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The map label for this gene is capI [H]

Identifier: 114330252

GI number: 114330252

Start: 238746

End: 239753

Strand: Direct

Name: capI [H]

Synonym: Neut_0225

Alternate gene names: 114330252

Gene position: 238746-239753 (Clockwise)

Preceding gene: 114330251

Following gene: 114330253

Centisome position: 8.97

GC content: 48.02

Gene sequence:

>1008_bases
TTGAAGATACTTATTACTGGTTCAGCCGGTTTTATTGGATCTGCATTGACGCTGCGGCTGCTTGAACGTGGTGACACCGT
CATTGGTATCGATAATCACAATGATTATTATGATCCACAGATTAAGGAGGATCGTCTTGCCCGGTTTGCGGATCATCCTG
CTTACACCCATTTGCGTCTTGACTTGGCAGATCGCGAAGGGATCAATTCCTGTTTTGAAATGCATAAACCACAACGGGTG
GTGAATCTGGCTGCTCAGGCTGGGGTGCGCTATTCCATTGAGAATCCATTGGCTTATATTGACAGCAATATCGTTGGTTT
TGCCCATATTCTTGAAGGTTGTCGTCATAACGGTGTCGAACATCTGGTGTATGCCAGCAGCTCTAGCGTGTACGGTGCCA
ATACGCAGATGCCGTTTTCGGTGCATCACAACGTTGATCACCCGCTCAGCCTTTATGCAGCCAGCAAAAAATCTAATGAG
CTAATGGCGCATACTTACAGCCATTTATATAACTTGTCGACTGCTGGCCTACGTTTTTTTACTGTATATGGTCCTTGGGG
GCGGCCGGATATGGCGCTGTTTAAATTCACTAAGGCTATTCTTGCCGGAGAAAAGATTCCGGTATTTAACTATGGAAAAC
ATCGCCGAGATTTTACTTATGTAGATGACATTGTGGAGGGCGTAATCCGTGTGCTGGATCAACCCACTCGGCCTGACCCG
GCCTGGAGCGGGGAAAATCCGGATGCAGGCACCAGTATGGCACCCTGGCGTGTTTACAACATTGGCAATAACAGCCCGGT
AGAGCTGATGGACTATATTGCTGCACTGGAAAAAGCGCTTGGTAAAAAGGCCGCAATGGAAATGCTACCCCTGCAGCCAG
GTGACGTACCGGATACTTATGCCGATGTTTCTGATCTCGTCGAACAATTCGATTACAGGCCCGCCACGTCGGTCGAACAG
GGAATAGCCAGCTTTGTGACGTGGTATCGCAATTACTTCAATTTGTAG

Upstream 100 bases:

>100_bases
GGGCTAAAGGAATTGCTGAGTTTTTCAACTTGCGGATCCCTTGTGTCAGGCAAGTTGCCACTTCAAGATTTATACACGTG
ATCTATTCGGGAGCAATTAA

Downstream 100 bases:

>100_bases
GAGACAACATGCAACTCCAGGATATCAAACTTGCCATTATCGGGCTTGGATATGTCGGCCTTCCACTGGCTGCTGAGTTT
GGCCGCAAGCGTCCTGTGAC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKILITGSAGFIGSALTLRLLERGDTVIGIDNHNDYYDPQIKEDRLARFADHPAYTHLRLDLADREGINSCFEMHKPQRV
VNLAAQAGVRYSIENPLAYIDSNIVGFAHILEGCRHNGVEHLVYASSSSVYGANTQMPFSVHHNVDHPLSLYAASKKSNE
LMAHTYSHLYNLSTAGLRFFTVYGPWGRPDMALFKFTKAILAGEKIPVFNYGKHRRDFTYVDDIVEGVIRVLDQPTRPDP
AWSGENPDAGTSMAPWRVYNIGNNSPVELMDYIAALEKALGKKAAMEMLPLQPGDVPDTYADVSDLVEQFDYRPATSVEQ
GIASFVTWYRNYFNL

Sequences:

>Translated_335_residues
MKILITGSAGFIGSALTLRLLERGDTVIGIDNHNDYYDPQIKEDRLARFADHPAYTHLRLDLADREGINSCFEMHKPQRV
VNLAAQAGVRYSIENPLAYIDSNIVGFAHILEGCRHNGVEHLVYASSSSVYGANTQMPFSVHHNVDHPLSLYAASKKSNE
LMAHTYSHLYNLSTAGLRFFTVYGPWGRPDMALFKFTKAILAGEKIPVFNYGKHRRDFTYVDDIVEGVIRVLDQPTRPDP
AWSGENPDAGTSMAPWRVYNIGNNSPVELMDYIAALEKALGKKAAMEMLPLQPGDVPDTYADVSDLVEQFDYRPATSVEQ
GIASFVTWYRNYFNL
>Mature_335_residues
MKILITGSAGFIGSALTLRLLERGDTVIGIDNHNDYYDPQIKEDRLARFADHPAYTHLRLDLADREGINSCFEMHKPQRV
VNLAAQAGVRYSIENPLAYIDSNIVGFAHILEGCRHNGVEHLVYASSSSVYGANTQMPFSVHHNVDHPLSLYAASKKSNE
LMAHTYSHLYNLSTAGLRFFTVYGPWGRPDMALFKFTKAILAGEKIPVFNYGKHRRDFTYVDDIVEGVIRVLDQPTRPDP
AWSGENPDAGTSMAPWRVYNIGNNSPVELMDYIAALEKALGKKAAMEMLPLQPGDVPDTYADVSDLVEQFDYRPATSVEQ
GIASFVTWYRNYFNL

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=26.4705882352941, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=25.8522727272727, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=25.8522727272727, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=25.8522727272727, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=26.2569832402235, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=26.7806267806268, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=23.8938053097345, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788589, Length=249, Percent_Identity=25.7028112449799, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=355, Percent_Identity=25.9154929577465, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=357, Percent_Identity=25.7703081232493, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=26.099706744868, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=339, Percent_Identity=24.4837758112094, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=351, Percent_Identity=26.2108262108262, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=26.7241379310345, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356223, Length=340, Percent_Identity=25, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37540; Mature: 37540

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILITGSAGFIGSALTLRLLERGDTVIGIDNHNDYYDPQIKEDRLARFADHPAYTHLRL
CEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEE
DLADREGINSCFEMHKPQRVVNLAAQAGVRYSIENPLAYIDSNIVGFAHILEGCRHNGVE
ECCCCHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCHHHHCCCHHHHHHHHHHHHHCCCE
HLVYASSSSVYGANTQMPFSVHHNVDHPLSLYAASKKSNELMAHTYSHLYNLSTAGLRFF
EEEEECCCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
TVYGPWGRPDMALFKFTKAILAGEKIPVFNYGKHRRDFTYVDDIVEGVIRVLDQPTRPDP
EEECCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AWSGENPDAGTSMAPWRVYNIGNNSPVELMDYIAALEKALGKKAAMEMLPLQPGDVPDTY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ADVSDLVEQFDYRPATSVEQGIASFVTWYRNYFNL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILITGSAGFIGSALTLRLLERGDTVIGIDNHNDYYDPQIKEDRLARFADHPAYTHLRL
CEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEEEE
DLADREGINSCFEMHKPQRVVNLAAQAGVRYSIENPLAYIDSNIVGFAHILEGCRHNGVE
ECCCCHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCHHHHCCCHHHHHHHHHHHHHCCCE
HLVYASSSSVYGANTQMPFSVHHNVDHPLSLYAASKKSNELMAHTYSHLYNLSTAGLRFF
EEEEECCCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE
TVYGPWGRPDMALFKFTKAILAGEKIPVFNYGKHRRDFTYVDDIVEGVIRVLDQPTRPDP
EEECCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AWSGENPDAGTSMAPWRVYNIGNNSPVELMDYIAALEKALGKKAAMEMLPLQPGDVPDTY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ADVSDLVEQFDYRPATSVEQGIASFVTWYRNYFNL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]