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The map label for this gene is murF [H]

Identifier: 114330277

GI number: 114330277

Start: 263892

End: 265265

Strand: Reverse

Name: murF [H]

Synonym: Neut_0250

Alternate gene names: 114330277

Gene position: 265265-263892 (Counterclockwise)

Preceding gene: 114330278

Following gene: 114330276

Centisome position: 9.97

GC content: 51.67

Gene sequence:

>1374_bases
ATGATGAGCGTACAGGAAGCTGCACTAGCCCTGCATGCAAATTCAAATTGGTCTGGTGGCAACCCGATATTCACTGGGGT
CAGCACAGACAGTCGCACCCTGAGACCGGGCGATCTTTTCATCGCGCTATCCGGCGAGCAGTTTGACGGTCAGCACTTCA
TTTCTACTGCCATCGAAAAAGGTGCCGTTGCCGCAATGGTTTCGACCGATACGGTCATCGTTCCGACAAAATCTGATTTC
GGCTGGATCAAAGTAAACGACACTCGGCTTGGCTTCGGCCAATTGGCCGCAAACTGGCGTCAGCACTTTACACTCCCGCT
GGTTGCCGTGACAGGTAGTAATGGCAAAACCACCGTCAAGGAAATGATTGCAGCTATTTTCAGACATGAATTCGGATCAG
GAAACGTTCTTGCAACGACCGGCAACCTGAATAACGACATTGGTGTTCCACAAATGCTGTTGCAGCTCAATACCGGGCAT
ACTTGCGCGGTTATTGAAATGGGCATGAACCACGCTGGCGAAATTGCCTATCTCAGTCAACTGGCTGCACCCACGATTGC
CGTCATCACCAATGCCGGCACCGCACATATTGAACACCTGGGTACAACCGAAGCGATTGCCCGTGCCAAGGGGGAAATCT
TTACAGGGCTGGGAGCACAGGGCATTGCAATCATCAACGCGGATGATGCTTATGCCCAGCTGTGGCGACAACTGGCAGGA
AGCAGACAGATTCTGGATTTCGGCATAACGAATCCTGCTGCAATCCGTGCGCAACAAATGTCCAGCTCATCAGAAGCTGC
ATGGTTGTTGCAACTGCCAGACGACAAAGTTGAAATAACGCTGCAGGTACCAGGCCGTCACAATATTTACAACGCACTGG
CTGCAGCGGCAGCTGCTACGGCTGCCGGCATCAGCACAACCTCCATTGCCGAGGGTCTGCATAACTTCATGGGCACTCCT
GGGCGTTTGCAGAAAAAAGTGGGGCTATATCAATCTATTCTGATTGATGACACCTACAATGCCAACCCGGATTCCATGCA
GGCAGCGCTGAATGTTCTGGCAGAGATGCCCGGCAAAAAAATTCTGATTATGGGGGACATGGGGGAGCTCGGTACGGATG
CTGCAGGATTCCACTACACCATCGGCCAACAGGCTGCAAAAGCTGGCATAGATGCGTTACTGGCACTGGGAGAATTAAGC
CAGCAAACCGTTGCAGGATTTGGCAGCGGGGCACAATATTTTGCCGATCTGGATACATTGCTGGAAAAAGCAAAAGACTG
CCTGGGAAAGAATGTTTCTGTTCTGGTCAAAGGCTCGCGATTCATGCGCATGGAGCGTGTGATCGAGCAACTGCAGGATA
GATATGTTGAGTAG

Upstream 100 bases:

>100_bases
CTCATCAGGAAATACAAGGAAAAAAATATCCATTCGATGATCGCGAGGTAGTGCGGCAGGTATTGCATGACCTGGCTGGT
CCAGAATTGCAGGTGCAGGG

Downstream 100 bases:

>100_bases
GAACAAGGTAGATCCTCTGATTAAAACTGTTTTTTGTGTCTGCGACCGGACATTGCAACGGGAACCGTAAAATGTTATTG
GCGCTTTTTCAATGGATTGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MMSVQEAALALHANSNWSGGNPIFTGVSTDSRTLRPGDLFIALSGEQFDGQHFISTAIEKGAVAAMVSTDTVIVPTKSDF
GWIKVNDTRLGFGQLAANWRQHFTLPLVAVTGSNGKTTVKEMIAAIFRHEFGSGNVLATTGNLNNDIGVPQMLLQLNTGH
TCAVIEMGMNHAGEIAYLSQLAAPTIAVITNAGTAHIEHLGTTEAIARAKGEIFTGLGAQGIAIINADDAYAQLWRQLAG
SRQILDFGITNPAAIRAQQMSSSSEAAWLLQLPDDKVEITLQVPGRHNIYNALAAAAAATAAGISTTSIAEGLHNFMGTP
GRLQKKVGLYQSILIDDTYNANPDSMQAALNVLAEMPGKKILIMGDMGELGTDAAGFHYTIGQQAAKAGIDALLALGELS
QQTVAGFGSGAQYFADLDTLLEKAKDCLGKNVSVLVKGSRFMRMERVIEQLQDRYVE

Sequences:

>Translated_457_residues
MMSVQEAALALHANSNWSGGNPIFTGVSTDSRTLRPGDLFIALSGEQFDGQHFISTAIEKGAVAAMVSTDTVIVPTKSDF
GWIKVNDTRLGFGQLAANWRQHFTLPLVAVTGSNGKTTVKEMIAAIFRHEFGSGNVLATTGNLNNDIGVPQMLLQLNTGH
TCAVIEMGMNHAGEIAYLSQLAAPTIAVITNAGTAHIEHLGTTEAIARAKGEIFTGLGAQGIAIINADDAYAQLWRQLAG
SRQILDFGITNPAAIRAQQMSSSSEAAWLLQLPDDKVEITLQVPGRHNIYNALAAAAAATAAGISTTSIAEGLHNFMGTP
GRLQKKVGLYQSILIDDTYNANPDSMQAALNVLAEMPGKKILIMGDMGELGTDAAGFHYTIGQQAAKAGIDALLALGELS
QQTVAGFGSGAQYFADLDTLLEKAKDCLGKNVSVLVKGSRFMRMERVIEQLQDRYVE
>Mature_457_residues
MMSVQEAALALHANSNWSGGNPIFTGVSTDSRTLRPGDLFIALSGEQFDGQHFISTAIEKGAVAAMVSTDTVIVPTKSDF
GWIKVNDTRLGFGQLAANWRQHFTLPLVAVTGSNGKTTVKEMIAAIFRHEFGSGNVLATTGNLNNDIGVPQMLLQLNTGH
TCAVIEMGMNHAGEIAYLSQLAAPTIAVITNAGTAHIEHLGTTEAIARAKGEIFTGLGAQGIAIINADDAYAQLWRQLAG
SRQILDFGITNPAAIRAQQMSSSSEAAWLLQLPDDKVEITLQVPGRHNIYNALAAAAAATAAGISTTSIAEGLHNFMGTP
GRLQKKVGLYQSILIDDTYNANPDSMQAALNVLAEMPGKKILIMGDMGELGTDAAGFHYTIGQQAAKAGIDALLALGELS
QQTVAGFGSGAQYFADLDTLLEKAKDCLGKNVSVLVKGSRFMRMERVIEQLQDRYVE

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=460, Percent_Identity=42.8260869565217, Blast_Score=309, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48445; Mature: 48445

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSVQEAALALHANSNWSGGNPIFTGVSTDSRTLRPGDLFIALSGEQFDGQHFISTAIEK
CCCHHCCCEEEECCCCCCCCCCEEEECCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHC
GAVAAMVSTDTVIVPTKSDFGWIKVNDTRLGFGQLAANWRQHFTLPLVAVTGSNGKTTVK
CCEEEEEECCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHH
EMIAAIFRHEFGSGNVLATTGNLNNDIGVPQMLLQLNTGHTCAVIEMGMNHAGEIAYLSQ
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHEEECCCCEEEEEEECCCCCCCHHHHHH
LAAPTIAVITNAGTAHIEHLGTTEAIARAKGEIFTGLGAQGIAIINADDAYAQLWRQLAG
HCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCEEECCCCCCEEEEECCHHHHHHHHHHCC
SRQILDFGITNPAAIRAQQMSSSSEAAWLLQLPDDKVEITLQVPGRHNIYNALAAAAAAT
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
AAGISTTSIAEGLHNFMGTPGRLQKKVGLYQSILIDDTYNANPDSMQAALNVLAEMPGKK
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCE
ILIMGDMGELGTDAAGFHYTIGQQAAKAGIDALLALGELSQQTVAGFGSGAQYFADLDTL
EEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LEKAKDCLGKNVSVLVKGSRFMRMERVIEQLQDRYVE
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSVQEAALALHANSNWSGGNPIFTGVSTDSRTLRPGDLFIALSGEQFDGQHFISTAIEK
CCCHHCCCEEEECCCCCCCCCCEEEECCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHC
GAVAAMVSTDTVIVPTKSDFGWIKVNDTRLGFGQLAANWRQHFTLPLVAVTGSNGKTTVK
CCEEEEEECCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHH
EMIAAIFRHEFGSGNVLATTGNLNNDIGVPQMLLQLNTGHTCAVIEMGMNHAGEIAYLSQ
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHEEECCCCEEEEEEECCCCCCCHHHHHH
LAAPTIAVITNAGTAHIEHLGTTEAIARAKGEIFTGLGAQGIAIINADDAYAQLWRQLAG
HCCCEEEEEECCCCHHHHHCCCHHHHHHHCCCEEECCCCCCEEEEECCHHHHHHHHHHCC
SRQILDFGITNPAAIRAQQMSSSSEAAWLLQLPDDKVEITLQVPGRHNIYNALAAAAAAT
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
AAGISTTSIAEGLHNFMGTPGRLQKKVGLYQSILIDDTYNANPDSMQAALNVLAEMPGKK
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCE
ILIMGDMGELGTDAAGFHYTIGQQAAKAGIDALLALGELSQQTVAGFGSGAQYFADLDTL
EEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LEKAKDCLGKNVSVLVKGSRFMRMERVIEQLQDRYVE
HHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]