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The map label for this gene is rfbD [H]

Identifier: 114797456

GI number: 114797456

Start: 807479

End: 808453

Strand: Direct

Name: rfbD [H]

Synonym: HNE_0794

Alternate gene names: 114797456

Gene position: 807479-808453 (Clockwise)

Preceding gene: 114798249

Following gene: 114800250

Centisome position: 21.79

GC content: 56.31

Gene sequence:

>975_bases
ATGACAGGTCGCCGTATCCTCATCACAGGCGCAAATGGCTTTGTCGGCGGATATATGATCCGCGAACTTGTCGCAGCAGG
TTATCAGCATTCAGAGTTGCACACATTAACAGTTTCGGGAGCTGAGCAGTCTCTGAGAATAGGGCAGGCTCATCGGTGCG
ATCTCAGGGATAGCGCTTCGATCCACCGTCTGCTGCAGGAGGTGCAGCCTACAGGAATTGTGCACCTTGCTGCGATTGCT
CTGCCTTCTCAAGCCAAGGCTGATCCCTCTGCGGCCTGGGCTGTGAATTTTGAAGCTGTGCGCCAGTTGGGGGAGGCGGT
TCTGGCTTGTTCGCCACATGCCGTTCTCGTATTTGCAGGCAGCTCCGAAAGCTATGGTGCAAGCTTCAATACTGTTGCCG
GGGCAGTCAATGAGGGGACCGCGCTGCGCCCTCTGACACCCTATGCAGCGACCAAGGCGGCGGCGGATGTTGCGTTGGGG
CAGATGCGTAATGACGGCCTGAACGCAGTACGTTTCCGGGCCTTCAATCACACGGGGCCAGGCCAATCGCCGGACTATGT
CGTTGCAAGTTTTGCCGCTCAGATTGCCCAGATAATTGCGGGTGATCATCCCCCGGTTATTCGCGTAGGCAATCTTGATG
CAATGCGGGATTTCGTGGATGTTCGAGATGTTGTGCGCGGCTACAGGCTTGCTCTTGAGACGGAGCTTGATCCGGTCTCT
GAGGGAGTATTCAATCTCGCATCGGGCACGCCTCGTTCCATCCGCTCGATACTCAACACGTTAATAGACATTGCTGGCGT
AGACATTGCAATCGAGACAGATCCGGCAAAGTTGCGCAAGAATGACGTACCGCGGACATGGGGAGATGCTAACCGGGCTC
GCACGGAGCTGGGCTGGGTCCCATATCTTGCCTTCGAACAGACCTTGGTCGATACCCTCTCCAGTGCGGGTGCCAAAATT
TCCGGCTGCACCTAG

Upstream 100 bases:

>100_bases
CGGAAATTTCGCTTGAAAAGCTCATCCGTGAGATGGTCGATGCGGATATGGAACGGCTGAAGCCGTAAACGGTTCAGGCT
GACAGAAAGGCATCTGCGAA

Downstream 100 bases:

>100_bases
ACGTTGGTTTGGAACGTTGGGAAATTTTCGGTCGTTTTCTTCCAGAGTCGGTTAGAGAGGTAGCAAGTTGGAGCAACAGA
TTCAGTCGGCTTGGAACTAT

Product: putative GDP-6-deoxy-D-lyxo-4-hexulose reductase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTGRRILITGANGFVGGYMIRELVAAGYQHSELHTLTVSGAEQSLRIGQAHRCDLRDSASIHRLLQEVQPTGIVHLAAIA
LPSQAKADPSAAWAVNFEAVRQLGEAVLACSPHAVLVFAGSSESYGASFNTVAGAVNEGTALRPLTPYAATKAAADVALG
QMRNDGLNAVRFRAFNHTGPGQSPDYVVASFAAQIAQIIAGDHPPVIRVGNLDAMRDFVDVRDVVRGYRLALETELDPVS
EGVFNLASGTPRSIRSILNTLIDIAGVDIAIETDPAKLRKNDVPRTWGDANRARTELGWVPYLAFEQTLVDTLSSAGAKI
SGCT

Sequences:

>Translated_324_residues
MTGRRILITGANGFVGGYMIRELVAAGYQHSELHTLTVSGAEQSLRIGQAHRCDLRDSASIHRLLQEVQPTGIVHLAAIA
LPSQAKADPSAAWAVNFEAVRQLGEAVLACSPHAVLVFAGSSESYGASFNTVAGAVNEGTALRPLTPYAATKAAADVALG
QMRNDGLNAVRFRAFNHTGPGQSPDYVVASFAAQIAQIIAGDHPPVIRVGNLDAMRDFVDVRDVVRGYRLALETELDPVS
EGVFNLASGTPRSIRSILNTLIDIAGVDIAIETDPAKLRKNDVPRTWGDANRARTELGWVPYLAFEQTLVDTLSSAGAKI
SGCT
>Mature_323_residues
TGRRILITGANGFVGGYMIRELVAAGYQHSELHTLTVSGAEQSLRIGQAHRCDLRDSASIHRLLQEVQPTGIVHLAAIAL
PSQAKADPSAAWAVNFEAVRQLGEAVLACSPHAVLVFAGSSESYGASFNTVAGAVNEGTALRPLTPYAATKAAADVALGQ
MRNDGLNAVRFRAFNHTGPGQSPDYVVASFAAQIAQIIAGDHPPVIRVGNLDAMRDFVDVRDVVRGYRLALETELDPVSE
GVFNLASGTPRSIRSILNTLIDIAGVDIAIETDPAKLRKNDVPRTWGDANRARTELGWVPYLAFEQTLVDTLSSAGAKIS
GCT

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=344, Percent_Identity=26.7441860465116, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=24.7159090909091, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=24.5714285714286, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786974, Length=332, Percent_Identity=22.5903614457831, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71982035, Length=342, Percent_Identity=25.1461988304094, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=24.0687679083095, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=352, Percent_Identity=25, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=349, Percent_Identity=25.214899713467, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=349, Percent_Identity=25.214899713467, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=352, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=352, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=347, Percent_Identity=24.7838616714697, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=341, Percent_Identity=23.4604105571848, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=24.9283667621776, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 34380; Mature: 34249

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRRILITGANGFVGGYMIRELVAAGYQHSELHTLTVSGAEQSLRIGQAHRCDLRDSAS
CCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCHH
IHRLLQEVQPTGIVHLAAIALPSQAKADPSAAWAVNFEAVRQLGEAVLACSPHAVLVFAG
HHHHHHHHCCCCCEEEHEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEC
SSESYGASFNTVAGAVNEGTALRPLTPYAATKAAADVALGQMRNDGLNAVRFRAFNHTGP
CCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GQSPDYVVASFAAQIAQIIAGDHPPVIRVGNLDAMRDFVDVRDVVRGYRLALETELDPVS
CCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCHHH
EGVFNLASGTPRSIRSILNTLIDIAGVDIAIETDPAKLRKNDVPRTWGDANRARTELGWV
HHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCHHHHHHHCCC
PYLAFEQTLVDTLSSAGAKISGCT
HHHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure 
TGRRILITGANGFVGGYMIRELVAAGYQHSELHTLTVSGAEQSLRIGQAHRCDLRDSAS
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCHH
IHRLLQEVQPTGIVHLAAIALPSQAKADPSAAWAVNFEAVRQLGEAVLACSPHAVLVFAG
HHHHHHHHCCCCCEEEHEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEC
SSESYGASFNTVAGAVNEGTALRPLTPYAATKAAADVALGQMRNDGLNAVRFRAFNHTGP
CCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GQSPDYVVASFAAQIAQIIAGDHPPVIRVGNLDAMRDFVDVRDVVRGYRLALETELDPVS
CCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCHHH
EGVFNLASGTPRSIRSILNTLIDIAGVDIAIETDPAKLRKNDVPRTWGDANRARTELGWV
HHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCHHHHHHHCCC
PYLAFEQTLVDTLSSAGAKISGCT
HHHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]