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The map label for this gene is sseA [H]

Identifier: 114797517

GI number: 114797517

Start: 1448282

End: 1449124

Strand: Reverse

Name: sseA [H]

Synonym: HNE_1419

Alternate gene names: 114797517

Gene position: 1449124-1448282 (Counterclockwise)

Preceding gene: 114800279

Following gene: 114798688

Centisome position: 39.11

GC content: 64.65

Gene sequence:

>843_bases
ATGAGCGAGAGCGGCGACCCGCTGGTCAGTGCAGACTGGCTGATGGCGAATATCAGCGCGCCGGATGTGCGCATTGTGGA
CGCGACCTGGTTTGCGCCCTGGACCGACCCGCCCGAAACGGCACATGACCTGTGGAAACGCGGGCATATTCCGAAGGCGG
TCTATTTCGACATCGACGATATTGCCGATCTCAATTCCCCCTACCCGCACATGCTACCGGATGCCGTGAAGTTTTCCTCA
CGCGTGCGCAAGCTGGGCCTGGGCGATGGAAGCCGGATTGTTGTGTATGACCAGAACCGGTTTTTCGCCTCGGCGCGCGC
CTGGTGGATGCTGCGCGTGATGGGGCACAAGGATGTGCATGTGCTCGATGGCGGACTGCATGCCTGGGTGACCGAAGGCG
GCGCGCTGGACGATCTGCCGACACCGCCCGGCGAGCGCCACTTCACCCCGCGCGTGCGCAGCGACCTGATCATGGACGCG
CGCGGCATGGAACAGCTGGTGGGCTCTGCGCGTGTACGGATCATAGATGCCCGGCCCGATGGACGCTTCTTTGGCCGCGA
TCCCGAGCCACGCCCCGGCATGCCTTCCGGACACATTCCCGGAAGCATCAATGTGCCGGGTAACCTGCTGGTGACCGAGG
ACGGGCGGATGAAATCTGCCAGCGAACTGGCCCGGCTGATCCCCGAGCCCGCAAAACCCACCGTTGCCACCTGCGGCTCT
GGTGTAACCGCGGCCATCACGGCGCTGGCGCTGGCGCGCCTCGGAAACTGGGACGTCGCCGTTTATGACGGCTCGTGGAC
CGAATGGGTGTCTGACCCCGCCCGGCCAATCGCGAAAGGCTGA

Upstream 100 bases:

>100_bases
TCCTCTGTGACTACGGCAACCGGTATCAGACGAAATTGTTCAACCCGGAATTCCTGCGCTCCAAAGGCCTGCCGGTTCCG
TCCTGGATGGAGGCGAAATA

Downstream 100 bases:

>100_bases
AGCGATGAGCGCACGGGATGACGCCGAGCCCCAGACCCGCCTGATCCACACCCGCGCCGAACGCCTTGGCCGGGTGACCG
TGAACCCGCCGGTGGAGCGT

Product: putative sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSESGDPLVSADWLMANISAPDVRIVDATWFAPWTDPPETAHDLWKRGHIPKAVYFDIDDIADLNSPYPHMLPDAVKFSS
RVRKLGLGDGSRIVVYDQNRFFASARAWWMLRVMGHKDVHVLDGGLHAWVTEGGALDDLPTPPGERHFTPRVRSDLIMDA
RGMEQLVGSARVRIIDARPDGRFFGRDPEPRPGMPSGHIPGSINVPGNLLVTEDGRMKSASELARLIPEPAKPTVATCGS
GVTAAITALALARLGNWDVAVYDGSWTEWVSDPARPIAKG

Sequences:

>Translated_280_residues
MSESGDPLVSADWLMANISAPDVRIVDATWFAPWTDPPETAHDLWKRGHIPKAVYFDIDDIADLNSPYPHMLPDAVKFSS
RVRKLGLGDGSRIVVYDQNRFFASARAWWMLRVMGHKDVHVLDGGLHAWVTEGGALDDLPTPPGERHFTPRVRSDLIMDA
RGMEQLVGSARVRIIDARPDGRFFGRDPEPRPGMPSGHIPGSINVPGNLLVTEDGRMKSASELARLIPEPAKPTVATCGS
GVTAAITALALARLGNWDVAVYDGSWTEWVSDPARPIAKG
>Mature_279_residues
SESGDPLVSADWLMANISAPDVRIVDATWFAPWTDPPETAHDLWKRGHIPKAVYFDIDDIADLNSPYPHMLPDAVKFSSR
VRKLGLGDGSRIVVYDQNRFFASARAWWMLRVMGHKDVHVLDGGLHAWVTEGGALDDLPTPPGERHFTPRVRSDLIMDAR
GMEQLVGSARVRIIDARPDGRFFGRDPEPRPGMPSGHIPGSINVPGNLLVTEDGRMKSASELARLIPEPAKPTVATCGSG
VTAAITALALARLGNWDVAVYDGSWTEWVSDPARPIAKG

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=38.2352941176471, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=38.2352941176471, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=38.2352941176471, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=38.8278388278388, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI87082121, Length=266, Percent_Identity=39.0977443609023, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI87081967, Length=277, Percent_Identity=26.3537906137184, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17561888, Length=295, Percent_Identity=27.7966101694915, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71997283, Length=300, Percent_Identity=26.3333333333333, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17559150, Length=304, Percent_Identity=24.3421052631579, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543836, Length=280, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543838, Length=280, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=234, Percent_Identity=25.2136752136752, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324825, Length=268, Percent_Identity=37.3134328358209, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30615; Mature: 30484

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESGDPLVSADWLMANISAPDVRIVDATWFAPWTDPPETAHDLWKRGHIPKAVYFDIDD
CCCCCCCEEECCEEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCHH
IADLNSPYPHMLPDAVKFSSRVRKLGLGDGSRIVVYDQNRFFASARAWWMLRVMGHKDVH
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHEEEEEECCCCCEE
VLDGGLHAWVTEGGALDDLPTPPGERHFTPRVRSDLIMDARGMEQLVGSARVRIIDARPD
EEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCEEEEEECCCC
GRFFGRDPEPRPGMPSGHIPGSINVPGNLLVTEDGRMKSASELARLIPEPAKPTVATCGS
CCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHCCCCCCCCHHHCCC
GVTAAITALALARLGNWDVAVYDGSWTEWVSDPARPIAKG
CHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SESGDPLVSADWLMANISAPDVRIVDATWFAPWTDPPETAHDLWKRGHIPKAVYFDIDD
CCCCCCEEECCEEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCHH
IADLNSPYPHMLPDAVKFSSRVRKLGLGDGSRIVVYDQNRFFASARAWWMLRVMGHKDVH
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHEEEEEECCCCCEE
VLDGGLHAWVTEGGALDDLPTPPGERHFTPRVRSDLIMDARGMEQLVGSARVRIIDARPD
EEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCEEEEEECCCC
GRFFGRDPEPRPGMPSGHIPGSINVPGNLLVTEDGRMKSASELARLIPEPAKPTVATCGS
CCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHCCCCCCCCHHHCCC
GVTAAITALALARLGNWDVAVYDGSWTEWVSDPARPIAKG
CHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]