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The map label for this gene is pdhC [H]

Identifier: 114798189

GI number: 114798189

Start: 2046976

End: 2048307

Strand: Reverse

Name: pdhC [H]

Synonym: HNE_1975

Alternate gene names: 114798189

Gene position: 2048307-2046976 (Counterclockwise)

Preceding gene: 114798083

Following gene: 114797424

Centisome position: 55.28

GC content: 61.86

Gene sequence:

>1332_bases
ATGGCCATCAACATCACCATGCCCGCTCTCAGCCCCACGATGGAAGAGGGCACGCTCGCCAAATGGCTCGTCAAGGAAGG
CGACACCGTCAAGTCCGGCGACATCATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCCGTCGATGAAG
GCACTGTTGCAAAAATCGTCGTGGCCGAAGGCTCCGAAGGGGTAAAGGTCAACGCCGTCATCGCCGTCCTGGCCGAGGAC
GGCGAGGATGCCTCATCCGTAAAGACGCCGTCGGCCGACGCTGCGCCGAAGAAGGAAGAGAAGAAGGAAGACGCTCCGAA
GGCTGGAGAAAAGAAGCCCGACGAGAAGAAGCCCGAACCGAAAAAGGAAGAGGCCAAACCCGAGCCCGCTAAAGCGGCCG
CTCCGGCCAAGTCGGATGATGGTGCGCGCCTCAAAGCCTCCCCCCTCGCCAAGCGCATCGCGGCAAATAAAGGCATCGAC
CTCAAAGCCCTCAAAGGCTCCGGCCCCCATGGCCGCATCATCAAACGCGATGTGGAAAGCGCCAAGCCTGGCGCGCAGGC
GGCTACTGCTGGCGCCGCAGCGCCTGCTTCGCCCGATGGGCTCATCCTGCCGCAGATCCTCGACGACCGGGTCTATGCGC
CCGATACCTACGAGCTGAAACCGCTCGATGGCATGCGCAAGACCGTCGCCAGGCGCCTGACGCAGAGCTTCATGCAGGTG
CCGCATTTCCCGCTTAACATCGACATCACGCTCGACAATCTGCTGACCAGCCGAGCCTCGATCAACAACGCTGCCCGCGA
AGGCGTGAAGGTCTCAGTCAACGACCTCCTCATCAAGGCCGCCGCGCTCGCGCTGATGGATGAGCCCGACTGCAATGCAT
CGTTCACGGACAAAGGCATCGCCTATCACAAGCATGCCAACGTCTCCGTCGCCGTCGCGGTCGAAGGCGGCCTGATCACC
CCGGTGATCTTCAAGGCCGAAACCAAGGGCCTGGCTGAAATCTCCGAAGAGATGAAAGACCTCGCCGCCCGCGCGCGCGA
GCGCAAGCTGAAGCCCCAGGAATATATGGGCGGCACCTTCTCCATCTCGAACCTCGGCATGTTCGGCATCAAATCCTTCG
CCTCGATTATCAACCCGCCCGAAGGCATGATCCTTTCGGTCGGCGCCGGCGAAAAACGTGCCGTGGTCGACGAGAAGGGC
AATGTCGCCGTACGGACGATCATGAGCGTCACCCTCACCTGCGACCACCGCGTCATCGGCGGCGCAGAGGGCGCCAAATG
GCTGACGGCGTTCAAGCGCTATGTGGAAACGCCCGAAGCGATGCTGCTTTAG

Upstream 100 bases:

>100_bases
ACGTGCCCCTGCCATACGCCGCAAACCTCGAAGCGATGAGCCTTCCGAATGCGGACGACATCGTCGCGGCGGCGAAGAAA
GTCTGCGAGGGAGCGTTCTG

Downstream 100 bases:

>100_bases
CTGGTGAATAAGAGGATCTAGGACATGTCCACCCAATACGACCTCATCGTCATCGGCTCCGGTCCGGGCGGCTATGTCGC
GGCGATCCGCGCGAGCCAGC

Product: pyruvate dehydrogenase complex, E2 component, dihydrolipoyllysine-residue acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAINITMPALSPTMEEGTLAKWLVKEGDTVKSGDIIAEIETDKATMEVEAVDEGTVAKIVVAEGSEGVKVNAVIAVLAED
GEDASSVKTPSADAAPKKEEKKEDAPKAGEKKPDEKKPEPKKEEAKPEPAKAAAPAKSDDGARLKASPLAKRIAANKGID
LKALKGSGPHGRIIKRDVESAKPGAQAATAGAAAPASPDGLILPQILDDRVYAPDTYELKPLDGMRKTVARRLTQSFMQV
PHFPLNIDITLDNLLTSRASINNAAREGVKVSVNDLLIKAAALALMDEPDCNASFTDKGIAYHKHANVSVAVAVEGGLIT
PVIFKAETKGLAEISEEMKDLAARARERKLKPQEYMGGTFSISNLGMFGIKSFASIINPPEGMILSVGAGEKRAVVDEKG
NVAVRTIMSVTLTCDHRVIGGAEGAKWLTAFKRYVETPEAMLL

Sequences:

>Translated_443_residues
MAINITMPALSPTMEEGTLAKWLVKEGDTVKSGDIIAEIETDKATMEVEAVDEGTVAKIVVAEGSEGVKVNAVIAVLAED
GEDASSVKTPSADAAPKKEEKKEDAPKAGEKKPDEKKPEPKKEEAKPEPAKAAAPAKSDDGARLKASPLAKRIAANKGID
LKALKGSGPHGRIIKRDVESAKPGAQAATAGAAAPASPDGLILPQILDDRVYAPDTYELKPLDGMRKTVARRLTQSFMQV
PHFPLNIDITLDNLLTSRASINNAAREGVKVSVNDLLIKAAALALMDEPDCNASFTDKGIAYHKHANVSVAVAVEGGLIT
PVIFKAETKGLAEISEEMKDLAARARERKLKPQEYMGGTFSISNLGMFGIKSFASIINPPEGMILSVGAGEKRAVVDEKG
NVAVRTIMSVTLTCDHRVIGGAEGAKWLTAFKRYVETPEAMLL
>Mature_442_residues
AINITMPALSPTMEEGTLAKWLVKEGDTVKSGDIIAEIETDKATMEVEAVDEGTVAKIVVAEGSEGVKVNAVIAVLAEDG
EDASSVKTPSADAAPKKEEKKEDAPKAGEKKPDEKKPEPKKEEAKPEPAKAAAPAKSDDGARLKASPLAKRIAANKGIDL
KALKGSGPHGRIIKRDVESAKPGAQAATAGAAAPASPDGLILPQILDDRVYAPDTYELKPLDGMRKTVARRLTQSFMQVP
HFPLNIDITLDNLLTSRASINNAAREGVKVSVNDLLIKAAALALMDEPDCNASFTDKGIAYHKHANVSVAVAVEGGLITP
VIFKAETKGLAEISEEMKDLAARARERKLKPQEYMGGTFSISNLGMFGIKSFASIINPPEGMILSVGAGEKRAVVDEKGN
VAVRTIMSVTLTCDHRVIGGAEGAKWLTAFKRYVETPEAMLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=40.7572383073497, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI203098753, Length=462, Percent_Identity=36.3636363636364, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI203098816, Length=462, Percent_Identity=36.3636363636364, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=27.7027027027027, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=42.5, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=34.9557522123894, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786946, Length=444, Percent_Identity=28.8288288288288, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786305, Length=300, Percent_Identity=32.3333333333333, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=457, Percent_Identity=41.7943107221007, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=29.3302540415704, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17538894, Length=327, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=443, Percent_Identity=31.3769751693002, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=41.1894273127753, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320352, Length=446, Percent_Identity=29.372197309417, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321632, Length=184, Percent_Identity=36.4130434782609, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129315, Length=448, Percent_Identity=38.8392857142857, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24582497, Length=437, Percent_Identity=38.2151029748284, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=27.891156462585, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24645909, Length=173, Percent_Identity=38.150289017341, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46916; Mature: 46785

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINITMPALSPTMEEGTLAKWLVKEGDTVKSGDIIAEIETDKATMEVEAVDEGTVAKIV
CEEEEECCCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCEEEEEEECCCCCEEEEE
VAEGSEGVKVNAVIAVLAEDGEDASSVKTPSADAAPKKEEKKEDAPKAGEKKPDEKKPEP
EECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
KKEEAKPEPAKAAAPAKSDDGARLKASPLAKRIAANKGIDLKALKGSGPHGRIIKRDVES
CHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
AKPGAQAATAGAAAPASPDGLILPQILDDRVYAPDTYELKPLDGMRKTVARRLTQSFMQV
CCCCCHHHHCCCCCCCCCCCEECHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHC
PHFPLNIDITLDNLLTSRASINNAAREGVKVSVNDLLIKAAALALMDEPDCNASFTDKGI
CCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCE
AYHKHANVSVAVAVEGGLITPVIFKAETKGLAEISEEMKDLAARARERKLKPQEYMGGTF
EEEECCCEEEEEEECCCEECEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEE
SISNLGMFGIKSFASIINPPEGMILSVGAGEKRAVVDEKGNVAVRTIMSVTLTCDHRVIG
EECCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCEEEEEEEEEEEEECCEEEC
GAEGAKWLTAFKRYVETPEAMLL
CCCCHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
AINITMPALSPTMEEGTLAKWLVKEGDTVKSGDIIAEIETDKATMEVEAVDEGTVAKIV
EEEEECCCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCEEEEEEECCCCCEEEEE
VAEGSEGVKVNAVIAVLAEDGEDASSVKTPSADAAPKKEEKKEDAPKAGEKKPDEKKPEP
EECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCC
KKEEAKPEPAKAAAPAKSDDGARLKASPLAKRIAANKGIDLKALKGSGPHGRIIKRDVES
CHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
AKPGAQAATAGAAAPASPDGLILPQILDDRVYAPDTYELKPLDGMRKTVARRLTQSFMQV
CCCCCHHHHCCCCCCCCCCCEECHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHC
PHFPLNIDITLDNLLTSRASINNAAREGVKVSVNDLLIKAAALALMDEPDCNASFTDKGI
CCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCE
AYHKHANVSVAVAVEGGLITPVIFKAETKGLAEISEEMKDLAARARERKLKPQEYMGGTF
EEEECCCEEEEEEECCCEECEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEE
SISNLGMFGIKSFASIINPPEGMILSVGAGEKRAVVDEKGNVAVRTIMSVTLTCDHRVIG
EECCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCEEEEEEEEEEEEECCEEEC
GAEGAKWLTAFKRYVETPEAMLL
CCCCHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]