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The map label for this gene is murE [H]

Identifier: 114330278

GI number: 114330278

Start: 265262

End: 266824

Strand: Reverse

Name: murE [H]

Synonym: Neut_0251

Alternate gene names: 114330278

Gene position: 266824-265262 (Counterclockwise)

Preceding gene: 114330279

Following gene: 114330277

Centisome position: 10.03

GC content: 50.03

Gene sequence:

>1563_bases
ATGAGCACTTCGTCCCGCTTTGGATCAGATGAAACTTATCTTGCTTGCTTGCTGGATCGGCTGAGCGTCAAGATACAACG
TCTGGCGGCAGACAGCCGCACCCTGAAACCGGGCGATACCTTCCTGGCTTGTGCTGGCGAACAGCATGATGCACGCAACG
ATATTCCACAAGCAATTGCATTGGGCGTCAATGCAGTTATCTGGGAAAAACAAGGATTTTCCTGGAAACCGGAGTGGAAG
ATACCCAATCTGGGTATCTCTGGATTACGCCATGAAGCCGGGAAAATTGCCAGCCAGGTGTACGACCACCCATCCAGACA
TTTACGGCTAGTTGGAATCACAGGCACCAACGGAAAAACTACGTGTAGTCACTGGTACGCCCAGACCATGATGACACTGG
GGGAAAAGACAGCCATCATTGGCACGCTGGGACATGGCTTCCCGGGAGCGTTGAATCATGCCAACCATACAACGCCGGAT
GCAGTATATCTGCAGCAACTGATGGCTGACTATTTACAGCACGGCGCTCGCTCCCTGGTTATGGAAGCTTCTTCACATGG
TCTTTCTCAAGACAGGCTGGCTGGTTCGGAATTTTCGATTGCCGTACTGACCAATCTGACGCGTGACCACCTGGATTATC
ATGGCAGTATGGATGCCTATGCTGCTGCTAAGGCAAAACTCTTTTTCTGGGAGGGGCTGCAACACGCTGTCCTTAATCTG
GACGAGGTACTGGGCGTGGAACTATCACAACAGCTGGCCTGGAAAGACGTGTCGATCATTGGTTACGGTTTCAGGCAACC
TGATCCATCAGCCCGTACAACCGGTAACCAAAAAATACTCTACGGAAGTAATCTGCGATTTACCACACAGCATATTAGCT
TCGATGTTGAATTTTGTAATCGGCGCGCCAGTCTGCAATGTAATGTAACCGGTCGTTATAACGCATATAACCTATTGGCT
GTACTGGCCACTTTACTTGCCAGTAATGTTGATCTTGATGATGCAATAACTGCCTTGCAACAAGTACAACCAATTCCAGG
GCGAATGGAAAAACTGGGAGGAGGTGATCAGCCCATCGTAATCGTTGATTATGCGCATACACCCGATGCATTGAACGAAG
TATTAACCGGCTTGCGCGAAACACTGGCCGGTACGCGCATGAAAAAACGGATACGAAAAAACCAGGCAAAACTGGTTTGC
GTGATCGGTTGCGGAGGAGATAGAGATCGCGGCAAGCGCCCGTTGATTGGAGAGATTGCTTCACACCTGGCTGATGAAGT
CATTATTACCAGCGATAATCCGCGCAATGAAAATCCGGCAGATATTATCAATGAAATCATGCTTGGGGCCAGTGGCAAGC
ACTGCACGGCTGAAGCGGATCGCACTGCAGCCATTTACCGGGCTATTCATGGTGCGCGCAAGGGCGATATTGTATTGATC
GCCGGCAAGGGGGCAGAAACTCATCAGGAAATACAAGGAAAAAAATATCCATTCGATGATCGCGAGGTAGTGCGGCAGGT
ATTGCATGACCTGGCTGGTCCAGAATTGCAGGTGCAGGGATGA

Upstream 100 bases:

>100_bases
GGGTAATGGAAGGTACGTTGCGCATATTGAACGTGCCGTTTGATGAGCCACTGGGCAATCTGGTTATATCACCTGTTCCT
GCAGAGCTGGAAGATAAGGG

Downstream 100 bases:

>100_bases
TGAGCGTACAGGAAGCTGCACTAGCCCTGCATGCAAATTCAAATTGGTCTGGTGGCAACCCGATATTCACTGGGGTCAGC
ACAGACAGTCGCACCCTGAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSTSSRFGSDETYLACLLDRLSVKIQRLAADSRTLKPGDTFLACAGEQHDARNDIPQAIALGVNAVIWEKQGFSWKPEWK
IPNLGISGLRHEAGKIASQVYDHPSRHLRLVGITGTNGKTTCSHWYAQTMMTLGEKTAIIGTLGHGFPGALNHANHTTPD
AVYLQQLMADYLQHGARSLVMEASSHGLSQDRLAGSEFSIAVLTNLTRDHLDYHGSMDAYAAAKAKLFFWEGLQHAVLNL
DEVLGVELSQQLAWKDVSIIGYGFRQPDPSARTTGNQKILYGSNLRFTTQHISFDVEFCNRRASLQCNVTGRYNAYNLLA
VLATLLASNVDLDDAITALQQVQPIPGRMEKLGGGDQPIVIVDYAHTPDALNEVLTGLRETLAGTRMKKRIRKNQAKLVC
VIGCGGDRDRGKRPLIGEIASHLADEVIITSDNPRNENPADIINEIMLGASGKHCTAEADRTAAIYRAIHGARKGDIVLI
AGKGAETHQEIQGKKYPFDDREVVRQVLHDLAGPELQVQG

Sequences:

>Translated_520_residues
MSTSSRFGSDETYLACLLDRLSVKIQRLAADSRTLKPGDTFLACAGEQHDARNDIPQAIALGVNAVIWEKQGFSWKPEWK
IPNLGISGLRHEAGKIASQVYDHPSRHLRLVGITGTNGKTTCSHWYAQTMMTLGEKTAIIGTLGHGFPGALNHANHTTPD
AVYLQQLMADYLQHGARSLVMEASSHGLSQDRLAGSEFSIAVLTNLTRDHLDYHGSMDAYAAAKAKLFFWEGLQHAVLNL
DEVLGVELSQQLAWKDVSIIGYGFRQPDPSARTTGNQKILYGSNLRFTTQHISFDVEFCNRRASLQCNVTGRYNAYNLLA
VLATLLASNVDLDDAITALQQVQPIPGRMEKLGGGDQPIVIVDYAHTPDALNEVLTGLRETLAGTRMKKRIRKNQAKLVC
VIGCGGDRDRGKRPLIGEIASHLADEVIITSDNPRNENPADIINEIMLGASGKHCTAEADRTAAIYRAIHGARKGDIVLI
AGKGAETHQEIQGKKYPFDDREVVRQVLHDLAGPELQVQG
>Mature_519_residues
STSSRFGSDETYLACLLDRLSVKIQRLAADSRTLKPGDTFLACAGEQHDARNDIPQAIALGVNAVIWEKQGFSWKPEWKI
PNLGISGLRHEAGKIASQVYDHPSRHLRLVGITGTNGKTTCSHWYAQTMMTLGEKTAIIGTLGHGFPGALNHANHTTPDA
VYLQQLMADYLQHGARSLVMEASSHGLSQDRLAGSEFSIAVLTNLTRDHLDYHGSMDAYAAAKAKLFFWEGLQHAVLNLD
EVLGVELSQQLAWKDVSIIGYGFRQPDPSARTTGNQKILYGSNLRFTTQHISFDVEFCNRRASLQCNVTGRYNAYNLLAV
LATLLASNVDLDDAITALQQVQPIPGRMEKLGGGDQPIVIVDYAHTPDALNEVLTGLRETLAGTRMKKRIRKNQAKLVCV
IGCGGDRDRGKRPLIGEIASHLADEVIITSDNPRNENPADIINEIMLGASGKHCTAEADRTAAIYRAIHGARKGDIVLIA
GKGAETHQEIQGKKYPFDDREVVRQVLHDLAGPELQVQG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=498, Percent_Identity=38.5542168674699, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 56867; Mature: 56736

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSRFGSDETYLACLLDRLSVKIQRLAADSRTLKPGDTFLACAGEQHDARNDIPQAIA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
LGVNAVIWEKQGFSWKPEWKIPNLGISGLRHEAGKIASQVYDHPSRHLRLVGITGTNGKT
HCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
TCSHWYAQTMMTLGEKTAIIGTLGHGFPGALNHANHTTPDAVYLQQLMADYLQHGARSLV
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MEASSHGLSQDRLAGSEFSIAVLTNLTRDHLDYHGSMDAYAAAKAKLFFWEGLQHAVLNL
HHHHHCCCCHHHCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHEEHHHHHHHHHHCH
DEVLGVELSQQLAWKDVSIIGYGFRQPDPSARTTGNQKILYGSNLRFTTQHISFDVEFCN
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEHHHHC
RRASLQCNVTGRYNAYNLLAVLATLLASNVDLDDAITALQQVQPIPGRMEKLGGGDQPIV
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEE
IVDYAHTPDALNEVLTGLRETLAGTRMKKRIRKNQAKLVCVIGCGGDRDRGKRPLIGEIA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHH
SHLADEVIITSDNPRNENPADIINEIMLGASGKHCTAEADRTAAIYRAIHGARKGDIVLI
HHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEE
AGKGAETHQEIQGKKYPFDDREVVRQVLHDLAGPELQVQG
ECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
STSSRFGSDETYLACLLDRLSVKIQRLAADSRTLKPGDTFLACAGEQHDARNDIPQAIA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHH
LGVNAVIWEKQGFSWKPEWKIPNLGISGLRHEAGKIASQVYDHPSRHLRLVGITGTNGKT
HCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
TCSHWYAQTMMTLGEKTAIIGTLGHGFPGALNHANHTTPDAVYLQQLMADYLQHGARSLV
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MEASSHGLSQDRLAGSEFSIAVLTNLTRDHLDYHGSMDAYAAAKAKLFFWEGLQHAVLNL
HHHHHCCCCHHHCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHEEHHHHHHHHHHCH
DEVLGVELSQQLAWKDVSIIGYGFRQPDPSARTTGNQKILYGSNLRFTTQHISFDVEFCN
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEHHHHC
RRASLQCNVTGRYNAYNLLAVLATLLASNVDLDDAITALQQVQPIPGRMEKLGGGDQPIV
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEE
IVDYAHTPDALNEVLTGLRETLAGTRMKKRIRKNQAKLVCVIGCGGDRDRGKRPLIGEIA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHH
SHLADEVIITSDNPRNENPADIINEIMLGASGKHCTAEADRTAAIYRAIHGARKGDIVLI
HHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEE
AGKGAETHQEIQGKKYPFDDREVVRQVLHDLAGPELQVQG
ECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]