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The map label for this gene is mdeA [H]

Identifier: 114799060

GI number: 114799060

Start: 3585322

End: 3586614

Strand: Reverse

Name: mdeA [H]

Synonym: HNE_3448

Alternate gene names: 114799060

Gene position: 3586614-3585322 (Counterclockwise)

Preceding gene: 114798150

Following gene: 114797473

Centisome position: 96.8

GC content: 62.03

Gene sequence:

>1293_bases
ATGTCAAAACCTCAGAAGTCCTATCGCAAGCGCGAGATCGCGGGCCATACCCTGAGCCCGGAATCTCTGGTGATGGGATT
TGGCTACGACCCCTTCATGTCTGAAGGCTCGATCAAGCCGCCGATTTTCATGACGTCCACCTTCGCCTTCAAGTCGGCGC
GCGAAGGGGAGGCGTTGTTTCGCCGCCTCGCCGGGAGAATGGAGGTGGGCGATCCGGAAGATGCCGACCTCATCTACACC
CGGTTCAACAATCCGAACATGGAAGTGCTGGAAGACCGGCTGACGCTTTATGATGACGGGGAGGCGGCGCTCGCTTTTTC
CTCCGGAATGTCGGCCATCACGACGGCGCTTTTTGCCTGCGCGGAACCTGGTACCGGCATCCTCCATTCCAGCCCCCTCT
ATGGCGCCTCGGAAGTCTTTATCCGGAGCTTCATGCCAGACTGGGGCGTGCGCTCGGCGGAGTTCGATGCCTGGTCGACG
GAAGACCAGATCCTCAAGGCGGCGCGCGATGCGGTCGCCCGGTTCGGGCCGATCTCGGTGATCTTTACCGAATCCCCCGC
CAACCCCACCAATGCGCTGGTCGATATTGCCGCCTGCGCCCGTGTGGCCAGTATACTGGAGCAGGAGACGGGCCGGCGCC
CCATCCTGATGTGCGACAATACCATGATGGGGCCAGTCGGCCATAAGCCGCTGAAGCATGGGGCAGACCTCGCGCTTTAC
TCGCTGACCAAATATGTCGGCGGGCATTCGGACCTGATCGCCGGCGGCATTGTCGGCAATGACGAGATCCTGACCAAGGT
CCGCCGTATGCGGAATTATCTGGGAACGATGCTGGACGCCCACTCGTGCTGGCTGCTGACACGGTCTCTGGAGACGGTGC
AGCTGCGGATGGAGCGGGCCTTCTCCAATGCGCGTATCTGCGCCAATTACCTGAAGGACCATCCCAAGGTCGCCCGCGTG
ATGTATCCCGAATTCCTGGCGGCCGGCGACCCGCAGCGCAAGGTGTTCGAGGAGCAGTGCGACGCAGCGGGGGCTACCTT
TTCCTTCGATGTGACGGGCGGGAAGGAGGCTGCGTTCCGGATGCTGGACAAGCTGCAACTGATCAAGCTGGCCGTCAGCC
TGGGCGGGACCGAGAGCCTGATGTGCCATCCCGGCTCGACCACGCATTCTGCCGTTCCGGCCGATGTGCGCGCCCGGATC
GGCTTTACCGACGGCCTCATCCGCTTTTCGGTCGGCATTGAGGCCCCTGATGACTTGGTGGTCGACCTGAAACAGGCGCT
GGAACATGTGTGA

Upstream 100 bases:

>100_bases
ATCGCCCGGCAGGTGGCGGCGGCGCAAGCCACTTCGCCTTGCCAGAGCAGTCCGGAGACCGCTAAGCCTTCCCTTACGAT
AAGTATACGGGAGGAACCCC

Downstream 100 bases:

>100_bases
GCATGGATTTCTGCTTCAAAAAGAGGCGCTTTTCCGATAGGGGAAGCGCCGGATGGTCCCGTAGCTCAGCAGGATAGAGC
GACAGATTCCTAATCTGTAG

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSKPQKSYRKREIAGHTLSPESLVMGFGYDPFMSEGSIKPPIFMTSTFAFKSAREGEALFRRLAGRMEVGDPEDADLIYT
RFNNPNMEVLEDRLTLYDDGEAALAFSSGMSAITTALFACAEPGTGILHSSPLYGASEVFIRSFMPDWGVRSAEFDAWST
EDQILKAARDAVARFGPISVIFTESPANPTNALVDIAACARVASILEQETGRRPILMCDNTMMGPVGHKPLKHGADLALY
SLTKYVGGHSDLIAGGIVGNDEILTKVRRMRNYLGTMLDAHSCWLLTRSLETVQLRMERAFSNARICANYLKDHPKVARV
MYPEFLAAGDPQRKVFEEQCDAAGATFSFDVTGGKEAAFRMLDKLQLIKLAVSLGGTESLMCHPGSTTHSAVPADVRARI
GFTDGLIRFSVGIEAPDDLVVDLKQALEHV

Sequences:

>Translated_430_residues
MSKPQKSYRKREIAGHTLSPESLVMGFGYDPFMSEGSIKPPIFMTSTFAFKSAREGEALFRRLAGRMEVGDPEDADLIYT
RFNNPNMEVLEDRLTLYDDGEAALAFSSGMSAITTALFACAEPGTGILHSSPLYGASEVFIRSFMPDWGVRSAEFDAWST
EDQILKAARDAVARFGPISVIFTESPANPTNALVDIAACARVASILEQETGRRPILMCDNTMMGPVGHKPLKHGADLALY
SLTKYVGGHSDLIAGGIVGNDEILTKVRRMRNYLGTMLDAHSCWLLTRSLETVQLRMERAFSNARICANYLKDHPKVARV
MYPEFLAAGDPQRKVFEEQCDAAGATFSFDVTGGKEAAFRMLDKLQLIKLAVSLGGTESLMCHPGSTTHSAVPADVRARI
GFTDGLIRFSVGIEAPDDLVVDLKQALEHV
>Mature_429_residues
SKPQKSYRKREIAGHTLSPESLVMGFGYDPFMSEGSIKPPIFMTSTFAFKSAREGEALFRRLAGRMEVGDPEDADLIYTR
FNNPNMEVLEDRLTLYDDGEAALAFSSGMSAITTALFACAEPGTGILHSSPLYGASEVFIRSFMPDWGVRSAEFDAWSTE
DQILKAARDAVARFGPISVIFTESPANPTNALVDIAACARVASILEQETGRRPILMCDNTMMGPVGHKPLKHGADLALYS
LTKYVGGHSDLIAGGIVGNDEILTKVRRMRNYLGTMLDAHSCWLLTRSLETVQLRMERAFSNARICANYLKDHPKVARVM
YPEFLAAGDPQRKVFEEQCDAAGATFSFDVTGGKEAAFRMLDKLQLIKLAVSLGGTESLMCHPGSTTHSAVPADVRARIG
FTDGLIRFSVGIEAPDDLVVDLKQALEHV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=404, Percent_Identity=31.9306930693069, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI299473759, Length=290, Percent_Identity=34.8275862068965, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI25453487, Length=404, Percent_Identity=28.2178217821782, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1790375, Length=396, Percent_Identity=31.8181818181818, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=27.0408163265306, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=395, Percent_Identity=33.6708860759494, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71980708, Length=403, Percent_Identity=33.0024813895782, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=395, Percent_Identity=33.9240506329114, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319307, Length=399, Percent_Identity=32.0802005012531, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=27.2727272727273, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323333, Length=439, Percent_Identity=26.879271070615, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=403, Percent_Identity=28.7841191066998, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=352, Percent_Identity=25.5681818181818, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922554, Length=351, Percent_Identity=36.1823361823362, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47006; Mature: 46875

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPQKSYRKREIAGHTLSPESLVMGFGYDPFMSEGSIKPPIFMTSTFAFKSAREGEALF
CCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHH
RRLAGRMEVGDPEDADLIYTRFNNPNMEVLEDRLTLYDDGEAALAFSSGMSAITTALFAC
HHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHEEECCCEEEEECCCHHHHHHHHHHH
AEPGTGILHSSPLYGASEVFIRSFMPDWGVRSAEFDAWSTEDQILKAARDAVARFGPISV
CCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
IFTESPANPTNALVDIAACARVASILEQETGRRPILMCDNTMMGPVGHKPLKHGADLALY
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHCCCCHHHH
SLTKYVGGHSDLIAGGIVGNDEILTKVRRMRNYLGTMLDAHSCWLLTRSLETVQLRMERA
HHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FSNARICANYLKDHPKVARVMYPEFLAAGDPQRKVFEEQCDAAGATFSFDVTGGKEAAFR
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
MLDKLQLIKLAVSLGGTESLMCHPGSTTHSAVPADVRARIGFTDGLIRFSVGIEAPDDLV
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHH
VDLKQALEHV
HHHHHHHHCC
>Mature Secondary Structure 
SKPQKSYRKREIAGHTLSPESLVMGFGYDPFMSEGSIKPPIFMTSTFAFKSAREGEALF
CCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEHHHHHCCCCHHHHH
RRLAGRMEVGDPEDADLIYTRFNNPNMEVLEDRLTLYDDGEAALAFSSGMSAITTALFAC
HHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHEEECCCEEEEECCCHHHHHHHHHHH
AEPGTGILHSSPLYGASEVFIRSFMPDWGVRSAEFDAWSTEDQILKAARDAVARFGPISV
CCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
IFTESPANPTNALVDIAACARVASILEQETGRRPILMCDNTMMGPVGHKPLKHGADLALY
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHCCCCHHHH
SLTKYVGGHSDLIAGGIVGNDEILTKVRRMRNYLGTMLDAHSCWLLTRSLETVQLRMERA
HHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FSNARICANYLKDHPKVARVMYPEFLAAGDPQRKVFEEQCDAAGATFSFDVTGGKEAAFR
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
MLDKLQLIKLAVSLGGTESLMCHPGSTTHSAVPADVRARIGFTDGLIRFSVGIEAPDDLV
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHH
VDLKQALEHV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]