGAACCGGCCGGATCAACT
GAACGCACTGAATACCCAGCTGGTCACCGAACTGAGGGAAGTATTTCAGGCCCTCTATTTTGACCGGTCCGTGCGCGTCG
TCATCCTCGAAGGGGCAGGCCGTGCCTTCTGTGCCGGGCTGGATCTCAAAGAATCCTCCGGCTCCCGCGGCGAGCGCAAA
GTAGAGCAGGGGCTCGACCGTCAACGCTCCATCGCTGAAATCTATGTCGCCATGCGCCGCTGCCCCCAGCCGATCATCTC
AATCGTCCAGGGCTCTGCTGCCGGTGGTGGCTTTGCCCTCGCCCTTGCTTCCGACATCCGCATCCTCAGCGAAGACGCGC
GGATGAACGCTGCCTTCATCCGCATTGGCCTCACCGCCTGCGATATGGGCGTATCCTACTTCCTCCCGCGCATGGTCGGC
ATGTCGATTGCCAGCGAATACATGCTCACAGGCCGCTTCATCAGCGCCCAGCGTGCCATGGAAATCGGTCTCGCCAACCG
CGTCGTGCCGCATGCGGAACTTCATAAGGAGGCAGACGCCTTTGCCGACGATATGCTGCACGCCACGCCGCTCGGCCTGC
GTCTCACTAAGGATGGCCTGAACCAGGCGATTGATGCCGGTGGCCTTGAAGCTGTCATGGCCATTGAGGATCGCAACCAG
ATCCTGACATCCACCGACGCCAACTTCGCTGAGGGCATCGCGGCCTTCTTCGAGAAGCGCCGCCCCAACTATTCATGA

Upstream 100 bases:

>100_bases
CTGCGTTCGGGCTGGACGGGGGCGCGCTCGACCGTTTGGAGTTGCCTCAAAGAAATAGGCATTCGTCGCCGCCAGAAATG
GCGCAAAGGGAGACAGGATC

Downstream 100 bases:

>100_bases
GCCCACCCGCAACCGACTGCCTCTGCGGCATGGCTTGACCTGCCGCGGGGCGGCTTTGAAAGTTGTGCTATAACGATAAC
GGGAGTGAGGTTCGGGCCAA

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTKAYEALILEQKDGVLRITMNRPDQLNALNTQLVTELREVFQALYFDRSVRVVILEGAGRAFCAGLDLKESSGSRGERK
VEQGLDRQRSIAEIYVAMRRCPQPIISIVQGSAAGGGFALALASDIRILSEDARMNAAFIRIGLTACDMGVSYFLPRMVG
MSIASEYMLTGRFISAQRAMEIGLANRVVPHAELHKEADAFADDMLHATPLGLRLTKDGLNQAIDAGGLEAVMAIEDRNQ
ILTSTDANFAEGIAAFFEKRRPNYS

Sequences:

>Translated_265_residues
MTKAYEALILEQKDGVLRITMNRPDQLNALNTQLVTELREVFQALYFDRSVRVVILEGAGRAFCAGLDLKESSGSRGERK
VEQGLDRQRSIAEIYVAMRRCPQPIISIVQGSAAGGGFALALASDIRILSEDARMNAAFIRIGLTACDMGVSYFLPRMVG
MSIASEYMLTGRFISAQRAMEIGLANRVVPHAELHKEADAFADDMLHATPLGLRLTKDGLNQAIDAGGLEAVMAIEDRNQ
ILTSTDANFAEGIAAFFEKRRPNYS
>Mature_264_residues
TKAYEALILEQKDGVLRITMNRPDQLNALNTQLVTELREVFQALYFDRSVRVVILEGAGRAFCAGLDLKESSGSRGERKV
EQGLDRQRSIAEIYVAMRRCPQPIISIVQGSAAGGGFALALASDIRILSEDARMNAAFIRIGLTACDMGVSYFLPRMVGM
SIASEYMLTGRFISAQRAMEIGLANRVVPHAELHKEADAFADDMLHATPLGLRLTKDGLNQAIDAGGLEAVMAIEDRNQI
LTSTDANFAEGIAAFFEKRRPNYS

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=30.5882352941176, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI194097323, Length=267, Percent_Identity=31.4606741573034, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=29.3893129770992, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=31.1787072243346, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI45643119, Length=216, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260275230, Length=216, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260274832, Length=216, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=29.2307692307692, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=29.2307692307692, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI68989263, Length=180, Percent_Identity=30, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=190, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=190, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI25453481, Length=190, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=190, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=190, Percent_Identity=25.7894736842105, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI49355787, Length=190, Percent_Identity=25.7894736842105, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI116235487, Length=185, Percent_Identity=25.4054054054054, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788597, Length=272, Percent_Identity=32.7205882352941, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=32.3076923076923, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=32.6848249027237, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=32.7067669172932, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082183, Length=273, Percent_Identity=27.8388278388278, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788682, Length=210, Percent_Identity=29.0476190476191, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=29.4736842105263, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=266, Percent_Identity=33.0827067669173, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17534483, Length=278, Percent_Identity=30.5755395683453, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=34.6456692913386, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=211, Percent_Identity=31.2796208530806, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=192, Percent_Identity=30.2083333333333, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=279, Percent_Identity=27.5985663082437, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=29.4392523364486, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=216, Percent_Identity=28.7037037037037, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=188, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=188, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=198, Percent_Identity=26.7676767676768, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=25.3968253968254, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=34.0080971659919, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=34.0080971659919, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=30.8880308880309, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=29.0697674418605, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21357171, Length=184, Percent_Identity=32.0652173913043, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=24.2307692307692, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=223, Percent_Identity=26.0089686098655, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24650670, Length=182, Percent_Identity=30.2197802197802, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921018, Length=213, Percent_Identity=27.2300469483568, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29033; Mature: 28902

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAYEALILEQKDGVLRITMNRPDQLNALNTQLVTELREVFQALYFDRSVRVVILEGAG
CCCHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
RAFCAGLDLKESSGSRGERKVEQGLDRQRSIAEIYVAMRRCPQPIISIVQGSAAGGGFAL
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEE
ALASDIRILSEDARMNAAFIRIGLTACDMGVSYFLPRMVGMSIASEYMLTGRFISAQRAM
HHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGLANRVVPHAELHKEADAFADDMLHATPLGLRLTKDGLNQAIDAGGLEAVMAIEDRNQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCEEEEEECCCCC
ILTSTDANFAEGIAAFFEKRRPNYS
EEECCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKAYEALILEQKDGVLRITMNRPDQLNALNTQLVTELREVFQALYFDRSVRVVILEGAG
CCHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
RAFCAGLDLKESSGSRGERKVEQGLDRQRSIAEIYVAMRRCPQPIISIVQGSAAGGGFAL
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEE
ALASDIRILSEDARMNAAFIRIGLTACDMGVSYFLPRMVGMSIASEYMLTGRFISAQRAM
HHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGLANRVVPHAELHKEADAFADDMLHATPLGLRLTKDGLNQAIDAGGLEAVMAIEDRNQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCEEEEEECCCCC
ILTSTDANFAEGIAAFFEKRRPNYS
EEECCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]