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The map label for this gene is rpfG [H]

Identifier: 113969136

GI number: 113969136

Start: 926064

End: 927059

Strand: Direct

Name: rpfG [H]

Synonym: Shewmr4_0792

Alternate gene names: 113969136

Gene position: 926064-927059 (Clockwise)

Preceding gene: 113969135

Following gene: 113969138

Centisome position: 19.68

GC content: 47.09

Gene sequence:

>996_bases
ATGCAGTCTGCATCTGAACATAAAAAAACCTTATTGTTGGTGGATGATGAGCCGGTTAACTTACGGGTGCTCAAGCAAGT
GTTGCATCAGGATTATCATCTTATCTTTGCCAAGAGTGGCGAAGAGGCCTTGCGTTTAGCACAAACTGAACTGCCATCGC
TGATCCTACTCGACATCATGATGCCCAATATGACGGGACTGGAAGTCTGTCAGTTGCTCAAAAATATCCCAGAGACTCAG
TCTATCCCGGTGATTTTTGTCACCGCATTGAATGATGAGCACGACGAAGCAGCAGGCTTTGCCGTGGGCGGAGTGGATTA
CATCGTCAAGCCGATATCGGCCACCATAGTCAAAGCACGGGTGAAAACCCATTTATCCTTAGTGCAGGCCGATGAATTGC
GTCGCACACGCTTGCAAGTGATCCAACGCTTGGGCCGCGCCGCTGAATATAAAGATAACGAAACTGGCACCCATGTGCTG
CGCATGAGCCATTATTCTAAAATTTTGGCATTAGCCTATGGACTGAGTGAGGCACAGGCGGATGATTTATTGCACGCGGC
GCCCATGCACGATATAGGTAAAATCGGTATTCCCGATAGCATTATGCTTAAACCGGGTAAGTTAACCGAAGAAGAATTCG
CCATTATGAAAAACCATCCCCATATTGGCGCCGAAATTATTGGTGAGGCCGACTCTGAGCTGCTGCGGCTAGCCAAATCC
GTGTCCCTAACCCACCATGAAAAATGGAACGGCACTGGTTACCCTAATGGGCTGAAAGGAGAGGAAATTCCGTTAGAGGG
AAGGATAGTCGCCTTGGCCGATGTATTTGATGCACTGACCAGCAAACGTCCCTATAAAGAAGCATGGTCAATTGAGAAGA
CCATGGACTATATCCAGGAGCAGAGTGGTGTACATTTCGACCCTGAGTTGGTGCGCTTATTTACCGAGAACTTAGATAAA
GTGATTGAAATTAAAAATAAATGGATAGATAGCTGA

Upstream 100 bases:

>100_bases
TGACGATAATTACCGAGGCCTTAGATAACTTTGATTTTGATATTGCCCATAACGAATTGCTCGAGTTACGGCGTATTATC
ACCGCGAATCAGGAGTAGCA

Downstream 100 bases:

>100_bases
TGTTGTAACAAGTCAATCACGTTCGTTAGTCGCTATGGATAAGGCTCTCTTTGGAGCCTTATCATTCAAACGAGTCAAAG
CTTAAGATAAAAATCCTTGG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQSASEHKKTLLLVDDEPVNLRVLKQVLHQDYHLIFAKSGEEALRLAQTELPSLILLDIMMPNMTGLEVCQLLKNIPETQ
SIPVIFVTALNDEHDEAAGFAVGGVDYIVKPISATIVKARVKTHLSLVQADELRRTRLQVIQRLGRAAEYKDNETGTHVL
RMSHYSKILALAYGLSEAQADDLLHAAPMHDIGKIGIPDSIMLKPGKLTEEEFAIMKNHPHIGAEIIGEADSELLRLAKS
VSLTHHEKWNGTGYPNGLKGEEIPLEGRIVALADVFDALTSKRPYKEAWSIEKTMDYIQEQSGVHFDPELVRLFTENLDK
VIEIKNKWIDS

Sequences:

>Translated_331_residues
MQSASEHKKTLLLVDDEPVNLRVLKQVLHQDYHLIFAKSGEEALRLAQTELPSLILLDIMMPNMTGLEVCQLLKNIPETQ
SIPVIFVTALNDEHDEAAGFAVGGVDYIVKPISATIVKARVKTHLSLVQADELRRTRLQVIQRLGRAAEYKDNETGTHVL
RMSHYSKILALAYGLSEAQADDLLHAAPMHDIGKIGIPDSIMLKPGKLTEEEFAIMKNHPHIGAEIIGEADSELLRLAKS
VSLTHHEKWNGTGYPNGLKGEEIPLEGRIVALADVFDALTSKRPYKEAWSIEKTMDYIQEQSGVHFDPELVRLFTENLDK
VIEIKNKWIDS
>Mature_331_residues
MQSASEHKKTLLLVDDEPVNLRVLKQVLHQDYHLIFAKSGEEALRLAQTELPSLILLDIMMPNMTGLEVCQLLKNIPETQ
SIPVIFVTALNDEHDEAAGFAVGGVDYIVKPISATIVKARVKTHLSLVQADELRRTRLQVIQRLGRAAEYKDNETGTHVL
RMSHYSKILALAYGLSEAQADDLLHAAPMHDIGKIGIPDSIMLKPGKLTEEEFAIMKNHPHIGAEIIGEADSELLRLAKS
VSLTHHEKWNGTGYPNGLKGEEIPLEGRIVALADVFDALTSKRPYKEAWSIEKTMDYIQEQSGVHFDPELVRLFTENLDK
VIEIKNKWIDS

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=121, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=34.4, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=104, Percent_Identity=36.5384615384615, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=31.304347826087, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=36.2068965517241, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37031; Mature: 37031

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSASEHKKTLLLVDDEPVNLRVLKQVLHQDYHLIFAKSGEEALRLAQTELPSLILLDIM
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCHHHHHHHH
MPNMTGLEVCQLLKNIPETQSIPVIFVTALNDEHDEAAGFAVGGVDYIVKPISATIVKAR
CCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCEEECCHHHHHHHHHHHHHHHH
VKTHLSLVQADELRRTRLQVIQRLGRAAEYKDNETGTHVLRMSHYSKILALAYGLSEAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHHHHCCCHHHH
DDLLHAAPMHDIGKIGIPDSIMLKPGKLTEEEFAIMKNHPHIGAEIIGEADSELLRLAKS
HHHHHHCCCHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHH
VSLTHHEKWNGTGYPNGLKGEEIPLEGRIVALADVFDALTSKRPYKEAWSIEKTMDYIQE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QSGVHFDPELVRLFTENLDKVIEIKNKWIDS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSASEHKKTLLLVDDEPVNLRVLKQVLHQDYHLIFAKSGEEALRLAQTELPSLILLDIM
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCHHHHHHHH
MPNMTGLEVCQLLKNIPETQSIPVIFVTALNDEHDEAAGFAVGGVDYIVKPISATIVKAR
CCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCEEECCHHHHHHHHHHHHHHHH
VKTHLSLVQADELRRTRLQVIQRLGRAAEYKDNETGTHVLRMSHYSKILALAYGLSEAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHHHHCCCHHHH
DDLLHAAPMHDIGKIGIPDSIMLKPGKLTEEEFAIMKNHPHIGAEIIGEADSELLRLAKS
HHHHHHCCCHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHH
VSLTHHEKWNGTGYPNGLKGEEIPLEGRIVALADVFDALTSKRPYKEAWSIEKTMDYIQE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QSGVHFDPELVRLFTENLDKVIEIKNKWIDS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]