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The map label for this gene is ispG [H]

Identifier: 114332133

GI number: 114332133

Start: 2297932

End: 2299176

Strand: Reverse

Name: ispG [H]

Synonym: Neut_2168

Alternate gene names: 114332133

Gene position: 2299176-2297932 (Counterclockwise)

Preceding gene: 114332134

Following gene: 114332132

Centisome position: 86.4

GC content: 52.69

Gene sequence:

>1245_bases
ATGTCTTCTAATAACGTACCTGCCCCGCGCCGAAAGTGTGTGGGTGTCAAAGTGGGTTCGGTGATGATTGGTGGTGGTGC
ACCGATTGTTGTGCAATCAATGACCAATACCGATACTGCTGATGAGCTGGCGACTGTCCAGCAGGTTGAACAGCTTGCGC
GTGCCGGTTCCGAACTTGTCAGAATTACCGTCAATTCCATGGAGGCAGCGCGAACAGTTCCCGCAATCCGCGCGCGGCTG
GATGATATGGATTGCCATGTTCCGCTGGTGGGTGATTTCCATTTCAATGGACACAAGTTGCTGACTGCTTATCCGGAATG
CGCAAAGGCGCTGGCCAAATACCGTATTAACCCGGGAAACGTGGGTCATGGGAAAAAGCGAGACGAACAGTTCTCCATAC
TGATTGAAATGGCCTGCAAATATGACAAACCAGTTCGTATCGGGGTGAATTGGGGTAGTCTCGATCCGGAGATGCTGGCG
CGGATCATGGATGAAAATGCGCGTTCTCCAGACCCGTTAGGTGCTTCACAGGTCATGCACAAGGCACTTATCACCTCTGC
GTTGGAAAGTGCGGCCAGGGCAGAAGAACTGGGCTTGGGTCGGGATCACATCGTGCTGTCATGCAAGGTTAGCGGTGTGC
AAGATCTGATTGCGGTTTATGGGGAGTTGGCTGCGCAGTGCGATTATGCACTGCATCTGGGGTTGACTGAAGCCGGCATG
GGGTCAAAGGGTATTGTTGCTTCGACAGCAGCACTGGCGGTGCTTTTGTTCAAGGGAATTGGCGATACGATTCGTATCTC
CCTGACGCCAGAGCCGGGCGGAGATCGCGCGCGTGAAGTTATCGTCGCTCAGGAAATCCTGCAGACAATGGGGTTGCGCG
CGTTTGTTCCGCTGGTGGCATCCTGTCCGGGATGCGGGCGCACAACCAGTACCTATTTCCAAGAACTTGCCGAAAGTATT
CAGATCTATGTGCGCGAGCAAATGATCATCTGGCGCGAACAATATGAAGGTGTCGAGAATATGTCGCTGGCAGTTATGGG
ATGTGTGGTCAACGGACCAGGTGAAAGCAAGCATGCCAATATTGGTATCAGTTTGCCCGGATCAGGTGAAACTCCGGTGG
CACCGGTTTTCGTGGATGGTCAAAAGACCGTTACCTTGAAAGGCGACAACATCGCTGGCGAATTCCGTGTAATCGTTGAT
GAATACGTGCGCACCAAATACCGGAAAAAAGTAGCTAATGCCTGA

Upstream 100 bases:

>100_bases
ACACAAGGTTGATCTTGCACCCTATACCCGCGGCAATGATGATGTCGCACGCTTTTCACTTGAATAACCTGTATAAATTT
CTGAGATCCGAGTGTTAACC

Downstream 100 bases:

>100_bases
AGCAATCAGGGCAGTACGCGGGATGAACGACATCCTGCCTGATGAAAGTGATTTGTGGGGGTTTTTCGAAGATACGATCC
GCACGTGGCTGGCTGCCTAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSSNNVPAPRRKCVGVKVGSVMIGGGAPIVVQSMTNTDTADELATVQQVEQLARAGSELVRITVNSMEAARTVPAIRARL
DDMDCHVPLVGDFHFNGHKLLTAYPECAKALAKYRINPGNVGHGKKRDEQFSILIEMACKYDKPVRIGVNWGSLDPEMLA
RIMDENARSPDPLGASQVMHKALITSALESAARAEELGLGRDHIVLSCKVSGVQDLIAVYGELAAQCDYALHLGLTEAGM
GSKGIVASTAALAVLLFKGIGDTIRISLTPEPGGDRAREVIVAQEILQTMGLRAFVPLVASCPGCGRTTSTYFQELAESI
QIYVREQMIIWREQYEGVENMSLAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGQKTVTLKGDNIAGEFRVIVD
EYVRTKYRKKVANA

Sequences:

>Translated_414_residues
MSSNNVPAPRRKCVGVKVGSVMIGGGAPIVVQSMTNTDTADELATVQQVEQLARAGSELVRITVNSMEAARTVPAIRARL
DDMDCHVPLVGDFHFNGHKLLTAYPECAKALAKYRINPGNVGHGKKRDEQFSILIEMACKYDKPVRIGVNWGSLDPEMLA
RIMDENARSPDPLGASQVMHKALITSALESAARAEELGLGRDHIVLSCKVSGVQDLIAVYGELAAQCDYALHLGLTEAGM
GSKGIVASTAALAVLLFKGIGDTIRISLTPEPGGDRAREVIVAQEILQTMGLRAFVPLVASCPGCGRTTSTYFQELAESI
QIYVREQMIIWREQYEGVENMSLAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGQKTVTLKGDNIAGEFRVIVD
EYVRTKYRKKVANA
>Mature_413_residues
SSNNVPAPRRKCVGVKVGSVMIGGGAPIVVQSMTNTDTADELATVQQVEQLARAGSELVRITVNSMEAARTVPAIRARLD
DMDCHVPLVGDFHFNGHKLLTAYPECAKALAKYRINPGNVGHGKKRDEQFSILIEMACKYDKPVRIGVNWGSLDPEMLAR
IMDENARSPDPLGASQVMHKALITSALESAARAEELGLGRDHIVLSCKVSGVQDLIAVYGELAAQCDYALHLGLTEAGMG
SKGIVASTAALAVLLFKGIGDTIRISLTPEPGGDRAREVIVAQEILQTMGLRAFVPLVASCPGCGRTTSTYFQELAESIQ
IYVREQMIIWREQYEGVENMSLAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGQKTVTLKGDNIAGEFRVIVDE
YVRTKYRKKVANA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=361, Percent_Identity=35.7340720221607, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44418; Mature: 44287

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNNVPAPRRKCVGVKVGSVMIGGGAPIVVQSMTNTDTADELATVQQVEQLARAGSELV
CCCCCCCCCHHHEEEEEECEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEE
RITVNSMEAARTVPAIRARLDDMDCHVPLVGDFHFNGHKLLTAYPECAKALAKYRINPGN
EEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEEEECHHHHHHHHHHCCCCCC
VGHGKKRDEQFSILIEMACKYDKPVRIGVNWGSLDPEMLARIMDENARSPDPLGASQVMH
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
KALITSALESAARAEELGLGRDHIVLSCKVSGVQDLIAVYGELAAQCDYALHLGLTEAGM
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCHHCCC
GSKGIVASTAALAVLLFKGIGDTIRISLTPEPGGDRAREVIVAQEILQTMGLRAFVPLVA
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
SCPGCGRTTSTYFQELAESIQIYVREQMIIWREQYEGVENMSLAVMGCVVNGPGESKHAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCE
IGISLPGSGETPVAPVFVDGQKTVTLKGDNIAGEFRVIVDEYVRTKYRKKVANA
EEEECCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSNNVPAPRRKCVGVKVGSVMIGGGAPIVVQSMTNTDTADELATVQQVEQLARAGSELV
CCCCCCCCHHHEEEEEECEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEE
RITVNSMEAARTVPAIRARLDDMDCHVPLVGDFHFNGHKLLTAYPECAKALAKYRINPGN
EEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEEEECHHHHHHHHHHCCCCCC
VGHGKKRDEQFSILIEMACKYDKPVRIGVNWGSLDPEMLARIMDENARSPDPLGASQVMH
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
KALITSALESAARAEELGLGRDHIVLSCKVSGVQDLIAVYGELAAQCDYALHLGLTEAGM
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCHHCCC
GSKGIVASTAALAVLLFKGIGDTIRISLTPEPGGDRAREVIVAQEILQTMGLRAFVPLVA
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHH
SCPGCGRTTSTYFQELAESIQIYVREQMIIWREQYEGVENMSLAVMGCVVNGPGESKHAN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCE
IGISLPGSGETPVAPVFVDGQKTVTLKGDNIAGEFRVIVDEYVRTKYRKKVANA
EEEECCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]