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The map label for this gene is nuoB

Identifier: 114330926

GI number: 114330926

Start: 949077

End: 949553

Strand: Direct

Name: nuoB

Synonym: Neut_0923

Alternate gene names: 114330926

Gene position: 949077-949553 (Clockwise)

Preceding gene: 114330925

Following gene: 114330927

Centisome position: 35.67

GC content: 46.75

Gene sequence:

>477_bases
ATGGGTATTGAGGGTGCATTAGACAAAGGATTTGTTACCACAAACCTTGATTCCTTGATTAATTGGGGACGCACAGGATC
AATGTGGCCTATGACATTTGGGCTGGCTTGTTGCGCAGTTGAGATGATGCAAACCGGTGCATCTCGCTATGACTTGGATC
GGTTTGGAATTGTTTTTCGTCCGAGTCCCCGTCAGTCAGATGTCATGATCGTTGCGGGTACGCTATGTAATAAAATGGCA
CCAGCTTTGCGCAAGGTTTACGATCAAATGGCCGAACCTCGTTGGGTAATTTCCATGGGATCCTGTGCAAATGGTGGTGG
GTATTATCATTATTCCTATTCGGTTGTCCGAGGGTGCGATCGGATAGTGCCTGTCGATATTTATGTGCCGGGTTGTCCTC
CTACTGCAGAAGCTTTGCTTTATGGGATTATTCAGCTGCAGAATAAAATCTGGCGTACTAACACAATCGCGCGTTAG

Upstream 100 bases:

>100_bases
GGGATTGTTTGGTTTTATTGCGATGCTAGTATTTCTTGGATTGCTCGTCGTCGGCTTTATTTACGAGTGGGCTAAGGGTG
CTTTGGAATGGGATTAAATA

Downstream 100 bases:

>100_bases
AGAATAAAAATGACAGATAGTCGCGTAGATAAACTTGCAACAAATTTACAGCAAGTACTGAGTGATCAGCAGGTTGATAT
GAGTCATGCGTTAGGTGAGC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MGIEGALDKGFVTTNLDSLINWGRTGSMWPMTFGLACCAVEMMQTGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIWRTNTIAR

Sequences:

>Translated_158_residues
MGIEGALDKGFVTTNLDSLINWGRTGSMWPMTFGLACCAVEMMQTGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIWRTNTIAR
>Mature_157_residues
GIEGALDKGFVTTNLDSLINWGRTGSMWPMTFGLACCAVEMMQTGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIWRTNTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=80.1369863013699, Blast_Score=257, Evalue=3e-69,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=51.006711409396, Blast_Score=169, Evalue=7e-44,
Organism=Escherichia coli, GI1789074, Length=107, Percent_Identity=44.8598130841121, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=38.5245901639344, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=71.1267605633803, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=80.8510638297872, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=80.8510638297872, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=78.0821917808219, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_NITEC (Q0AHJ9)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747148.1
- ProteinModelPortal:   Q0AHJ9
- SMR:   Q0AHJ9
- STRING:   Q0AHJ9
- GeneID:   4272802
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0923
- NMPDR:   fig|335283.3.peg.1581
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q0AHJ9
- ProtClustDB:   PRK06411
- BioCyc:   NEUT335283:NEUT_0923-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17487; Mature: 17355

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIEGALDKGFVTTNLDSLINWGRTGSMWPMTFGLACCAVEMMQTGASRYDLDRFGIVFR
CCCCCCCCCCEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALLYGIIQLQNKIWRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GIEGALDKGFVTTNLDSLINWGRTGSMWPMTFGLACCAVEMMQTGASRYDLDRFGIVFR
CCCCCCCCCEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALLYGIIQLQNKIWRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA