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The map label for this gene is rpoH [H]

Identifier: 114798150

GI number: 114798150

Start: 3587521

End: 3588408

Strand: Reverse

Name: rpoH [H]

Synonym: HNE_3450

Alternate gene names: 114798150

Gene position: 3588408-3587521 (Counterclockwise)

Preceding gene: 114797906

Following gene: 114799060

Centisome position: 96.85

GC content: 62.27

Gene sequence:

>888_bases
GTGTCCAGTGCATATTCAACGACAAGCCCGGCCGACCGGCAGTTCGTGAAGACTGCGATGAAGGCGCCGCTGCTTGATCC
GGCACATGAGGCCTCGCTGGCGCGCCGCTGGCGGGAAGCGGAAGACGAAGCCGCCTTGCACGAACTCACGACCGCTTACA
TGCGCCTCGTCATTTCCATGGCAGCGAAGTTCCGTCATTACGGGCTGCCGATGGCGGACCTTGTATCGGAAGGCAATGTC
GGCCTGATGCAGGCTGCCGCGCGGTTTGAGCCGGAGCGGGAGGTGCGTTTCTCGACCTATGCCAGCTGGTGGATACGCTC
CTCCATTCAGGACTATGTGCTGCGCAACTGGTCAATCGTCCGGACCGGGACGACCTCGGCGCAGAAATCCCTCTTCTTCA
ATCTGCGCCGCCTGCGCGCCCGTATCTCCGATTTGGGAGACGGCACGATGACCCGCGAGAACAAGCAATGGGTTTCCACC
CATCTTGGTGTACCGCTGAGGGATGTCGAGATCATGTCAGCGCGCCTTTCGGGGTCGGACCGGTCTCTCAATGCGCCCTT
GACGATGGATGGGGACGCTGAGTGGCAGGACATGATCGCTGATGAGGGGGCTCCACCAGAGGAAGCCGTGATGCGATCGC
ACGACACAGCGCGCCGTCACGAGTGGATCAGTGAGGCGATGGAGGCGCTCACCCCTCGCGAGACCTACATCATCACACGC
AGACGCCTGTCGGAGGAGCCTCAGACACTCGAGGCGCTGGGCCATGAGCTGGGCGTTTCCAAGGAACGGGTGCGCCAGAT
CGAACATCAGGCGCTGTCCAAACTGAAGCGCGCGCTTGAGCAGATTGCCGGCGACCCTGAAACGGCAGGCATCGTCCCCG
ATGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCGCCGGAAAATCAAGGGGCGCCGTGATCCAGTCGCGGCATCAGCGCGTAGAGGTTTGCAAGGAACAACAAGTATT
GCCCCAGAGGAGTTGCGCGC

Downstream 100 bases:

>100_bases
GGCCCTCAGGGCGTCTCCAGTATTTCCACATTCAGCGGATGACTGATCTCCATGCGGAGGTTCCGGACATAGCCGCGCGC
GCGGATTTTTGTCTCAGGAT

Product: putative alternative sigma factor RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSSAYSTTSPADRQFVKTAMKAPLLDPAHEASLARRWREAEDEAALHELTTAYMRLVISMAAKFRHYGLPMADLVSEGNV
GLMQAAARFEPEREVRFSTYASWWIRSSIQDYVLRNWSIVRTGTTSAQKSLFFNLRRLRARISDLGDGTMTRENKQWVST
HLGVPLRDVEIMSARLSGSDRSLNAPLTMDGDAEWQDMIADEGAPPEEAVMRSHDTARRHEWISEAMEALTPRETYIITR
RRLSEEPQTLEALGHELGVSKERVRQIEHQALSKLKRALEQIAGDPETAGIVPDA

Sequences:

>Translated_295_residues
MSSAYSTTSPADRQFVKTAMKAPLLDPAHEASLARRWREAEDEAALHELTTAYMRLVISMAAKFRHYGLPMADLVSEGNV
GLMQAAARFEPEREVRFSTYASWWIRSSIQDYVLRNWSIVRTGTTSAQKSLFFNLRRLRARISDLGDGTMTRENKQWVST
HLGVPLRDVEIMSARLSGSDRSLNAPLTMDGDAEWQDMIADEGAPPEEAVMRSHDTARRHEWISEAMEALTPRETYIITR
RRLSEEPQTLEALGHELGVSKERVRQIEHQALSKLKRALEQIAGDPETAGIVPDA
>Mature_294_residues
SSAYSTTSPADRQFVKTAMKAPLLDPAHEASLARRWREAEDEAALHELTTAYMRLVISMAAKFRHYGLPMADLVSEGNVG
LMQAAARFEPEREVRFSTYASWWIRSSIQDYVLRNWSIVRTGTTSAQKSLFFNLRRLRARISDLGDGTMTRENKQWVSTH
LGVPLRDVEIMSARLSGSDRSLNAPLTMDGDAEWQDMIADEGAPPEEAVMRSHDTARRHEWISEAMEALTPRETYIITRR
RLSEEPQTLEALGHELGVSKERVRQIEHQALSKLKRALEQIAGDPETAGIVPDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=281, Percent_Identity=35.5871886120996, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=32.0610687022901, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33307; Mature: 33176

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAYSTTSPADRQFVKTAMKAPLLDPAHEASLARRWREAEDEAALHELTTAYMRLVISM
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKFRHYGLPMADLVSEGNVGLMQAAARFEPEREVRFSTYASWWIRSSIQDYVLRNWSIV
HHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RTGTTSAQKSLFFNLRRLRARISDLGDGTMTRENKQWVSTHLGVPLRDVEIMSARLSGSD
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCC
RSLNAPLTMDGDAEWQDMIADEGAPPEEAVMRSHDTARRHEWISEAMEALTPRETYIITR
CCCCCCEEECCCCHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RRLSEEPQTLEALGHELGVSKERVRQIEHQALSKLKRALEQIAGDPETAGIVPDA
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSAYSTTSPADRQFVKTAMKAPLLDPAHEASLARRWREAEDEAALHELTTAYMRLVISM
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKFRHYGLPMADLVSEGNVGLMQAAARFEPEREVRFSTYASWWIRSSIQDYVLRNWSIV
HHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RTGTTSAQKSLFFNLRRLRARISDLGDGTMTRENKQWVSTHLGVPLRDVEIMSARLSGSD
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCC
RSLNAPLTMDGDAEWQDMIADEGAPPEEAVMRSHDTARRHEWISEAMEALTPRETYIITR
CCCCCCEEECCCCHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RRLSEEPQTLEALGHELGVSKERVRQIEHQALSKLKRALEQIAGDPETAGIVPDA
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]