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The map label for this gene is penA [H]

Identifier: 114330279

GI number: 114330279

Start: 266821

End: 268560

Strand: Reverse

Name: penA [H]

Synonym: Neut_0252

Alternate gene names: 114330279

Gene position: 268560-266821 (Counterclockwise)

Preceding gene: 114330280

Following gene: 114330278

Centisome position: 10.09

GC content: 49.89

Gene sequence:

>1740_bases
ATGAAAGTTTTGTTCAATCCTTCCCGTAAAACAATCTCTCCTGTTCCAGAATGGCGTTCACGCTTGGTGCAGGGACTTCT
ACTGACGTGCCTGATCATACTAATCTCCCGGTCAATTTACTTACAGGCACTGAACAAGGATTTTCTTCAGCAACAAGGGC
AGTCACGTCACGAGCGGGTTATCGAACAAAATATGCAGCGCGGCAATATCAAGGATCGCAATGGTGAAATCCTTGCTGTT
AGCGCACCTGTCAGATCTGTATGGATCGAACCTCAAAGAGTAAATGCAACACCGGAACAAATTAGACAATTAGCCAGTTT
GACGGGCGTAGAAGAAGCAGTCATCCAGCAGCGCATTCGCAGCGGGAAAAAATTTGTATACCTGAGACGGCAGCTCCCTC
CAGAATTAGCGGAAAAGGTAGCTGAACTCAATATAAAAGGAGTAAATCTCAAGCACGAGTTCTATCGTTATTATCCAGCA
CGCGAGCTGGCCGCGCATATTCTGGGCTTCACTGATATAGATGGACGGGGGCAGGAAGGAATGGAGCTGGCGTGGCAGGA
CATGCTGACCGGCGAGGATGGTAAACGGCGCGTGATTAAGGACCGGATCGGACGTATTGTGGAAGATGTCGAGCAGATTC
AATCTCCGAAACCGGGCCAGGATGTCGTTCTGTCAATTGACAGTAAAATACAATACCTCGCTTACCGGGAATTGGCACGC
GCAGTAAAAGAACAGCACGCTAAAGCAGGTAGCATCGTAGCGTTGGATGTACAAACTGGTGAAGTACTGGCAATGGCGAA
CTACCCGGCTTTCAATCCCAATCAGCGCGCCAGCATGAATAATGAAGTAATTCGTAATCGCGTGCTGGTCGATACATTCG
AACCTGGTTCAACATTAAAGCCATTCGTCGTGGCTGTGGCAATGGAAACCGGCCGGATCAAGCCGGATACGCTGATGGAG
ACTGCTGGAGGCAAACTGAAAATAGGCAGAGCTGTCATACATGACGTGCGTGACAAGGGCAACCTGACCGTTTCCCAGGT
TATTCAGGCTTCCAGTAATGTTGGCGCTGCAAAAATTGCTTTGTTGCTCCCGCCAAAAACTTTCTGGGAAATGCTCAACC
GCTCAGGTTTTGGGACTGAAACCGGCATTGGATTTCCCGGCGAGGCAAGCGGACGGTTACGTGCATATAATACCTGGCGA
CCGATCGAGCAGGCCACCATGTCCTATGGCCATGGCATTTCAGCCAGTTTAATGCAGCTGGCGCGTGCTTATACGCTGTT
CGCCACTGATGGAGAATTAAAGCCTGTCACACTGCTAAAGCGTGATATGCCTGCCGTTGGCCAGAAAGTCATTTCGCACG
AAACTGCACAATCAGTCCGAAAAATGCTGGAACTTGCCGTTCAACCTGAAGGCACAGGCAATAGTGCACGCATCAGCGGC
TACCGTGTTGCAGGAAAAACCGGTACCGCACACAAACGCCTGAAAGGTCAGAAAGGTTATGCCAAAGATCGCTATATCTC
TTCATTTGTCGGCTTCGCTCCGGCGTCTGATCCGCGCGTAATCATCGCCATCATGATTGATGAACCCTCAAGCGGAAATT
ATTATGGCGGTACAGTAGCCGCCCCTGTCTTCAGTCGGGTAATGGAAGGTACGTTGCGCATATTGAACGTGCCGTTTGAT
GAGCCACTGGGCAATCTGGTTATATCACCTGTTCCTGCAGAGCTGGAAGATAAGGGATGA

Upstream 100 bases:

>100_bases
GTGTAGAAAAAATCGCCCGGAAAGAACTTGGCATGATTACGCCACCTACGACCGGAAATATTCTGGCTATTCATCCTGAT
TACACTCCGGGCACAACCGA

Downstream 100 bases:

>100_bases
GCACTTCGTCCCGCTTTGGATCAGATGAAACTTATCTTGCTTGCTTGCTGGATCGGCTGAGCGTCAAGATACAACGTCTG
GCGGCAGACAGCCGCACCCT

Product: peptidoglycan glycosyltransferase

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MKVLFNPSRKTISPVPEWRSRLVQGLLLTCLIILISRSIYLQALNKDFLQQQGQSRHERVIEQNMQRGNIKDRNGEILAV
SAPVRSVWIEPQRVNATPEQIRQLASLTGVEEAVIQQRIRSGKKFVYLRRQLPPELAEKVAELNIKGVNLKHEFYRYYPA
RELAAHILGFTDIDGRGQEGMELAWQDMLTGEDGKRRVIKDRIGRIVEDVEQIQSPKPGQDVVLSIDSKIQYLAYRELAR
AVKEQHAKAGSIVALDVQTGEVLAMANYPAFNPNQRASMNNEVIRNRVLVDTFEPGSTLKPFVVAVAMETGRIKPDTLME
TAGGKLKIGRAVIHDVRDKGNLTVSQVIQASSNVGAAKIALLLPPKTFWEMLNRSGFGTETGIGFPGEASGRLRAYNTWR
PIEQATMSYGHGISASLMQLARAYTLFATDGELKPVTLLKRDMPAVGQKVISHETAQSVRKMLELAVQPEGTGNSARISG
YRVAGKTGTAHKRLKGQKGYAKDRYISSFVGFAPASDPRVIIAIMIDEPSSGNYYGGTVAAPVFSRVMEGTLRILNVPFD
EPLGNLVISPVPAELEDKG

Sequences:

>Translated_579_residues
MKVLFNPSRKTISPVPEWRSRLVQGLLLTCLIILISRSIYLQALNKDFLQQQGQSRHERVIEQNMQRGNIKDRNGEILAV
SAPVRSVWIEPQRVNATPEQIRQLASLTGVEEAVIQQRIRSGKKFVYLRRQLPPELAEKVAELNIKGVNLKHEFYRYYPA
RELAAHILGFTDIDGRGQEGMELAWQDMLTGEDGKRRVIKDRIGRIVEDVEQIQSPKPGQDVVLSIDSKIQYLAYRELAR
AVKEQHAKAGSIVALDVQTGEVLAMANYPAFNPNQRASMNNEVIRNRVLVDTFEPGSTLKPFVVAVAMETGRIKPDTLME
TAGGKLKIGRAVIHDVRDKGNLTVSQVIQASSNVGAAKIALLLPPKTFWEMLNRSGFGTETGIGFPGEASGRLRAYNTWR
PIEQATMSYGHGISASLMQLARAYTLFATDGELKPVTLLKRDMPAVGQKVISHETAQSVRKMLELAVQPEGTGNSARISG
YRVAGKTGTAHKRLKGQKGYAKDRYISSFVGFAPASDPRVIIAIMIDEPSSGNYYGGTVAAPVFSRVMEGTLRILNVPFD
EPLGNLVISPVPAELEDKG
>Mature_579_residues
MKVLFNPSRKTISPVPEWRSRLVQGLLLTCLIILISRSIYLQALNKDFLQQQGQSRHERVIEQNMQRGNIKDRNGEILAV
SAPVRSVWIEPQRVNATPEQIRQLASLTGVEEAVIQQRIRSGKKFVYLRRQLPPELAEKVAELNIKGVNLKHEFYRYYPA
RELAAHILGFTDIDGRGQEGMELAWQDMLTGEDGKRRVIKDRIGRIVEDVEQIQSPKPGQDVVLSIDSKIQYLAYRELAR
AVKEQHAKAGSIVALDVQTGEVLAMANYPAFNPNQRASMNNEVIRNRVLVDTFEPGSTLKPFVVAVAMETGRIKPDTLME
TAGGKLKIGRAVIHDVRDKGNLTVSQVIQASSNVGAAKIALLLPPKTFWEMLNRSGFGTETGIGFPGEASGRLRAYNTWR
PIEQATMSYGHGISASLMQLARAYTLFATDGELKPVTLLKRDMPAVGQKVISHETAQSVRKMLELAVQPEGTGNSARISG
YRVAGKTGTAHKRLKGQKGYAKDRYISSFVGFAPASDPRVIIAIMIDEPSSGNYYGGTVAAPVFSRVMEGTLRILNVPFD
EPLGNLVISPVPAELEDKG

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=551, Percent_Identity=39.5644283121597, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786854, Length=600, Percent_Identity=23.8333333333333, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 64016; Mature: 64016

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00850 GLY_RADICAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLFNPSRKTISPVPEWRSRLVQGLLLTCLIILISRSIYLQALNKDFLQQQGQSRHERV
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IEQNMQRGNIKDRNGEILAVSAPVRSVWIEPQRVNATPEQIRQLASLTGVEEAVIQQRIR
HHHHHHCCCCCCCCCCEEEEECCHHHHCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
SGKKFVYLRRQLPPELAEKVAELNIKGVNLKHEFYRYYPARELAAHILGFTDIDGRGQEG
CCCEEEEEHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
MELAWQDMLTGEDGKRRVIKDRIGRIVEDVEQIQSPKPGQDVVLSIDSKIQYLAYRELAR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHH
AVKEQHAKAGSIVALDVQTGEVLAMANYPAFNPNQRASMNNEVIRNRVLVDTFEPGSTLK
HHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCEEEEECCCCCCCC
PFVVAVAMETGRIKPDTLMETAGGKLKIGRAVIHDVRDKGNLTVSQVIQASSNVGAAKIA
CEEEEEEHHCCCCCCHHHHHCCCCCEEECHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEE
LLLPPKTFWEMLNRSGFGTETGIGFPGEASGRLRAYNTWRPIEQATMSYGHGISASLMQL
EEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHH
ARAYTLFATDGELKPVTLLKRDMPAVGQKVISHETAQSVRKMLELAVQPEGTGNSARISG
HHHHEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
YRVAGKTGTAHKRLKGQKGYAKDRYISSFVGFAPASDPRVIIAIMIDEPSSGNYYGGTVA
EEEECCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHH
APVFSRVMEGTLRILNVPFDEPLGNLVISPVPAELEDKG
HHHHHHHHHCCCEEEECCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MKVLFNPSRKTISPVPEWRSRLVQGLLLTCLIILISRSIYLQALNKDFLQQQGQSRHERV
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IEQNMQRGNIKDRNGEILAVSAPVRSVWIEPQRVNATPEQIRQLASLTGVEEAVIQQRIR
HHHHHHCCCCCCCCCCEEEEECCHHHHCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
SGKKFVYLRRQLPPELAEKVAELNIKGVNLKHEFYRYYPARELAAHILGFTDIDGRGQEG
CCCEEEEEHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
MELAWQDMLTGEDGKRRVIKDRIGRIVEDVEQIQSPKPGQDVVLSIDSKIQYLAYRELAR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHH
AVKEQHAKAGSIVALDVQTGEVLAMANYPAFNPNQRASMNNEVIRNRVLVDTFEPGSTLK
HHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCEEEEECCCCCCCC
PFVVAVAMETGRIKPDTLMETAGGKLKIGRAVIHDVRDKGNLTVSQVIQASSNVGAAKIA
CEEEEEEHHCCCCCCHHHHHCCCCCEEECHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEE
LLLPPKTFWEMLNRSGFGTETGIGFPGEASGRLRAYNTWRPIEQATMSYGHGISASLMQL
EEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCHHHHHHH
ARAYTLFATDGELKPVTLLKRDMPAVGQKVISHETAQSVRKMLELAVQPEGTGNSARISG
HHHHEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
YRVAGKTGTAHKRLKGQKGYAKDRYISSFVGFAPASDPRVIIAIMIDEPSSGNYYGGTVA
EEEECCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHH
APVFSRVMEGTLRILNVPFDEPLGNLVISPVPAELEDKG
HHHHHHHHHCCCEEEECCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]