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The map label for this gene is moaA2 [H]

Identifier: 116695408

GI number: 116695408

Start: 1644558

End: 1645703

Strand: Direct

Name: moaA2 [H]

Synonym: H16_B1466

Alternate gene names: 116695408

Gene position: 1644558-1645703 (Clockwise)

Preceding gene: 116695407

Following gene: 116695415

Centisome position: 56.47

GC content: 68.67

Gene sequence:

>1146_bases
ATGGCATGCGCCATGCCCTACCCCATTGCCCCCGGGAGATTCGCCATGCAAGCAGCGCTTGTGCAAGAAATGCCCGCCGC
CCGCAGTCTTGAGGCGCCCGAGCCGGACCACGCATCACCCGCCTGCCGGGACCAGCTGGGCCGTCCGCTGCGCGACCTGC
GGCTCTCGGTGATCGACCAGTGCAACTTCCGCTGCACCTACTGCATGCCCAAGGAGCGCTTCGGGCGCGACTACCCGTTC
CTGTCGCCTGAGCAGCGCCTGTCCGATGCGGAACTGCTGCGCATCGTGCGCGCCTTTGTCGGCCTGGGCGTTGAGAAAGT
GCGCCTGACCGGCGGCGAGCCGCTGCTGCGCAAGGGCATCGAGTCGCTGGTCGAACGCATTGCGGCCATGCGTACCCTGG
ACGGCCGGCAGGTGGAAGTGGCCATGACCACCAACGGCAGCCTGCTGGCCCGCAAGGCGCGTTCGCTGCGCGACGCAGGC
CTGGGCCGGGTCACGGTCAGCCTGGACAGCCTGGACGACCAGATCTTCCGCGCCATGAACGATGTCGATTTCCCGGTCGG
GCGCGTGCTGGAGGGCATCGACGCGGCCATCGCGGCGGGCCTGGCACCAGTCAAGGTCAACTGCGTGGTCGAGCGCGGCA
CCAACGACGCGCAGGTGCTGCCGCTGGTGGAGCACTTCCGTGGCAGCGACGTCACGCTGCGCTTTATCGAATATATGGAT
GTGGAAGGCCCGAGCGGCTGGTCACAGTCACGCGTCGTCCCGTCCGACGAGATCCGCGGCATGGTCGAACGCGCGCATGC
GCTGCTGCCGGTCACCCGGCGCGACGGCGAAACCGCCAGCAACTACCTGCTGGCGGATGGCAGCCTGAAGCTGGGTTTTA
TCTCCAGCGTTTCGCACCCGTTCTGCGGCGATTGCACCCGCGCACGCGTTTCGGTCGACGGTCGGCTTTACCTGTGCCTG
TTCGCCACCAGCTCTGTCGACCTGCGCCAGCACCTGGGTGCCGCGCGCCCCGATGAAGCCGTTGCGGACGCGGTCCGGCA
AGCCTGGCAGGCCCGCGGCGACCGCTACTCCGAGCTGCGCGCGGACCGGCTGGCCAGCGGCAAGCGCCAGTACCCGACGG
TGCGCATGTCGCTGGTCGGCGGCTGA

Upstream 100 bases:

>100_bases
GCCCCGACGCAGCCATACCGGCCCCGCCGGAACTGTTCTGCTTCCGCTCCTGCCGATTTGTACGCCTGCCGGGCGGCGAT
CGGTCCCTACCATGGAGGGC

Downstream 100 bases:

>100_bases
GCCGCGGGCGCGGCTCTCGGCCACTCAGTCAGGATTCGGGAAAGCCTGCGCGGCAAGCTCCGCGCCGGCTGGTGTCAGGC
TCGCGTGACCGCGGCCTTCC

Product: molybdopterin biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MACAMPYPIAPGRFAMQAALVQEMPAARSLEAPEPDHASPACRDQLGRPLRDLRLSVIDQCNFRCTYCMPKERFGRDYPF
LSPEQRLSDAELLRIVRAFVGLGVEKVRLTGGEPLLRKGIESLVERIAAMRTLDGRQVEVAMTTNGSLLARKARSLRDAG
LGRVTVSLDSLDDQIFRAMNDVDFPVGRVLEGIDAAIAAGLAPVKVNCVVERGTNDAQVLPLVEHFRGSDVTLRFIEYMD
VEGPSGWSQSRVVPSDEIRGMVERAHALLPVTRRDGETASNYLLADGSLKLGFISSVSHPFCGDCTRARVSVDGRLYLCL
FATSSVDLRQHLGAARPDEAVADAVRQAWQARGDRYSELRADRLASGKRQYPTVRMSLVGG

Sequences:

>Translated_381_residues
MACAMPYPIAPGRFAMQAALVQEMPAARSLEAPEPDHASPACRDQLGRPLRDLRLSVIDQCNFRCTYCMPKERFGRDYPF
LSPEQRLSDAELLRIVRAFVGLGVEKVRLTGGEPLLRKGIESLVERIAAMRTLDGRQVEVAMTTNGSLLARKARSLRDAG
LGRVTVSLDSLDDQIFRAMNDVDFPVGRVLEGIDAAIAAGLAPVKVNCVVERGTNDAQVLPLVEHFRGSDVTLRFIEYMD
VEGPSGWSQSRVVPSDEIRGMVERAHALLPVTRRDGETASNYLLADGSLKLGFISSVSHPFCGDCTRARVSVDGRLYLCL
FATSSVDLRQHLGAARPDEAVADAVRQAWQARGDRYSELRADRLASGKRQYPTVRMSLVGG
>Mature_380_residues
ACAMPYPIAPGRFAMQAALVQEMPAARSLEAPEPDHASPACRDQLGRPLRDLRLSVIDQCNFRCTYCMPKERFGRDYPFL
SPEQRLSDAELLRIVRAFVGLGVEKVRLTGGEPLLRKGIESLVERIAAMRTLDGRQVEVAMTTNGSLLARKARSLRDAGL
GRVTVSLDSLDDQIFRAMNDVDFPVGRVLEGIDAAIAAGLAPVKVNCVVERGTNDAQVLPLVEHFRGSDVTLRFIEYMDV
EGPSGWSQSRVVPSDEIRGMVERAHALLPVTRRDGETASNYLLADGSLKLGFISSVSHPFCGDCTRARVSVDGRLYLCLF
ATSSVDLRQHLGAARPDEAVADAVRQAWQARGDRYSELRADRLASGKRQYPTVRMSLVGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=315, Percent_Identity=37.1428571428571, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI22027536, Length=315, Percent_Identity=37.1428571428571, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI1786999, Length=309, Percent_Identity=37.5404530744337, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32566329, Length=364, Percent_Identity=32.967032967033, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI32566331, Length=346, Percent_Identity=33.5260115606936, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17561210, Length=340, Percent_Identity=32.6470588235294, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28574490, Length=317, Percent_Identity=33.4384858044164, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI28574488, Length=323, Percent_Identity=34.0557275541796, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41800; Mature: 41669

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACAMPYPIAPGRFAMQAALVQEMPAARSLEAPEPDHASPACRDQLGRPLRDLRLSVIDQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHC
CNFRCTYCMPKERFGRDYPFLSPEQRLSDAELLRIVRAFVGLGVEKVRLTGGEPLLRKGI
CCCEEEEECCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
ESLVERIAAMRTLDGRQVEVAMTTNGSLLARKARSLRDAGLGRVTVSLDSLDDQIFRAMN
HHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
DVDFPVGRVLEGIDAAIAAGLAPVKVNCVVERGTNDAQVLPLVEHFRGSDVTLRFIEYMD
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHCCCCCEEEEEEEHHC
VEGPSGWSQSRVVPSDEIRGMVERAHALLPVTRRDGETASNYLLADGSLKLGFISSVSHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCEEEEEHHCCCCC
FCGDCTRARVSVDGRLYLCLFATSSVDLRQHLGAARPDEAVADAVRQAWQARGDRYSELR
CCCCCCCEEEEECCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ADRLASGKRQYPTVRMSLVGG
HHHHHCCCCCCCEEEEEECCC
>Mature Secondary Structure 
ACAMPYPIAPGRFAMQAALVQEMPAARSLEAPEPDHASPACRDQLGRPLRDLRLSVIDQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHC
CNFRCTYCMPKERFGRDYPFLSPEQRLSDAELLRIVRAFVGLGVEKVRLTGGEPLLRKGI
CCCEEEEECCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
ESLVERIAAMRTLDGRQVEVAMTTNGSLLARKARSLRDAGLGRVTVSLDSLDDQIFRAMN
HHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
DVDFPVGRVLEGIDAAIAAGLAPVKVNCVVERGTNDAQVLPLVEHFRGSDVTLRFIEYMD
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHCCCCCEEEEEEEHHC
VEGPSGWSQSRVVPSDEIRGMVERAHALLPVTRRDGETASNYLLADGSLKLGFISSVSHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCEEEEEHHCCCCC
FCGDCTRARVSVDGRLYLCLFATSSVDLRQHLGAARPDEAVADAVRQAWQARGDRYSELR
CCCCCCCEEEEECCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ADRLASGKRQYPTVRMSLVGG
HHHHHCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]