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The map label for this gene is glyS

Identifier: 114797868

GI number: 114797868

Start: 691934

End: 694054

Strand: Direct

Name: glyS

Synonym: HNE_0685

Alternate gene names: 114797868

Gene position: 691934-694054 (Clockwise)

Preceding gene: 114798015

Following gene: 114800443

Centisome position: 18.68

GC content: 64.55

Gene sequence:

>2121_bases
ATGGCTGACCTTCTGATCGAACTTTTCTCCGAAGAAATCCCCGCGCGCATGCAGGCGAAGGCGGAGGCGGACCTTCTGGC
GGCCCTCACGGGCAAGCTGAAAGAGGCGGGCCTCGCCTGGAAGACCGCCTTTGCCGTCTCCGGCCCGCGCCGCCTCACGG
CGGTGGTCGAAGGGCTCGACGCGCGCTCGGCGGATGTGCGCGAAGAGAAGAAAGGCCCCAAGGTCGGCGCGCCCGAGCAG
GCCATCGCCGGCTTCCTGCGCGGGGCGGGCCTCACTGACATCAGCGAGGCCTCCGTCGTCTCCGACCCCAAGAAAGGCGA
CTTCTACGTTGCCTATTCCACCGTGCCGGGCCGCGACGCGAAAGACGTGATCGCGGAAGCTGTGCCTGCCATCATCCGCG
ATTTCCACTGGCCCAAATCCATGCGCTGGGGCACCGGAGAGCTGCGTTGGGTGCGCCCGCTGCAACGCATTGTCTGCGTG
CTCGATGGCGCCGTTGTGCCGTTTGAAGTGGGCGGCATTACCAGCGGCAACGAAACCGAAGGCCACCGTGTCCATGGCCG
TGGTCCGTACACGGTCACTGGGTGGGCAGATTACAAATCCCAACTCGAAGGGCAGGGGCATGTCGTCCTCTCCCGCGATG
ATCGCCGCGCCGCCATCCTGGGCGGCATCGAAAAAGCCTGCGCCAAAGCGGGTCTGCAATGGATCGAAGACAAGGGCCTT
CTGGAAGAGGTCACCGGCCTCGCCGAATGGCCCGTGGTCGTCCTTGGACAGATGGACCCGGACTTCCTCGATCTGCCGCC
TGAAGTCATCACGCTGTCGATGCGCACCCACCAGAAATACTTCGCCGCAACGCACGCGAAGACGGGCAAGCTCGCGCCAA
ACTTCATCCTCGTCGCCAACATCGCCGCCACCGATGGCGGCGCAAAGATCGCCGAGGGTAACGCCCGCGTCCTCTCGGCC
CGCCTCTCAGACGCCCGCTTCTTCTGGGAAAAGGACAAGGCCACACCGCTCGAAGTGATGGGCGAAAAACTCAAGACCAT
CGCCTTCAAGGAAGAGCTTGGCAGCCTCGGCGACAAGGTAGAGCGCGTCGCCGCACTCGCCCGCGAACTCGCGCCTGCCG
TGGGCGCTGACCCCGGTCTTGCCGAACGCGCCGCGCGCCTCGCCAAGGCTGACCTCGTCTCCGAAATGGTCGGTGAGTTC
CCCGAACTTCAGGGCGTCATGGGTCGCTACTACGCGCTCGCCGTCGGCGAAGACGCCCGCGTCGCCGACGCCATCCGCGA
CCATTACAAACCCCAGGGCCCCAGCGACTCCGTCCCCACAGACCCGGTATCCATCGCCGTCGCGCTGGCTGACAAACTCG
ACACACTCGTCGGCTTCTGGGCGATTGATGAGAAGCCAACCGGCTCGAAAGACCCGTTCGCGTTGCGCCGCGCGGCGCTA
GGCGTGGTCCGCATCAATTTGGAGAATGGTGTGAGACTGAAATTGTCTAACGCTATTCTTAAAGCTCCGCTTGCAGTGAG
GCCTGTAGTGAAGGGCGGGCAGTCAGATAGCGTGCGTTCGGCCGCCTCTAAAGCAATTGATGCTCTGATTGATCCGACTC
TCCTCTCCTTCTTCGCAGACCGCCTCAAACAAGTCCTCCGCGACCAGGGAAAACGCCACGACCTGATCGATGCCGTCTTC
GCCCTCGGCGAGGATGACCTCGTCCTCATCGTCAAGCGCGTCGAGGCCCTCGCCGCCTTCCTCGCCACCGAGGACGGGGC
AACGCTGCTGGCGGGCTATCGCCGCGCCGCCAACATCCTCAAGGCCGAAGAGAAGAAAGGCGCGCTGCCCGATGGCCTCA
GCGTCGATCCCGCCCTCATCGCCAAAGGCCCCGCCGCCGAGCAGGCCCTCTGGGCGTCCCTGAATGAGACGACCGCCGCC
CTTGAAGCGCCCCTGAAGACCGAAGACTTCGCCGGCGCCATGACGGCCCTTGCCGGCCTGCGCGCCCCGGTCGATGCCTT
CTTCGAGGATGTGCTGGTCAATGATTCGGATGCCAAAGTGCGCGAAAACCGGCTCGCCCTGCTGATCGCGGTGCGCGGCG
CCCTTCACAAGGTCGCCGATTTCTCGAGAATTGAGGGCTAA

Upstream 100 bases:

>100_bases
GTCTGACCCCCGCTGCGCAATCCATAGACCCGCCCGCTTGACCCCGGCGCCCGTAGCTGTAAGCCGTGCCCACCCCTAAT
TCCGGCGACTGACAGGGCCT

Downstream 100 bases:

>100_bases
AGACGGCGCGGCAACCTTGTCACCTTGCGTCAGGGCGCCCCATGACGTTTGCATGACTGGCAATTGCCTCGCGGTTGCAG
CATAGGACTTGCAAGCTGGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MADLLIELFSEEIPARMQAKAEADLLAALTGKLKEAGLAWKTAFAVSGPRRLTAVVEGLDARSADVREEKKGPKVGAPEQ
AIAGFLRGAGLTDISEASVVSDPKKGDFYVAYSTVPGRDAKDVIAEAVPAIIRDFHWPKSMRWGTGELRWVRPLQRIVCV
LDGAVVPFEVGGITSGNETEGHRVHGRGPYTVTGWADYKSQLEGQGHVVLSRDDRRAAILGGIEKACAKAGLQWIEDKGL
LEEVTGLAEWPVVVLGQMDPDFLDLPPEVITLSMRTHQKYFAATHAKTGKLAPNFILVANIAATDGGAKIAEGNARVLSA
RLSDARFFWEKDKATPLEVMGEKLKTIAFKEELGSLGDKVERVAALARELAPAVGADPGLAERAARLAKADLVSEMVGEF
PELQGVMGRYYALAVGEDARVADAIRDHYKPQGPSDSVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKDPFALRRAAL
GVVRINLENGVRLKLSNAILKAPLAVRPVVKGGQSDSVRSAASKAIDALIDPTLLSFFADRLKQVLRDQGKRHDLIDAVF
ALGEDDLVLIVKRVEALAAFLATEDGATLLAGYRRAANILKAEEKKGALPDGLSVDPALIAKGPAAEQALWASLNETTAA
LEAPLKTEDFAGAMTALAGLRAPVDAFFEDVLVNDSDAKVRENRLALLIAVRGALHKVADFSRIEG

Sequences:

>Translated_706_residues
MADLLIELFSEEIPARMQAKAEADLLAALTGKLKEAGLAWKTAFAVSGPRRLTAVVEGLDARSADVREEKKGPKVGAPEQ
AIAGFLRGAGLTDISEASVVSDPKKGDFYVAYSTVPGRDAKDVIAEAVPAIIRDFHWPKSMRWGTGELRWVRPLQRIVCV
LDGAVVPFEVGGITSGNETEGHRVHGRGPYTVTGWADYKSQLEGQGHVVLSRDDRRAAILGGIEKACAKAGLQWIEDKGL
LEEVTGLAEWPVVVLGQMDPDFLDLPPEVITLSMRTHQKYFAATHAKTGKLAPNFILVANIAATDGGAKIAEGNARVLSA
RLSDARFFWEKDKATPLEVMGEKLKTIAFKEELGSLGDKVERVAALARELAPAVGADPGLAERAARLAKADLVSEMVGEF
PELQGVMGRYYALAVGEDARVADAIRDHYKPQGPSDSVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKDPFALRRAAL
GVVRINLENGVRLKLSNAILKAPLAVRPVVKGGQSDSVRSAASKAIDALIDPTLLSFFADRLKQVLRDQGKRHDLIDAVF
ALGEDDLVLIVKRVEALAAFLATEDGATLLAGYRRAANILKAEEKKGALPDGLSVDPALIAKGPAAEQALWASLNETTAA
LEAPLKTEDFAGAMTALAGLRAPVDAFFEDVLVNDSDAKVRENRLALLIAVRGALHKVADFSRIEG
>Mature_705_residues
ADLLIELFSEEIPARMQAKAEADLLAALTGKLKEAGLAWKTAFAVSGPRRLTAVVEGLDARSADVREEKKGPKVGAPEQA
IAGFLRGAGLTDISEASVVSDPKKGDFYVAYSTVPGRDAKDVIAEAVPAIIRDFHWPKSMRWGTGELRWVRPLQRIVCVL
DGAVVPFEVGGITSGNETEGHRVHGRGPYTVTGWADYKSQLEGQGHVVLSRDDRRAAILGGIEKACAKAGLQWIEDKGLL
EEVTGLAEWPVVVLGQMDPDFLDLPPEVITLSMRTHQKYFAATHAKTGKLAPNFILVANIAATDGGAKIAEGNARVLSAR
LSDARFFWEKDKATPLEVMGEKLKTIAFKEELGSLGDKVERVAALARELAPAVGADPGLAERAARLAKADLVSEMVGEFP
ELQGVMGRYYALAVGEDARVADAIRDHYKPQGPSDSVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKDPFALRRAALG
VVRINLENGVRLKLSNAILKAPLAVRPVVKGGQSDSVRSAASKAIDALIDPTLLSFFADRLKQVLRDQGKRHDLIDAVFA
LGEDDLVLIVKRVEALAAFLATEDGATLLAGYRRAANILKAEEKKGALPDGLSVDPALIAKGPAAEQALWASLNETTAAL
EAPLKTEDFAGAMTALAGLRAPVDAFFEDVLVNDSDAKVRENRLALLIAVRGALHKVADFSRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=710, Percent_Identity=34.9295774647887, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_HYPNA (Q0C4C9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759414.1
- ProteinModelPortal:   Q0C4C9
- STRING:   Q0C4C9
- GeneID:   4288658
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0685
- NMPDR:   fig|228405.5.peg.672
- TIGR:   HNE_0685
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q0C4C9
- ProtClustDB:   PRK01233
- BioCyc:   HNEP81032:HNE_0685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75467; Mature: 75336

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLLIELFSEEIPARMQAKAEADLLAALTGKLKEAGLAWKTAFAVSGPRRLTAVVEGLD
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCHHHHHHHHCCC
ARSADVREEKKGPKVGAPEQAIAGFLRGAGLTDISEASVVSDPKKGDFYVAYSTVPGRDA
CCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCEEEEEECCCCCCH
KDVIAEAVPAIIRDFHWPKSMRWGTGELRWVRPLQRIVCVLDGAVVPFEVGGITSGNETE
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
GHRVHGRGPYTVTGWADYKSQLEGQGHVVLSRDDRRAAILGGIEKACAKAGLQWIEDKGL
CCEEECCCCEEEEEHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCH
LEEVTGLAEWPVVVLGQMDPDFLDLPPEVITLSMRTHQKYFAATHAKTGKLAPNFILVAN
HHHHHCCCCCCEEEEECCCCCHHCCCHHHEEEEHHHHHHHHHHHCCCCCCCCCCEEEEEE
IAATDGGAKIAEGNARVLSARLSDARFFWEKDKATPLEVMGEKLKTIAFKEELGSLGDKV
EEECCCCCEEECCCHHEEEHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVAALARELAPAVGADPGLAERAARLAKADLVSEMVGEFPELQGVMGRYYALAVGEDAR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEECCCHH
VADAIRDHYKPQGPSDSVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKDPFALRRAAL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHE
GVVRINLENGVRLKLSNAILKAPLAVRPVVKGGQSDSVRSAASKAIDALIDPTLLSFFAD
EEEEEEECCCEEEEECCHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLKQVLRDQGKRHDLIDAVFALGEDDLVLIVKRVEALAAFLATEDGATLLAGYRRAANIL
HHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KAEEKKGALPDGLSVDPALIAKGPAAEQALWASLNETTAALEAPLKTEDFAGAMTALAGL
HHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
RAPVDAFFEDVLVNDSDAKVRENRLALLIAVRGALHKVADFSRIEG
CCCHHHHHHHHHCCCCCCHHHHCCEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADLLIELFSEEIPARMQAKAEADLLAALTGKLKEAGLAWKTAFAVSGPRRLTAVVEGLD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCHHHHHHHHCCC
ARSADVREEKKGPKVGAPEQAIAGFLRGAGLTDISEASVVSDPKKGDFYVAYSTVPGRDA
CCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCEEEEEECCCCCCH
KDVIAEAVPAIIRDFHWPKSMRWGTGELRWVRPLQRIVCVLDGAVVPFEVGGITSGNETE
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
GHRVHGRGPYTVTGWADYKSQLEGQGHVVLSRDDRRAAILGGIEKACAKAGLQWIEDKGL
CCEEECCCCEEEEEHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCH
LEEVTGLAEWPVVVLGQMDPDFLDLPPEVITLSMRTHQKYFAATHAKTGKLAPNFILVAN
HHHHHCCCCCCEEEEECCCCCHHCCCHHHEEEEHHHHHHHHHHHCCCCCCCCCCEEEEEE
IAATDGGAKIAEGNARVLSARLSDARFFWEKDKATPLEVMGEKLKTIAFKEELGSLGDKV
EEECCCCCEEECCCHHEEEHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVAALARELAPAVGADPGLAERAARLAKADLVSEMVGEFPELQGVMGRYYALAVGEDAR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEECCCHH
VADAIRDHYKPQGPSDSVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKDPFALRRAAL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHE
GVVRINLENGVRLKLSNAILKAPLAVRPVVKGGQSDSVRSAASKAIDALIDPTLLSFFAD
EEEEEEECCCEEEEECCHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLKQVLRDQGKRHDLIDAVFALGEDDLVLIVKRVEALAAFLATEDGATLLAGYRRAANIL
HHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KAEEKKGALPDGLSVDPALIAKGPAAEQALWASLNETTAALEAPLKTEDFAGAMTALAGL
HHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
RAPVDAFFEDVLVNDSDAKVRENRLALLIAVRGALHKVADFSRIEG
CCCHHHHHHHHHCCCCCCHHHHCCEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA