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The map label for this gene is leuA [H]

Identifier: 114797728

GI number: 114797728

Start: 436088

End: 437647

Strand: Reverse

Name: leuA [H]

Synonym: HNE_0440

Alternate gene names: 114797728

Gene position: 437647-436088 (Counterclockwise)

Preceding gene: 114798677

Following gene: 114799180

Centisome position: 11.81

GC content: 61.03

Gene sequence:

>1560_bases
ATGACCTCCAAGAACACCGCCGCGAACGACGCAACCAAACCCCGTATCCTGATATTCGACACAACGATGCGCGATGGCGA
GCAATCGCCAGGCGCCTCGATGACGCTGAACGAGAAGCTTGAGCTGGCAACCCTGCTCGAAAGCATGGGCGTCGATATTA
TCGAAGCCGGCTTCCCCGCCGCCTCGGAAGGTGACTTTGCCGCCGTCAGCGCAATTGCCGCCCAGTCCAAGAGCGCCGTG
ATCTGCGGACTTTCCCGCTCGACGCCGAACGACATTGACCGCTGCGGCGAGGCTGTGCGCAAGGCCGCCCGCCCGCGCAT
TCACACCTTCATTTCCACCAGCCCCGTGCACATGAAGCACAAGCTGAAGATGGGCCCGAACGCTGTGCTGGAAGCGGTCG
GCCGCTCGGTCTCTCAGGCCCGCAACCTGGTCGATGATGTGGAGTGGAGCGCCGAAGACGCGACGCGCACCGAATTCGAT
TTCCTCTGCAAATGCATCGACACCGCGATCGCTTCGGGCGCTACCACCATCAACGTGCCTGATACGGTGGGCTATTCCCA
CCCCGACGAATATGGCGCACTGATCCGCCGACTGATCGAAAACATTCCGAACTCGGACAAGGTGATCTGGTCGGCGCACT
GTCATAACGATCTTGGTCTTGCCGTTGCCAACTCGCTGGCCGGCATGGCCAATGGCGTGCGCCAGATCGAGTGCACCATC
AACGGCCTAGGCGAGCGCGCGGGTAATGCGGCGCTTGAAGAGATCGTCATGGCGATGAAGGTGCGCGGCGACACCCTGCC
CTATGAAACCCATATCGATACCAGCTATCTCGCCCGCGCCTCGGCAATGGTCAGCCGCATCACGGGCTTCCCGGTGCAGT
ATAACAAGGCCATCGTTGGCAAGAATGCCTTCGCGCATGAAAGCGGCATCCACCAGGATGGCATGCTGAAGAATTCCGAG
ACCTACGAGATCATGAAGCCCGAAGATGTGGGCGTGGCGAAAACCTCGCTCGTCATGGGCAAGCTCTCCGGGCGCAACGC
TTTCCGCGACAAGCTGGAATCGATGGGCTATTTCATCGAGGGCGACGCGCTGAACGACGCGTTCAAGCGCTTCAAGGATC
TGGCCGACCGCAAGAAACATGTGTTCGATGACGACATCATCGCGCTGGTGGATGAGCAGCTGGCGGCAGAAGGCGAGCGG
ATCGCGTTCAAGCGGCTGCGCGTTGTGGCAGGCACGGAAGGTCCGCAGACGGCAGAAATCGACCTGGTGATTGACGGCGA
GGAGAAATACTCCATCGCGCGCGGCAATGGCCCGGTAGACGCCGTGTTCAATGCCATTCGCCAGCTGGTGCCGCATCAGG
CCATTCTGGAGCTTTACCAGGTGCATGCCGTGACCGGCGGTACGGACGCGCAGGCAACTGTGTCTGTGCGGCTGAATGGC
GATGACATCATGGCGACGGGCCGGTCATCCGATCCCGACACGCTGGTGGCCAGCGCCAAGGCCTATCTGCACGCGCTGAA
CAAGTTTGAAGTGCGCAAGGCAAAGCGCAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGGGGACGGACACCCAAGTCTGAGGGTGGACGCGCGACCAGGGGATATGAGACCAAGAACCGCTTTGTTCCTTCCTCT
CCGCTGAGCTACGCAAAACC

Downstream 100 bases:

>100_bases
AGCGGGCTGATCAGCCCCCGTCACGTTCCCCGAAGCCATCGGCGACCAGCGCGGCGATGGCTTCAACCGCCTCTGCGGCC
TGCGTGCCGGTGCCCGAAAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTSKNTAANDATKPRILIFDTTMRDGEQSPGASMTLNEKLELATLLESMGVDIIEAGFPAASEGDFAAVSAIAAQSKSAV
ICGLSRSTPNDIDRCGEAVRKAARPRIHTFISTSPVHMKHKLKMGPNAVLEAVGRSVSQARNLVDDVEWSAEDATRTEFD
FLCKCIDTAIASGATTINVPDTVGYSHPDEYGALIRRLIENIPNSDKVIWSAHCHNDLGLAVANSLAGMANGVRQIECTI
NGLGERAGNAALEEIVMAMKVRGDTLPYETHIDTSYLARASAMVSRITGFPVQYNKAIVGKNAFAHESGIHQDGMLKNSE
TYEIMKPEDVGVAKTSLVMGKLSGRNAFRDKLESMGYFIEGDALNDAFKRFKDLADRKKHVFDDDIIALVDEQLAAEGER
IAFKRLRVVAGTEGPQTAEIDLVIDGEEKYSIARGNGPVDAVFNAIRQLVPHQAILELYQVHAVTGGTDAQATVSVRLNG
DDIMATGRSSDPDTLVASAKAYLHALNKFEVRKAKRKAA

Sequences:

>Translated_519_residues
MTSKNTAANDATKPRILIFDTTMRDGEQSPGASMTLNEKLELATLLESMGVDIIEAGFPAASEGDFAAVSAIAAQSKSAV
ICGLSRSTPNDIDRCGEAVRKAARPRIHTFISTSPVHMKHKLKMGPNAVLEAVGRSVSQARNLVDDVEWSAEDATRTEFD
FLCKCIDTAIASGATTINVPDTVGYSHPDEYGALIRRLIENIPNSDKVIWSAHCHNDLGLAVANSLAGMANGVRQIECTI
NGLGERAGNAALEEIVMAMKVRGDTLPYETHIDTSYLARASAMVSRITGFPVQYNKAIVGKNAFAHESGIHQDGMLKNSE
TYEIMKPEDVGVAKTSLVMGKLSGRNAFRDKLESMGYFIEGDALNDAFKRFKDLADRKKHVFDDDIIALVDEQLAAEGER
IAFKRLRVVAGTEGPQTAEIDLVIDGEEKYSIARGNGPVDAVFNAIRQLVPHQAILELYQVHAVTGGTDAQATVSVRLNG
DDIMATGRSSDPDTLVASAKAYLHALNKFEVRKAKRKAA
>Mature_518_residues
TSKNTAANDATKPRILIFDTTMRDGEQSPGASMTLNEKLELATLLESMGVDIIEAGFPAASEGDFAAVSAIAAQSKSAVI
CGLSRSTPNDIDRCGEAVRKAARPRIHTFISTSPVHMKHKLKMGPNAVLEAVGRSVSQARNLVDDVEWSAEDATRTEFDF
LCKCIDTAIASGATTINVPDTVGYSHPDEYGALIRRLIENIPNSDKVIWSAHCHNDLGLAVANSLAGMANGVRQIECTIN
GLGERAGNAALEEIVMAMKVRGDTLPYETHIDTSYLARASAMVSRITGFPVQYNKAIVGKNAFAHESGIHQDGMLKNSET
YEIMKPEDVGVAKTSLVMGKLSGRNAFRDKLESMGYFIEGDALNDAFKRFKDLADRKKHVFDDDIIALVDEQLAAEGERI
AFKRLRVVAGTEGPQTAEIDLVIDGEEKYSIARGNGPVDAVFNAIRQLVPHQAILELYQVHAVTGGTDAQATVSVRLNGD
DIMATGRSSDPDTLVASAKAYLHALNKFEVRKAKRKAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=45.0292397660819, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=26.2162162162162, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320071, Length=370, Percent_Identity=26.2162162162162, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=532, Percent_Identity=25.9398496240602, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=525, Percent_Identity=24.7619047619048, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56021; Mature: 55890

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKNTAANDATKPRILIFDTTMRDGEQSPGASMTLNEKLELATLLESMGVDIIEAGFPA
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCC
ASEGDFAAVSAIAAQSKSAVICGLSRSTPNDIDRCGEAVRKAARPRIHTFISTSPVHMKH
CCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
KLKMGPNAVLEAVGRSVSQARNLVDDVEWSAEDATRTEFDFLCKCIDTAIASGATTINVP
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECC
DTVGYSHPDEYGALIRRLIENIPNSDKVIWSAHCHNDLGLAVANSLAGMANGVRQIECTI
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
NGLGERAGNAALEEIVMAMKVRGDTLPYETHIDTSYLARASAMVSRITGFPVQYNKAIVG
EHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEEC
KNAFAHESGIHQDGMLKNSETYEIMKPEDVGVAKTSLVMGKLSGRNAFRDKLESMGYFIE
CCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCEEEE
GDALNDAFKRFKDLADRKKHVFDDDIIALVDEQLAAEGERIAFKRLRVVAGTEGPQTAEI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCEEE
DLVIDGEEKYSIARGNGPVDAVFNAIRQLVPHQAILELYQVHAVTGGTDAQATVSVRLNG
EEEEECCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEECC
DDIMATGRSSDPDTLVASAKAYLHALNKFEVRKAKRKAA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSKNTAANDATKPRILIFDTTMRDGEQSPGASMTLNEKLELATLLESMGVDIIEAGFPA
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCC
ASEGDFAAVSAIAAQSKSAVICGLSRSTPNDIDRCGEAVRKAARPRIHTFISTSPVHMKH
CCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
KLKMGPNAVLEAVGRSVSQARNLVDDVEWSAEDATRTEFDFLCKCIDTAIASGATTINVP
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECC
DTVGYSHPDEYGALIRRLIENIPNSDKVIWSAHCHNDLGLAVANSLAGMANGVRQIECTI
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
NGLGERAGNAALEEIVMAMKVRGDTLPYETHIDTSYLARASAMVSRITGFPVQYNKAIVG
EHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEEC
KNAFAHESGIHQDGMLKNSETYEIMKPEDVGVAKTSLVMGKLSGRNAFRDKLESMGYFIE
CCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCEEEE
GDALNDAFKRFKDLADRKKHVFDDDIIALVDEQLAAEGERIAFKRLRVVAGTEGPQTAEI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCEEE
DLVIDGEEKYSIARGNGPVDAVFNAIRQLVPHQAILELYQVHAVTGGTDAQATVSVRLNG
EEEEECCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEECC
DDIMATGRSSDPDTLVASAKAYLHALNKFEVRKAKRKAA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA