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The map label for this gene is kinB [H]

Identifier: 113969679

GI number: 113969679

Start: 1549812

End: 1551173

Strand: Direct

Name: kinB [H]

Synonym: Shewmr4_1337

Alternate gene names: 113969679

Gene position: 1549812-1551173 (Clockwise)

Preceding gene: 113969676

Following gene: 113969680

Centisome position: 32.93

GC content: 43.61

Gene sequence:

>1362_bases
ATGACACGTATGTTAGACAGGATTTTATTCGATGTAAGTCGTTCATCCATCATTGATGGTGGCGATCTTATTGAGGCCTC
ACAACTTATCGTGTCATCTGTGTGCCAAGGGTTAAAAATCACCCGTGCTGGTATTTGGTTATGGAGTGAAGATCACCAAA
GTATGCATTGCTTTTACCTGCTCGATAATATGCACAGGCAAGAGGCGTTGATATTAACCCGGCAGGATTATCCACATTAT
TTCAGTCTATTAGATAATGAGCGCGCTATTGTCGCGAATCATGCGCAAACTGATGGGGCGACCCATGAGTTTGTTAGTGC
CTACTTAGTCCCTAATCGTATTAGTTCTCTGTTAGATAGTCCCATACGTCATCGCGGAAAAATGGTCGGCATTATCTGCT
CCGAGCATCAAGGTGTGGCTCGAGAATGGACGCGAGATGAAAGGGCCTTTGCTTCAGCGCTGGCCGATTTATTCGGCAGA
GCCATGAGCGCAAAAGAGATTTTGGATTATCAGCATCAACTAAAAGCTATTAATGCGGATTTGGAAGTTCAGGTTGAACA
GCGAACGCAAGAACTTGAAACCGCATTATCGGATTTGCGCTCAATACAACACCATCTAGTTGAAAACGAAAAAATGGCGG
CCTTAGGGAGTTTGGTCGCAGGGGTTGCTCACGAGGTCAATACGCCTCTCGGGATTGCCGTGACATCCGTGACCCATTGT
TTAGATGAAGTGGCATTATTGAAAACGGCTTTCGAGCAAAATGATCTCGATGAGGATAGATTTTTATCCTTTATCAATAC
CATGCAGGATGGTTTAGGGTTGATTGAGCGCAATCTTTCGCGAGCAGCTGAGTTAGTCCATAACTTTAAGCGCACCGCCG
CAGACCAATCGGTGCTAGAACGTGAGCGGTTTAATTTAAAGACCTACATTTTTCAAATTTTCAGCTCATTAAGGCCGTTA
ATGCGTAAGAAGAATATTGCCTTAAATGTGGAGCTTGATGACGACATCTTTATCGAATCTTACCCTGGTGCCATCGCACA
GATTTTCACTAATTTAGTGGCTAATAGCTTTCGCCATGGATTTCCTGAGAGTTTTACGGGGGATAAAATCATCACTATTC
GAGTACAAAAACAAGACTCGAATATTTGTATGCAGTATCAAGATAATGGGGTAGGGATGACCGATGAGGTTAAACTGAAA
GCCTTCGAGCCTTTCTTCACTACCGCACGTAAAGACGGCGGCACAGGTCTTGGCATGTCGATTATTTATAATTTAGTGAC
GCAAAAACTTCATGGCACCATTATGCTGACCTCATCTCCATATCAAGGTGTGAAAGTTGAAATACAGATCCCTGAGAAAT
GA

Upstream 100 bases:

>100_bases
CAGATTGAATCTATACTAAAGAGAATCACTTAAGCTTGGGTCACCAAGTTCATTGCCTCATTTTTAAGTCAGGCGGTACC
ACGCGGGGTAAATCTGCATT

Downstream 100 bases:

>100_bases
CCTAAGCACATTGTTTAGGCGGTAATACGGCCTTGTTTGAGACCTCGCGCCATTCAGCTTTATCGGCATTTATATGTTAC
AATCATCCCTTCACTTTGAC

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTRMLDRILFDVSRSSIIDGGDLIEASQLIVSSVCQGLKITRAGIWLWSEDHQSMHCFYLLDNMHRQEALILTRQDYPHY
FSLLDNERAIVANHAQTDGATHEFVSAYLVPNRISSLLDSPIRHRGKMVGIICSEHQGVAREWTRDERAFASALADLFGR
AMSAKEILDYQHQLKAINADLEVQVEQRTQELETALSDLRSIQHHLVENEKMAALGSLVAGVAHEVNTPLGIAVTSVTHC
LDEVALLKTAFEQNDLDEDRFLSFINTMQDGLGLIERNLSRAAELVHNFKRTAADQSVLERERFNLKTYIFQIFSSLRPL
MRKKNIALNVELDDDIFIESYPGAIAQIFTNLVANSFRHGFPESFTGDKIITIRVQKQDSNICMQYQDNGVGMTDEVKLK
AFEPFFTTARKDGGTGLGMSIIYNLVTQKLHGTIMLTSSPYQGVKVEIQIPEK

Sequences:

>Translated_453_residues
MTRMLDRILFDVSRSSIIDGGDLIEASQLIVSSVCQGLKITRAGIWLWSEDHQSMHCFYLLDNMHRQEALILTRQDYPHY
FSLLDNERAIVANHAQTDGATHEFVSAYLVPNRISSLLDSPIRHRGKMVGIICSEHQGVAREWTRDERAFASALADLFGR
AMSAKEILDYQHQLKAINADLEVQVEQRTQELETALSDLRSIQHHLVENEKMAALGSLVAGVAHEVNTPLGIAVTSVTHC
LDEVALLKTAFEQNDLDEDRFLSFINTMQDGLGLIERNLSRAAELVHNFKRTAADQSVLERERFNLKTYIFQIFSSLRPL
MRKKNIALNVELDDDIFIESYPGAIAQIFTNLVANSFRHGFPESFTGDKIITIRVQKQDSNICMQYQDNGVGMTDEVKLK
AFEPFFTTARKDGGTGLGMSIIYNLVTQKLHGTIMLTSSPYQGVKVEIQIPEK
>Mature_452_residues
TRMLDRILFDVSRSSIIDGGDLIEASQLIVSSVCQGLKITRAGIWLWSEDHQSMHCFYLLDNMHRQEALILTRQDYPHYF
SLLDNERAIVANHAQTDGATHEFVSAYLVPNRISSLLDSPIRHRGKMVGIICSEHQGVAREWTRDERAFASALADLFGRA
MSAKEILDYQHQLKAINADLEVQVEQRTQELETALSDLRSIQHHLVENEKMAALGSLVAGVAHEVNTPLGIAVTSVTHCL
DEVALLKTAFEQNDLDEDRFLSFINTMQDGLGLIERNLSRAAELVHNFKRTAADQSVLERERFNLKTYIFQIFSSLRPLM
RKKNIALNVELDDDIFIESYPGAIAQIFTNLVANSFRHGFPESFTGDKIITIRVQKQDSNICMQYQDNGVGMTDEVKLKA
FEPFFTTARKDGGTGLGMSIIYNLVTQKLHGTIMLTSSPYQGVKVEIQIPEK

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the kinB activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=276, Percent_Identity=23.5507246376812, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=298, Percent_Identity=22.1476510067114, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=292, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081816, Length=303, Percent_Identity=25.0825082508251, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790436, Length=251, Percent_Identity=23.9043824701195, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51090; Mature: 50959

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRMLDRILFDVSRSSIIDGGDLIEASQLIVSSVCQGLKITRAGIWLWSEDHQSMHCFYL
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEE
LDNMHRQEALILTRQDYPHYFSLLDNERAIVANHAQTDGATHEFVSAYLVPNRISSLLDS
EHHCCCCCEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
PIRHRGKMVGIICSEHQGVAREWTRDERAFASALADLFGRAMSAKEILDYQHQLKAINAD
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
LEVQVEQRTQELETALSDLRSIQHHLVENEKMAALGSLVAGVAHEVNTPLGIAVTSVTHC
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LDEVALLKTAFEQNDLDEDRFLSFINTMQDGLGLIERNLSRAAELVHNFKRTAADQSVLE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERFNLKTYIFQIFSSLRPLMRKKNIALNVELDDDIFIESYPGAIAQIFTNLVANSFRHG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCC
FPESFTGDKIITIRVQKQDSNICMQYQDNGVGMTDEVKLKAFEPFFTTARKDGGTGLGMS
CCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECHHHHHHCCCCCCCHHHH
IIYNLVTQKLHGTIMLTSSPYQGVKVEIQIPEK
HHHHHHHHHHCCEEEEECCCCCCEEEEEECCCC
>Mature Secondary Structure 
TRMLDRILFDVSRSSIIDGGDLIEASQLIVSSVCQGLKITRAGIWLWSEDHQSMHCFYL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEE
LDNMHRQEALILTRQDYPHYFSLLDNERAIVANHAQTDGATHEFVSAYLVPNRISSLLDS
EHHCCCCCEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
PIRHRGKMVGIICSEHQGVAREWTRDERAFASALADLFGRAMSAKEILDYQHQLKAINAD
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
LEVQVEQRTQELETALSDLRSIQHHLVENEKMAALGSLVAGVAHEVNTPLGIAVTSVTHC
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LDEVALLKTAFEQNDLDEDRFLSFINTMQDGLGLIERNLSRAAELVHNFKRTAADQSVLE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERFNLKTYIFQIFSSLRPLMRKKNIALNVELDDDIFIESYPGAIAQIFTNLVANSFRHG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCC
FPESFTGDKIITIRVQKQDSNICMQYQDNGVGMTDEVKLKAFEPFFTTARKDGGTGLGMS
CCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEECHHHHHHCCCCCCCHHHH
IIYNLVTQKLHGTIMLTSSPYQGVKVEIQIPEK
HHHHHHHHHHCCEEEEECCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]