Click here to switch to the map view.

The map label for this gene is braB [H]

Identifier: 113969138

GI number: 113969138

Start: 928383

End: 929786

Strand: Direct

Name: braB [H]

Synonym: Shewmr4_0794

Alternate gene names: 113969138

Gene position: 928383-929786 (Clockwise)

Preceding gene: 113969136

Following gene: 113969139

Centisome position: 19.73

GC content: 49.0

Gene sequence:

>1404_bases
GTGCAAGACAATCAGATGAGTATCGGGGATACATTAGGCTTAGGTTTTATGACCTTTGCATTTTTTTTGGGGGCGGGTAA
CTTAATTTTTCCACCCTTTGCGGGTATGTTGGCCGGGGAGAATATGTTCCTTGCCATGTTAGGTTTTCTCATCACTGCCG
TAGGTTTACCTTTAGTCGGGCTCATTGCTGTTGCTAAGGCTCAAGGTAAGGTTATGGCAATGCTGCCAGTGTTTGCGGCA
ACCGCATTGGCCATCGCGATTTATATCATTATTGGGCCCGCGTTTGCGGCGCCACGTACCGGCCTTGTGGCCTATGAAAT
CGGTGCAAAGCCCTTTATCGATAACACGACTGCGACCATTATGCTCGGCAATTTATCGCTTAATTTGTCGCAGTTAATCT
ATACCCTGTGCTTCTTTACCGTGACTATGCTACTGGCATTGTTCCCCGGTAAATTACTCGACAGCGTCGGTAAAGTATTA
ACGCCTATCCTATTGTTATTGCTGGTAGGTTTAGCCTTATCTGTGGTGTTTTTACCCGCAGCAGATGTAGGTGTGGCCGT
GGGTGACTACCAAAACCATCCGCTGACCAAAGGTATCCTCGAAGGCTACAACACCATGGATACCCTAGCGTCGTTGATGT
TTGGTATGTTGATTATCGATCTGCTGCGTAAAAAGGGGATCGACCAACCTAGCGCGCAGACTAAATACTTGATCCGTGCA
GCCTTTATTGCGGCGGCAGGTCTGGCATTTGTTTATGTGTCTCTGTTCTTTTTAGGTGCGACGGCGGGTGATATTGCCGT
AGGTGCTAAAAACGGTGGCGAGATTTTAACTAACTATGTGACACGTGAGTTTGGCAGTTTAGGCGCGGTATTACTGTCTA
CCGTTGTGACCTTAGCCTGTTTAACCACGGCGGTCGGTTTAGTGAGTGCCTGCTCAGAGTTTTTCAATGAGTTGATGCCA
AAGCTGTCTTACCGTTTCTTGGTGGTTGTACTCAGCGCTATCTGTGCCTTGATTGCGAACGTGGGGCTGTCTCAGCTGAT
CGCTATCAGTATTCCTGTGTTGATGGCAGTTTACCCTGTGGCTATTACCTTAGTGGCGGTAACTTTCTTGACCGAATACT
TCCCGCGTCCACAGTTTGCTCACCGTGCTGCACTGTCTGTGGCACTGCTGTTTGGTATTTTCGATGGGATGAAAGTCGCG
GCGAAAGGCCTCACAGGTGAAGACGGTAAAGGCATCACCGAAGCGGCGCAGAGCTTTATCGATATGGTGAGTGGTATGTC
TCCCACACTGTCGATTTTACCTCTGTATGAAGAGGGGATGGCGTGGTTACTGCCAACCTTAGTCGTGATCGTGCTGTGTT
TATTGTTTGGCCGCGGTGGTGCGAAAGCGGTCAGCGCGGCATAA

Upstream 100 bases:

>100_bases
ACATAGATTTGCACTCCGGATGGGCTTTGTTTTAGCTATGGCTAATCGACACATAATCACTTAAGTTTATTAAAAACAAT
AAGACAAGATGGGGCTTTCC

Downstream 100 bases:

>100_bases
ACAAGACGCTGCCACAAGCAGAACAATTGAGTTAATATCAACGGCGTATCCTTAGAATACGCCGTTTTTTTATGGAGTTT
TTGTGGCTAAAACCTATCAG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MQDNQMSIGDTLGLGFMTFAFFLGAGNLIFPPFAGMLAGENMFLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPVFAA
TALAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDNTTATIMLGNLSLNLSQLIYTLCFFTVTMLLALFPGKLLDSVGKVL
TPILLLLLVGLALSVVFLPAADVGVAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDLLRKKGIDQPSAQTKYLIRA
AFIAAAGLAFVYVSLFFLGATAGDIAVGAKNGGEILTNYVTREFGSLGAVLLSTVVTLACLTTAVGLVSACSEFFNELMP
KLSYRFLVVVLSAICALIANVGLSQLIAISIPVLMAVYPVAITLVAVTFLTEYFPRPQFAHRAALSVALLFGIFDGMKVA
AKGLTGEDGKGITEAAQSFIDMVSGMSPTLSILPLYEEGMAWLLPTLVVIVLCLLFGRGGAKAVSAA

Sequences:

>Translated_467_residues
MQDNQMSIGDTLGLGFMTFAFFLGAGNLIFPPFAGMLAGENMFLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPVFAA
TALAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDNTTATIMLGNLSLNLSQLIYTLCFFTVTMLLALFPGKLLDSVGKVL
TPILLLLLVGLALSVVFLPAADVGVAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDLLRKKGIDQPSAQTKYLIRA
AFIAAAGLAFVYVSLFFLGATAGDIAVGAKNGGEILTNYVTREFGSLGAVLLSTVVTLACLTTAVGLVSACSEFFNELMP
KLSYRFLVVVLSAICALIANVGLSQLIAISIPVLMAVYPVAITLVAVTFLTEYFPRPQFAHRAALSVALLFGIFDGMKVA
AKGLTGEDGKGITEAAQSFIDMVSGMSPTLSILPLYEEGMAWLLPTLVVIVLCLLFGRGGAKAVSAA
>Mature_467_residues
MQDNQMSIGDTLGLGFMTFAFFLGAGNLIFPPFAGMLAGENMFLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPVFAA
TALAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDNTTATIMLGNLSLNLSQLIYTLCFFTVTMLLALFPGKLLDSVGKVL
TPILLLLLVGLALSVVFLPAADVGVAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDLLRKKGIDQPSAQTKYLIRA
AFIAAAGLAFVYVSLFFLGATAGDIAVGAKNGGEILTNYVTREFGSLGAVLLSTVVTLACLTTAVGLVSACSEFFNELMP
KLSYRFLVVVLSAICALIANVGLSQLIAISIPVLMAVYPVAITLVAVTFLTEYFPRPQFAHRAALSVALLFGIFDGMKVA
AKGLTGEDGKGITEAAQSFIDMVSGMSPTLSILPLYEEGMAWLLPTLVVIVLCLLFGRGGAKAVSAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=467, Percent_Identity=38.7580299785867, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48928; Mature: 48928

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNQMSIGDTLGLGFMTFAFFLGAGNLIFPPFAGMLAGENMFLAMLGFLITAVGLPLVG
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LIAVAKAQGKVMAMLPVFAATALAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDNTTATI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCCCCCEEEE
MLGNLSLNLSQLIYTLCFFTVTMLLALFPGKLLDSVGKVLTPILLLLLVGLALSVVFLPA
EEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADVGVAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDLLRKKGIDQPSAQTKYLIRA
HHHCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AFIAAAGLAFVYVSLFFLGATAGDIAVGAKNGGEILTNYVTREFGSLGAVLLSTVVTLAC
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTAVGLVSACSEFFNELMPKLSYRFLVVVLSAICALIANVGLSQLIAISIPVLMAVYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AITLVAVTFLTEYFPRPQFAHRAALSVALLFGIFDGMKVAAKGLTGEDGKGITEAAQSFI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
DMVSGMSPTLSILPLYEEGMAWLLPTLVVIVLCLLFGRGGAKAVSAA
HHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQDNQMSIGDTLGLGFMTFAFFLGAGNLIFPPFAGMLAGENMFLAMLGFLITAVGLPLVG
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LIAVAKAQGKVMAMLPVFAATALAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDNTTATI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCCCCCEEEE
MLGNLSLNLSQLIYTLCFFTVTMLLALFPGKLLDSVGKVLTPILLLLLVGLALSVVFLPA
EEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADVGVAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDLLRKKGIDQPSAQTKYLIRA
HHHCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AFIAAAGLAFVYVSLFFLGATAGDIAVGAKNGGEILTNYVTREFGSLGAVLLSTVVTLAC
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTAVGLVSACSEFFNELMPKLSYRFLVVVLSAICALIANVGLSQLIAISIPVLMAVYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AITLVAVTFLTEYFPRPQFAHRAALSVALLFGIFDGMKVAAKGLTGEDGKGITEAAQSFI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
DMVSGMSPTLSILPLYEEGMAWLLPTLVVIVLCLLFGRGGAKAVSAA
HHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]