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The map label for this gene is selB [H]

Identifier: 113968452

GI number: 113968452

Start: 120062

End: 122083

Strand: Direct

Name: selB [H]

Synonym: Shewmr4_0107

Alternate gene names: 113968452

Gene position: 120062-122083 (Clockwise)

Preceding gene: 113968451

Following gene: 113968453

Centisome position: 2.55

GC content: 55.04

Gene sequence:

>2022_bases
ATGATTATGGTCACCGCGGGCCACGTCGACCACGGCAAGAGCACGCTGATCCGAGCCTTGACGGGGATGAACACCGACAG
GCTCCCCGAAGAAAAACGCCGTGGCATGACTATCGATCTCGGCTACGCCTTTATGCCTCTGCGAGATGGCACTCGCTTAG
CCTTTATCGATGTGCCCGGCCATGAAAAATTCATCAATAACATGCTGGTGGGCGTGAGCCATGTGCGCCACGCGCTGCTG
GTGTTGGCCTGTGACGACGGCGTGATGCCGCAAACCCGCGAGCATCTGCAAATCCTTGCGCTTTTACCGCTCAATAGTTT
GACCTTAGTGCTGACCAAGCGGGATTTGGTCGATGACCAAACCGCCGCCAAGTTGACCATAGAGGGAATCGCGCTGCTTG
CCGAATACGGGATTGAGGCCAGTGGCGTATTTGAGGTTTGCGCGAATGATACAGGTGATGCGGGGGTAGAAACCCTTAAA
CAACATATTTTAGAGATCGCCGAGCAGCAATCGACCCAAGCGGAAGATGCCAGCAGCTTTAGGATGACGCTCGATCGCGC
CTTTAGCGTTAAAGGTGCGGGCTGTGTGGTCACGGGCACTGTGATTAGCGGCAGTGTGTGTATCGGCGATAGCCTCTACA
GTTCAGGCCAAAAGGCCAAACTAAGGGTGCGCGGCATTCACTGCCAAGGGATGGAAGTCCATCGAGCCTTAAGTGGCACT
CGGGTCGCGTTGAATCTTACCGGTGTTGATAACCATAGGGCGCCCGCCCGCGGTGACTGGTTAAGCAGCCTGCCTCAGGC
TGAGCTGTGCGCCAGACCCGTAGTGCAAATTCGCAGTTTTGAACCCTTAACCCATTGGCAAAATGTGCATTGTCACCACG
GCGCCGACCATACCCTGGGTCGAGTGTCACTACTCGATGAAGCCGATGAGCAGGGGCGTTATCTGGCCGAAATAGTGCTC
GATAAGCCGCTGCTGCTATGTCAGGACGACCCGATAGTGCTCAGGCATATTGGCGGTAAGCAAACGCTGGGTGCAGGGCG
AGTGCTGGCATTAAAAGTCCCCAATCGTAAGAAGCGTACGCCGGAGCGTGTCAGTAAGCTACAACAGCTGGCCAAGCTCT
CCAATCCGGTGAATGTGCTGGCGCTACTCGGCCAGAGTGAGGCGCTCAGTATTGATGAAATCCGTTGGCGCTGGCAGCTA
ACCGATGAAGGGCTCGCAGCCATGCTGGCGCAGGCGGGGCTGACTCGCCTCGGTGCCTATGGGCTATCGACCCAGCTACT
CGAGCAAGAAAAACAGCAGTTTATCGAGCTGTTACGTGAGTATCACCAACAGCATCCCGACCAACTAGGGCTAGGGCGTA
ATCGTCTACAACGCATGAGCCACAGCATATTACCCAACGAGCTGGCCAATAAAGTGCTCGATAGCCTCTGCCAAGATGGA
CTGATGGTGCTGCGCGGCGCCTTGCTACAGTTGGCGGACCACAGGATTGTCCTCGATAGCGAGGCGCAGCAGTGCTGGCA
ACGCTTGGCTCCTTGGTTGGCGCTGCAAACCTCGCCCGTGTGGGTGACTGAGATGGGGGAATATCTCGAACTCGAGCCCG
CCAAACTCAGATTGCTGTGTTTTCAACTGGTGCAACAGGGTTTTATTACCGCTGTGGTGAAGGATAGGTATCTGCTGAGT
GAGCAGTTGTGCCACTACGCCAATCTGGTGCGTGAACATGTGGAAACCCATGGCAAGCTCGAAACCGCAGAGTTTAAGGA
TCTGATTGGCCTAGGCCGTAAGGTCAGTATTCAGCTGTTGGAGTTTTTTGACCGCAGTGGTTTTACCCGTAGGAAGTATC
GCAGTAACAGTCGGGAGATCCGGGATGGGGAATTGTTTTATTCCCATGAGAATTGGCGGAAGAACATAGGAGTCGAACCT
ACCCAGGACTGCTGGCAGCCCTACCCGGATTTGAAATCCGGACGCCTCACCGGAGACGACGTTCTTCCATTCGAAGACAG
ACCGATAGTAGAAAATGCTTAA

Upstream 100 bases:

>100_bases
TGATTGGCCGTATGACCCAAGATCAACTTCGTCTCGATTTGCGCGGTATCGATGATGAGGCCGAATTGTTAGCCGAACTC
AGCACCTTAGGTGTGCAGCT

Downstream 100 bases:

>100_bases
ACCTGAATCAAGCGCATTCGTATTTCGTTAGTCGCTTTGTGACTTGAGTCGAACTTTACTGGCAAACCAATTGTTTTTAT
TTGAATTAATGAGTTTAGAG

Product: selenocysteine-specific translation elongation factor SelB

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPGHEKFINNMLVGVSHVRHALL
VLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQTAAKLTIEGIALLAEYGIEASGVFEVCANDTGDAGVETLK
QHILEIAEQQSTQAEDASSFRMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLGRVSLLDEADEQGRYLAEIVL
DKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVSKLQQLAKLSNPVNVLALLGQSEALSIDEIRWRWQL
TDEGLAAMLAQAGLTRLGAYGLSTQLLEQEKQQFIELLREYHQQHPDQLGLGRNRLQRMSHSILPNELANKVLDSLCQDG
LMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLALQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAVVKDRYLLS
EQLCHYANLVREHVETHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEP
TQDCWQPYPDLKSGRLTGDDVLPFEDRPIVENA

Sequences:

>Translated_673_residues
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPGHEKFINNMLVGVSHVRHALL
VLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQTAAKLTIEGIALLAEYGIEASGVFEVCANDTGDAGVETLK
QHILEIAEQQSTQAEDASSFRMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLGRVSLLDEADEQGRYLAEIVL
DKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVSKLQQLAKLSNPVNVLALLGQSEALSIDEIRWRWQL
TDEGLAAMLAQAGLTRLGAYGLSTQLLEQEKQQFIELLREYHQQHPDQLGLGRNRLQRMSHSILPNELANKVLDSLCQDG
LMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLALQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAVVKDRYLLS
EQLCHYANLVREHVETHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEP
TQDCWQPYPDLKSGRLTGDDVLPFEDRPIVENA
>Mature_673_residues
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPGHEKFINNMLVGVSHVRHALL
VLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQTAAKLTIEGIALLAEYGIEASGVFEVCANDTGDAGVETLK
QHILEIAEQQSTQAEDASSFRMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLGRVSLLDEADEQGRYLAEIVL
DKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRTPERVSKLQQLAKLSNPVNVLALLGQSEALSIDEIRWRWQL
TDEGLAAMLAQAGLTRLGAYGLSTQLLEQEKQQFIELLREYHQQHPDQLGLGRNRLQRMSHSILPNELANKVLDSLCQDG
LMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLALQTSPVWVTEMGEYLELEPAKLRLLCFQLVQQGFITAVVKDRYLLS
EQLCHYANLVREHVETHGKLETAEFKDLIGLGRKVSIQLLEFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEP
TQDCWQPYPDLKSGRLTGDDVLPFEDRPIVENA

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI54607086, Length=358, Percent_Identity=26.8156424581006, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI34147630, Length=260, Percent_Identity=31.5384615384615, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI53729339, Length=284, Percent_Identity=27.4647887323944, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729337, Length=284, Percent_Identity=27.4647887323944, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI2367247, Length=626, Percent_Identity=42.0127795527157, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1790412, Length=275, Percent_Identity=32, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789737, Length=275, Percent_Identity=32, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789108, Length=146, Percent_Identity=30.8219178082192, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=377, Percent_Identity=26.525198938992, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556456, Length=278, Percent_Identity=31.6546762589928, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506081, Length=259, Percent_Identity=30.1158301158301, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=271, Percent_Identity=31.7343173431734, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19921738, Length=271, Percent_Identity=32.1033210332103, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281363316, Length=229, Percent_Identity=31.8777292576419, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17864358, Length=229, Percent_Identity=31.8777292576419, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922690, Length=330, Percent_Identity=28.1818181818182, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550900, Length=297, Percent_Identity=24.2424242424242, Blast_Score=76, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28572034, Length=97, Percent_Identity=37.1134020618557, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 75232; Mature: 75232

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00039 DEAD_ATP_HELICASE ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEEEECCCCEEEEEECCC
HEKFINNMLVGVSHVRHALLVLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQ
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHEEEEEECCCCEEEEEEECHHCCCC
TAAKLTIEGIALLAEYGIEASGVFEVCANDTGDAGVETLKQHILEIAEQQSTQAEDASSF
HHHHEEHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHCHHHH
RMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
HHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHCCCCCEEEEEEEEEECCHHHHHHCCCC
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLG
EEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCH
RVSLLDEADEQGRYLAEIVLDKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRT
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCC
PERVSKLQQLAKLSNPVNVLALLGQSEALSIDEIRWRWQLTDEGLAAMLAQAGLTRLGAY
HHHHHHHHHHHHHCCCCEEHHEECCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHHHHHC
GLSTQLLEQEKQQFIELLREYHQQHPDQLGLGRNRLQRMSHSILPNELANKVLDSLCQDG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
LMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLALQTSPVWVTEMGEYLELEPAKLRLLC
HHHHHHHHHHHHCCEEEECCHHHHHHHHHCHHHEECCCCEEHCCCCCHHCCCHHHHHHHH
FQLVQQGFITAVVKDRYLLSEQLCHYANLVREHVETHGKLETAEFKDLIGLGRKVSIQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHH
EFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEPTQDCWQPYPDLKSGRLTGDD
HHHHCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
VLPFEDRPIVENA
CCCCCCCCCCCCC
>Mature Secondary Structure
MIMVTAGHVDHGKSTLIRALTGMNTDRLPEEKRRGMTIDLGYAFMPLRDGTRLAFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEEEECCCCEEEEEECCC
HEKFINNMLVGVSHVRHALLVLACDDGVMPQTREHLQILALLPLNSLTLVLTKRDLVDDQ
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHEEEEEECCCCEEEEEEECHHCCCC
TAAKLTIEGIALLAEYGIEASGVFEVCANDTGDAGVETLKQHILEIAEQQSTQAEDASSF
HHHHEEHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHCHHHH
RMTLDRAFSVKGAGCVVTGTVISGSVCIGDSLYSSGQKAKLRVRGIHCQGMEVHRALSGT
HHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHCCCCCEEEEEEEEEECCHHHHHHCCCC
RVALNLTGVDNHRAPARGDWLSSLPQAELCARPVVQIRSFEPLTHWQNVHCHHGADHTLG
EEEEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCH
RVSLLDEADEQGRYLAEIVLDKPLLLCQDDPIVLRHIGGKQTLGAGRVLALKVPNRKKRT
HHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCC
PERVSKLQQLAKLSNPVNVLALLGQSEALSIDEIRWRWQLTDEGLAAMLAQAGLTRLGAY
HHHHHHHHHHHHHCCCCEEHHEECCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHHHHHC
GLSTQLLEQEKQQFIELLREYHQQHPDQLGLGRNRLQRMSHSILPNELANKVLDSLCQDG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
LMVLRGALLQLADHRIVLDSEAQQCWQRLAPWLALQTSPVWVTEMGEYLELEPAKLRLLC
HHHHHHHHHHHHCCEEEECCHHHHHHHHHCHHHEECCCCEEHCCCCCHHCCCHHHHHHHH
FQLVQQGFITAVVKDRYLLSEQLCHYANLVREHVETHGKLETAEFKDLIGLGRKVSIQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHH
EFFDRSGFTRRKYRSNSREIRDGELFYSHENWRKNIGVEPTQDCWQPYPDLKSGRLTGDD
HHHHCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
VLPFEDRPIVENA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]