Click here to switch to the map view.

The map label for this gene is dacC [H]

Identifier: 114330251

GI number: 114330251

Start: 229758

End: 231185

Strand: Direct

Name: dacC [H]

Synonym: Neut_0223

Alternate gene names: 114330251

Gene position: 229758-231185 (Clockwise)

Preceding gene: 114330250

Following gene: 114330252

Centisome position: 8.63

GC content: 46.22

Gene sequence:

>1428_bases
ATGAAAAGTTTGCTGAAAATAACGTGGTTATTATGGGGTGCGTTGACTTTTCCGGCTTATGCCGTTGATTTGCCAGATAC
CGTCAGGCAGGCACTAAAAAAAACAGGCATTCCCGAAGCAGCTGTCGGGATTTATGTCAGGGAAGTCGGTGCTGAGCGGC
CGCTAGTGTCAGTCAATGCTGATGTGCCAATGAATCCTGCTTCAGTCATGAAGGTTGTAACAACGTATGCCGGACTGGAA
ATGCTTGGGCCATCATACACGTGGCGTACTAAGGTGTATGCAAATGGCAATCTGGAAAATGGCAGATTGCAAGGTGACTT
GATCATCAAGGGTTATGGCGATCCCAGTCTTAATCTGGAAAATTTCTGGCTGTTGCTCCGTCAGCTTCGCCAGTCCGGGC
TAAAAGATATCAGCGGGGATCTGGTACTGGATTACAGCTACTATAGTTTTCCGACGGAAAATCCTGGTGCTTTTGATGGT
AAACGCTATAAAACTTACAATGTGGCCCCGGAAGCGCTGCTGGTGAATTACCACACATCAACACTACATCTATTTCCCGA
ATCGCAGCATGGTGGAGTGCGGGTTACGGCCGATCCGGAAAGCCAATTGCTGAATGTGCAAAACCACCTCAAACTGACTC
AGAAAAAATGTAGCACCAGTGGAGTTCGCGTGAATATTCGTGACGATGTTCCGCGACCAGGCCATGTGACAGTAATGCTT
GAAGGTGATTACTCGGCTCATTGTGGCCAAACCGCTTACTACCTGAGTTTGCACGAGAGCTCGACGTACATTCATCAGCT
GTTTAGTGGGCTATGGAAGCAGCTGGATGGTACATTTAATGGCAGCGTACGGCATGGAGTAACATCAAAAACGCTGAATC
CGATTAGTGTCTACCATTCTCCGCCACTGGCCGAAGTCATTCGTGGTATTAACAAATTCAGTAATAACGTAGCAGCAAAA
CAGTTGTTTTTATCACTGGGTGAAACAGAATCTAACGGCGGCGAATTTGTTTCACCCAATCTTGCGCGATCTGGAATACG
ACAGTGGCTATTTTCAAAAAATATGGATTTTCCAGAATTGGTGCTGGAAAATGGATCGGGGTTGTCACGCATAGAACGAA
TCAGTGCGCGGCATTTAAATGACTTGCTGAATGCGGCTTATTTTTCGCCCACGATGCCTGAATTCATGGCCTCCCTGGCA
GTGGTCGGGGTGGATGGAACAGCTCGAAAGCGCTTAAAGAAATCCGTTGTTGCTCAAAAGGCGCATATTAAAACAGGCAC
ATTGAAAGATGTATCTGCGATAGCAGGGTATGTGCTGAACCATAAAAAAAAGCGTTATGTAATAGTATTCATCGTGAATC
ATCCGAAGTCAGGCGGTGCAAGAGTGGCTATGGATGCGCTATTAGAATGGTTGTATATAAAAACATAA

Upstream 100 bases:

>100_bases
ACGCTACGCGGCCGATATGCAGTACCGGATGGGTTGGGATGACGGGTTATCTATTTGTCGTGGTACACGTTGATCAAAAT
AAACTGATCCGCTGACTGTT

Downstream 100 bases:

>100_bases
AACATGAGGAAAGTGTAGATAGCGGGCTAGGGCCTGTTTCCGTTTGATCTCATTCAAGCTTTTGATTTAAAGAAGCAAGC
TCAATCGTTATAGCTCCCAC

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 4a; PBP-4a [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKSLLKITWLLWGALTFPAYAVDLPDTVRQALKKTGIPEAAVGIYVREVGAERPLVSVNADVPMNPASVMKVVTTYAGLE
MLGPSYTWRTKVYANGNLENGRLQGDLIIKGYGDPSLNLENFWLLLRQLRQSGLKDISGDLVLDYSYYSFPTENPGAFDG
KRYKTYNVAPEALLVNYHTSTLHLFPESQHGGVRVTADPESQLLNVQNHLKLTQKKCSTSGVRVNIRDDVPRPGHVTVML
EGDYSAHCGQTAYYLSLHESSTYIHQLFSGLWKQLDGTFNGSVRHGVTSKTLNPISVYHSPPLAEVIRGINKFSNNVAAK
QLFLSLGETESNGGEFVSPNLARSGIRQWLFSKNMDFPELVLENGSGLSRIERISARHLNDLLNAAYFSPTMPEFMASLA
VVGVDGTARKRLKKSVVAQKAHIKTGTLKDVSAIAGYVLNHKKKRYVIVFIVNHPKSGGARVAMDALLEWLYIKT

Sequences:

>Translated_475_residues
MKSLLKITWLLWGALTFPAYAVDLPDTVRQALKKTGIPEAAVGIYVREVGAERPLVSVNADVPMNPASVMKVVTTYAGLE
MLGPSYTWRTKVYANGNLENGRLQGDLIIKGYGDPSLNLENFWLLLRQLRQSGLKDISGDLVLDYSYYSFPTENPGAFDG
KRYKTYNVAPEALLVNYHTSTLHLFPESQHGGVRVTADPESQLLNVQNHLKLTQKKCSTSGVRVNIRDDVPRPGHVTVML
EGDYSAHCGQTAYYLSLHESSTYIHQLFSGLWKQLDGTFNGSVRHGVTSKTLNPISVYHSPPLAEVIRGINKFSNNVAAK
QLFLSLGETESNGGEFVSPNLARSGIRQWLFSKNMDFPELVLENGSGLSRIERISARHLNDLLNAAYFSPTMPEFMASLA
VVGVDGTARKRLKKSVVAQKAHIKTGTLKDVSAIAGYVLNHKKKRYVIVFIVNHPKSGGARVAMDALLEWLYIKT
>Mature_475_residues
MKSLLKITWLLWGALTFPAYAVDLPDTVRQALKKTGIPEAAVGIYVREVGAERPLVSVNADVPMNPASVMKVVTTYAGLE
MLGPSYTWRTKVYANGNLENGRLQGDLIIKGYGDPSLNLENFWLLLRQLRQSGLKDISGDLVLDYSYYSFPTENPGAFDG
KRYKTYNVAPEALLVNYHTSTLHLFPESQHGGVRVTADPESQLLNVQNHLKLTQKKCSTSGVRVNIRDDVPRPGHVTVML
EGDYSAHCGQTAYYLSLHESSTYIHQLFSGLWKQLDGTFNGSVRHGVTSKTLNPISVYHSPPLAEVIRGINKFSNNVAAK
QLFLSLGETESNGGEFVSPNLARSGIRQWLFSKNMDFPELVLENGSGLSRIERISARHLNDLLNAAYFSPTMPEFMASLA
VVGVDGTARKRLKKSVVAQKAHIKTGTLKDVSAIAGYVLNHKKKRYVIVFIVNHPKSGGARVAMDALLEWLYIKT

Specific function: Catalyzes DD-carboxypeptidase and transpeptidation reactions [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted (Probable). Note=May be anchored in the membrane via a C-terminal amphiphatic alpha helix [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=428, Percent_Identity=24.2990654205607, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52553; Mature: 52553

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLLKITWLLWGALTFPAYAVDLPDTVRQALKKTGIPEAAVGIYVREVGAERPLVSVNA
CCHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECC
DVPMNPASVMKVVTTYAGLEMLGPSYTWRTKVYANGNLENGRLQGDLIIKGYGDPSLNLE
CCCCCHHHHHHHHHHHHCHHHCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCCCCHH
NFWLLLRQLRQSGLKDISGDLVLDYSYYSFPTENPGAFDGKRYKTYNVAPEALLVNYHTS
HHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEECC
TLHLFPESQHGGVRVTADPESQLLNVQNHLKLTQKKCSTSGVRVNIRDDVPRPGHVTVML
EEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEE
EGDYSAHCGQTAYYLSLHESSTYIHQLFSGLWKQLDGTFNGSVRHGVTSKTLNPISVYHS
ECCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECC
PPLAEVIRGINKFSNNVAAKQLFLSLGETESNGGEFVSPNLARSGIRQWLFSKNMDFPEL
CCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
VLENGSGLSRIERISARHLNDLLNAAYFSPTMPEFMASLAVVGVDGTARKRLKKSVVAQK
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCHHHHHHHHHHHHHHH
AHIKTGTLKDVSAIAGYVLNHKKKRYVIVFIVNHPKSGGARVAMDALLEWLYIKT
HHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSLLKITWLLWGALTFPAYAVDLPDTVRQALKKTGIPEAAVGIYVREVGAERPLVSVNA
CCHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECC
DVPMNPASVMKVVTTYAGLEMLGPSYTWRTKVYANGNLENGRLQGDLIIKGYGDPSLNLE
CCCCCHHHHHHHHHHHHCHHHCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCCCCHH
NFWLLLRQLRQSGLKDISGDLVLDYSYYSFPTENPGAFDGKRYKTYNVAPEALLVNYHTS
HHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEECC
TLHLFPESQHGGVRVTADPESQLLNVQNHLKLTQKKCSTSGVRVNIRDDVPRPGHVTVML
EEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEE
EGDYSAHCGQTAYYLSLHESSTYIHQLFSGLWKQLDGTFNGSVRHGVTSKTLNPISVYHS
ECCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECC
PPLAEVIRGINKFSNNVAAKQLFLSLGETESNGGEFVSPNLARSGIRQWLFSKNMDFPEL
CCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
VLENGSGLSRIERISARHLNDLLNAAYFSPTMPEFMASLAVVGVDGTARKRLKKSVVAQK
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCHHHHHHHHHHHHHHH
AHIKTGTLKDVSAIAGYVLNHKKKRYVIVFIVNHPKSGGARVAMDALLEWLYIKT
HHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377 [H]