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The map label for this gene is pucG [H]

Identifier: 113968710

GI number: 113968710

Start: 392794

End: 393927

Strand: Direct

Name: pucG [H]

Synonym: Shewmr4_0366

Alternate gene names: 113968710

Gene position: 392794-393927 (Clockwise)

Preceding gene: 113968709

Following gene: 113968711

Centisome position: 8.35

GC content: 52.03

Gene sequence:

>1134_bases
ATGTTACCTGCACCGCATTTTTCTGCCTTTAATCCGCCTCGCCGCATTTTGATGGGGCCAGGTCCATCGGATGTTTATCC
TGAAGTATTAGCCGCACAGGCAAGACCGACCGTCGGCCACCTAGATCCATTATTTGTTGGCATGATGGATGAGCTTAAGA
GCCTGATCCAATATGCGTTTCAAACCAAAAATGAAATGACGATGGCAGTGTCGGCCCCCGGCAGTGCAGGCATGGAAACC
TGTTTTGTGAACTTAGTCGAACCCGGCGAAAAGGTGATTGTGTGCCGCAATGGCGTGTTTGGTGAGCGTATGCGCCAAAA
CGTCGAGCGTGTAGGTGCGATTGCTGTGCTGGTGGATAATGAATGGGGCACACCCGTCGACCCTGCTGCCGTTGAAGCGG
CGCTTAAGGCCAACCCCGATGCTAAATTCCTTGCCTTTGTGCACGCCGAAACTTCAACGGGCGCATTGAGCGATGCTAAA
ATCCTGTGCGCGCTGGCAAAACAATACGGCTGTTTATCGATTGTCGATGCGGTGACATCCCTCGGTGGCGTGGAATTAAG
AGTCGATGAATGGGGAATCGATGCCATTTATTCTGGTAGTCAGAAATGTCTCTCCTGCGTGCCGGGATTATCGCCTGTGT
CTTTCTCGCCCAATGCGGTGGAAAAGCTTAAAAACCGTAAAACGCCAGTGCAGAGCTGGTTCCTCGATCAAAGTCTGGTC
ATGGCCTATTGGACTAGCGCTGGTGGCAAGCGCAGTTATCACCATACCGCGCCCGTTAATGCTCTTTATGCCCTGCACGA
ATCGCTGCGTTTATTGGCCGAAGAAGGTTTAGAAAATGCGTGGAAGCGTCATCAAGACATGCATTTAGTGCTGCGCGCCG
GCCTTGAAAAACTTGGCCTGAAATTTGTGGTTGCTGAAGCTTCGTGCTTGCCGCAGCTAAATGCCATTTATATCCCCAAA
GGCGTTGATGATGCCGCCGTGCGTGCGCGTCTACTCAAGGATTACAACCTTGAAATTGGTGCTGGTCTTGGTGCTCTAGC
GGGTAAAGCTTGGCGCATTGGTTTAATGGGCTTTGGTGCCCGCCGTGAGAATGTCGCGCTCTGTTTGCGCGCGCTAGAAG
AGGTGCTGAACTAA

Upstream 100 bases:

>100_bases
ACAGGCGCCCCTTTTTGTGGTAGTTTGGTTTAACAAGTTAAGAGTGAATGCTCTAGTCACACAATAATCAGACCTCGACC
CTACGCATTAAGGAGACATG

Downstream 100 bases:

>100_bases
TATGTCAGTTTCGCGTATAAGGATGTTACGCAGAGTGAGTTTGCGCTTAGTGAGTTCACGTTTATTGATTGGCGGTTTAA
TTCTGATCTTAAGTGGTTGT

Product: alanine-glyoxylate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLPAPHFSAFNPPRRILMGPGPSDVYPEVLAAQARPTVGHLDPLFVGMMDELKSLIQYAFQTKNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDNEWGTPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAK
ILCALAKQYGCLSIVDAVTSLGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPNAVEKLKNRKTPVQSWFLDQSLV
MAYWTSAGGKRSYHHTAPVNALYALHESLRLLAEEGLENAWKRHQDMHLVLRAGLEKLGLKFVVAEASCLPQLNAIYIPK
GVDDAAVRARLLKDYNLEIGAGLGALAGKAWRIGLMGFGARRENVALCLRALEEVLN

Sequences:

>Translated_377_residues
MLPAPHFSAFNPPRRILMGPGPSDVYPEVLAAQARPTVGHLDPLFVGMMDELKSLIQYAFQTKNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDNEWGTPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAK
ILCALAKQYGCLSIVDAVTSLGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPNAVEKLKNRKTPVQSWFLDQSLV
MAYWTSAGGKRSYHHTAPVNALYALHESLRLLAEEGLENAWKRHQDMHLVLRAGLEKLGLKFVVAEASCLPQLNAIYIPK
GVDDAAVRARLLKDYNLEIGAGLGALAGKAWRIGLMGFGARRENVALCLRALEEVLN
>Mature_377_residues
MLPAPHFSAFNPPRRILMGPGPSDVYPEVLAAQARPTVGHLDPLFVGMMDELKSLIQYAFQTKNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDNEWGTPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAK
ILCALAKQYGCLSIVDAVTSLGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPNAVEKLKNRKTPVQSWFLDQSLV
MAYWTSAGGKRSYHHTAPVNALYALHESLRLLAEEGLENAWKRHQDMHLVLRAGLEKLGLKFVVAEASCLPQLNAIYIPK
GVDDAAVRARLLKDYNLEIGAGLGALAGKAWRIGLMGFGARRENVALCLRALEEVLN

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=366, Percent_Identity=39.0710382513661, Blast_Score=261, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17536281, Length=357, Percent_Identity=39.4957983193277, Blast_Score=248, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321079, Length=371, Percent_Identity=27.7628032345013, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17530823, Length=368, Percent_Identity=38.8586956521739, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 40739; Mature: 40739

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAPHFSAFNPPRRILMGPGPSDVYPEVLAAQARPTVGHLDPLFVGMMDELKSLIQYAF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QTKNEMTMAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDN
HCCCCEEEEEECCCCCCHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEEC
EWGTPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAKILCALAKQYGCLSIVDAVTS
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPNAVEKLKNRKTPVQSWFLDQSLV
CCCEEEEEECCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
MAYWTSAGGKRSYHHTAPVNALYALHESLRLLAEEGLENAWKRHQDMHLVLRAGLEKLGL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCE
KFVVAEASCLPQLNAIYIPKGVDDAAVRARLLKDYNLEIGAGLGALAGKAWRIGLMGFGA
EEEEECHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCEECCCHHHHCCCCEEEEEEECCC
RRENVALCLRALEEVLN
CHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLPAPHFSAFNPPRRILMGPGPSDVYPEVLAAQARPTVGHLDPLFVGMMDELKSLIQYAF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QTKNEMTMAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDN
HCCCCEEEEEECCCCCCHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEEC
EWGTPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAKILCALAKQYGCLSIVDAVTS
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSPNAVEKLKNRKTPVQSWFLDQSLV
CCCEEEEEECCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
MAYWTSAGGKRSYHHTAPVNALYALHESLRLLAEEGLENAWKRHQDMHLVLRAGLEKLGL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCE
KFVVAEASCLPQLNAIYIPKGVDDAAVRARLLKDYNLEIGAGLGALAGKAWRIGLMGFGA
EEEEECHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCEECCCHHHHCCCCEEEEEEECCC
RRENVALCLRALEEVLN
CHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]