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The map label for this gene is hisS

Identifier: 114332132

GI number: 114332132

Start: 2296674

End: 2297939

Strand: Reverse

Name: hisS

Synonym: Neut_2167

Alternate gene names: 114332132

Gene position: 2297939-2296674 (Counterclockwise)

Preceding gene: 114332133

Following gene: 114332131

Centisome position: 86.35

GC content: 49.21

Gene sequence:

>1266_bases
ATGCCTGAAGCAATCAGGGCAGTACGCGGGATGAACGACATCCTGCCTGATGAAAGTGATTTGTGGGGGTTTTTCGAAGA
TACGATCCGCACGTGGCTGGCTGCCTACGGTTATCGTAATTTACGAACCCCTATTGTTGAGCAGACTGATTTGTTCGTCC
GTTCAATTGGGGAAGTCACTGATATCGTCGAGAAAGAGATGTACTCGTTCATTGATCAACTCAATGGCGAAAATCTGACA
TTGCGTCCAGAAGGAACAGCTTCCTGTGTACGTGCAGTGATTGAGCATAATCTGCTGTATTCCGGACCCCAACGCCTGTA
CTACAGCGGCCCGATGTTTCGGCATGAACGCCCACAGAAGGGGCGTTATCGCCAGTTTCATCAGGTTGGTGTGGAAGCAC
TCGGATTTGTTGGCCCGGATATCGATGCAGAACTCATCATTATGTGCGCGCGGCTGTGGCGTTTGCTGGGTATTCCGGAT
GTGCGCCTTGAAATCAGCACGCTTGGCAGCATTGAATCACGTTTGATTTATCGTGCGCGCCTGATCAGCTATTTGGAAAA
ATTTTACGATGAGCTTGATGAAGATGCACGCCGGCGATTGAAAACCAACCCGTTGCGTATTCTTGACAGTAAAAATCCGG
GCATGCGTGAAATATTGGAAGGTGCACCCCATCTGTTCGATGATCTGGATGAAGATTCACTTATTCATTTTGACGCACTG
CAGCGGATTTTGCGTAACCAGGGGATCAGTTTTGAAATCAATCACCGTCTGGTCAGAGGGCTGGATTACTATAACCGCAC
GGTGTTCGAATGGGTGACTGACAAATTAGGGGCTCAGGGAACCATCTGTGCAGGTGGACGATATGACGGACTGATTGCGC
AGATTGGTGGCAAACCAGCTCCGGCATGCGGTTTTGCGATGGGAATAGAACGGATACTGTCTCTGATCAGTGAAACTGAT
GTGGCTGCTGAAATACACACTGTTCCTGATGTTTATGTTGTTCATCAAGGAGATATTGCAGCTGGGTTTTCCTGGAAAGT
CGCAGAATCCTTGCGGGATGCAGGCTTCAAGGTTGTGCTACATAGCGGCGGGGGAAGCTTCAAGGCACAGATGAAAAAAG
CTGATGCCAGTGGCGCACGGTTTGCCGCCATCATCGGGGACGATGAGGCTGCAACCGGACAGGTCAGTATCAAGTCATTA
CGTGAGGCAGTGGAGCAAGTCAAAATTGATTTGACGAAAGTCGCCGTGTTTCTGGAAAATATTTAA

Upstream 100 bases:

>100_bases
GGTCAAAAGACCGTTACCTTGAAAGGCGACAACATCGCTGGCGAATTCCGTGTAATCGTTGATGAATACGTGCGCACCAA
ATACCGGAAAAAAGTAGCTA

Downstream 100 bases:

>100_bases
TGAAGATGGAAGGTAAGAAATAATGTCTGCATACAACTTTGAAGAACAGGAAAAAATAGATCGGCTGAAGGCCTGGTGGT
CTGCTAATGGCGCAACGTTA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPEAIRAVRGMNDILPDESDLWGFFEDTIRTWLAAYGYRNLRTPIVEQTDLFVRSIGEVTDIVEKEMYSFIDQLNGENLT
LRPEGTASCVRAVIEHNLLYSGPQRLYYSGPMFRHERPQKGRYRQFHQVGVEALGFVGPDIDAELIIMCARLWRLLGIPD
VRLEISTLGSIESRLIYRARLISYLEKFYDELDEDARRRLKTNPLRILDSKNPGMREILEGAPHLFDDLDEDSLIHFDAL
QRILRNQGISFEINHRLVRGLDYYNRTVFEWVTDKLGAQGTICAGGRYDGLIAQIGGKPAPACGFAMGIERILSLISETD
VAAEIHTVPDVYVVHQGDIAAGFSWKVAESLRDAGFKVVLHSGGGSFKAQMKKADASGARFAAIIGDDEAATGQVSIKSL
REAVEQVKIDLTKVAVFLENI

Sequences:

>Translated_421_residues
MPEAIRAVRGMNDILPDESDLWGFFEDTIRTWLAAYGYRNLRTPIVEQTDLFVRSIGEVTDIVEKEMYSFIDQLNGENLT
LRPEGTASCVRAVIEHNLLYSGPQRLYYSGPMFRHERPQKGRYRQFHQVGVEALGFVGPDIDAELIIMCARLWRLLGIPD
VRLEISTLGSIESRLIYRARLISYLEKFYDELDEDARRRLKTNPLRILDSKNPGMREILEGAPHLFDDLDEDSLIHFDAL
QRILRNQGISFEINHRLVRGLDYYNRTVFEWVTDKLGAQGTICAGGRYDGLIAQIGGKPAPACGFAMGIERILSLISETD
VAAEIHTVPDVYVVHQGDIAAGFSWKVAESLRDAGFKVVLHSGGGSFKAQMKKADASGARFAAIIGDDEAATGQVSIKSL
REAVEQVKIDLTKVAVFLENI
>Mature_420_residues
PEAIRAVRGMNDILPDESDLWGFFEDTIRTWLAAYGYRNLRTPIVEQTDLFVRSIGEVTDIVEKEMYSFIDQLNGENLTL
RPEGTASCVRAVIEHNLLYSGPQRLYYSGPMFRHERPQKGRYRQFHQVGVEALGFVGPDIDAELIIMCARLWRLLGIPDV
RLEISTLGSIESRLIYRARLISYLEKFYDELDEDARRRLKTNPLRILDSKNPGMREILEGAPHLFDDLDEDSLIHFDALQ
RILRNQGISFEINHRLVRGLDYYNRTVFEWVTDKLGAQGTICAGGRYDGLIAQIGGKPAPACGFAMGIERILSLISETDV
AAEIHTVPDVYVVHQGDIAAGFSWKVAESLRDAGFKVVLHSGGGSFKAQMKKADASGARFAAIIGDDEAATGQVSIKSLR
EAVEQVKIDLTKVAVFLENI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=451, Percent_Identity=26.6075388026608, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=54.4811320754717, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71993693, Length=470, Percent_Identity=25.7446808510638, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71993686, Length=471, Percent_Identity=25.6900212314225, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_NITEC (Q0AE43)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748354.1
- ProteinModelPortal:   Q0AE43
- SMR:   Q0AE43
- STRING:   Q0AE43
- GeneID:   4272816
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2167
- NMPDR:   fig|335283.3.peg.218
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q0AE43
- ProtClustDB:   PRK00037
- BioCyc:   NEUT335283:NEUT_2167-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47239; Mature: 47107

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAIRAVRGMNDILPDESDLWGFFEDTIRTWLAAYGYRNLRTPIVEQTDLFVRSIGEVT
CCHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DIVEKEMYSFIDQLNGENLTLRPEGTASCVRAVIEHNLLYSGPQRLYYSGPMFRHERPQK
HHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
GRYRQFHQVGVEALGFVGPDIDAELIIMCARLWRLLGIPDVRLEISTLGSIESRLIYRAR
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
LISYLEKFYDELDEDARRRLKTNPLRILDSKNPGMREILEGAPHLFDDLDEDSLIHFDAL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCHHHHCCCCCCEEEHHHH
QRILRNQGISFEINHRLVRGLDYYNRTVFEWVTDKLGAQGTICAGGRYDGLIAQIGGKPA
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHEECCCCCC
PACGFAMGIERILSLISETDVAAEIHTVPDVYVVHQGDIAAGFSWKVAESLRDAGFKVVL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEE
HSGGGSFKAQMKKADASGARFAAIIGDDEAATGQVSIKSLREAVEQVKIDLTKVAVFLEN
ECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
I
C
>Mature Secondary Structure 
PEAIRAVRGMNDILPDESDLWGFFEDTIRTWLAAYGYRNLRTPIVEQTDLFVRSIGEVT
CHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DIVEKEMYSFIDQLNGENLTLRPEGTASCVRAVIEHNLLYSGPQRLYYSGPMFRHERPQK
HHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
GRYRQFHQVGVEALGFVGPDIDAELIIMCARLWRLLGIPDVRLEISTLGSIESRLIYRAR
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
LISYLEKFYDELDEDARRRLKTNPLRILDSKNPGMREILEGAPHLFDDLDEDSLIHFDAL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCHHHHCCCCCCEEEHHHH
QRILRNQGISFEINHRLVRGLDYYNRTVFEWVTDKLGAQGTICAGGRYDGLIAQIGGKPA
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHEECCCCCC
PACGFAMGIERILSLISETDVAAEIHTVPDVYVVHQGDIAAGFSWKVAESLRDAGFKVVL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEE
HSGGGSFKAQMKKADASGARFAAIIGDDEAATGQVSIKSLREAVEQVKIDLTKVAVFLEN
ECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA