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The map label for this gene is citA [H]

Identifier: 114797906

GI number: 114797906

Start: 3589530

End: 3590597

Strand: Reverse

Name: citA [H]

Synonym: HNE_3453

Alternate gene names: 114797906

Gene position: 3590597-3589530 (Counterclockwise)

Preceding gene: 114798491

Following gene: 114798150

Centisome position: 96.91

GC content: 61.99

Gene sequence:

>1068_bases
ATGGATTCCGGACTTGAGAATATTGTCGCTGCAGAGACGGTTCTGTCTGATGTGGACGGACAGAACGGGCGTCTGGTCAT
TCGCGGATGGCCGGTTGAGTTACTTTCTGCATCCATGGCGTTTGAGGATACTGCGCATCTTCTGTGGGAGGGTTTTTTTG
AAGACCTGAGAGATGTGTCGTCGCTCGGGCGACAGCTGGGCGAGGCGCGTGAAGCGGCATTTGCAATCGTGTCTGGTATT
CCTGATGGGCTGGACAGTGTGTCCTTCCTGCGCGCGGGATGGGCCCTTCTGCCGGATGATGAAAGCCTTGAGACGGCGAT
CAGGCTGGCGGGATCAGCGCCGGTTCTCACTGCCGCTGCCATAAGGCGCGGCCGGGGGGAGCCCCCAATTGCGCCGAACG
CCCAGCTGTCACAGTCGGCTGATTTTCTGCATATGTTGACCGGCAAGGTGCCGCATCAGGCCGAAGCCCGCGCCTTGGAC
GCCTATCTCGTAACCGTCTGCGATCATGGCCTCAACGCATCCACGTTTGCGGCCCGCGTCGTCGCCTCGACCAGGGCGGG
GCTTACTTCGGCCGCCATTGCAGGTATTTCGGCGCTCAAAGGTCCCCTGCATGGCGGCGCGCCAGGGCCGGTGCTCGACA
TGCTCGATGCGATTGGCGTGCCGGAAAATGCCGAGCGCTGGATTGATGAGGCGTTAGCGCGGAATGAGCGGCTGATGGGA
TTTGGCCACCGGGTTTACCGGGTGCGCGACCCCCGTGCTGATGCGCTCAAGACAGCGTTGGCGCGGCTTCCGGGCACCTC
GGGACGTATCGGTTTTGCCGAACATATCGAGCGCGCTGTGCTTGGACGGCTTGCCGAGAAATATCCCCGGCGGACGCTGG
AGACGAATGTCGAATTCTATACGGCGTTGCTGCTGGAAGCACTCGGTTTGCCACGCGAAGCATTTACTGCCGTTTTTGCC
TGCGGCCGCATCATCGGCTGGACGGCCCATGCGCGTGAGCAGATCGGCGAGGGGCGTCTGGTGCGGCCCAGATCGGTCTA
TCGCGGCCCGGTTCCTGAAGCGGCCTGA

Upstream 100 bases:

>100_bases
TCTTCGCGTCCGATCCACAACATATGTTGATTATATTGATCAAGATTGACGCTGAACAGGGCCAGGGCGAATTTGATCTC
AGGAAATCAGGGAGACACTT

Downstream 100 bases:

>100_bases
GGCTCAACCCTTCAGGTTTTTGCTGAAGAGGTCCATCTGGTTGGGGTCGGCTGGTTTCTTGCGGCGGGTCTTGGTGCGGC
CGCGGCGGAAATAGAGGTCG

Product: citrate synthase 2

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MDSGLENIVAAETVLSDVDGQNGRLVIRGWPVELLSASMAFEDTAHLLWEGFFEDLRDVSSLGRQLGEAREAAFAIVSGI
PDGLDSVSFLRAGWALLPDDESLETAIRLAGSAPVLTAAAIRRGRGEPPIAPNAQLSQSADFLHMLTGKVPHQAEARALD
AYLVTVCDHGLNASTFAARVVASTRAGLTSAAIAGISALKGPLHGGAPGPVLDMLDAIGVPENAERWIDEALARNERLMG
FGHRVYRVRDPRADALKTALARLPGTSGRIGFAEHIERAVLGRLAEKYPRRTLETNVEFYTALLLEALGLPREAFTAVFA
CGRIIGWTAHAREQIGEGRLVRPRSVYRGPVPEAA

Sequences:

>Translated_355_residues
MDSGLENIVAAETVLSDVDGQNGRLVIRGWPVELLSASMAFEDTAHLLWEGFFEDLRDVSSLGRQLGEAREAAFAIVSGI
PDGLDSVSFLRAGWALLPDDESLETAIRLAGSAPVLTAAAIRRGRGEPPIAPNAQLSQSADFLHMLTGKVPHQAEARALD
AYLVTVCDHGLNASTFAARVVASTRAGLTSAAIAGISALKGPLHGGAPGPVLDMLDAIGVPENAERWIDEALARNERLMG
FGHRVYRVRDPRADALKTALARLPGTSGRIGFAEHIERAVLGRLAEKYPRRTLETNVEFYTALLLEALGLPREAFTAVFA
CGRIIGWTAHAREQIGEGRLVRPRSVYRGPVPEAA
>Mature_355_residues
MDSGLENIVAAETVLSDVDGQNGRLVIRGWPVELLSASMAFEDTAHLLWEGFFEDLRDVSSLGRQLGEAREAAFAIVSGI
PDGLDSVSFLRAGWALLPDDESLETAIRLAGSAPVLTAAAIRRGRGEPPIAPNAQLSQSADFLHMLTGKVPHQAEARALD
AYLVTVCDHGLNASTFAARVVASTRAGLTSAAIAGISALKGPLHGGAPGPVLDMLDAIGVPENAERWIDEALARNERLMG
FGHRVYRVRDPRADALKTALARLPGTSGRIGFAEHIERAVLGRLAEKYPRRTLETNVEFYTALLLEALGLPREAFTAVFA
CGRIIGWTAHAREQIGEGRLVRPRSVYRGPVPEAA

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=377, Percent_Identity=28.6472148541114, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786527, Length=362, Percent_Identity=28.4530386740331, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17555174, Length=257, Percent_Identity=28.0155642023346, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 37992; Mature: 37992

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGLENIVAAETVLSDVDGQNGRLVIRGWPVELLSASMAFEDTAHLLWEGFFEDLRDVS
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGRQLGEAREAAFAIVSGIPDGLDSVSFLRAGWALLPDDESLETAIRLAGSAPVLTAAA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
IRRGRGEPPIAPNAQLSQSADFLHMLTGKVPHQAEARALDAYLVTVCDHGLNASTFAARV
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VASTRAGLTSAAIAGISALKGPLHGGAPGPVLDMLDAIGVPENAERWIDEALARNERLMG
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
FGHRVYRVRDPRADALKTALARLPGTSGRIGFAEHIERAVLGRLAEKYPRRTLETNVEFY
CCCCEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
TALLLEALGLPREAFTAVFACGRIIGWTAHAREQIGEGRLVRPRSVYRGPVPEAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCCCCCCC
>Mature Secondary Structure
MDSGLENIVAAETVLSDVDGQNGRLVIRGWPVELLSASMAFEDTAHLLWEGFFEDLRDVS
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGRQLGEAREAAFAIVSGIPDGLDSVSFLRAGWALLPDDESLETAIRLAGSAPVLTAAA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
IRRGRGEPPIAPNAQLSQSADFLHMLTGKVPHQAEARALDAYLVTVCDHGLNASTFAARV
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VASTRAGLTSAAIAGISALKGPLHGGAPGPVLDMLDAIGVPENAERWIDEALARNERLMG
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
FGHRVYRVRDPRADALKTALARLPGTSGRIGFAEHIERAVLGRLAEKYPRRTLETNVEFY
CCCCEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
TALLLEALGLPREAFTAVFACGRIIGWTAHAREQIGEGRLVRPRSVYRGPVPEAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]