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The map label for this gene is prfA

Identifier: 114330834

GI number: 114330834

Start: 843639

End: 844718

Strand: Direct

Name: prfA

Synonym: Neut_0827

Alternate gene names: 114330834

Gene position: 843639-844718 (Clockwise)

Preceding gene: 114330833

Following gene: 114330835

Centisome position: 31.7

GC content: 48.8

Gene sequence:

>1080_bases
ATGAATAAGGGAATTGTGGATCAGCTTACCCGGCTGAGTATACGACTGGAGGAATTGGACAAGCTGTTGAGCAATGAAAA
GATCACGGCTGATCTGGATAATTATCGGAAACTTTCTCGTGAACGATCTGAAATTGAGCCGGTAACCAGGTTTTATCATA
CTTACCAGCAGGTTGAGCAAGATTTGGCTACCGCTATGGAAATGAGTGTGGATCCGCAATTCCGGGATTTTGCTGAAGCC
GAGATTGATGCAGATAAGCAGAAGCTGGCGGATATTGAAACCGAGATACTCAAGCAGCTGTTGCCCAGGGATCCGAATGA
TGAGCGCAACATTTTCCTGGAAATACGGGCCGGTACGGGAGGGGATGAATCTGCTCTGTTTGCAGGTGACTTGTTTCGCA
TGTATAGCCGCTATGCGGAGCGTGAGGGATGGCAAGTAGAAATTGTTTCCCAGAATCCTTCTGAAGTGGGCGGATACAAG
GAGATTATTGCCCGGATTATTGGCCACGGTGCCTATTCTAGACTTAAATTTGAATCGGGTGCACATCGGGTGCAGCGTGT
TCCGGCGACTGAAACACAGGGGCGTGTGCATACTTCAACCTGTACTGTTGCTGTGCTTCCGGAAGCAGATGAAATTTCTG
GCGTAACTCTGAATCCGGCTGATTTAAGAATCGATACATTCCGCGCTTCCGGCGCGGGCGGGCAGCATATTAATAAAACA
GATTCGGCTGTACGGATTACCCATTTGCCGACAGGAATTGTGGCTGAGTGTCAGGAAGGGCGTTCCCAGCATAAGAATAA
GGCACAGGCAATGAGTGTGTTGATTTCCCGTATTCTCGACAAGCAGGTGCGTGCGCAACAGGCTGAACAGGCAGCTGCCA
GGAAATCGCTGGTTGGCAGCGGTGAACGTTCAGAGCGGATCCGGACCTATAATTTTCCACAAGGGAGAATTACTGATCAC
AGAATTAACCTGACACTCTATAAAATAGAGCAGATTATGGACGGTGAGTTAGATGAATTATGCTCTACTTTGGCAGCCGA
ACATCAGGCCGCACAGCTGGCTGCCATGATCGAAAGATGA

Upstream 100 bases:

>100_bases
TTCTCCATTCTCCTTCTTCCGCTCTCAACCAGGTATCCAATGATGAGCGTGAGCAACTTGTTGAGCTTGTCAATCGGTTG
TATCAGTTGCGTTATCCACG

Downstream 100 bases:

>100_bases
TAGGAAATATGCTTTCCTCGGAACAACAGCAAGGTATAAACACGCCCGCTACAATTGGTGAACTCTTGCAGAATGCAGCA
AGTGTTGTAGATCGGATTGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNKGIVDQLTRLSIRLEELDKLLSNEKITADLDNYRKLSRERSEIEPVTRFYHTYQQVEQDLATAMEMSVDPQFRDFAEA
EIDADKQKLADIETEILKQLLPRDPNDERNIFLEIRAGTGGDESALFAGDLFRMYSRYAEREGWQVEIVSQNPSEVGGYK
EIIARIIGHGAYSRLKFESGAHRVQRVPATETQGRVHTSTCTVAVLPEADEISGVTLNPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVAECQEGRSQHKNKAQAMSVLISRILDKQVRAQQAEQAAARKSLVGSGERSERIRTYNFPQGRITDH
RINLTLYKIEQIMDGELDELCSTLAAEHQAAQLAAMIER

Sequences:

>Translated_359_residues
MNKGIVDQLTRLSIRLEELDKLLSNEKITADLDNYRKLSRERSEIEPVTRFYHTYQQVEQDLATAMEMSVDPQFRDFAEA
EIDADKQKLADIETEILKQLLPRDPNDERNIFLEIRAGTGGDESALFAGDLFRMYSRYAEREGWQVEIVSQNPSEVGGYK
EIIARIIGHGAYSRLKFESGAHRVQRVPATETQGRVHTSTCTVAVLPEADEISGVTLNPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVAECQEGRSQHKNKAQAMSVLISRILDKQVRAQQAEQAAARKSLVGSGERSERIRTYNFPQGRITDH
RINLTLYKIEQIMDGELDELCSTLAAEHQAAQLAAMIER
>Mature_359_residues
MNKGIVDQLTRLSIRLEELDKLLSNEKITADLDNYRKLSRERSEIEPVTRFYHTYQQVEQDLATAMEMSVDPQFRDFAEA
EIDADKQKLADIETEILKQLLPRDPNDERNIFLEIRAGTGGDESALFAGDLFRMYSRYAEREGWQVEIVSQNPSEVGGYK
EIIARIIGHGAYSRLKFESGAHRVQRVPATETQGRVHTSTCTVAVLPEADEISGVTLNPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVAECQEGRSQHKNKAQAMSVLISRILDKQVRAQQAEQAAARKSLVGSGERSERIRTYNFPQGRITDH
RINLTLYKIEQIMDGELDELCSTLAAEHQAAQLAAMIER

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=273, Percent_Identity=51.2820512820513, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI34577120, Length=356, Percent_Identity=39.6067415730337, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=52.247191011236, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=58.7743732590529, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=38.368580060423, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=295, Percent_Identity=34.9152542372881, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=354, Percent_Identity=41.2429378531073, Blast_Score=242, Evalue=5e-65,
Organism=Drosophila melanogaster, GI19921226, Length=301, Percent_Identity=41.1960132890365, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_NITEC (Q0AHU1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747056.1
- ProteinModelPortal:   Q0AHU1
- SMR:   Q0AHU1
- STRING:   Q0AHU1
- GeneID:   4272897
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0827
- NMPDR:   fig|335283.3.peg.2540
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q0AHU1
- ProtClustDB:   PRK00591
- BioCyc:   NEUT335283:NEUT_0827-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40336; Mature: 40336

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGIVDQLTRLSIRLEELDKLLSNEKITADLDNYRKLSRERSEIEPVTRFYHTYQQVEQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAMEMSVDPQFRDFAEAEIDADKQKLADIETEILKQLLPRDPNDERNIFLEIRAGTG
HHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLFRMYSRYAEREGWQVEIVSQNPSEVGGYKEIIARIIGHGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHCCCHHHHHEEHHH
AHRVQRVPATETQGRVHTSTCTVAVLPEADEISGVTLNPADLRIDTFRASGAGGQHINKT
HHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCEECCHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVAECQEGRSQHKNKAQAMSVLISRILDKQVRAQQAEQAAARKSLVGS
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GERSERIRTYNFPQGRITDHRINLTLYKIEQIMDGELDELCSTLAAEHQAAQLAAMIER
CCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKGIVDQLTRLSIRLEELDKLLSNEKITADLDNYRKLSRERSEIEPVTRFYHTYQQVEQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAMEMSVDPQFRDFAEAEIDADKQKLADIETEILKQLLPRDPNDERNIFLEIRAGTG
HHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLFRMYSRYAEREGWQVEIVSQNPSEVGGYKEIIARIIGHGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHCCCHHHHHEEHHH
AHRVQRVPATETQGRVHTSTCTVAVLPEADEISGVTLNPADLRIDTFRASGAGGQHINKT
HHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCEECCHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVAECQEGRSQHKNKAQAMSVLISRILDKQVRAQQAEQAAARKSLVGS
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GERSERIRTYNFPQGRITDHRINLTLYKIEQIMDGELDELCSTLAAEHQAAQLAAMIER
CCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA