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The map label for this gene is pdhB [H]

Identifier: 114798083

GI number: 114798083

Start: 2048307

End: 2049719

Strand: Reverse

Name: pdhB [H]

Synonym: HNE_1976

Alternate gene names: 114798083

Gene position: 2049719-2048307 (Counterclockwise)

Preceding gene: 114799576

Following gene: 114798189

Centisome position: 55.32

GC content: 61.36

Gene sequence:

>1413_bases
ATGTCCGTAGACATCCTAATGCCTGCGCTGTCGCCAACCATGGAAGAGGGCACGCTCTCCAAATGGCTGAAGAAAGAAGG
CGATGCCATCAAGTCCGGTGACGTGATCGCCGAAATCGAAACCGACAAAGCGACGATGGAAGTCGAAGCGGTCGATGAGG
GCGTCCTCGCAAAGATCGTCGTGCCCGAGGGCACCGAGAATGTGAAGGTCAACGCCGTTATAGCCGTCCTCGCCGAAGAT
GGTGAGGACGTGTCCAAAGAAGCGTCGTCCAAACCCAAGGAAGAGAAAGCCGAGGCGGCTTCCGAGTCTGAAGAGGTGAA
GGAGTCGAAGCAGGCCGTTCCGGAACAGGAAGATCCCAAACCCAAAGCGCCCGAACAGCCCCGCGCTGCAATTGTCAAAG
ACCCCTCGCTGCCCGAAGGCACCACCTTCACCGAAACGACCGTGCGCGACGCCCTGCGCGACGCGATGGCCGAAGAGATG
CGCAAGGATGAGCGCGTCTTCGTCATGGGCGAGGAAGTTGCGCAATATCAGGGCGCCTACAAGGTCACCCGCGAACTGCT
CCAGGAATTCGGTGATCGCCGCGTGGTGGATACACCGATCACGGAGCATGGCTTTGCTGGCCTCGGTGTTGGCGCTGCCT
TTGCGGGCCTTAAACCCATTGTCGAGTTCATGACCTTCAACTTCGCCATGCAGGCCATCGACCAGATCATCAACTCTGCC
GCCAAGACGCTCTATATGTCCGGCGGTCAGATGGGCTGTCCCATCGTGTTCCGCGGCCCCAATGGTGCGGCCAGTCGCGT
CGGCGCCCAGCACAGCCAGGACTACTCTGCCTGGTACGCCCAGATCCCCGGCCTCAAAGTCATCGCGCCCTATGACGCGG
CTGACGCCAAAGGCCTTCTCAAGGCCGCGATCCGCGACCCCAATCCGGTCGTCTTCCTTGAGCATGAACTCCTCTACGGC
CAATCCTTCCCGGTACCCGATATTGACGATCACATCGTCCCCATCGGCAAGGCGGCGGTGAAGCGTGAGGGCACGGATGT
CACGCTGGTGGCCCATTCCCGCATGGTCGGCTTTGCGCTTCAGGCGGCCGAGCGCCTCGCCGAAGAAGGCATCAGCGCCG
AAGTGATCGACCTGCGCACCCTGCGCCCGCTCGACACTGACACCGTCATAGAGAGCGTGAAGAAAACCAACCGCCTCGTC
TGCTGCGAAGAAGGCTGGCGCTTCATGGGCGTCGGCGCAGAAATCGCCGCCACCGTGGTCGCCGAAGCGTTTGACTATCT
CGACGCGCCCCCCATCCGCGTCCACCAGAAAGACGTGCCCCTGCCATACGCCGCAAACCTCGAAGCGATGAGCCTTCCGA
ATGCGGACGACATCGTCGCGGCGGCGAAGAAAGTCTGCGAGGGAGCGTTCTGA

Upstream 100 bases:

>100_bases
CTGTGGACAGATGTTCTGATCGAAGAGGCGGTGTGATGCGCGTGAGTGCGCTTCGTTTGCTGCCCGCCCTAAATTCATTC
CGTATTTCGGAGTTCAAATA

Downstream 100 bases:

>100_bases
TGGCCATCAACATCACCATGCCCGCTCTCAGCCCCACGATGGAAGAGGGCACGCTCGCCAAATGGCTCGTCAAGGAAGGC
GACACCGTCAAGTCCGGCGA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSVDILMPALSPTMEEGTLSKWLKKEGDAIKSGDVIAEIETDKATMEVEAVDEGVLAKIVVPEGTENVKVNAVIAVLAED
GEDVSKEASSKPKEEKAEAASESEEVKESKQAVPEQEDPKPKAPEQPRAAIVKDPSLPEGTTFTETTVRDALRDAMAEEM
RKDERVFVMGEEVAQYQGAYKVTRELLQEFGDRRVVDTPITEHGFAGLGVGAAFAGLKPIVEFMTFNFAMQAIDQIINSA
AKTLYMSGGQMGCPIVFRGPNGAASRVGAQHSQDYSAWYAQIPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHELLYG
QSFPVPDIDDHIVPIGKAAVKREGTDVTLVAHSRMVGFALQAAERLAEEGISAEVIDLRTLRPLDTDTVIESVKKTNRLV
CCEEGWRFMGVGAEIAATVVAEAFDYLDAPPIRVHQKDVPLPYAANLEAMSLPNADDIVAAAKKVCEGAF

Sequences:

>Translated_470_residues
MSVDILMPALSPTMEEGTLSKWLKKEGDAIKSGDVIAEIETDKATMEVEAVDEGVLAKIVVPEGTENVKVNAVIAVLAED
GEDVSKEASSKPKEEKAEAASESEEVKESKQAVPEQEDPKPKAPEQPRAAIVKDPSLPEGTTFTETTVRDALRDAMAEEM
RKDERVFVMGEEVAQYQGAYKVTRELLQEFGDRRVVDTPITEHGFAGLGVGAAFAGLKPIVEFMTFNFAMQAIDQIINSA
AKTLYMSGGQMGCPIVFRGPNGAASRVGAQHSQDYSAWYAQIPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHELLYG
QSFPVPDIDDHIVPIGKAAVKREGTDVTLVAHSRMVGFALQAAERLAEEGISAEVIDLRTLRPLDTDTVIESVKKTNRLV
CCEEGWRFMGVGAEIAATVVAEAFDYLDAPPIRVHQKDVPLPYAANLEAMSLPNADDIVAAAKKVCEGAF
>Mature_469_residues
SVDILMPALSPTMEEGTLSKWLKKEGDAIKSGDVIAEIETDKATMEVEAVDEGVLAKIVVPEGTENVKVNAVIAVLAEDG
EDVSKEASSKPKEEKAEAASESEEVKESKQAVPEQEDPKPKAPEQPRAAIVKDPSLPEGTTFTETTVRDALRDAMAEEMR
KDERVFVMGEEVAQYQGAYKVTRELLQEFGDRRVVDTPITEHGFAGLGVGAAFAGLKPIVEFMTFNFAMQAIDQIINSAA
KTLYMSGGQMGCPIVFRGPNGAASRVGAQHSQDYSAWYAQIPGLKVIAPYDAADAKGLLKAAIRDPNPVVFLEHELLYGQ
SFPVPDIDDHIVPIGKAAVKREGTDVTLVAHSRMVGFALQAAERLAEEGISAEVIDLRTLRPLDTDTVIESVKKTNRLVC
CEEGWRFMGVGAEIAATVVAEAFDYLDAPPIRVHQKDVPLPYAANLEAMSLPNADDIVAAAKKVCEGAF

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=58.641975308642, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=55.2469135802469, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI4557353, Length=334, Percent_Identity=35.6287425149701, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI34101272, Length=334, Percent_Identity=35.6287425149701, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=56.7901234567901, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=56.7901234567901, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI31711992, Length=92, Percent_Identity=47.8260869565217, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI260898739, Length=58, Percent_Identity=63.7931034482759, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI133778974, Length=350, Percent_Identity=26.5714285714286, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786946, Length=110, Percent_Identity=38.1818181818182, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=325, Percent_Identity=64.6153846153846, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17506935, Length=324, Percent_Identity=39.1975308641975, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17560088, Length=135, Percent_Identity=38.5185185185185, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319698, Length=324, Percent_Identity=62.037037037037, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324258, Length=144, Percent_Identity=45.8333333333333, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321632, Length=102, Percent_Identity=44.1176470588235, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358145, Length=328, Percent_Identity=61.890243902439, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24650940, Length=328, Percent_Identity=61.890243902439, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=33.8557993730408, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=33.8557993730408, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=64.8351648351648, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=64.8351648351648, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI20129315, Length=85, Percent_Identity=48.2352941176471, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582497, Length=78, Percent_Identity=44.8717948717949, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 50712; Mature: 50581

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDILMPALSPTMEEGTLSKWLKKEGDAIKSGDVIAEIETDKATMEVEAVDEGVLAKIV
CCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
VPEGTENVKVNAVIAVLAEDGEDVSKEASSKPKEEKAEAASESEEVKESKQAVPEQEDPK
ECCCCCCEEEEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PKAPEQPRAAIVKDPSLPEGTTFTETTVRDALRDAMAEEMRKDERVFVMGEEVAQYQGAY
CCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHH
KVTRELLQEFGDRRVVDTPITEHGFAGLGVGAAFAGLKPIVEFMTFNFAMQAIDQIINSA
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKTLYMSGGQMGCPIVFRGPNGAASRVGAQHSQDYSAWYAQIPGLKVIAPYDAADAKGLL
HHHEEECCCCCCCCEEEECCCCHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHH
KAAIRDPNPVVFLEHELLYGQSFPVPDIDDHIVPIGKAAVKREGTDVTLVAHSRMVGFAL
HHHHCCCCCEEEEEEHEECCCCCCCCCCCCCEECCCHHHHHCCCCCEEEEECCHHHHHHH
QAAERLAEEGISAEVIDLRTLRPLDTDTVIESVKKTNRLVCCEEGWRFMGVGAEIAATVV
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHH
AEAFDYLDAPPIRVHQKDVPLPYAANLEAMSLPNADDIVAAAKKVCEGAF
HHHHHHCCCCCCEEECCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVDILMPALSPTMEEGTLSKWLKKEGDAIKSGDVIAEIETDKATMEVEAVDEGVLAKIV
CCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
VPEGTENVKVNAVIAVLAEDGEDVSKEASSKPKEEKAEAASESEEVKESKQAVPEQEDPK
ECCCCCCEEEEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PKAPEQPRAAIVKDPSLPEGTTFTETTVRDALRDAMAEEMRKDERVFVMGEEVAQYQGAY
CCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHH
KVTRELLQEFGDRRVVDTPITEHGFAGLGVGAAFAGLKPIVEFMTFNFAMQAIDQIINSA
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKTLYMSGGQMGCPIVFRGPNGAASRVGAQHSQDYSAWYAQIPGLKVIAPYDAADAKGLL
HHHEEECCCCCCCCEEEECCCCHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHH
KAAIRDPNPVVFLEHELLYGQSFPVPDIDDHIVPIGKAAVKREGTDVTLVAHSRMVGFAL
HHHHCCCCCEEEEEEHEECCCCCCCCCCCCCEECCCHHHHHCCCCCEEEEECCHHHHHHH
QAAERLAEEGISAEVIDLRTLRPLDTDTVIESVKKTNRLVCCEEGWRFMGVGAEIAATVV
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHH
AEAFDYLDAPPIRVHQKDVPLPYAANLEAMSLPNADDIVAAAKKVCEGAF
HHHHHHCCCCCCEEECCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]