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The map label for this gene is yerD [H]

Identifier: 114330750

GI number: 114330750

Start: 752958

End: 754451

Strand: Reverse

Name: yerD [H]

Synonym: Neut_0735

Alternate gene names: 114330750

Gene position: 754451-752958 (Counterclockwise)

Preceding gene: 114330751

Following gene: 114330749

Centisome position: 28.35

GC content: 51.74

Gene sequence:

>1494_bases
ATGTCAGACAGCCTGATCAATCTAATTATTTTCAGTACTGCCACTATTTTAGGGCTAATTATCGTGGGTGCCATAGTCAT
CTTTTTCATCCGCGATGCTACCCAGAAAAAGCACACCATATTGCGCAATTATCCCATCATCGGGCGATTGCGCTATTTTT
TTGAAACTCAGGGAAAGTATTTCCGCCAATATTTCTTTACCGGAGATCGTGATGAAATGCCATTCAATCGCGCTACTCGG
AGCTGGATCTATAAAAATGCCAAAAATATGGAAAGTACTGTTGGCTTTGGCTCCTCGTACGATCTGTGCGAACCCGGGGC
GCTCATTTTTGTCAATGCCGCTTTTCCCATACTGGAAGAAGATCAGCTGCCCATCCCCGCCCTGTCCATTGGTGAAGGTT
ACTGCGACCAGCCCTTTCTGGCGCAATCCATCATCAACATCAGCGGTATGAGCTATGGTGCCATTTCCGCACCAGCTGTG
CGTGCGCTTTCGCTTGGTGCGGCTAAGGCCGGATGCTGGCTAAGTACAGGCGAAGGGGGATTAGCTCCTTTTCACCTGGA
AGGGAAATGCGACATCATCATGCAAATCGGCACAGCCAAATACGGTATCCGGGATGAGCAGGGAAAGTTTTCTGCCACCC
GTGCACGGGAAATTGCCCAATCCGTCAAGGCATTTGAAATCAAACTTTCGCAAGGGGCAAAACCAGGTAAAGGCGGCATG
CTGCCGGCATGCAAAGTCAGCCCGGAAATTGCCGCTATCCGGGGCATTCCCCCCTGGCAGGATTCTATCAGTCCCAACCG
GCATCACGATGTTGGTAATATTGAAGAACTGCTGGATCAAATTGCCTTTATCCGGGAACTGACCGGACGACCAGTTGGCA
TCAAAACTGCTATTGGTGGCTGGCATTTCATCAATGAGTTGTGCGATGTCATCCTGCGTAGAGGGCTGGAATATGCCCCC
GATTTCCTGACAATTGATGGCGGTGAAGGTGGCAGCGGCGCAGCTCCACAGGCGCTGATCGATTATGCCGGGCTGCCGAT
TACCGAGGCACTGCCGCGGGTGGTCGATGCTCTGATCGAATCCGGCCTGAGACAGCGTATCCGCGTCGTTGCAGCAGGGA
AACTGGTTACTTCTGCCAAATGTGCATGGGCATTATGTGCGGGTGCAGATTTCGTCAATACCGCTCGGGGATTCATGTTT
TCCCTGGGCTGCATTCAGGCCATGCGCTGTCACCTGAACACCTGCCCGACCGGTATCACTACCAATAACCCGCGCCTGCA
ACGTGGCCTGGTCGTGGAAGAAAAATATCTGCGAGTGGCCAATTACGCCACCAACGTTAATCACGAAATCAGCATGATTG
CCCATTCTTGCGGATTGCGGCATGCCCGTGAACTACGTCGGGAACATATCCGCATCGTGGAAACGGCCGGAAAGAGCATT
GCGCTGAACATGCTATATCCTTACCCGGAGATAAGAAATCAACGCTCTCATTAG

Upstream 100 bases:

>100_bases
TGCCAATATCTTCATCCAGCGCTTGTCGAACCTGCTTGCTGATTGCTTCACTTTGCAACATGCTTCACACTCATCTATTT
AGAACATCATTCCAGACACC

Downstream 100 bases:

>100_bases
CACCTTGACAGACAATGTGAAACGATCCACCAATAAGGCAATTAACTGCGCCAAAGAAGCAGCATGCACGGACAGCTGAA
ACAAACAGCCAAGAATCAAG

Product: ferredoxin-dependent glutamate synthase

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSDSLINLIIFSTATILGLIIVGAIVIFFIRDATQKKHTILRNYPIIGRLRYFFETQGKYFRQYFFTGDRDEMPFNRATR
SWIYKNAKNMESTVGFGSSYDLCEPGALIFVNAAFPILEEDQLPIPALSIGEGYCDQPFLAQSIINISGMSYGAISAPAV
RALSLGAAKAGCWLSTGEGGLAPFHLEGKCDIIMQIGTAKYGIRDEQGKFSATRAREIAQSVKAFEIKLSQGAKPGKGGM
LPACKVSPEIAAIRGIPPWQDSISPNRHHDVGNIEELLDQIAFIRELTGRPVGIKTAIGGWHFINELCDVILRRGLEYAP
DFLTIDGGEGGSGAAPQALIDYAGLPITEALPRVVDALIESGLRQRIRVVAAGKLVTSAKCAWALCAGADFVNTARGFMF
SLGCIQAMRCHLNTCPTGITTNNPRLQRGLVVEEKYLRVANYATNVNHEISMIAHSCGLRHARELRREHIRIVETAGKSI
ALNMLYPYPEIRNQRSH

Sequences:

>Translated_497_residues
MSDSLINLIIFSTATILGLIIVGAIVIFFIRDATQKKHTILRNYPIIGRLRYFFETQGKYFRQYFFTGDRDEMPFNRATR
SWIYKNAKNMESTVGFGSSYDLCEPGALIFVNAAFPILEEDQLPIPALSIGEGYCDQPFLAQSIINISGMSYGAISAPAV
RALSLGAAKAGCWLSTGEGGLAPFHLEGKCDIIMQIGTAKYGIRDEQGKFSATRAREIAQSVKAFEIKLSQGAKPGKGGM
LPACKVSPEIAAIRGIPPWQDSISPNRHHDVGNIEELLDQIAFIRELTGRPVGIKTAIGGWHFINELCDVILRRGLEYAP
DFLTIDGGEGGSGAAPQALIDYAGLPITEALPRVVDALIESGLRQRIRVVAAGKLVTSAKCAWALCAGADFVNTARGFMF
SLGCIQAMRCHLNTCPTGITTNNPRLQRGLVVEEKYLRVANYATNVNHEISMIAHSCGLRHARELRREHIRIVETAGKSI
ALNMLYPYPEIRNQRSH
>Mature_496_residues
SDSLINLIIFSTATILGLIIVGAIVIFFIRDATQKKHTILRNYPIIGRLRYFFETQGKYFRQYFFTGDRDEMPFNRATRS
WIYKNAKNMESTVGFGSSYDLCEPGALIFVNAAFPILEEDQLPIPALSIGEGYCDQPFLAQSIINISGMSYGAISAPAVR
ALSLGAAKAGCWLSTGEGGLAPFHLEGKCDIIMQIGTAKYGIRDEQGKFSATRAREIAQSVKAFEIKLSQGAKPGKGGML
PACKVSPEIAAIRGIPPWQDSISPNRHHDVGNIEELLDQIAFIRELTGRPVGIKTAIGGWHFINELCDVILRRGLEYAPD
FLTIDGGEGGSGAAPQALIDYAGLPITEALPRVVDALIESGLRQRIRVVAAGKLVTSAKCAWALCAGADFVNTARGFMFS
LGCIQAMRCHLNTCPTGITTNNPRLQRGLVVEEKYLRVANYATNVNHEISMIAHSCGLRHARELRREHIRIVETAGKSIA
LNMLYPYPEIRNQRSH

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=222, Percent_Identity=35.5855855855856, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=319, Percent_Identity=30.4075235109718, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320030, Length=323, Percent_Identity=31.2693498452012, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI28574881, Length=356, Percent_Identity=29.2134831460674, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24665539, Length=356, Percent_Identity=29.2134831460674, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 54479; Mature: 54347

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLINLIIFSTATILGLIIVGAIVIFFIRDATQKKHTILRNYPIIGRLRYFFETQGKY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHH
FRQYFFTGDRDEMPFNRATRSWIYKNAKNMESTVGFGSSYDLCEPGALIFVNAAFPILEE
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
DQLPIPALSIGEGYCDQPFLAQSIINISGMSYGAISAPAVRALSLGAAKAGCWLSTGEGG
CCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCC
LAPFHLEGKCDIIMQIGTAKYGIRDEQGKFSATRAREIAQSVKAFEIKLSQGAKPGKGGM
CCEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
LPACKVSPEIAAIRGIPPWQDSISPNRHHDVGNIEELLDQIAFIRELTGRPVGIKTAIGG
CCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHH
WHFINELCDVILRRGLEYAPDFLTIDGGEGGSGAAPQALIDYAGLPITEALPRVVDALIE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
SGLRQRIRVVAAGKLVTSAKCAWALCAGADFVNTARGFMFSLGCIQAMRCHLNTCPTGIT
HHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TNNPRLQRGLVVEEKYLRVANYATNVNHEISMIAHSCGLRHARELRREHIRIVETAGKSI
CCCCCHHHCEEHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEE
ALNMLYPYPEIRNQRSH
EEEEECCCHHHCCCCCC
>Mature Secondary Structure 
SDSLINLIIFSTATILGLIIVGAIVIFFIRDATQKKHTILRNYPIIGRLRYFFETQGKY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHH
FRQYFFTGDRDEMPFNRATRSWIYKNAKNMESTVGFGSSYDLCEPGALIFVNAAFPILEE
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
DQLPIPALSIGEGYCDQPFLAQSIINISGMSYGAISAPAVRALSLGAAKAGCWLSTGEGG
CCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCC
LAPFHLEGKCDIIMQIGTAKYGIRDEQGKFSATRAREIAQSVKAFEIKLSQGAKPGKGGM
CCEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
LPACKVSPEIAAIRGIPPWQDSISPNRHHDVGNIEELLDQIAFIRELTGRPVGIKTAIGG
CCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHH
WHFINELCDVILRRGLEYAPDFLTIDGGEGGSGAAPQALIDYAGLPITEALPRVVDALIE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
SGLRQRIRVVAAGKLVTSAKCAWALCAGADFVNTARGFMFSLGCIQAMRCHLNTCPTGIT
HHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TNNPRLQRGLVVEEKYLRVANYATNVNHEISMIAHSCGLRHARELRREHIRIVETAGKSI
CCCCCHHHCEEHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEE
ALNMLYPYPEIRNQRSH
EEEEECCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]