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The map label for this gene is purF [H]

Identifier: 114331156

GI number: 114331156

Start: 1197597

End: 1199114

Strand: Direct

Name: purF [H]

Synonym: Neut_1158

Alternate gene names: 114331156

Gene position: 1197597-1199114 (Clockwise)

Preceding gene: 114331155

Following gene: 114331157

Centisome position: 45.0

GC content: 48.42

Gene sequence:

>1518_bases
ATGTGTGGAATTCTAGGTGTAGTAGCCCGTTCCCCCGCCAATCAGTTGCTCTATGATGGCCTGTTAATGCTGCAACATCG
TGGCCAGGATGCTGCAGGTATTGTTACCGCGCAAGGTAATAGTTTTCATATGCACAAAAGGTTGGGTATGGTACGGGATG
TGTTCCGCACCCGGAATATGCGTGCTCTGCCAGGGTATATGGGAATCGGGCATGTTCGCTATCCCACGACAGGGTCATCA
AATTCTCCTGCGGAAGCACAGCCTTTTTATGTAAATTCCCCATTTGGTATTGTGCTGGCGCATAACGGCAACCTGACCAA
TGCGGATGTACTTAATCAAGAGCTGTTTCTTGCAGATCGCCGCCACGTAAACACGCATTCTGATTCGGAAGTGTTGCTGA
ATGTGCTGGCACATGAATTGCAGGAGCGCGCGACAGGTTACCAACTTGATCTGGCTGAAATTTTCTCGGCAGTTTCCGGT
GTACACGAGCGTTGTCAGGGTGCTTATGCAGTGATTGCAATGATTGCCGGTTATGGTTTGCTGGCTTTTCGCGATCCATG
CGGGATCAGGCCGCTGGTATTCGGATCAGTGGAAACGAATGCTGGTACTGAATACATGATTGCTTCTGAAAGTGTGGCGC
TGGATACACTGGGTTTTCGTCTGATCCGGGATGTTGCACCTGGAGAAGCTGTTTTTATTGATATGGAAGGGCATTTCTAC
AGCCATCAGTGTGCTGCTCACGCCAGTCTGAATCCCTGTATTTTTGAATATGTTTATCTGGCCCGTCCCGATTCAATGCT
GGATAGCATCTCGGTTTATGAGACTCGTCTCAATATGGGCGTAAACCTGGCAGATAAAATCAGCACCTCCATGCGGCACC
TGGATATTGATGTTGTAATACCGATTCCGGAATCCAGTCGTCCAAGTGCCATGCAGCTTGCCAATCGCCTCGGGATCAGC
TTCAGGGAAGGATTTGTCAAAAATCGCTATGTGGGCAGAACGTTTATTATGCCCGGGCAGCAGCAGCGCCGGAAATCGGT
TCGGCAGAAATTAAATGCCATCGAAATTGAATTTCGCGGCAAGAACGTACTGCTGGTTGATGATTCTATTGTGCGTGGCA
CCACTAGCCGAGAGATTGTTCAGATGGCACGAGAAGCGGGAGCACTTAAAATATACTTTGCTTCGGCAGCGCCACCAGTA
CGTTTCCCTAACGTATACGGTATAGATATGCCGACTCGCCAGGAATTGATTGCAACAGATCGTACCGATGAGGAAATATG
TCGTGAAATTGGTGCGGATTATCTGATCTATCAAAATCTGGATGCACTTAAGCAAGCGGTTGCCCAGGTCAATCCGCGCA
TCACGAATTTTGAAACCTCCTGTTTTGATGGGCATTACATAACAGGCAGTGTCACTCAGGAGTATCTTTACAGAATTGAA
TCACAGCGTAACAGTCTCGGGTTATCACAAGGGGAGCGCACTACCCAACTTGATTTAACCCTGCTCCCAATGGATTAA

Upstream 100 bases:

>100_bases
TTCAGGCTTGACTATATTGAAATTTCGTATCTTGTAATTAATACAGATATGAACTGCTAGTTTTACAGGTTTTTTAATCC
ACTTTTTCAGCGAGCTCGCT

Downstream 100 bases:

>100_bases
CCATATAGAAACGATCATGACAAATGAATTAGATCTCCAAACGCTGGCTATTCATACCGGCGTCCATCGCAGTCAGTTTA
ACGAACATTCAGAATCACTC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGILGVVARSPANQLLYDGLLMLQHRGQDAAGIVTAQGNSFHMHKRLGMVRDVFRTRNMRALPGYMGIGHVRYPTTGSS
NSPAEAQPFYVNSPFGIVLAHNGNLTNADVLNQELFLADRRHVNTHSDSEVLLNVLAHELQERATGYQLDLAEIFSAVSG
VHERCQGAYAVIAMIAGYGLLAFRDPCGIRPLVFGSVETNAGTEYMIASESVALDTLGFRLIRDVAPGEAVFIDMEGHFY
SHQCAAHASLNPCIFEYVYLARPDSMLDSISVYETRLNMGVNLADKISTSMRHLDIDVVIPIPESSRPSAMQLANRLGIS
FREGFVKNRYVGRTFIMPGQQQRRKSVRQKLNAIEIEFRGKNVLLVDDSIVRGTTSREIVQMAREAGALKIYFASAAPPV
RFPNVYGIDMPTRQELIATDRTDEEICREIGADYLIYQNLDALKQAVAQVNPRITNFETSCFDGHYITGSVTQEYLYRIE
SQRNSLGLSQGERTTQLDLTLLPMD

Sequences:

>Translated_505_residues
MCGILGVVARSPANQLLYDGLLMLQHRGQDAAGIVTAQGNSFHMHKRLGMVRDVFRTRNMRALPGYMGIGHVRYPTTGSS
NSPAEAQPFYVNSPFGIVLAHNGNLTNADVLNQELFLADRRHVNTHSDSEVLLNVLAHELQERATGYQLDLAEIFSAVSG
VHERCQGAYAVIAMIAGYGLLAFRDPCGIRPLVFGSVETNAGTEYMIASESVALDTLGFRLIRDVAPGEAVFIDMEGHFY
SHQCAAHASLNPCIFEYVYLARPDSMLDSISVYETRLNMGVNLADKISTSMRHLDIDVVIPIPESSRPSAMQLANRLGIS
FREGFVKNRYVGRTFIMPGQQQRRKSVRQKLNAIEIEFRGKNVLLVDDSIVRGTTSREIVQMAREAGALKIYFASAAPPV
RFPNVYGIDMPTRQELIATDRTDEEICREIGADYLIYQNLDALKQAVAQVNPRITNFETSCFDGHYITGSVTQEYLYRIE
SQRNSLGLSQGERTTQLDLTLLPMD
>Mature_505_residues
MCGILGVVARSPANQLLYDGLLMLQHRGQDAAGIVTAQGNSFHMHKRLGMVRDVFRTRNMRALPGYMGIGHVRYPTTGSS
NSPAEAQPFYVNSPFGIVLAHNGNLTNADVLNQELFLADRRHVNTHSDSEVLLNVLAHELQERATGYQLDLAEIFSAVSG
VHERCQGAYAVIAMIAGYGLLAFRDPCGIRPLVFGSVETNAGTEYMIASESVALDTLGFRLIRDVAPGEAVFIDMEGHFY
SHQCAAHASLNPCIFEYVYLARPDSMLDSISVYETRLNMGVNLADKISTSMRHLDIDVVIPIPESSRPSAMQLANRLGIS
FREGFVKNRYVGRTFIMPGQQQRRKSVRQKLNAIEIEFRGKNVLLVDDSIVRGTTSREIVQMAREAGALKIYFASAAPPV
RFPNVYGIDMPTRQELIATDRTDEEICREIGADYLIYQNLDALKQAVAQVNPRITNFETSCFDGHYITGSVTQEYLYRIE
SQRNSLGLSQGERTTQLDLTLLPMD

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=35.5042016806723, Blast_Score=269, Evalue=5e-72,
Organism=Escherichia coli, GI1788651, Length=487, Percent_Identity=60.3696098562628, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI1790167, Length=226, Percent_Identity=31.4159292035398, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=34.9272349272349, Blast_Score=252, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=509, Percent_Identity=50.6876227897839, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24659598, Length=494, Percent_Identity=35.6275303643725, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=36.3829787234043, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI28573187, Length=496, Percent_Identity=35.2822580645161, Blast_Score=264, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56093; Mature: 56093

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGILGVVARSPANQLLYDGLLMLQHRGQDAAGIVTAQGNSFHMHKRLGMVRDVFRTRNM
CCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCC
RALPGYMGIGHVRYPTTGSSNSPAEAQPFYVNSPFGIVLAHNGNLTNADVLNQELFLADR
EECCCCCCCCCEECCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHCC
RHVNTHSDSEVLLNVLAHELQERATGYQLDLAEIFSAVSGVHERCQGAYAVIAMIAGYGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFRDPCGIRPLVFGSVETNAGTEYMIASESVALDTLGFRLIRDVAPGEAVFIDMEGHFY
EEECCCCCCCCEEEECEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCEE
SHQCAAHASLNPCIFEYVYLARPDSMLDSISVYETRLNMGVNLADKISTSMRHLDIDVVI
CCHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEE
PIPESSRPSAMQLANRLGISFREGFVKNRYVGRTFIMPGQQQRRKSVRQKLNAIEIEFRG
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCEEEEEECC
KNVLLVDDSIVRGTTSREIVQMAREAGALKIYFASAAPPVRFPNVYGIDMPTRQELIATD
CEEEEECCHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
RTDEEICREIGADYLIYQNLDALKQAVAQVNPRITNFETSCFDGHYITGSVTQEYLYRIE
CCHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHH
SQRNSLGLSQGERTTQLDLTLLPMD
HHHCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MCGILGVVARSPANQLLYDGLLMLQHRGQDAAGIVTAQGNSFHMHKRLGMVRDVFRTRNM
CCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCC
RALPGYMGIGHVRYPTTGSSNSPAEAQPFYVNSPFGIVLAHNGNLTNADVLNQELFLADR
EECCCCCCCCCEECCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHCC
RHVNTHSDSEVLLNVLAHELQERATGYQLDLAEIFSAVSGVHERCQGAYAVIAMIAGYGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFRDPCGIRPLVFGSVETNAGTEYMIASESVALDTLGFRLIRDVAPGEAVFIDMEGHFY
EEECCCCCCCCEEEECEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCEE
SHQCAAHASLNPCIFEYVYLARPDSMLDSISVYETRLNMGVNLADKISTSMRHLDIDVVI
CCHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEE
PIPESSRPSAMQLANRLGISFREGFVKNRYVGRTFIMPGQQQRRKSVRQKLNAIEIEFRG
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCEEEEEECC
KNVLLVDDSIVRGTTSREIVQMAREAGALKIYFASAAPPVRFPNVYGIDMPTRQELIATD
CEEEEECCHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
RTDEEICREIGADYLIYQNLDALKQAVAQVNPRITNFETSCFDGHYITGSVTQEYLYRIE
CCHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHH
SQRNSLGLSQGERTTQLDLTLLPMD
HHHCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]