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The map label for this gene is iclR [H]

Identifier: 116694825

GI number: 116694825

Start: 996830

End: 997720

Strand: Direct

Name: iclR [H]

Synonym: H16_B0879

Alternate gene names: 116694825

Gene position: 996830-997720 (Clockwise)

Preceding gene: 116694823

Following gene: 116694826

Centisome position: 34.23

GC content: 69.81

Gene sequence:

>891_bases
GTGGCGCCTGCCGCCAGCGCCGACCCTCCGTCACGGGAAGGGCGGGAATGCCGGGACGGCGACAGCACGGTGCAGTCCCT
GACGCGCGCGCTCCAGTTGATCGAGCTGTTGTCCGATGACGACGAGGGCTACCGGCTGGTGGATCTGGCGGCACGCAGCG
GCCTGTCTACGTCGACCGTCCATCGCCTGCTGACCACTCTGGAGCAGCGCCAGTTTGTCCAGTTCGATCGCGAGCGCAGC
CTGTGGTACGTGGGCGTGCGTTGCTTCTCGGTGGGTGCCGCTTTCGCCCGCCGGCGCCGCATCAGCGAACTTGCCCTGCC
GGTGATGCAACGGTTGAGCGCGCTGTCCGGGGAGACCGTCAACCTGGGCGTGGCCAGCGACGGGCAGATTGTTTTCGCCT
GCCAGGCAGAGGGCCCCCACGGCAGCCGCGCGCTGGCCAGGCCGGGCCAGCGTGCGCCGCTGCATGGCACGGCCATGGGC
CAGGCGGTGCTGGCAGCCAAGAGCGAACCCGACGTGCTGCAATACCTGCGCACCTTTGGCTTGCCGCGCCTGACCAACAA
CACCATTGCGCGCCCCTCGCGCCTGCATGAGACCCTGGCCGAAGTGCGCCGGCGCGGCTATGCGGTGGACGACGAGCAGA
ATGCGGTCGGCCTGTGCTGCATTGCCGCCGCGATTTGTGACGAGCACGGCCGGCCCTTCGCCGCGGTCTCGCTGGCCGGT
TCCAGCCAGCGCATCGTGCGTGCCCGTTTCGATGAGCTTGGCGCCTGGGTGCGTTGGGCTGCCACAGAGATCAGCGCAGC
CTACGGCGGGCGCTGCACCCGCGTGCCCTGCCCGATGCCAGTGCCCGCAGCGTCCTTGCCTTTCCACGCGGGCGCGCCGG
AAAGCTGTTGA

Upstream 100 bases:

>100_bases
GGAACTTGCGTCCGCGTGCGGCAACGCAAGCAAGGGGACTCACATGGCAGGCACCACGACCAGGACAAGGGAGGCCAGGA
AGGAAGCCGCAACACGGGCG

Downstream 100 bases:

>100_bases
TTCTTAAGGGTTTATTCAAGAAGTTCCGGCGTGCGGGAAAGCCGCCGCGCGGGTCTGGCCATGCGCTTCAATGGAAGCAA
TGTCACGACCGGCCCCCGCA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAPAASADPPSREGRECRDGDSTVQSLTRALQLIELLSDDDEGYRLVDLAARSGLSTSTVHRLLTTLEQRQFVQFDRERS
LWYVGVRCFSVGAAFARRRRISELALPVMQRLSALSGETVNLGVASDGQIVFACQAEGPHGSRALARPGQRAPLHGTAMG
QAVLAAKSEPDVLQYLRTFGLPRLTNNTIARPSRLHETLAEVRRRGYAVDDEQNAVGLCCIAAAICDEHGRPFAAVSLAG
SSQRIVRARFDELGAWVRWAATEISAAYGGRCTRVPCPMPVPAASLPFHAGAPESC

Sequences:

>Translated_296_residues
MAPAASADPPSREGRECRDGDSTVQSLTRALQLIELLSDDDEGYRLVDLAARSGLSTSTVHRLLTTLEQRQFVQFDRERS
LWYVGVRCFSVGAAFARRRRISELALPVMQRLSALSGETVNLGVASDGQIVFACQAEGPHGSRALARPGQRAPLHGTAMG
QAVLAAKSEPDVLQYLRTFGLPRLTNNTIARPSRLHETLAEVRRRGYAVDDEQNAVGLCCIAAAICDEHGRPFAAVSLAG
SSQRIVRARFDELGAWVRWAATEISAAYGGRCTRVPCPMPVPAASLPFHAGAPESC
>Mature_295_residues
APAASADPPSREGRECRDGDSTVQSLTRALQLIELLSDDDEGYRLVDLAARSGLSTSTVHRLLTTLEQRQFVQFDRERSL
WYVGVRCFSVGAAFARRRRISELALPVMQRLSALSGETVNLGVASDGQIVFACQAEGPHGSRALARPGQRAPLHGTAMGQ
AVLAAKSEPDVLQYLRTFGLPRLTNNTIARPSRLHETLAEVRRRGYAVDDEQNAVGLCCIAAAICDEHGRPFAAVSLAGS
SQRIVRARFDELGAWVRWAATEISAAYGGRCTRVPCPMPVPAASLPFHAGAPESC

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=267, Percent_Identity=40.4494382022472, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1786715, Length=245, Percent_Identity=35.5102040816327, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=28.8617886178862, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788131, Length=247, Percent_Identity=29.9595141700405, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789999, Length=252, Percent_Identity=32.5396825396825, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1790752, Length=226, Percent_Identity=25.2212389380531, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788581, Length=256, Percent_Identity=25.390625, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31953; Mature: 31821

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAASADPPSREGRECRDGDSTVQSLTRALQLIELLSDDDEGYRLVDLAARSGLSTSTV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHH
HRLLTTLEQRQFVQFDRERSLWYVGVRCFSVGAAFARRRRISELALPVMQRLSALSGETV
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NLGVASDGQIVFACQAEGPHGSRALARPGQRAPLHGTAMGQAVLAAKSEPDVLQYLRTFG
EEEECCCCCEEEEEECCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHC
LPRLTNNTIARPSRLHETLAEVRRRGYAVDDEQNAVGLCCIAAAICDEHGRPFAAVSLAG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
SSQRIVRARFDELGAWVRWAATEISAAYGGRCTRVPCPMPVPAASLPFHAGAPESC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
APAASADPPSREGRECRDGDSTVQSLTRALQLIELLSDDDEGYRLVDLAARSGLSTSTV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHH
HRLLTTLEQRQFVQFDRERSLWYVGVRCFSVGAAFARRRRISELALPVMQRLSALSGETV
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NLGVASDGQIVFACQAEGPHGSRALARPGQRAPLHGTAMGQAVLAAKSEPDVLQYLRTFG
EEEECCCCCEEEEEECCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHC
LPRLTNNTIARPSRLHETLAEVRRRGYAVDDEQNAVGLCCIAAAICDEHGRPFAAVSLAG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
SSQRIVRARFDELGAWVRWAATEISAAYGGRCTRVPCPMPVPAASLPFHAGAPESC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]