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The map label for this gene is glpK

Identifier: 114332046

GI number: 114332046

Start: 2201238

End: 2202737

Strand: Reverse

Name: glpK

Synonym: Neut_2080

Alternate gene names: 114332046

Gene position: 2202737-2201238 (Counterclockwise)

Preceding gene: 114332047

Following gene: 114332045

Centisome position: 82.78

GC content: 49.53

Gene sequence:

>1500_bases
ATGAATAACCAGCCAGTGATTCTGGCAATCGATCAGGGAACAACCAGCACTCGTGCCATTCTATTTTCGGCAACATTGGA
AATTCTCGCGGTGCAACAGAAAGAGCTGAAGCTACATTATCCACACAAGGGGTGGGTCGAACAGAATCCAGAAGCTATCT
GGCAGGATACGCTCGAAGTTTGTCATGCGGTACTGGAACACAATGTCAGTATGGTAGGGTCGGTTGCTGCTATCGGCATT
ACTAATCAGCGTGAAACAACTATTTTGTGGGATCGTAAAACCGGTAAACCTGTTTATCCTGCCATTGTCTGGCAGGATCG
TCGCACCGATATTGGTTGTGAGCAATTAAAAGCTCAAGGGTATGAGCCAATGGTAACAGCCCGGACTGGCTTGTTGTTTG
ATCCTTATTTTTCTGCAACTAAAATTGCCTGGATATTGGATAACGTCGAAGGAGTGCGTTCTCGGGCAATGCGTGGCGAG
CTGGCATTTGGAACAGTGGATTGTTATTTGCTCTGGCACCTGACCGGCGGGAAGGTGCATGCTACGGATGTGACGAATGC
TGCGCGGACACTGTTATTTAATATCGTTGAGCAAAAATGGGATGCAGATTTGCTGACATTGTTCAATATCCCGGAAACGA
TTCTGCCGGAAGTTCGCGATAACGCAGCCAGGTTTGGGATGACTGATCGGATCTTGTTCGGACGGGGAATTCCCATCGGT
GGTATGGCAGGGGATCAGCATGCGGCACTGATCGGGCAAAGATGCTTCCGGCCAGGTATGGTCAAGTCTACCTACGGTAC
AGGTTGTTTTGCTCTCATGAATATCGGCTCGGATTTTAAGCCTTCGCAGCATCGTTTGCTGACCACGCCAGCGTATCGTC
TGAACGGTAAGATGGTTTATGCTATCGAGGGGTCTATTTTTATTGCCGGAGCAGCGATTCAATGGTTGCGAGACGAGCTG
GAGTTTTTTCAGGAGGTTGCGGCGAGCGATGCATTGGCGCTCAGTGTGCCAGACAGCAATGAAGTGTATTTTGTGCCTGC
CTTTACTGGTCTCGGTGCGCCATATTGGCGGCCGGATGTACGAGGCATGATCAGCGGATTATCGCGTGATACAACGCGGG
TGCATATTGTACGTGCCGCACTGGAAGCGCAAGGCTACCAAACCCGGGATCTAATGGCGGCGATAGAAGAGGATGGCGGT
CATCATGCCGAGATTATCCGTGTAGATGGAGGGCTGGTAGCAAACAAATTCATGTGTCAGTTTCTTGCTGATATATTGAA
CAAGCCGGTGGAAGTGCCAAAAATAACGGAAGCAACAGCACTGGGTGCAGCGATCCTGGCCGGTCTGACGGTGGATTTGT
TTGCTGATCTGGAAGTAACTGGATGTTATTGGCAGCGGGATAAAATCTATACTCCTACGATAACAGAAACCGAAAGAGAA
CGATTGTATGCAGGCTGGAAAGCTGCAGTTCAATCGCTTCTGCACGCCTCCAGAAATTAG

Upstream 100 bases:

>100_bases
TTGTAGAAGTAACCTATCAGCCTGAGCGTTCCGGTGAATATTATCGGTAAGTGAACATGCTGTTTTCTGGAATTCTTTTG
TTCTTTGTATAAAACCAGTT

Downstream 100 bases:

>100_bases
CTTGTTGAGCTCATTAAAGTGAAGCCCGGTGTAAGTCGTGATTACTCTGGGTAGAGAGAACGTCTGTAGTAAATTATAAA
TATCTGCCTACTAATGTGAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNNQPVILAIDQGTTSTRAILFSATLEILAVQQKELKLHYPHKGWVEQNPEAIWQDTLEVCHAVLEHNVSMVGSVAAIGI
TNQRETTILWDRKTGKPVYPAIVWQDRRTDIGCEQLKAQGYEPMVTARTGLLFDPYFSATKIAWILDNVEGVRSRAMRGE
LAFGTVDCYLLWHLTGGKVHATDVTNAARTLLFNIVEQKWDADLLTLFNIPETILPEVRDNAARFGMTDRILFGRGIPIG
GMAGDQHAALIGQRCFRPGMVKSTYGTGCFALMNIGSDFKPSQHRLLTTPAYRLNGKMVYAIEGSIFIAGAAIQWLRDEL
EFFQEVAASDALALSVPDSNEVYFVPAFTGLGAPYWRPDVRGMISGLSRDTTRVHIVRAALEAQGYQTRDLMAAIEEDGG
HHAEIIRVDGGLVANKFMCQFLADILNKPVEVPKITEATALGAAILAGLTVDLFADLEVTGCYWQRDKIYTPTITETERE
RLYAGWKAAVQSLLHASRN

Sequences:

>Translated_499_residues
MNNQPVILAIDQGTTSTRAILFSATLEILAVQQKELKLHYPHKGWVEQNPEAIWQDTLEVCHAVLEHNVSMVGSVAAIGI
TNQRETTILWDRKTGKPVYPAIVWQDRRTDIGCEQLKAQGYEPMVTARTGLLFDPYFSATKIAWILDNVEGVRSRAMRGE
LAFGTVDCYLLWHLTGGKVHATDVTNAARTLLFNIVEQKWDADLLTLFNIPETILPEVRDNAARFGMTDRILFGRGIPIG
GMAGDQHAALIGQRCFRPGMVKSTYGTGCFALMNIGSDFKPSQHRLLTTPAYRLNGKMVYAIEGSIFIAGAAIQWLRDEL
EFFQEVAASDALALSVPDSNEVYFVPAFTGLGAPYWRPDVRGMISGLSRDTTRVHIVRAALEAQGYQTRDLMAAIEEDGG
HHAEIIRVDGGLVANKFMCQFLADILNKPVEVPKITEATALGAAILAGLTVDLFADLEVTGCYWQRDKIYTPTITETERE
RLYAGWKAAVQSLLHASRN
>Mature_499_residues
MNNQPVILAIDQGTTSTRAILFSATLEILAVQQKELKLHYPHKGWVEQNPEAIWQDTLEVCHAVLEHNVSMVGSVAAIGI
TNQRETTILWDRKTGKPVYPAIVWQDRRTDIGCEQLKAQGYEPMVTARTGLLFDPYFSATKIAWILDNVEGVRSRAMRGE
LAFGTVDCYLLWHLTGGKVHATDVTNAARTLLFNIVEQKWDADLLTLFNIPETILPEVRDNAARFGMTDRILFGRGIPIG
GMAGDQHAALIGQRCFRPGMVKSTYGTGCFALMNIGSDFKPSQHRLLTTPAYRLNGKMVYAIEGSIFIAGAAIQWLRDEL
EFFQEVAASDALALSVPDSNEVYFVPAFTGLGAPYWRPDVRGMISGLSRDTTRVHIVRAALEAQGYQTRDLMAAIEEDGG
HHAEIIRVDGGLVANKFMCQFLADILNKPVEVPKITEATALGAAILAGLTVDLFADLEVTGCYWQRDKIYTPTITETERE
RLYAGWKAAVQSLLHASRN

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=499, Percent_Identity=44.689378757515, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4504007, Length=499, Percent_Identity=44.689378757515, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI41393575, Length=499, Percent_Identity=44.8897795591182, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI42794763, Length=504, Percent_Identity=44.4444444444444, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=29.5049504950495, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=50.3018108651911, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1789987, Length=496, Percent_Identity=25.4032258064516, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=39.9602385685885, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=24.6653919694073, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71986147, Length=294, Percent_Identity=29.5918367346939, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=499, Percent_Identity=35.8717434869739, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=42.2396856581532, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655555, Length=503, Percent_Identity=39.9602385685885, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24655560, Length=503, Percent_Identity=39.9602385685885, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24655570, Length=503, Percent_Identity=39.9602385685885, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24655574, Length=503, Percent_Identity=39.9602385685885, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24655565, Length=503, Percent_Identity=39.9602385685885, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24654573, Length=438, Percent_Identity=42.9223744292237, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=33.965844402277, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=34.4827586206897, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24656367, Length=496, Percent_Identity=30.241935483871, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=29.1338582677165, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=32.1937321937322, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45552909, Length=311, Percent_Identity=30.5466237942122, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_NITEC (Q0AEC9)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748268.1
- ProteinModelPortal:   Q0AEC9
- SMR:   Q0AEC9
- STRING:   Q0AEC9
- GeneID:   4273194
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2080
- NMPDR:   fig|335283.3.peg.2211
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   GWVEHEP
- PhylomeDB:   Q0AEC9
- BioCyc:   NEUT335283:NEUT_2080-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55256; Mature: 55256

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 14-14 BINDING 18-18 BINDING 84-84 BINDING 136-136 BINDING 245-245 BINDING 267-267 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQPVILAIDQGTTSTRAILFSATLEILAVQQKELKLHYPHKGWVEQNPEAIWQDTLEV
CCCCCEEEEECCCCCCHHEEEHHHHHHHHEEECHHCEEECCCCCCCCCCCHHHHHHHHHH
CHAVLEHNVSMVGSVAAIGITNQRETTILWDRKTGKPVYPAIVWQDRRTDIGCEQLKAQG
HHHHHHCCHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCC
YEPMVTARTGLLFDPYFSATKIAWILDNVEGVRSRAMRGELAFGTVDCYLLWHLTGGKVH
CCCEEEECCCEEECCCCCHHEEEHHHHCHHHHHHHHHCCCEEEEEEEEEEEEEECCCEEE
ATDVTNAARTLLFNIVEQKWDADLLTLFNIPETILPEVRDNAARFGMTDRILFGRGIPIG
ECCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCHHHCCCCCEEEEECCCCCC
GMAGDQHAALIGQRCFRPGMVKSTYGTGCFALMNIGSDFKPSQHRLLTTPAYRLNGKMVY
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCEEECCEEEE
AIEGSIFIAGAAIQWLRDELEFFQEVAASDALALSVPDSNEVYFVPAFTGLGAPYWRPDV
EEECCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEHHCCCCCCCCCHH
RGMISGLSRDTTRVHIVRAALEAQGYQTRDLMAAIEEDGGHHAEIIRVDGGLVANKFMCQ
HHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHH
FLADILNKPVEVPKITEATALGAAILAGLTVDLFADLEVTGCYWQRDKIYTPTITETERE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEECEEEEEEEEECCEEECCCCCCCHHH
RLYAGWKAAVQSLLHASRN
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNQPVILAIDQGTTSTRAILFSATLEILAVQQKELKLHYPHKGWVEQNPEAIWQDTLEV
CCCCCEEEEECCCCCCHHEEEHHHHHHHHEEECHHCEEECCCCCCCCCCCHHHHHHHHHH
CHAVLEHNVSMVGSVAAIGITNQRETTILWDRKTGKPVYPAIVWQDRRTDIGCEQLKAQG
HHHHHHCCHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCC
YEPMVTARTGLLFDPYFSATKIAWILDNVEGVRSRAMRGELAFGTVDCYLLWHLTGGKVH
CCCEEEECCCEEECCCCCHHEEEHHHHCHHHHHHHHHCCCEEEEEEEEEEEEEECCCEEE
ATDVTNAARTLLFNIVEQKWDADLLTLFNIPETILPEVRDNAARFGMTDRILFGRGIPIG
ECCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCHHHCCCCCEEEEECCCCCC
GMAGDQHAALIGQRCFRPGMVKSTYGTGCFALMNIGSDFKPSQHRLLTTPAYRLNGKMVY
CCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCEEECCEEEE
AIEGSIFIAGAAIQWLRDELEFFQEVAASDALALSVPDSNEVYFVPAFTGLGAPYWRPDV
EEECCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEHHCCCCCCCCCHH
RGMISGLSRDTTRVHIVRAALEAQGYQTRDLMAAIEEDGGHHAEIIRVDGGLVANKFMCQ
HHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHH
FLADILNKPVEVPKITEATALGAAILAGLTVDLFADLEVTGCYWQRDKIYTPTITETERE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEECEEEEEEEEECCEEECCCCCCCHHH
RLYAGWKAAVQSLLHASRN
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA