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The map label for this gene is ppdK [H]

Identifier: 114800443

GI number: 114800443

Start: 694206

End: 696923

Strand: Direct

Name: ppdK [H]

Synonym: HNE_0686

Alternate gene names: 114800443

Gene position: 694206-696923 (Clockwise)

Preceding gene: 114797868

Following gene: 114797673

Centisome position: 18.74

GC content: 63.54

Gene sequence:

>2718_bases
ATGGGCAACGCCGCAGTAAAAAACGCTGACGAAACATGGGTCTACGCTTTCGGAGGCGGAACCTCGGATGGCGATGCCTC
GATGCGCAACCTCCTGGGGGGCAAAGGCGCCAACCTTGCCGAAATGGCAAAGCTCGGCCTGCCTGTGCCGCCCGGCTTCA
CGATTTCGACCGCTGTCTGCGTCGCTTACTATGATACCGGCCGGGTTTATCCCGCCACGCTCGACGCGCAGGTCTCTGAC
GCGCTGGCCGCGCTGGAAGGCAAAACCGGCAAGGGTTTCGGCGATCCGGAAAACCCGCTCCTCGTCTCGGTCCGCTCGGG
CGCCCGCGCCTCGATGCCCGGTATGATGGACACGGTTCTGAACCTCGGCCTCAACGAAGCTGTCGCCGCCGGCCTCGCCG
GCAAGGCAGGCGACCGTTTCGCCTATGACAGCTACCGCCGTTTCATCCAGATGTATTCCAACGTCGTCCTCGGCATGGGC
CATGACGAGTTCGAACACATCCTCGACGACTATAAGGAGCGCGAAGGTCTCGACCTCGATACCGAGTTGACCGCCGACAA
CTGGAAACAGGTCATCGTGCGCTACAAGGAAGCCGTCCAGAAGGAACTGGGCCGCCCGTTCCCCGAAGATCCGCGCGAAC
AGCTCTGGGGCGCCATCTCGGCAGTGTTCGACTCCTGGATGTCCGACCGCGCGATCATCTATCGCAAGCTCAACGACATT
CCCGCCAACTGGGGCACGGCCGTCAACGTCCAGTCCATGGTGTTCGGCAATCTGGGCGACACGTCTGCCACCGGCGTTGC
CTTCACGCGTGACCCGTCAAACGGCTCGCCGATCTTCTATGGCGAATACCTGATCAACGCCCAGGGCGAAGATGTCGTCG
CCGGCATCCGCACGCCTGCGCCCATCTCGCGCGAGCGCGCCAATACGCTCGGCTCGTCGGACGCCCCGCTCGAAGAGGCC
ATGCCGCCGGTCTACAAACAGCTGGTCGACGTCGCCAACACGCTGGAGCGTCACTACAAGGATATGCAGGACATCGAGTT
CACCGTCGAAGACGGCACGCTCTACATGCTGCAGACCCGCAACGGCAAACGCACCGCCGCCGCCGCTGTGAAGATTGCGG
TCGATATGGTCCGCGAAGGTCTGATCACGGAAGAAGAGGCGCTGCTGCGCATCGACCCCACCGCGCTCGACCAGCTTCTC
CACCCGCGCATCCCCAGCGATGACGAGAACAAGAAAGCTGGAAACGCCGCCTATGACGTCGCCTTCAAAGGCCTTCCCGC
CAGCCCCGGCGCGGCTGTCGGCCGCGTTGTGTTCGATTCCGAAGAAGCCTTCGCCCTTGCCGCAAAAGGGGAGGACGTGA
TCCTCGTCCGTGTCGAGACGAGCCCGGATGACATCAAGGGCATGCATGCGGCCCGCGCCATCGTCACCGCCCGCGGCGGC
ATGACCAGCCACGCCGCCGTTGTCGCGCGCGGCATGGGCCGTCCCTGCGTCTGCGGCGCGTCGGACCTGCGCATCAACAC
CGCCAAAGGCGAATTCACCGCCCGTGGCCGCACCTTCAAGAAGGGCGACATGATCACCGTTGACGGCGTCAATGGCCGCG
TCATTGCCGGGGCCGTTTCGCTGATCAATCCCGACCTCTCCGGCGATTTCGGTGAGTTCATGGGCTGGGCCGACAAGCGC
CGCACCCTGAAGGTTCGCGCCAATGCCGAAACCCCGCTCGACACGCGCACCGCCGTCGAGTTCGGCGCTGAAGGCGTCGG
CCTCTGCCGCACCGAGCATATGTTCTTCGACGAGGAACGCATCCCCGCCGTGCGCACGATGATCCTCTCCAATGACGAAA
CCGGTCGCCGCGAGGCGCTCGCCAAGCTGCTCCCGATGCAGCGTTCCGACTTTGAAGAAATCTTCCGCATCCTCGGCGAT
CGCCCGGCGACCATCCGCCTGCTCGATCCGCCGCTGCACGAATTCCTGCCCCACACCGATGAAGACATCGCCGGCGTCGC
CAAGGCTTCGGGCCTGAGCGCCGAGGAACTGCGCCGCCGCGCCGCTGACCTGCACGAATCCAACCCCATGCTCGGCCACC
GGGGCTGCCGCCTCGGCATCACCTATCCTGAAATCTACGAGATGCAGGCCCGCGCCATCTTTGAAGCGGCGGTCAACATC
TTCAAGGAAGGCAAGCTGAAGCCCGTTCCCGAGGTGATGATCCCGCTGGTCGCCACCCACCGCGAACTCGACATCCTGAA
AAAGCTCGTCGACGAAACCGCCCAGAAAGTGTTCGCCGAAACCGGTGTCACGCTCGACTATATGGTCGGCACGATGATCG
AGCTGCCCCGCGCGGCTCTCGCGGCAGGGGAAATTGCCCAGGCCGCCGAATTCTTCTCCTTCGGCACGAACGATCTCACC
CAGACAACCCTCGGAATTTCCCGCGATGACGCCGGCAAATTCCTCCCGATCTATTCGGAAAAGGGCATCTATGAGAAAGA
CCCGTTCGTAACACTTGACCAGGAAGGTGTGGGCGAACTCGTTAAGATTGCGGCCGAGCGCGGGCGCAAAACCCGCCCCG
ACATCAAGCTCGGAATCTGTGGTGAACACGGGGGAGACCCGGCTTCGGTGGAATTCTGTCACCGCGCCGGTCTCGATTAC
GTTTCCTGCTCGCCCTACCGGGTGCCGATTGCCCGTCTCGCAGCGGCACAAGCCGCCATCCGCGGAGCGAAGAAGTAG

Upstream 100 bases:

>100_bases
CATGACTGGCAATTGCCTCGCGGTTGCAGCATAGGACTTGCAAGCTGGCCGCAAGGTCAGGTGGCCGGAAGATCAAAGTT
GAACACGGGGAACGCTTCGT

Downstream 100 bases:

>100_bases
CGGATTTCGGGCAGTTGAGGCGCAAAACCCGCCGCCCTTGAACGTTGCATTTGTGCAACAACTTGACAGGAAGATTCTAA
CCAACGGGACCGCGCGCGGT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MGNAAVKNADETWVYAFGGGTSDGDASMRNLLGGKGANLAEMAKLGLPVPPGFTISTAVCVAYYDTGRVYPATLDAQVSD
ALAALEGKTGKGFGDPENPLLVSVRSGARASMPGMMDTVLNLGLNEAVAAGLAGKAGDRFAYDSYRRFIQMYSNVVLGMG
HDEFEHILDDYKEREGLDLDTELTADNWKQVIVRYKEAVQKELGRPFPEDPREQLWGAISAVFDSWMSDRAIIYRKLNDI
PANWGTAVNVQSMVFGNLGDTSATGVAFTRDPSNGSPIFYGEYLINAQGEDVVAGIRTPAPISRERANTLGSSDAPLEEA
MPPVYKQLVDVANTLERHYKDMQDIEFTVEDGTLYMLQTRNGKRTAAAAVKIAVDMVREGLITEEEALLRIDPTALDQLL
HPRIPSDDENKKAGNAAYDVAFKGLPASPGAAVGRVVFDSEEAFALAAKGEDVILVRVETSPDDIKGMHAARAIVTARGG
MTSHAAVVARGMGRPCVCGASDLRINTAKGEFTARGRTFKKGDMITVDGVNGRVIAGAVSLINPDLSGDFGEFMGWADKR
RTLKVRANAETPLDTRTAVEFGAEGVGLCRTEHMFFDEERIPAVRTMILSNDETGRREALAKLLPMQRSDFEEIFRILGD
RPATIRLLDPPLHEFLPHTDEDIAGVAKASGLSAEELRRRAADLHESNPMLGHRGCRLGITYPEIYEMQARAIFEAAVNI
FKEGKLKPVPEVMIPLVATHRELDILKKLVDETAQKVFAETGVTLDYMVGTMIELPRAALAAGEIAQAAEFFSFGTNDLT
QTTLGISRDDAGKFLPIYSEKGIYEKDPFVTLDQEGVGELVKIAAERGRKTRPDIKLGICGEHGGDPASVEFCHRAGLDY
VSCSPYRVPIARLAAAQAAIRGAKK

Sequences:

>Translated_905_residues
MGNAAVKNADETWVYAFGGGTSDGDASMRNLLGGKGANLAEMAKLGLPVPPGFTISTAVCVAYYDTGRVYPATLDAQVSD
ALAALEGKTGKGFGDPENPLLVSVRSGARASMPGMMDTVLNLGLNEAVAAGLAGKAGDRFAYDSYRRFIQMYSNVVLGMG
HDEFEHILDDYKEREGLDLDTELTADNWKQVIVRYKEAVQKELGRPFPEDPREQLWGAISAVFDSWMSDRAIIYRKLNDI
PANWGTAVNVQSMVFGNLGDTSATGVAFTRDPSNGSPIFYGEYLINAQGEDVVAGIRTPAPISRERANTLGSSDAPLEEA
MPPVYKQLVDVANTLERHYKDMQDIEFTVEDGTLYMLQTRNGKRTAAAAVKIAVDMVREGLITEEEALLRIDPTALDQLL
HPRIPSDDENKKAGNAAYDVAFKGLPASPGAAVGRVVFDSEEAFALAAKGEDVILVRVETSPDDIKGMHAARAIVTARGG
MTSHAAVVARGMGRPCVCGASDLRINTAKGEFTARGRTFKKGDMITVDGVNGRVIAGAVSLINPDLSGDFGEFMGWADKR
RTLKVRANAETPLDTRTAVEFGAEGVGLCRTEHMFFDEERIPAVRTMILSNDETGRREALAKLLPMQRSDFEEIFRILGD
RPATIRLLDPPLHEFLPHTDEDIAGVAKASGLSAEELRRRAADLHESNPMLGHRGCRLGITYPEIYEMQARAIFEAAVNI
FKEGKLKPVPEVMIPLVATHRELDILKKLVDETAQKVFAETGVTLDYMVGTMIELPRAALAAGEIAQAAEFFSFGTNDLT
QTTLGISRDDAGKFLPIYSEKGIYEKDPFVTLDQEGVGELVKIAAERGRKTRPDIKLGICGEHGGDPASVEFCHRAGLDY
VSCSPYRVPIARLAAAQAAIRGAKK
>Mature_904_residues
GNAAVKNADETWVYAFGGGTSDGDASMRNLLGGKGANLAEMAKLGLPVPPGFTISTAVCVAYYDTGRVYPATLDAQVSDA
LAALEGKTGKGFGDPENPLLVSVRSGARASMPGMMDTVLNLGLNEAVAAGLAGKAGDRFAYDSYRRFIQMYSNVVLGMGH
DEFEHILDDYKEREGLDLDTELTADNWKQVIVRYKEAVQKELGRPFPEDPREQLWGAISAVFDSWMSDRAIIYRKLNDIP
ANWGTAVNVQSMVFGNLGDTSATGVAFTRDPSNGSPIFYGEYLINAQGEDVVAGIRTPAPISRERANTLGSSDAPLEEAM
PPVYKQLVDVANTLERHYKDMQDIEFTVEDGTLYMLQTRNGKRTAAAAVKIAVDMVREGLITEEEALLRIDPTALDQLLH
PRIPSDDENKKAGNAAYDVAFKGLPASPGAAVGRVVFDSEEAFALAAKGEDVILVRVETSPDDIKGMHAARAIVTARGGM
TSHAAVVARGMGRPCVCGASDLRINTAKGEFTARGRTFKKGDMITVDGVNGRVIAGAVSLINPDLSGDFGEFMGWADKRR
TLKVRANAETPLDTRTAVEFGAEGVGLCRTEHMFFDEERIPAVRTMILSNDETGRREALAKLLPMQRSDFEEIFRILGDR
PATIRLLDPPLHEFLPHTDEDIAGVAKASGLSAEELRRRAADLHESNPMLGHRGCRLGITYPEIYEMQARAIFEAAVNIF
KEGKLKPVPEVMIPLVATHRELDILKKLVDETAQKVFAETGVTLDYMVGTMIELPRAALAAGEIAQAAEFFSFGTNDLTQ
TTLGISRDDAGKFLPIYSEKGIYEKDPFVTLDQEGVGELVKIAAERGRKTRPDIKLGICGEHGGDPASVEFCHRAGLDYV
SCSPYRVPIARLAAAQAAIRGAKK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=462, Percent_Identity=25.5411255411255, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789193, Length=441, Percent_Identity=24.4897959183673, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788726, Length=446, Percent_Identity=24.8878923766816, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI48994992, Length=347, Percent_Identity=24.4956772334294, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 98048; Mature: 97917

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNAAVKNADETWVYAFGGGTSDGDASMRNLLGGKGANLAEMAKLGLPVPPGFTISTAVC
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHEEE
VAYYDTGRVYPATLDAQVSDALAALEGKTGKGFGDPENPLLVSVRSGARASMPGMMDTVL
EEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHH
NLGLNEAVAAGLAGKAGDRFAYDSYRRFIQMYSNVVLGMGHDEFEHILDDYKEREGLDLD
HCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCC
TELTADNWKQVIVRYKEAVQKELGRPFPEDPREQLWGAISAVFDSWMSDRAIIYRKLNDI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEHHHHCC
PANWGTAVNVQSMVFGNLGDTSATGVAFTRDPSNGSPIFYGEYLINAQGEDVVAGIRTPA
CCCCCCEEEEHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCC
PISRERANTLGSSDAPLEEAMPPVYKQLVDVANTLERHYKDMQDIEFTVEDGTLYMLQTR
CCCHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHCEEEEECCEEEEEEEC
NGKRTAAAAVKIAVDMVREGLITEEEALLRIDPTALDQLLHPRIPSDDENKKAGNAAYDV
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCEEEE
AFKGLPASPGAAVGRVVFDSEEAFALAAKGEDVILVRVETSPDDIKGMHAARAIVTARGG
EECCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHEECCC
MTSHAAVVARGMGRPCVCGASDLRINTAKGEFTARGRTFKKGDMITVDGVNGRVIAGAVS
CCHHHHHHHHCCCCCEEECCCCCEEEECCCCEEECCCEECCCCEEEEECCCCEEEEEEHH
LINPDLSGDFGEFMGWADKRRTLKVRANAETPLDTRTAVEFGAEGVGLCRTEHMFFDEER
HHCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCC
IPAVRTMILSNDETGRREALAKLLPMQRSDFEEIFRILGDRPATIRLLDPPLHEFLPHTD
CCHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHCCCCC
EDIAGVAKASGLSAEELRRRAADLHESNPMLGHRGCRLGITYPEIYEMQARAIFEAAVNI
CHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
FKEGKLKPVPEVMIPLVATHRELDILKKLVDETAQKVFAETGVTLDYMVGTMIELPRAAL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AAGEIAQAAEFFSFGTNDLTQTTLGISRDDAGKFLPIYSEKGIYEKDPFVTLDQEGVGEL
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHHHHH
VKIAAERGRKTRPDIKLGICGEHGGDPASVEFCHRAGLDYVSCSPYRVPIARLAAAQAAI
HHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RGAKK
HCCCC
>Mature Secondary Structure 
GNAAVKNADETWVYAFGGGTSDGDASMRNLLGGKGANLAEMAKLGLPVPPGFTISTAVC
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHEEE
VAYYDTGRVYPATLDAQVSDALAALEGKTGKGFGDPENPLLVSVRSGARASMPGMMDTVL
EEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHH
NLGLNEAVAAGLAGKAGDRFAYDSYRRFIQMYSNVVLGMGHDEFEHILDDYKEREGLDLD
HCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCC
TELTADNWKQVIVRYKEAVQKELGRPFPEDPREQLWGAISAVFDSWMSDRAIIYRKLNDI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEHHHHCC
PANWGTAVNVQSMVFGNLGDTSATGVAFTRDPSNGSPIFYGEYLINAQGEDVVAGIRTPA
CCCCCCEEEEHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCC
PISRERANTLGSSDAPLEEAMPPVYKQLVDVANTLERHYKDMQDIEFTVEDGTLYMLQTR
CCCHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHCEEEEECCEEEEEEEC
NGKRTAAAAVKIAVDMVREGLITEEEALLRIDPTALDQLLHPRIPSDDENKKAGNAAYDV
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCCCEEEE
AFKGLPASPGAAVGRVVFDSEEAFALAAKGEDVILVRVETSPDDIKGMHAARAIVTARGG
EECCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHEECCC
MTSHAAVVARGMGRPCVCGASDLRINTAKGEFTARGRTFKKGDMITVDGVNGRVIAGAVS
CCHHHHHHHHCCCCCEEECCCCCEEEECCCCEEECCCEECCCCEEEEECCCCEEEEEEHH
LINPDLSGDFGEFMGWADKRRTLKVRANAETPLDTRTAVEFGAEGVGLCRTEHMFFDEER
HHCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCC
IPAVRTMILSNDETGRREALAKLLPMQRSDFEEIFRILGDRPATIRLLDPPLHEFLPHTD
CCHHHHEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHCCCCC
EDIAGVAKASGLSAEELRRRAADLHESNPMLGHRGCRLGITYPEIYEMQARAIFEAAVNI
CHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
FKEGKLKPVPEVMIPLVATHRELDILKKLVDETAQKVFAETGVTLDYMVGTMIELPRAAL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AAGEIAQAAEFFSFGTNDLTQTTLGISRDDAGKFLPIYSEKGIYEKDPFVTLDQEGVGEL
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHHHHH
VKIAAERGRKTRPDIKLGICGEHGGDPASVEFCHRAGLDYVSCSPYRVPIARLAAAQAAI
HHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RGAKK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]