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The map label for this gene is gmd [H]

Identifier: 114798249

GI number: 114798249

Start: 806469

End: 807446

Strand: Direct

Name: gmd [H]

Synonym: HNE_0793

Alternate gene names: 114798249

Gene position: 806469-807446 (Clockwise)

Preceding gene: 114798947

Following gene: 114797456

Centisome position: 21.77

GC content: 54.91

Gene sequence:

>978_bases
ATGACGCGTAAGACCGCTTTCATCACAGGCATAACCGGGCAGGACGGGGCTTATCTGTCCGCGTTGCTGCTAAGTAAAGG
CTACCGGGTGACCGGACTTGTTCGCAGGTCTTCAACGGCTGAAATCAACAACTTGCGCCTGCGCTGGCTTGGTATCGAGA
ACGATGTCGAATTGATCGACGGCGACCTGACGGATCTTTCTGGACTGTTGCGTTCTGTCAAAGAAATCCGGCCCGATGAA
ATTTACAACCTGGGTGCCCAGTCCTTTGTGAAATCGTCTTGGCAGCAGCCCATCCTGACGGGGCAGGTGACGGGGCTTGC
GGTCACCAATATTCTTGAGGCTATGCGCATCGAAGCGCCGGAGGCGCGTCTCTATCAGGCATCGTCCTCCGAGATGTACG
GCCTTATCCAAGAAGACATGCAGTCGGAGACGACACCATTCTATCCCCGGTCGCCTTACGCCGTCGCTAAGCTGTATGGG
CATTGGATCACCGTGAACTATCGCGAGAGCTTCGGTTTGCACGCTTCCAGCGGTATCTTGTTCAACCACGAAAGCCCGCT
GCGCGGAATCGAGTTCGTGACACGAAAAGTGACTGATGGAGTTGCCCGAATCAAACTTGGCCTCTCCAGCGAGCTTCGCC
TTGGAAACATCGATGCCAAGCGCGATTGGGGCCACGCAAAGGATTATGTTCGGGCAATGTGGTTGATGTTGCAGCAGGAA
ACGCCAGACGATTACGTGGTGGCCACCGGTGTAACCACGACCGTTCGTGATATGTGCCTTATCGCGTTCGATCATGTTGG
CCTTGAGATTGACAAGCACTTAGTGATCGACCCCGCACTTTTTCGTCCGGCCGAGGTGGACGTGTTGCTTGGCAATCCGG
CCAAGGCAAAGGCAAAATTGGGGTGGGAGCCGGAAATTTCGCTTGAAAAGCTCATCCGTGAGATGGTCGATGCGGATATG
GAACGGCTGAAGCCGTAA

Upstream 100 bases:

>100_bases
CTAATGGACTTGGATTATCAGTCGTTTTCAACCCTGTGTCTCAGGCTTGACAAGGACTGAGTGGCCGCTGACAACGGCTC
AAAATTGATCGGGATCAACC

Downstream 100 bases:

>100_bases
ACGGTTCAGGCTGACAGAAAGGCATCTGCGAAATGACAGGTCGCCGTATCCTCATCACAGGCGCAAATGGCTTTGTCGGC
GGATATATGATCCGCGAACT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTRKTAFITGITGQDGAYLSALLLSKGYRVTGLVRRSSTAEINNLRLRWLGIENDVELIDGDLTDLSGLLRSVKEIRPDE
IYNLGAQSFVKSSWQQPILTGQVTGLAVTNILEAMRIEAPEARLYQASSSEMYGLIQEDMQSETTPFYPRSPYAVAKLYG
HWITVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGLSSELRLGNIDAKRDWGHAKDYVRAMWLMLQQE
TPDDYVVATGVTTTVRDMCLIAFDHVGLEIDKHLVIDPALFRPAEVDVLLGNPAKAKAKLGWEPEISLEKLIREMVDADM
ERLKP

Sequences:

>Translated_325_residues
MTRKTAFITGITGQDGAYLSALLLSKGYRVTGLVRRSSTAEINNLRLRWLGIENDVELIDGDLTDLSGLLRSVKEIRPDE
IYNLGAQSFVKSSWQQPILTGQVTGLAVTNILEAMRIEAPEARLYQASSSEMYGLIQEDMQSETTPFYPRSPYAVAKLYG
HWITVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGLSSELRLGNIDAKRDWGHAKDYVRAMWLMLQQE
TPDDYVVATGVTTTVRDMCLIAFDHVGLEIDKHLVIDPALFRPAEVDVLLGNPAKAKAKLGWEPEISLEKLIREMVDADM
ERLKP
>Mature_324_residues
TRKTAFITGITGQDGAYLSALLLSKGYRVTGLVRRSSTAEINNLRLRWLGIENDVELIDGDLTDLSGLLRSVKEIRPDEI
YNLGAQSFVKSSWQQPILTGQVTGLAVTNILEAMRIEAPEARLYQASSSEMYGLIQEDMQSETTPFYPRSPYAVAKLYGH
WITVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGLSSELRLGNIDAKRDWGHAKDYVRAMWLMLQQET
PDDYVVATGVTTTVRDMCLIAFDHVGLEIDKHLVIDPALFRPAEVDVLLGNPAKAKAKLGWEPEISLEKLIREMVDADME
RLKP

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=347, Percent_Identity=48.9913544668588, Blast_Score=332, Evalue=3e-91,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=49.5750708215297, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=49.8542274052478, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17539424, Length=345, Percent_Identity=48.4057971014493, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI133901790, Length=345, Percent_Identity=48.4057971014493, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17539422, Length=345, Percent_Identity=48.4057971014493, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI133901786, Length=345, Percent_Identity=48.4057971014493, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI133901788, Length=345, Percent_Identity=48.4057971014493, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24158427, Length=346, Percent_Identity=47.1098265895954, Blast_Score=316, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36383; Mature: 36252

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKTAFITGITGQDGAYLSALLLSKGYRVTGLVRRSSTAEINNLRLRWLGIENDVELID
CCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEC
GDLTDLSGLLRSVKEIRPDEIYNLGAQSFVKSSWQQPILTGQVTGLAVTNILEAMRIEAP
CCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCC
EARLYQASSSEMYGLIQEDMQSETTPFYPRSPYAVAKLYGHWITVNYRESFGLHASSGIL
HHHEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEHHCCCCCCCCCEE
FNHESPLRGIEFVTRKVTDGVARIKLGLSSELRLGNIDAKRDWGHAKDYVRAMWLMLQQE
ECCCCCCHHHHHHHHHHHCCHHEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCC
TPDDYVVATGVTTTVRDMCLIAFDHVGLEIDKHLVIDPALFRPAEVDVLLGNPAKAKAKL
CCCCEEEECCCHHHHHHHHHHHHHHCCCEECCEEEECHHHCCCCEEEEEECCCCCCCCCC
GWEPEISLEKLIREMVDADMERLKP
CCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRKTAFITGITGQDGAYLSALLLSKGYRVTGLVRRSSTAEINNLRLRWLGIENDVELID
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEC
GDLTDLSGLLRSVKEIRPDEIYNLGAQSFVKSSWQQPILTGQVTGLAVTNILEAMRIEAP
CCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCC
EARLYQASSSEMYGLIQEDMQSETTPFYPRSPYAVAKLYGHWITVNYRESFGLHASSGIL
HHHEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEEHHCCCCCCCCCEE
FNHESPLRGIEFVTRKVTDGVARIKLGLSSELRLGNIDAKRDWGHAKDYVRAMWLMLQQE
ECCCCCCHHHHHHHHHHHCCHHEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCC
TPDDYVVATGVTTTVRDMCLIAFDHVGLEIDKHLVIDPALFRPAEVDVLLGNPAKAKAKL
CCCCEEEECCCHHHHHHHHHHHHHHCCCEECCEEEECHHHCCCCEEEEEECCCCCCCCCC
GWEPEISLEKLIREMVDADMERLKP
CCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]