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The map label for this gene is clpB [H]

Identifier: 114330749

GI number: 114330749

Start: 749885

End: 752476

Strand: Reverse

Name: clpB [H]

Synonym: Neut_0734

Alternate gene names: 114330749

Gene position: 752476-749885 (Counterclockwise)

Preceding gene: 114330750

Following gene: 114330748

Centisome position: 28.28

GC content: 50.93

Gene sequence:

>2592_bases
ATGCGGTTCGACAAATTCACCACCAAGTTTCAGCAGGCTCTCGCCGATGCGCAAAGCATGGCGCTGGGGCAGGATCATCC
CTATATTGAATCTCAGCATCTTCTGCTCGCACTGATGCAGCAAGAAGATGGCAGTATTGCCTCGTTGCTGCAACGTGCCG
GAGTGAATGTTCAACCATTGCGCCAGGCATTGACACAAAGCCTGAAACTTTTGCCAAAAGTGGAAGGAACAGGCGGGGAG
ATCAATATTTCTCGTGATCTGGGCAATCTGCTCAACCTGACTGACAAGGAGGCGCAGAAAAGAGGAGATCAGTACATTGC
TTCAGAAATGTTTCTGCTGGCTGTACTGGAAGACAAAGGCGAGACCGGACGATTGCTGAAACAATACGGTGTTACACGCG
CTGCACTGGAACAGACAATCGATTCTGTACGCGGCGGAGAACAGGTAACCGATGCTGAAGCAGAAGGCAGTCGGGAAGCC
CTGAAGAAATATACGCTGGATCTGACTGAACGTGCCCGCTCGGGCAAGCTCGATCCCGTGATCGGACGGGATGACGAAAT
CCGGCGGACAATTCAGGTATTGCAACGGCGTACCAAGAACAACCCCGTCCTGATTGGTGAGCCGGGCGTCGGTAAAACAG
CCATTGTGGAAGGGTTGGCGCAACGTATCGTCAATGGTGAAGTGCCCGAAACACTGAAAAACAAACGAGTGTTATCGCTG
GACATGGCAGCACTGCTGGCCGGCGCCAAATATCGTGGCGAGTTTGAAGAGCGATTGAAGACTGTACTGAAAGAGCTGGC
GCAAGATGAAGGCCGTACTATCGTGTTCATTGATGAACTGCATACGATGGTAGGTGCTGGTAAGGCAGAAGGCGCGATAG
ATGCCGGCAACATGCTCAAACCAGCACTGGCACGCGGTGAGCTGCATTGCGTGGGTGCAACCACGCTGGATGAATACCGC
AAATACGTTGAAAAGGATGCTGCGTTGGAACGCCGTTTCCAGAAAGTACTGGTGGACGAACCCGGCGTCGAGGCGACGAT
CGCCATCCTGCGCGGGCTGCAGGAAAAATACGAATTGCATCATGGTGTGGAAATTACTGATCCAGCTATTGTTGCGGCTG
CCGAACTCTCCCATCGTTACATTACCGACCGTTTTCTGCCAGACAAGGCAATCGATCTGATCGACGAGGCGGCAGCACGC
ATTCGCATGGAGCAGGATTCCAAGCCGGAAGTCATGGATAAACTTGATCGGCGCCTTATTCAGCTGAAGATCGAGCGTGA
AGCCGTAAAAAAGGAAAAGGATGAGGCCTCGAAAAAACGTCTGGCACTGCTTGAAGAGGAAATTGAAAAACTGGAACGTG
AATACGCTGATCTGGACGAAATACTGAAAGCGGAAAAGTCCCGCGCGAAAGGTTCACAGGAAATTAAGGAAGAGCTTGAG
AAACTGCGTCGGGAAGAAGAAGCCGCACGGCGTAAGGGGGACTTGCAAAAAGCATCAGAGTTACTATACGGCCGTATTCC
GCAATTGGAAACACAGCTTGCAGAGCAATTGCACCACGATGAATCAGCCGAGGAGGCTACACAGCCAAAATTATTCCGAA
CCCAGGTGGGCGCTGAGGAAATTGCTGAGGTTGTTTCGCGTGCAACCGGTATCCCGGTTTCCAAAATGATGCAGGGCGAG
CGCGAAAAACTGTTGTTCATGGAAGATAAATTGCATGAACGGGTCATTGGTCAGGATGAGGCTGTCCGGCTGGTTTCTGA
CGCAATCCGGCGCTCTCGTTCCGGGTTATCCGACCCCAACCGACCCTATGGATCTTTCTTGTTTCTGGGACCGACTGGGG
TAGGTAAAACCGAGTTGTGCAAGGCACTGGCAGGATTCCTGTTTGATTCCGAGGAACATCTCATCCGGGTGGATATGTCT
GAATTCATGGAGAAGCATTCAGTCGCGCGCCTGATTGGCGCGCCGCCAGGCTATGTTGGCTATGAAGAAGGCGGTTATCT
GACTGAACAGATACGACGTAAGCCTTATTCCGTCATTCTGCTCGATGAAGTAGAAAAAGCACATCCGGACGTGTTTAATG
TGTTACTGCAGGTGCTGGACGACGGTCGGATGACGGATGGCCAGGGACGCACTGTAGACTTCAAAAACACGGTCATCGTC
ATGACTTCCAACTTAGGTTCACAAATGATCCAGCAGATGAGCGGCGATGATTACCAAGCCATCAAGCAAGCAGTAATGGG
AGAGGTTAAAACCTATTTCCGACCAGAGTTTATTAACCGTATTGATGAGGTGGTTGTTTTCCATTCGCTGGATGAAGCGC
ACATCAAGTCGATTGCCAAAATTCAGTTGAGTAATCTGGGAGCCCGATTGGCGCAAATGGAGATGAAACTGGTAGTTTCA
GAATCAGCTCTGGCAAAACTGGCTGAGGCTGGTTTTGATCCGATATTTGGTGCACGGCCTCTGAAACGTGCCATTCAGTC
TCAGATCGAAAATCCGCTGTCCAGAGAATTGCTGGAGGGTCATTTCTCTGCTGGAGATACTATCCTGGTTAAATATGGTG
ACGGTAGTATGCAGTTCTCGAAACAGCATTAA

Upstream 100 bases:

>100_bases
ATCTCCAGTTAAAAAATCAAATCGCGCCACCGGGTTGAAATTTCGCCACCACGCCATATAGTAAGGGCATTCCAATCTTT
ACTTCACTTCAGGAGATGAC

Downstream 100 bases:

>100_bases
GCCTGGCTTATGGCCAATCGCTGAAATTGAATGAAAGCAGATTGTTTCGGGTAGAATAATGATTATTATTATTTATATAG
ACAAAGTGCCATGTTCAGAG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MRFDKFTTKFQQALADAQSMALGQDHPYIESQHLLLALMQQEDGSIASLLQRAGVNVQPLRQALTQSLKLLPKVEGTGGE
INISRDLGNLLNLTDKEAQKRGDQYIASEMFLLAVLEDKGETGRLLKQYGVTRAALEQTIDSVRGGEQVTDAEAEGSREA
LKKYTLDLTERARSGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKNKRVLSL
DMAALLAGAKYRGEFEERLKTVLKELAQDEGRTIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYR
KYVEKDAALERRFQKVLVDEPGVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IRMEQDSKPEVMDKLDRRLIQLKIEREAVKKEKDEASKKRLALLEEEIEKLEREYADLDEILKAEKSRAKGSQEIKEELE
KLRREEEAARRKGDLQKASELLYGRIPQLETQLAEQLHHDESAEEATQPKLFRTQVGAEEIAEVVSRATGIPVSKMMQGE
REKLLFMEDKLHERVIGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALAGFLFDSEEHLIRVDMS
EFMEKHSVARLIGAPPGYVGYEEGGYLTEQIRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIV
MTSNLGSQMIQQMSGDDYQAIKQAVMGEVKTYFRPEFINRIDEVVVFHSLDEAHIKSIAKIQLSNLGARLAQMEMKLVVS
ESALAKLAEAGFDPIFGARPLKRAIQSQIENPLSRELLEGHFSAGDTILVKYGDGSMQFSKQH

Sequences:

>Translated_863_residues
MRFDKFTTKFQQALADAQSMALGQDHPYIESQHLLLALMQQEDGSIASLLQRAGVNVQPLRQALTQSLKLLPKVEGTGGE
INISRDLGNLLNLTDKEAQKRGDQYIASEMFLLAVLEDKGETGRLLKQYGVTRAALEQTIDSVRGGEQVTDAEAEGSREA
LKKYTLDLTERARSGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKNKRVLSL
DMAALLAGAKYRGEFEERLKTVLKELAQDEGRTIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYR
KYVEKDAALERRFQKVLVDEPGVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IRMEQDSKPEVMDKLDRRLIQLKIEREAVKKEKDEASKKRLALLEEEIEKLEREYADLDEILKAEKSRAKGSQEIKEELE
KLRREEEAARRKGDLQKASELLYGRIPQLETQLAEQLHHDESAEEATQPKLFRTQVGAEEIAEVVSRATGIPVSKMMQGE
REKLLFMEDKLHERVIGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALAGFLFDSEEHLIRVDMS
EFMEKHSVARLIGAPPGYVGYEEGGYLTEQIRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIV
MTSNLGSQMIQQMSGDDYQAIKQAVMGEVKTYFRPEFINRIDEVVVFHSLDEAHIKSIAKIQLSNLGARLAQMEMKLVVS
ESALAKLAEAGFDPIFGARPLKRAIQSQIENPLSRELLEGHFSAGDTILVKYGDGSMQFSKQH
>Mature_863_residues
MRFDKFTTKFQQALADAQSMALGQDHPYIESQHLLLALMQQEDGSIASLLQRAGVNVQPLRQALTQSLKLLPKVEGTGGE
INISRDLGNLLNLTDKEAQKRGDQYIASEMFLLAVLEDKGETGRLLKQYGVTRAALEQTIDSVRGGEQVTDAEAEGSREA
LKKYTLDLTERARSGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKNKRVLSL
DMAALLAGAKYRGEFEERLKTVLKELAQDEGRTIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYR
KYVEKDAALERRFQKVLVDEPGVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IRMEQDSKPEVMDKLDRRLIQLKIEREAVKKEKDEASKKRLALLEEEIEKLEREYADLDEILKAEKSRAKGSQEIKEELE
KLRREEEAARRKGDLQKASELLYGRIPQLETQLAEQLHHDESAEEATQPKLFRTQVGAEEIAEVVSRATGIPVSKMMQGE
REKLLFMEDKLHERVIGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALAGFLFDSEEHLIRVDMS
EFMEKHSVARLIGAPPGYVGYEEGGYLTEQIRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIV
MTSNLGSQMIQQMSGDDYQAIKQAVMGEVKTYFRPEFINRIDEVVVFHSLDEAHIKSIAKIQLSNLGARLAQMEMKLVVS
ESALAKLAEAGFDPIFGARPLKRAIQSQIENPLSRELLEGHFSAGDTILVKYGDGSMQFSKQH

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=325, Percent_Identity=36, Blast_Score=204, Evalue=3e-52,
Organism=Escherichia coli, GI1788943, Length=851, Percent_Identity=68.3901292596945, Blast_Score=1167, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=402, Percent_Identity=42.7860696517413, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6320464, Length=718, Percent_Identity=52.0891364902507, Blast_Score=746, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=860, Percent_Identity=43.8372093023256, Blast_Score=686, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96508; Mature: 96508

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDKFTTKFQQALADAQSMALGQDHPYIESQHLLLALMQQEDGSIASLLQRAGVNVQPL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
RQALTQSLKLLPKVEGTGGEINISRDLGNLLNLTDKEAQKRGDQYIASEMFLLAVLEDKG
HHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ETGRLLKQYGVTRAALEQTIDSVRGGEQVTDAEAEGSREALKKYTLDLTERARSGKLDPV
CHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHH
DMAALLAGAKYRGEFEERLKTVLKELAQDEGRTIVFIDELHTMVGAGKAEGAIDAGNMLK
HHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRKYVEKDAALERRFQKVLVDEPGVEATIAILRGLQEKYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIRMEQDSKPEVMDKLDRRLI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLKIEREAVKKEKDEASKKRLALLEEEIEKLEREYADLDEILKAEKSRAKGSQEIKEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KLRREEEAARRKGDLQKASELLYGRIPQLETQLAEQLHHDESAEEATQPKLFRTQVGAEE
HHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCHHH
IAEVVSRATGIPVSKMMQGEREKLLFMEDKLHERVIGQDEAVRLVSDAIRRSRSGLSDPN
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
RPYGSFLFLGPTGVGKTELCKALAGFLFDSEEHLIRVDMSEFMEKHSVARLIGAPPGYVG
CCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCC
YEEGGYLTEQIRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIV
CCCCCHHHHHHCCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEE
MTSNLGSQMIQQMSGDDYQAIKQAVMGEVKTYFRPEFINRIDEVVVFHSLDEAHIKSIAK
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHH
IQLSNLGARLAQMEMKLVVSESALAKLAEAGFDPIFGARPLKRAIQSQIENPLSRELLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
HFSAGDTILVKYGDGSMQFSKQH
HCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MRFDKFTTKFQQALADAQSMALGQDHPYIESQHLLLALMQQEDGSIASLLQRAGVNVQPL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
RQALTQSLKLLPKVEGTGGEINISRDLGNLLNLTDKEAQKRGDQYIASEMFLLAVLEDKG
HHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ETGRLLKQYGVTRAALEQTIDSVRGGEQVTDAEAEGSREALKKYTLDLTERARSGKLDPV
CHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCHHH
DMAALLAGAKYRGEFEERLKTVLKELAQDEGRTIVFIDELHTMVGAGKAEGAIDAGNMLK
HHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRKYVEKDAALERRFQKVLVDEPGVEATIAILRGLQEKYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVEITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIRMEQDSKPEVMDKLDRRLI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLKIEREAVKKEKDEASKKRLALLEEEIEKLEREYADLDEILKAEKSRAKGSQEIKEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KLRREEEAARRKGDLQKASELLYGRIPQLETQLAEQLHHDESAEEATQPKLFRTQVGAEE
HHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCHHH
IAEVVSRATGIPVSKMMQGEREKLLFMEDKLHERVIGQDEAVRLVSDAIRRSRSGLSDPN
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
RPYGSFLFLGPTGVGKTELCKALAGFLFDSEEHLIRVDMSEFMEKHSVARLIGAPPGYVG
CCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCC
YEEGGYLTEQIRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIV
CCCCCHHHHHHCCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEE
MTSNLGSQMIQQMSGDDYQAIKQAVMGEVKTYFRPEFINRIDEVVVFHSLDEAHIKSIAK
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHH
IQLSNLGARLAQMEMKLVVSESALAKLAEAGFDPIFGARPLKRAIQSQIENPLSRELLEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
HFSAGDTILVKYGDGSMQFSKQH
HCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]