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The map label for this gene is metZ [H]

Identifier: 114331157

GI number: 114331157

Start: 1199131

End: 1200306

Strand: Direct

Name: metZ [H]

Synonym: Neut_1159

Alternate gene names: 114331157

Gene position: 1199131-1200306 (Clockwise)

Preceding gene: 114331156

Following gene: 114331158

Centisome position: 45.06

GC content: 51.19

Gene sequence:

>1176_bases
ATGACAAATGAATTAGATCTCCAAACGCTGGCTATTCATACCGGCGTCCATCGCAGTCAGTTTAACGAACATTCAGAATC
ACTCTATCTGACTTCAAGTTTTGTGTTTGACAGTGCAGCCCAGGCTGCTGCACGGTTCTCAGGGCAAGAACCCGGTAATA
TCTATTCGCGCTTTACTAACCCGACAGTAACTGCCATGCAGGAACGCCTAGCTGTGCTGGAAGGGGCGGAAGCCTGTATC
GCAACTGCTACTGGTATGGCAGCAATTACGACCTGCGTCATGGGACTACTTTCAGCTGGTGATCATATCGTTGCCTCACG
CAGCCTGTTTGGATCTACTGTTGGCTTGTTCAACAATATCCTCAGCCGTTTCAATATCGAAACTACCTTTGTTTCTGCAA
CCGATCCGAGTGAATGGCAGGCAGCAGCCAAACCAAACACCAAACTGTTTTTTATGGAAACTCCCTCCAATCCACTCACG
GAAATCTCCGATATCGCTGCATTGGCAGACATTGCCAAACGGATTGGGGTGTGGCTAGCGGTTGACAACAGTTTTTGTAC
CCCGGTTGTGCAACAACCACTGAAGCTGGGTGCAGATCTGGTCATTCACTCAGCAACAAAATATCTGGACGGGCAGGGCA
GAATCCTGGGAGGGGCAATACTGGGTAAACGTGATTTGCTGATGGATAGCGGTATATTCGGTTTCTTGCGAACAGCCGGC
CCGGCGCTAAGCGCATTCAATGCCTGGATTATTCTGAAAGGACTTGAAACATTAAGTGTACGGGTAAAAGCACACTCTGA
TCATGCACTGGAAGTCGCGCGCTGGCTGGAAGCACATCCTAATGTAGCACGTGTGTTTTACCCGGGTCTGCCGTCTCATC
CGCAGCATACACTTGCGATGCGCCAGCAGAAAACCGGTGGCAGCATTGTGTCATTCGAGGCAAAAGGCGGCAAAGAGGCT
GCCTGGCGTATTGTAGATGCTGCTCAGCTCATGTCGATTACTGCCAATCTTGGCGATACCAAAAGTACACTGACTCATCC
TGCCACTACAACGCATGGGCGTATCAGCCAGGAGGCGCGCGCAGCTGCCGGCGTCAGCGATGGTCTGTTACGTATTGCGG
TTGGCCTGGAGTCACCGGAAGACATCAAGGCAGATCTCGCTCGAGGATTGCAGTAG

Upstream 100 bases:

>100_bases
ATTGAATCACAGCGTAACAGTCTCGGGTTATCACAAGGGGAGCGCACTACCCAACTTGATTTAACCCTGCTCCCAATGGA
TTAACCATATAGAAACGATC

Downstream 100 bases:

>100_bases
ACTCGTAATTTATTACAACTGGCACACACTATTCTTCTTGAAACCGGATACGACTAGCAATAAATTTTGCTACCGGTTTT
CACTCACTACTACTGTGATA

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTNELDLQTLAIHTGVHRSQFNEHSESLYLTSSFVFDSAAQAAARFSGQEPGNIYSRFTNPTVTAMQERLAVLEGAEACI
ATATGMAAITTCVMGLLSAGDHIVASRSLFGSTVGLFNNILSRFNIETTFVSATDPSEWQAAAKPNTKLFFMETPSNPLT
EISDIAALADIAKRIGVWLAVDNSFCTPVVQQPLKLGADLVIHSATKYLDGQGRILGGAILGKRDLLMDSGIFGFLRTAG
PALSAFNAWIILKGLETLSVRVKAHSDHALEVARWLEAHPNVARVFYPGLPSHPQHTLAMRQQKTGGSIVSFEAKGGKEA
AWRIVDAAQLMSITANLGDTKSTLTHPATTTHGRISQEARAAAGVSDGLLRIAVGLESPEDIKADLARGLQ

Sequences:

>Translated_391_residues
MTNELDLQTLAIHTGVHRSQFNEHSESLYLTSSFVFDSAAQAAARFSGQEPGNIYSRFTNPTVTAMQERLAVLEGAEACI
ATATGMAAITTCVMGLLSAGDHIVASRSLFGSTVGLFNNILSRFNIETTFVSATDPSEWQAAAKPNTKLFFMETPSNPLT
EISDIAALADIAKRIGVWLAVDNSFCTPVVQQPLKLGADLVIHSATKYLDGQGRILGGAILGKRDLLMDSGIFGFLRTAG
PALSAFNAWIILKGLETLSVRVKAHSDHALEVARWLEAHPNVARVFYPGLPSHPQHTLAMRQQKTGGSIVSFEAKGGKEA
AWRIVDAAQLMSITANLGDTKSTLTHPATTTHGRISQEARAAAGVSDGLLRIAVGLESPEDIKADLARGLQ
>Mature_390_residues
TNELDLQTLAIHTGVHRSQFNEHSESLYLTSSFVFDSAAQAAARFSGQEPGNIYSRFTNPTVTAMQERLAVLEGAEACIA
TATGMAAITTCVMGLLSAGDHIVASRSLFGSTVGLFNNILSRFNIETTFVSATDPSEWQAAAKPNTKLFFMETPSNPLTE
ISDIAALADIAKRIGVWLAVDNSFCTPVVQQPLKLGADLVIHSATKYLDGQGRILGGAILGKRDLLMDSGIFGFLRTAGP
ALSAFNAWIILKGLETLSVRVKAHSDHALEVARWLEAHPNVARVFYPGLPSHPQHTLAMRQQKTGGSIVSFEAKGGKEAA
WRIVDAAQLMSITANLGDTKSTLTHPATTTHGRISQEARAAAGVSDGLLRIAVGLESPEDIKADLARGLQ

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=369, Percent_Identity=35.5013550135501, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI299473759, Length=369, Percent_Identity=31.9783197831978, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI25453487, Length=368, Percent_Identity=30.4347826086957, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=34.8958333333333, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789383, Length=367, Percent_Identity=27.2479564032698, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560056, Length=374, Percent_Identity=36.8983957219251, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17538055, Length=374, Percent_Identity=35.8288770053476, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=32.520325203252, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=34.7826086956522, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6323333, Length=439, Percent_Identity=32.5740318906606, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=29.2383292383292, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=273, Percent_Identity=27.8388278388278, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=406, Percent_Identity=24.384236453202, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=35.0515463917526, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 41736; Mature: 41605

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNELDLQTLAIHTGVHRSQFNEHSESLYLTSSFVFDSAAQAAARFSGQEPGNIYSRFTN
CCCCCCCEEEEEECCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
PTVTAMQERLAVLEGAEACIATATGMAAITTCVMGLLSAGDHIVASRSLFGSTVGLFNNI
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
LSRFNIETTFVSATDPSEWQAAAKPNTKLFFMETPSNPLTEISDIAALADIAKRIGVWLA
HHHCCCEEEEEECCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCEEEEE
VDNSFCTPVVQQPLKLGADLVIHSATKYLDGQGRILGGAILGKRDLLMDSGIFGFLRTAG
ECCCCCCHHHHHHHHCCCHHEEEHHHHHCCCCCCEEEEEEECCHHHHHHCCHHHHHHHCC
PALSAFNAWIILKGLETLSVRVKAHSDHALEVARWLEAHPNVARVFYPGLPSHPQHTLAM
HHHHHHHHEEEEECCEEEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
RQQKTGGSIVSFEAKGGKEAAWRIVDAAQLMSITANLGDTKSTLTHPATTTHGRISQEAR
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHH
AAAGVSDGLLRIAVGLESPEDIKADLARGLQ
HHCCCCCCEEEEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TNELDLQTLAIHTGVHRSQFNEHSESLYLTSSFVFDSAAQAAARFSGQEPGNIYSRFTN
CCCCCCEEEEEECCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
PTVTAMQERLAVLEGAEACIATATGMAAITTCVMGLLSAGDHIVASRSLFGSTVGLFNNI
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
LSRFNIETTFVSATDPSEWQAAAKPNTKLFFMETPSNPLTEISDIAALADIAKRIGVWLA
HHHCCCEEEEEECCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCEEEEE
VDNSFCTPVVQQPLKLGADLVIHSATKYLDGQGRILGGAILGKRDLLMDSGIFGFLRTAG
ECCCCCCHHHHHHHHCCCHHEEEHHHHHCCCCCCEEEEEEECCHHHHHHCCHHHHHHHCC
PALSAFNAWIILKGLETLSVRVKAHSDHALEVARWLEAHPNVARVFYPGLPSHPQHTLAM
HHHHHHHHEEEEECCEEEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
RQQKTGGSIVSFEAKGGKEAAWRIVDAAQLMSITANLGDTKSTLTHPATTTHGRISQEAR
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHH
AAAGVSDGLLRIAVGLESPEDIKADLARGLQ
HHCCCCCCEEEEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]