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The map label for this gene is gcvH

Identifier: 114331927

GI number: 114331927

Start: 2068556

End: 2068945

Strand: Reverse

Name: gcvH

Synonym: Neut_1954

Alternate gene names: 114331927

Gene position: 2068945-2068556 (Counterclockwise)

Preceding gene: 114331928

Following gene: 114331926

Centisome position: 77.75

GC content: 45.38

Gene sequence:

>390_bases
ATGAGCGTTCCAGCAGAGCTGAAATATGCAAAATCACACGAATGGATCAAGTTGGAGGCAGATGGTACGGTAACAGTAGG
TATCACTCAACATGCCCAGGAATTACTGGGAGATATGGTGTTCGTTGAATTGCCCAAGGTAGGGCGCATCCTGGCGCAGC
AGGAAGATTGTGCGGTAGTGGAATCTGTCAAGGCGGCTTCAGATATTTATGCTCCACTCAGCGGAGAAGTTATTGCTATC
AATGCTGAGGTGGAATCATCCCCGGAAAAAATAAATGAAGATAGTTATTCAGCCTGGTTGTTTAAACTGAAGCCGGCCAA
TACGGCTGAAATTGATGGATTACTTGATGCCAACGGATATGAAAAGCTGCTGGAAAGTGATGCACATTAA

Upstream 100 bases:

>100_bases
TTTGTACGCAATGGCCAGGCACTGATCTGATTTTTGTAAAAGTATCAGCAGATAAAGCATTTTGAATTTTTAAACCTAAA
TTTAGGGGAGTGACGGATAA

Downstream 100 bases:

>100_bases
CAGGATGGTGAGAAACATTGATGAAGATGCCGTCGGAGCAAGGCAAAACAGGCAAAAAAGCGCAGTTTATGTATACGGTA
AATGAGCTTTGAGCCTGTTG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSVPAELKYAKSHEWIKLEADGTVTVGITQHAQELLGDMVFVELPKVGRILAQQEDCAVVESVKAASDIYAPLSGEVIAI
NAEVESSPEKINEDSYSAWLFKLKPANTAEIDGLLDANGYEKLLESDAH

Sequences:

>Translated_129_residues
MSVPAELKYAKSHEWIKLEADGTVTVGITQHAQELLGDMVFVELPKVGRILAQQEDCAVVESVKAASDIYAPLSGEVIAI
NAEVESSPEKINEDSYSAWLFKLKPANTAEIDGLLDANGYEKLLESDAH
>Mature_128_residues
SVPAELKYAKSHEWIKLEADGTVTVGITQHAQELLGDMVFVELPKVGRILAQQEDCAVVESVKAASDIYAPLSGEVIAIN
AEVESSPEKINEDSYSAWLFKLKPANTAEIDGLLDANGYEKLLESDAH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=44.1666666666667, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=43.3333333333333, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=61.6, Blast_Score=165, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=45.6896551724138, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=42.2413793103448, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=42.1875, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17865652, Length=122, Percent_Identity=43.4426229508197, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_NITEC (Q0AEP8)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748149.1
- ProteinModelPortal:   Q0AEP8
- SMR:   Q0AEP8
- STRING:   Q0AEP8
- GeneID:   4273306
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1954
- NMPDR:   fig|335283.3.peg.1077
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q0AEP8
- ProtClustDB:   PRK01202
- BioCyc:   NEUT335283:NEUT_1954-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14015; Mature: 13884

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPAELKYAKSHEWIKLEADGTVTVGITQHAQELLGDMVFVELPKVGRILAQQEDCAVV
CCCCCCCEECCCCCEEEEECCCEEEEEEHHHHHHHHHCEEEEECCCHHHHHHCCCCHHHH
ESVKAASDIYAPLSGEVIAINAEVESSPEKINEDSYSAWLFKLKPANTAEIDGLLDANGY
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCHH
EKLLESDAH
HHHHHCCCC
>Mature Secondary Structure 
SVPAELKYAKSHEWIKLEADGTVTVGITQHAQELLGDMVFVELPKVGRILAQQEDCAVV
CCCCCCEECCCCCEEEEECCCEEEEEEHHHHHHHHHCEEEEECCCHHHHHHCCCCHHHH
ESVKAASDIYAPLSGEVIAINAEVESSPEKINEDSYSAWLFKLKPANTAEIDGLLDANGY
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCHH
EKLLESDAH
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA