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The map label for this gene is hemK [H]

Identifier: 114330835

GI number: 114330835

Start: 844715

End: 845596

Strand: Direct

Name: hemK [H]

Synonym: Neut_0828

Alternate gene names: 114330835

Gene position: 844715-845596 (Clockwise)

Preceding gene: 114330834

Following gene: 114330836

Centisome position: 31.74

GC content: 46.6

Gene sequence:

>882_bases
ATGATAGGAAATATGCTTTCCTCGGAACAACAGCAAGGTATAAACACGCCCGCTACAATTGGTGAACTCTTGCAGAATGC
AGCAAGTGTTGTAGATCGGATTGATGCGCGTTGGCTATTGCAAAGTGTACTGAACGTGGATGCGGCTTTTCTGATTACAC
ATGCAGAATTGTTACTCGGTATGGAGCAGATTGTACATTTCCAGCAATTGCTTGCGAGGCGCATGGCGGGGGAACCTGTT
GCATATCTGACGGGCGAAAGAGGGTTTTACGATCTGGTTTTTGACGTGACGCCAGATGTTTTGATTCCTCGCCCCGAAAC
GGAACTATTGGTGGAAATGGCATTGTCTAAAATTCCACCAGATCGGTGCTGTAACATTCTTGATTTGGGCACCGGAAGCG
GTGCTATTGCCATTACAATCGCCAGACATCGTCCTGATATTTATGTGACAGCAGTTGATCTTTCTCCTCTGGCACTGGCA
GTGGCGCGCAGAAATGCTAAAAGATGTTCTGTAGAAAATGTGGTATTTATTAAAGCAGACTGGTTTTCGGGTTTTATCAG
TGAAAAGTTTGATGTGATTGTGGCTAATCCGCCTTATATAGTCGAGGGAGATCCGCATCTTGAAGCCGATGGCCTGCGTT
TTGAACCCACTATCGCACTGGTGGCCCAGAATAACGGCCTGGATTGTATTCGTAGAATAGTTGATCAGGCTCCGGATTAT
CTGGAACATTCCGGATGGTTAATGCTTGAACATGGATACGACCAGGCTGATGTCTGCCGCAGATTGCTGGATAAAACCGG
TTTTTCCCATATATTCACCAGATCAGATCTTGCTGGGATAGATAGGGTAACGGGGGGACAGTATGGATTGGTTCCGGCAT
AG

Upstream 100 bases:

>100_bases
TCTATAAAATAGAGCAGATTATGGACGGTGAGTTAGATGAATTATGCTCTACTTTGGCAGCCGAACATCAGGCCGCACAG
CTGGCTGCCATGATCGAAAG

Downstream 100 bases:

>100_bases
AAGTTTATATCACCATCTGGAAATTATTCTGGATATAAAGATTCAAACAGGATGAGTAGTATTAACCAAGGTTTTGTATA
AATTTGTTCCAGTTGAAGTG

Product: modification methylase, HemK family protein

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MIGNMLSSEQQQGINTPATIGELLQNAASVVDRIDARWLLQSVLNVDAAFLITHAELLLGMEQIVHFQQLLARRMAGEPV
AYLTGERGFYDLVFDVTPDVLIPRPETELLVEMALSKIPPDRCCNILDLGTGSGAIAITIARHRPDIYVTAVDLSPLALA
VARRNAKRCSVENVVFIKADWFSGFISEKFDVIVANPPYIVEGDPHLEADGLRFEPTIALVAQNNGLDCIRRIVDQAPDY
LEHSGWLMLEHGYDQADVCRRLLDKTGFSHIFTRSDLAGIDRVTGGQYGLVPA

Sequences:

>Translated_293_residues
MIGNMLSSEQQQGINTPATIGELLQNAASVVDRIDARWLLQSVLNVDAAFLITHAELLLGMEQIVHFQQLLARRMAGEPV
AYLTGERGFYDLVFDVTPDVLIPRPETELLVEMALSKIPPDRCCNILDLGTGSGAIAITIARHRPDIYVTAVDLSPLALA
VARRNAKRCSVENVVFIKADWFSGFISEKFDVIVANPPYIVEGDPHLEADGLRFEPTIALVAQNNGLDCIRRIVDQAPDY
LEHSGWLMLEHGYDQADVCRRLLDKTGFSHIFTRSDLAGIDRVTGGQYGLVPA
>Mature_293_residues
MIGNMLSSEQQQGINTPATIGELLQNAASVVDRIDARWLLQSVLNVDAAFLITHAELLLGMEQIVHFQQLLARRMAGEPV
AYLTGERGFYDLVFDVTPDVLIPRPETELLVEMALSKIPPDRCCNILDLGTGSGAIAITIARHRPDIYVTAVDLSPLALA
VARRNAKRCSVENVVFIKADWFSGFISEKFDVIVANPPYIVEGDPHLEADGLRFEPTIALVAQNNGLDCIRRIVDQAPDY
LEHSGWLMLEHGYDQADVCRRLLDKTGFSHIFTRSDLAGIDRVTGGQYGLVPA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=207, Percent_Identity=32.8502415458937, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1787463, Length=264, Percent_Identity=43.5606060606061, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI87082085, Length=175, Percent_Identity=39.4285714285714, Blast_Score=107, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24582226, Length=281, Percent_Identity=27.7580071174377, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32293; Mature: 32293

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNMLSSEQQQGINTPATIGELLQNAASVVDRIDARWLLQSVLNVDAAFLITHAELLLG
CCCCHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MEQIVHFQQLLARRMAGEPVAYLTGERGFYDLVFDVTPDVLIPRPETELLVEMALSKIPP
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHCCH
DRCCNILDLGTGSGAIAITIARHRPDIYVTAVDLSPLALAVARRNAKRCSVENVVFIKAD
HHCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEEH
WFSGFISEKFDVIVANPPYIVEGDPHLEADGLRFEPTIALVAQNNGLDCIRRIVDQAPDY
HHHHHHCCCCEEEECCCCEEECCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHHCCHH
LEHSGWLMLEHGYDQADVCRRLLDKTGFSHIFTRSDLAGIDRVTGGQYGLVPA
HHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHCCCHHCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MIGNMLSSEQQQGINTPATIGELLQNAASVVDRIDARWLLQSVLNVDAAFLITHAELLLG
CCCCHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MEQIVHFQQLLARRMAGEPVAYLTGERGFYDLVFDVTPDVLIPRPETELLVEMALSKIPP
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHCCH
DRCCNILDLGTGSGAIAITIARHRPDIYVTAVDLSPLALAVARRNAKRCSVENVVFIKAD
HHCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEEH
WFSGFISEKFDVIVANPPYIVEGDPHLEADGLRFEPTIALVAQNNGLDCIRRIVDQAPDY
HHHHHHCCCCEEEECCCCEEECCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHHCCHH
LEHSGWLMLEHGYDQADVCRRLLDKTGFSHIFTRSDLAGIDRVTGGQYGLVPA
HHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHCCCHHCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]