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The map label for this gene is hipO [H]

Identifier: 116695415

GI number: 116695415

Start: 1653228

End: 1654409

Strand: Direct

Name: hipO [H]

Synonym: H16_B1473

Alternate gene names: 116695415

Gene position: 1653228-1654409 (Clockwise)

Preceding gene: 116695408

Following gene: 116695416

Centisome position: 56.76

GC content: 65.4

Gene sequence:

>1182_bases
ATGAACCAGGCCCCGCTTGCCGAACTGCACCAATCCATCCAGACCCTGTCGCCGGAGTTCGTTGAGATCCGCCGAAAGAT
CCATGCGCACCCCGAACTGGCCTTTGAAGAGCGCCAGACCAGCGACCTGGTCGCCGGACGGCTGGCGGCATGGGGCTACG
AGGTGCATCGCGGCATGGGCACCACCGGCGTCGTGGGCAGGCTAAGGAAGGGGCAAGGCAGCAAGGCGCTGGGCATCCGC
GCCGACATGGACGCGCTGCCGATTCAGGAAAAGACCGGCCTTGACTACGCCAGCACCATCGCCGGCAAGATGCACGCCTG
CGGCCATGACGGGCACACGGCGATCCTGCTGTGTGCCGCCAGGTACCTGTCCGAGTCCGTTAATTTCAATGGCACGCTCA
ACCTGATCTTCCAGCCGGCCGAGGAGAACGAAGGCGGTGCGCTGCGCATGCTGGACGATGGGCTGTTCGAGCAATTCCCC
TGCGACGAAATCTATGCGCTGCACAATTCCCCGGGGCTGCCGGTCGGCCAGATCGGGGTCATCGCCGGCCCTGCCATGGC
CTCGTTCGACCGCGCCACGGTCACGCTGCGCGGCCGCGGCGCCCATGGCGCCATGCCTCATCATGGCATCGACGCCATGC
AGTGCGCCGCCAGCATCGTGCTTGGACTGCAATCGATCGTCAGCCGCGAGATCGATGCGCTCAAGTCCGCGGTCATTACC
GTAGGCTCCATCCAGGCTGGCACCACCTACAACGTCGTGCCCGACAGCGCAACGATCAAGATCGGCGTGCGCACGCTGGA
CCCGCAGGTGCGTACGCTGGTCGAAGCGCGCATCAGCGAATTTGTCGCCGCACAGGCCCAGAGCTTCCGGCTGCAATCCG
AGGTCATCTACGAACGCAAGTACCCGGTACTGGTCAACCACGACGCGCAGACCGAGCGCGCGCGCCAGGCCGCGATCCGG
CTGGTGGGCGCGGACAACGTGGTCGAGCGCCCGCCGGTGATGGGCAGCGAAGACTTCGCCTACATGCTGGAGCACCGGCC
CGGTGCCTACATCCGGCTCGGCAACGGCCTGGGCGAGGATGGTGGTTGCATGGTCCACAACCCCCTGTACGACTTCAATG
ACAAGGCCCTGCCTGTCGGCGCCGCATTCTGGGCGCACCTGACGCAAAGCTATCTGGTTTGA

Upstream 100 bases:

>100_bases
TAATGTTGCAAATTATGCAACGTTTCTGCCTCATCATTGTCATGGTTTCGGCAAGCGCCTGCGTGGAGAATGCTGTGGAC
GACCACGCTCCGATACCGCC

Downstream 100 bases:

>100_bases
ACAACAATTCTTCGGGGACATCCACCATGGCTTCACTGAACCGCCGCCGCTTCCTTCAATATGCCGGTGTTTCCGCCGGC
ACCTCCGTGCTGGCCAGCCT

Product: metal-dependent amidase/aminoacylase/carboxypeptidase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNQAPLAELHQSIQTLSPEFVEIRRKIHAHPELAFEERQTSDLVAGRLAAWGYEVHRGMGTTGVVGRLRKGQGSKALGIR
ADMDALPIQEKTGLDYASTIAGKMHACGHDGHTAILLCAARYLSESVNFNGTLNLIFQPAEENEGGALRMLDDGLFEQFP
CDEIYALHNSPGLPVGQIGVIAGPAMASFDRATVTLRGRGAHGAMPHHGIDAMQCAASIVLGLQSIVSREIDALKSAVIT
VGSIQAGTTYNVVPDSATIKIGVRTLDPQVRTLVEARISEFVAAQAQSFRLQSEVIYERKYPVLVNHDAQTERARQAAIR
LVGADNVVERPPVMGSEDFAYMLEHRPGAYIRLGNGLGEDGGCMVHNPLYDFNDKALPVGAAFWAHLTQSYLV

Sequences:

>Translated_393_residues
MNQAPLAELHQSIQTLSPEFVEIRRKIHAHPELAFEERQTSDLVAGRLAAWGYEVHRGMGTTGVVGRLRKGQGSKALGIR
ADMDALPIQEKTGLDYASTIAGKMHACGHDGHTAILLCAARYLSESVNFNGTLNLIFQPAEENEGGALRMLDDGLFEQFP
CDEIYALHNSPGLPVGQIGVIAGPAMASFDRATVTLRGRGAHGAMPHHGIDAMQCAASIVLGLQSIVSREIDALKSAVIT
VGSIQAGTTYNVVPDSATIKIGVRTLDPQVRTLVEARISEFVAAQAQSFRLQSEVIYERKYPVLVNHDAQTERARQAAIR
LVGADNVVERPPVMGSEDFAYMLEHRPGAYIRLGNGLGEDGGCMVHNPLYDFNDKALPVGAAFWAHLTQSYLV
>Mature_393_residues
MNQAPLAELHQSIQTLSPEFVEIRRKIHAHPELAFEERQTSDLVAGRLAAWGYEVHRGMGTTGVVGRLRKGQGSKALGIR
ADMDALPIQEKTGLDYASTIAGKMHACGHDGHTAILLCAARYLSESVNFNGTLNLIFQPAEENEGGALRMLDDGLFEQFP
CDEIYALHNSPGLPVGQIGVIAGPAMASFDRATVTLRGRGAHGAMPHHGIDAMQCAASIVLGLQSIVSREIDALKSAVIT
VGSIQAGTTYNVVPDSATIKIGVRTLDPQVRTLVEARISEFVAAQAQSFRLQSEVIYERKYPVLVNHDAQTERARQAAIR
LVGADNVVERPPVMGSEDFAYMLEHRPGAYIRLGNGLGEDGGCMVHNPLYDFNDKALPVGAAFWAHLTQSYLV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=436, Percent_Identity=28.6697247706422, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42485; Mature: 42485

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAPLAELHQSIQTLSPEFVEIRRKIHAHPELAFEERQTSDLVAGRLAAWGYEVHRGMG
CCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTGVVGRLRKGQGSKALGIRADMDALPIQEKTGLDYASTIAGKMHACGHDGHTAILLCAA
CHHHHHHHHCCCCCCEEEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RYLSESVNFNGTLNLIFQPAEENEGGALRMLDDGLFEQFPCDEIYALHNSPGLPVGQIGV
HHHHHCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHCCCHHEEEEECCCCCCCCCCCE
IAGPAMASFDRATVTLRGRGAHGAMPHHGIDAMQCAASIVLGLQSIVSREIDALKSAVIT
EECCCHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VGSIQAGTTYNVVPDSATIKIGVRTLDPQVRTLVEARISEFVAAQAQSFRLQSEVIYERK
EECCCCCCEEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YPVLVNHDAQTERARQAAIRLVGADNVVERPPVMGSEDFAYMLEHRPGAYIRLGNGLGED
CCEEECCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCC
GGCMVHNPLYDFNDKALPVGAAFWAHLTQSYLV
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQAPLAELHQSIQTLSPEFVEIRRKIHAHPELAFEERQTSDLVAGRLAAWGYEVHRGMG
CCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTGVVGRLRKGQGSKALGIRADMDALPIQEKTGLDYASTIAGKMHACGHDGHTAILLCAA
CHHHHHHHHCCCCCCEEEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RYLSESVNFNGTLNLIFQPAEENEGGALRMLDDGLFEQFPCDEIYALHNSPGLPVGQIGV
HHHHHCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHCCCHHEEEEECCCCCCCCCCCE
IAGPAMASFDRATVTLRGRGAHGAMPHHGIDAMQCAASIVLGLQSIVSREIDALKSAVIT
EECCCHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VGSIQAGTTYNVVPDSATIKIGVRTLDPQVRTLVEARISEFVAAQAQSFRLQSEVIYERK
EECCCCCCEEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YPVLVNHDAQTERARQAAIRLVGADNVVERPPVMGSEDFAYMLEHRPGAYIRLGNGLGED
CCEEECCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCC
GGCMVHNPLYDFNDKALPVGAAFWAHLTQSYLV
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]