Click here to switch to the map view.

The map label for this gene is pbpG [H]

Identifier: 116695901

GI number: 116695901

Start: 2217731

End: 2219842

Strand: Direct

Name: pbpG [H]

Synonym: H16_B1965

Alternate gene names: 116695901

Gene position: 2217731-2219842 (Clockwise)

Preceding gene: 116695900

Following gene: 116695902

Centisome position: 76.15

GC content: 70.69

Gene sequence:

>2112_bases
ATGAAAAAAATCGCAGTCAAGGCAGGCGGCGCCGTACTGGCGCTGGCTGCAGCCGGTGCCTTGCTTGCCGGCTTTGCCGC
GGTGGTAAGCCTGCGGCAGCTTCCCGCAGTCGACTCCCTGCGCGACTACCGGCCCGACGTGCCCCTGCGGATCTACACCA
GCGACAATGTGCAGATCGGCGAGTTCGGCAGCGAGCACCGCGAGTTCATCCCCTTCGGGCAGATGCCGTCGCGCATGGTC
CAGGCGCTGCTGGCTGCCGAGGACGACCAGTTCTACGAGCACGAGGGTGTCGACATGCCCGGCGTGCTGCGCGCCGCGCT
GGCCAACCTGGGCCAGGGCTATGCGCAGGGCGCGTCCACCATCACCATGCAGGTGGCGCGCAATTTCTACCTGTCGCGCG
AGAAGACGCTGGCGCGCAAGTGGTATGAAATGCTGCTGGCCTGGCGCATCGACCAGACCCTGCCCAAGGACCGCATCCTT
GAGCTGTACATGAACCAGGTCTACCTGGGCGAGCATGCCTACGGCTTCGGCAGCGCCGCGCATGCGTACTTCGGCAAGCC
GCTGGCGTCGTTGTCGCTGGCCGAGGCGGCGATGCTGGCGGGGCTGCCCAAGGCCCCTTCGTCCATCAACCCGGTGGTCA
ACTTCCCGCGCGCCAAGCGGCGCCAGGAATATGTGCTGGGCCGCATGCTGGCACTGGGCATGATCAGCCAGGACGACTAC
CGCGAGGCCCGCGCCGCGCGCCTGGCAATCTCCGACGACAGCCCCGGCAGCTTTGCCGGGCATGCCGAGTACGTGGCCGA
ACTCGCGCGCCAGCTGGCGCGCGAGCGCTTTGGCGACGAGGCCTACACGCGCGGCCTGAACGTCTACACCACGGTGTCGT
CGGTGCGCCAGACCCTGGCTTACGATGCGGTGCACGGCGGGCTGGAAGGCTACGCGCGGCGCCACCGCAAGTTCGTCAAG
GACCTGTCGCAGGGCCCGCAGGGTGCGCTGGTATCGCTGGACAGCGCCACCGGCGCGATCGAGGCGATGGTGGGCGGCGC
CGACTTTGCCACCAGCCGCTTCAACCACGCCACCCAGGCGCAGCGCCAGCCGGGCTCGACCTTCAAGCCCTTCGTCTATG
CGGCGGCACTGGAGCGCGGCGTGTCGCCCGGCACGCTGATCAACGACGCGCCGCTGCCCAATGGTTCGCGCTGGCAGCCG
TCCAATGACGACGGGCGCTTTGTCGGCTCCATCACCGTGCGCCAGGCGCTGGCGGAATCGCGCAACCTGCCGGCGATCCG
CACGCTGCAGGCGATCGGCATCCCGTACACGGTGGAGTTCGCCAGCCGTTTCGGCTTCGCGCCCAGGCGGCTGCCGCGCT
ACCTGCCGCTGGCACTGGGCACCGGCACCACTTCGCCGCTGCGCCTGGCCGCGGCCTATGGCGTCTTTGCCAACGGCGGC
CACCGCATCGAGCCCTACCTGATCGCGCGCATCACCGACAGCGAAGGCAAGGTGCTGTTCTCTGCCGAGTCGGAGGACGC
CGCGGACAGCCCGGCCCCGCCGCGCGTGATCAGCGCCCGCAACGCCTTCGTCATGGACAGCCTGCTGCGCAGCGTGGTCG
ACGACGGCACCGGCGCCGGCGTGCGCCGCTACCTGCGCCGCGCCGACGTGGCCGGCAAGACCGGCACCACCAATGACTCG
GTCGACGGCTGGTTCGCGGGCTACGCCGGCGGCGTCGTCACGGTGGCGTGGATGGGCTACGACGACAACCGCAGCCTGGG
CGAGCATGAGTTCGGCGCCACCACCGCGCTGCCGGTGTGGGCCGCCTATATGGAGGGCCGCCTTGCGGGCGTGCCCGTGC
CTGAAGCTGAAGCGCCCGCCGGCGTGGTCCGCGTGGGAGATGACTGGATGTACGCGGAGTATGCCGATGGCAGCCACGGC
ATCGCCGCCATCGGCTTCCCGCCGCCGGCACCCGCGGCGGCGCCGGCGGTCGATCCGGTCGCCAGCAACCTCGGCGGGAC
CGCGGCGCCGGCCCCGGCTGCCCCCGCGCCGACACCTGTCACCAGCGAGACAACTGGCCTGCCGGCGCCCGGCGCTGCCA
CGCCGGCCGTCCCCGCGGACGCCAGGCTGTAG

Upstream 100 bases:

>100_bases
CGCCACCGGCACGCCGCCCATCGACCATGACGAGCTCGGCAACCCGCTGCCGCTGGCGGCCGGCCGCTAGCGCCAGTCAG
GCAGCGGGTAAAATGCGCGC

Downstream 100 bases:

>100_bases
ACTTCCGGCTTTCCACTGTCCCCGATCCCGATGTCCAGCCACGCCCCGCTGCTGATCTGGTCGGTCGCCGCGCTGACCAC
GGCCGGCGTCCTGTTCCGCC

Product: periplasmic multimodular transpeptidase/transglycosylase

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MKKIAVKAGGAVLALAAAGALLAGFAAVVSLRQLPAVDSLRDYRPDVPLRIYTSDNVQIGEFGSEHREFIPFGQMPSRMV
QALLAAEDDQFYEHEGVDMPGVLRAALANLGQGYAQGASTITMQVARNFYLSREKTLARKWYEMLLAWRIDQTLPKDRIL
ELYMNQVYLGEHAYGFGSAAHAYFGKPLASLSLAEAAMLAGLPKAPSSINPVVNFPRAKRRQEYVLGRMLALGMISQDDY
REARAARLAISDDSPGSFAGHAEYVAELARQLARERFGDEAYTRGLNVYTTVSSVRQTLAYDAVHGGLEGYARRHRKFVK
DLSQGPQGALVSLDSATGAIEAMVGGADFATSRFNHATQAQRQPGSTFKPFVYAAALERGVSPGTLINDAPLPNGSRWQP
SNDDGRFVGSITVRQALAESRNLPAIRTLQAIGIPYTVEFASRFGFAPRRLPRYLPLALGTGTTSPLRLAAAYGVFANGG
HRIEPYLIARITDSEGKVLFSAESEDAADSPAPPRVISARNAFVMDSLLRSVVDDGTGAGVRRYLRRADVAGKTGTTNDS
VDGWFAGYAGGVVTVAWMGYDDNRSLGEHEFGATTALPVWAAYMEGRLAGVPVPEAEAPAGVVRVGDDWMYAEYADGSHG
IAAIGFPPPAPAAAPAVDPVASNLGGTAAPAPAAPAPTPVTSETTGLPAPGAATPAVPADARL

Sequences:

>Translated_703_residues
MKKIAVKAGGAVLALAAAGALLAGFAAVVSLRQLPAVDSLRDYRPDVPLRIYTSDNVQIGEFGSEHREFIPFGQMPSRMV
QALLAAEDDQFYEHEGVDMPGVLRAALANLGQGYAQGASTITMQVARNFYLSREKTLARKWYEMLLAWRIDQTLPKDRIL
ELYMNQVYLGEHAYGFGSAAHAYFGKPLASLSLAEAAMLAGLPKAPSSINPVVNFPRAKRRQEYVLGRMLALGMISQDDY
REARAARLAISDDSPGSFAGHAEYVAELARQLARERFGDEAYTRGLNVYTTVSSVRQTLAYDAVHGGLEGYARRHRKFVK
DLSQGPQGALVSLDSATGAIEAMVGGADFATSRFNHATQAQRQPGSTFKPFVYAAALERGVSPGTLINDAPLPNGSRWQP
SNDDGRFVGSITVRQALAESRNLPAIRTLQAIGIPYTVEFASRFGFAPRRLPRYLPLALGTGTTSPLRLAAAYGVFANGG
HRIEPYLIARITDSEGKVLFSAESEDAADSPAPPRVISARNAFVMDSLLRSVVDDGTGAGVRRYLRRADVAGKTGTTNDS
VDGWFAGYAGGVVTVAWMGYDDNRSLGEHEFGATTALPVWAAYMEGRLAGVPVPEAEAPAGVVRVGDDWMYAEYADGSHG
IAAIGFPPPAPAAAPAVDPVASNLGGTAAPAPAAPAPTPVTSETTGLPAPGAATPAVPADARL
>Mature_703_residues
MKKIAVKAGGAVLALAAAGALLAGFAAVVSLRQLPAVDSLRDYRPDVPLRIYTSDNVQIGEFGSEHREFIPFGQMPSRMV
QALLAAEDDQFYEHEGVDMPGVLRAALANLGQGYAQGASTITMQVARNFYLSREKTLARKWYEMLLAWRIDQTLPKDRIL
ELYMNQVYLGEHAYGFGSAAHAYFGKPLASLSLAEAAMLAGLPKAPSSINPVVNFPRAKRRQEYVLGRMLALGMISQDDY
REARAARLAISDDSPGSFAGHAEYVAELARQLARERFGDEAYTRGLNVYTTVSSVRQTLAYDAVHGGLEGYARRHRKFVK
DLSQGPQGALVSLDSATGAIEAMVGGADFATSRFNHATQAQRQPGSTFKPFVYAAALERGVSPGTLINDAPLPNGSRWQP
SNDDGRFVGSITVRQALAESRNLPAIRTLQAIGIPYTVEFASRFGFAPRRLPRYLPLALGTGTTSPLRLAAAYGVFANGG
HRIEPYLIARITDSEGKVLFSAESEDAADSPAPPRVISARNAFVMDSLLRSVVDDGTGAGVRRYLRRADVAGKTGTTNDS
VDGWFAGYAGGVVTVAWMGYDDNRSLGEHEFGATTALPVWAAYMEGRLAGVPVPEAEAPAGVVRVGDDWMYAEYADGSHG
IAAIGFPPPAPAAAPAVDPVASNLGGTAAPAPAAPAPTPVTSETTGLPAPGAATPAVPADARL

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=576, Percent_Identity=34.8958333333333, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=43.9739413680782, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1788867, Length=546, Percent_Identity=30.5860805860806, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1789601, Length=171, Percent_Identity=33.9181286549708, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 74729; Mature: 74729

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAVKAGGAVLALAAAGALLAGFAAVVSLRQLPAVDSLRDYRPDVPLRIYTSDNVQIG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCEEEC
EFGSEHREFIPFGQMPSRMVQALLAAEDDQFYEHEGVDMPGVLRAALANLGQGYAQGAST
CCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCHHHCCCHH
ITMQVARNFYLSREKTLARKWYEMLLAWRIDQTLPKDRILELYMNQVYLGEHAYGFGSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCCCHH
HAYFGKPLASLSLAEAAMLAGLPKAPSSINPVVNFPRAKRRQEYVLGRMLALGMISQDDY
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
REARAARLAISDDSPGSFAGHAEYVAELARQLARERFGDEAYTRGLNVYTTVSSVRQTLA
HHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHEEHHHHHHHHHH
YDAVHGGLEGYARRHRKFVKDLSQGPQGALVSLDSATGAIEAMVGGADFATSRFNHATQA
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHH
QRQPGSTFKPFVYAAALERGVSPGTLINDAPLPNGSRWQPSNDDGRFVGSITVRQALAES
HCCCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
RNLPAIRTLQAIGIPYTVEFASRFGFAPRRLPRYLPLALGTGTTSPLRLAAAYGVFANGG
CCCCHHHHHHHHCCCEEEHHHHHCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCC
HRIEPYLIARITDSEGKVLFSAESEDAADSPAPPRVISARNAFVMDSLLRSVVDDGTGAG
CEECCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCHH
VRRYLRRADVAGKTGTTNDSVDGWFAGYAGGVVTVAWMGYDDNRSLGEHEFGATTALPVW
HHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
AAYMEGRLAGVPVPEAEAPAGVVRVGDDWMYAEYADGSHGIAAIGFPPPAPAAAPAVDPV
HHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCHH
ASNLGGTAAPAPAAPAPTPVTSETTGLPAPGAATPAVPADARL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIAVKAGGAVLALAAAGALLAGFAAVVSLRQLPAVDSLRDYRPDVPLRIYTSDNVQIG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCEEEC
EFGSEHREFIPFGQMPSRMVQALLAAEDDQFYEHEGVDMPGVLRAALANLGQGYAQGAST
CCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCHHHCCCHH
ITMQVARNFYLSREKTLARKWYEMLLAWRIDQTLPKDRILELYMNQVYLGEHAYGFGSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCCCHH
HAYFGKPLASLSLAEAAMLAGLPKAPSSINPVVNFPRAKRRQEYVLGRMLALGMISQDDY
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
REARAARLAISDDSPGSFAGHAEYVAELARQLARERFGDEAYTRGLNVYTTVSSVRQTLA
HHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHEEHHHHHHHHHH
YDAVHGGLEGYARRHRKFVKDLSQGPQGALVSLDSATGAIEAMVGGADFATSRFNHATQA
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHH
QRQPGSTFKPFVYAAALERGVSPGTLINDAPLPNGSRWQPSNDDGRFVGSITVRQALAES
HCCCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
RNLPAIRTLQAIGIPYTVEFASRFGFAPRRLPRYLPLALGTGTTSPLRLAAAYGVFANGG
CCCCHHHHHHHHCCCEEEHHHHHCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCC
HRIEPYLIARITDSEGKVLFSAESEDAADSPAPPRVISARNAFVMDSLLRSVVDDGTGAG
CEECCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCHH
VRRYLRRADVAGKTGTTNDSVDGWFAGYAGGVVTVAWMGYDDNRSLGEHEFGATTALPVW
HHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
AAYMEGRLAGVPVPEAEAPAGVVRVGDDWMYAEYADGSHGIAAIGFPPPAPAAAPAVDPV
HHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCHH
ASNLGGTAAPAPAAPAPTPVTSETTGLPAPGAATPAVPADARL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]