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The map label for this gene is rutA [H]

Identifier: 114797293

GI number: 114797293

Start: 1276194

End: 1277249

Strand: Reverse

Name: rutA [H]

Synonym: HNE_1252

Alternate gene names: 114797293

Gene position: 1277249-1276194 (Counterclockwise)

Preceding gene: 114800339

Following gene: 114798275

Centisome position: 34.47

GC content: 61.74

Gene sequence:

>1056_bases
ATGTTCGGTGTCTTCCTGCCCATCGCCAATGGCGGCTGGATCGTCTCGAAGAACACGCCGACGCTCGACGGCCTGTATGA
AACCAATCGCGAAGCCGCGATCACCGCAGATCGCATCGGCCTCGACTTCATCATGTCCATGGCGAAGTTCCGGGGCTTCG
GCGGGGAAACCAACCACTGGGCCACCTCCATGGAATCCATGACCATGATGGCCGGCCTCGCCGAAGCCACCAAGACTGTG
AAGGTCTGGGCTACCGTTCACGCGCTCCTTTCCAATCCCGCCGTCGTCGCCAAGATGGTCACAACGCTCGATCATATCTC
CAAGGGCCGCGCGGGCCTCAACATCGTCGCCGGCGCCTACCGCGAGGAATTCTCCCAGATGGGCGCCTGGGACGATAGCC
TCTCCCATGGCGACCGTTATCAGCTCACGGAAGAATGGATCACCATCATCAAGCGCCTCTGGTCAGAGCCGAGCGTCACC
TTCAAGGGCCGCTTCTTCGATATGGAAGACTGCCAGTCTGATCCCAAACCCATCTCCCGGCCCCGGCCAGACCTGATCGC
GGCAGGCCAGTCAGATCGGGGCTTTGACTTCTCAACCACCCATGCCGATGCCTGCTTCATCGGCGGGCGCAGCGTCGAGG
AGCGCCGGGGCCACAGCCGCCGCGCCCGCGAGATCGCCGCGGAGAAAGGCAAGACCATCAAGGTTTGCGCCATGTGTACC
GTCATCCACGCCGACACCGACGCCAAAGCCGAATCCATGCTGCAACGCTTCGAAGACGGGGCCGATCTTGGCGCCATCAT
GGCCCAGATGAAAAGCTGGGGCATCGCGCCCGACAGCCTCGCCGCCATGGCCCGCAAGGTCGGCGTCAGCCAGAACCATT
CCGTCGTCGGATCGCCAGAGACGTGCCGCGAGAAGATCGAGGAATATATGGAAACCTGCGATCTTGACGGCGTGATGCTG
ATTTTCCCCGACTACAAGGTCGGTCTGGAAATGTTCGGAGACATTCTCCCCAAGCTGCGCCAGCGCTTTCCCGCCCAGAA
CCACGAACCCGCATAA

Upstream 100 bases:

>100_bases
ACACCGCCCCCGACACGGGCCGCGATGCGCCCGCCATCGCTGAACGAACCGCTTAATTTCCAATACGTGGAGCCCAATAA
CCATGTTGCCCAATCATAAA

Downstream 100 bases:

>100_bases
TCAGATAACAGAATAAGGGAGAGGGAAACCTCATGGCTGACGGACTGCTGGACAGCGCGCTGGGCGCCGGGGTATCCCCG
GAAGACGTAAAGGCAATCGC

Product: luciferase family monooxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MFGVFLPIANGGWIVSKNTPTLDGLYETNREAAITADRIGLDFIMSMAKFRGFGGETNHWATSMESMTMMAGLAEATKTV
KVWATVHALLSNPAVVAKMVTTLDHISKGRAGLNIVAGAYREEFSQMGAWDDSLSHGDRYQLTEEWITIIKRLWSEPSVT
FKGRFFDMEDCQSDPKPISRPRPDLIAAGQSDRGFDFSTTHADACFIGGRSVEERRGHSRRAREIAAEKGKTIKVCAMCT
VIHADTDAKAESMLQRFEDGADLGAIMAQMKSWGIAPDSLAAMARKVGVSQNHSVVGSPETCREKIEEYMETCDLDGVML
IFPDYKVGLEMFGDILPKLRQRFPAQNHEPA

Sequences:

>Translated_351_residues
MFGVFLPIANGGWIVSKNTPTLDGLYETNREAAITADRIGLDFIMSMAKFRGFGGETNHWATSMESMTMMAGLAEATKTV
KVWATVHALLSNPAVVAKMVTTLDHISKGRAGLNIVAGAYREEFSQMGAWDDSLSHGDRYQLTEEWITIIKRLWSEPSVT
FKGRFFDMEDCQSDPKPISRPRPDLIAAGQSDRGFDFSTTHADACFIGGRSVEERRGHSRRAREIAAEKGKTIKVCAMCT
VIHADTDAKAESMLQRFEDGADLGAIMAQMKSWGIAPDSLAAMARKVGVSQNHSVVGSPETCREKIEEYMETCDLDGVML
IFPDYKVGLEMFGDILPKLRQRFPAQNHEPA
>Mature_351_residues
MFGVFLPIANGGWIVSKNTPTLDGLYETNREAAITADRIGLDFIMSMAKFRGFGGETNHWATSMESMTMMAGLAEATKTV
KVWATVHALLSNPAVVAKMVTTLDHISKGRAGLNIVAGAYREEFSQMGAWDDSLSHGDRYQLTEEWITIIKRLWSEPSVT
FKGRFFDMEDCQSDPKPISRPRPDLIAAGQSDRGFDFSTTHADACFIGGRSVEERRGHSRRAREIAAEKGKTIKVCAMCT
VIHADTDAKAESMLQRFEDGADLGAIMAQMKSWGIAPDSLAAMARKVGVSQNHSVVGSPETCREKIEEYMETCDLDGVML
IFPDYKVGLEMFGDILPKLRQRFPAQNHEPA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787247, Length=364, Percent_Identity=34.8901098901099, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787166, Length=351, Percent_Identity=23.3618233618234, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: NA

Molecular weight: Translated: 38770; Mature: 38770

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGVFLPIANGGWIVSKNTPTLDGLYETNREAAITADRIGLDFIMSMAKFRGFGGETNHW
CCEEEEEECCCCEEEECCCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHH
ATSMESMTMMAGLAEATKTVKVWATVHALLSNPAVVAKMVTTLDHISKGRAGLNIVAGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
REEFSQMGAWDDSLSHGDRYQLTEEWITIIKRLWSEPSVTFKGRFFDMEDCQSDPKPISR
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHCCCCCCCCC
PRPDLIAAGQSDRGFDFSTTHADACFIGGRSVEERRGHSRRAREIAAEKGKTIKVCAMCT
CCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHCHHHHHHHHHHHCCCEEEEEEEEH
VIHADTDAKAESMLQRFEDGADLGAIMAQMKSWGIAPDSLAAMARKVGVSQNHSVVGSPE
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCH
TCREKIEEYMETCDLDGVMLIFPDYKVGLEMFGDILPKLRQRFPAQNHEPA
HHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFGVFLPIANGGWIVSKNTPTLDGLYETNREAAITADRIGLDFIMSMAKFRGFGGETNHW
CCEEEEEECCCCEEEECCCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHH
ATSMESMTMMAGLAEATKTVKVWATVHALLSNPAVVAKMVTTLDHISKGRAGLNIVAGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
REEFSQMGAWDDSLSHGDRYQLTEEWITIIKRLWSEPSVTFKGRFFDMEDCQSDPKPISR
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHCCCCCCCCC
PRPDLIAAGQSDRGFDFSTTHADACFIGGRSVEERRGHSRRAREIAAEKGKTIKVCAMCT
CCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHCHHHHHHHHHHHCCCEEEEEEEEH
VIHADTDAKAESMLQRFEDGADLGAIMAQMKSWGIAPDSLAAMARKVGVSQNHSVVGSPE
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCH
TCREKIEEYMETCDLDGVMLIFPDYKVGLEMFGDILPKLRQRFPAQNHEPA
HHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA