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The map label for this gene is nylB' [H]

Identifier: 116694817

GI number: 116694817

Start: 987893

End: 989248

Strand: Direct

Name: nylB' [H]

Synonym: H16_B0871

Alternate gene names: 116694817

Gene position: 987893-989248 (Clockwise)

Preceding gene: 116694815

Following gene: 116694819

Centisome position: 33.92

GC content: 70.43

Gene sequence:

>1356_bases
ATGCAAGCGCAAACAGTGTCGCGCAGGACGGGATTCGGACGCAGTCGGGTGTGGCTGGCCGTGGCGCTGCTGGTGGCGCC
GATGAGCGTTGCGCTGGCGCAGGGCACCGGCCAGCCTTTGGCCGTTGCCGAGGACGAGGTCATGCAGGGCTTTCCGCCGC
CGCCCGACAAGCAGGTCAGCCGCGGCAGCGGGCTGCGTCCGCCGTATATGCGCTGGGCGTTCCGGCATGCGCGCGAGATG
TCGCCGACGGCGGGCATCCGGCATGCCAGCCAGCCGCTGGCCCTGGCCGGCCAGCCCGGCACGGAACTGGACGGCACCAC
CTTTGCCGTGGCCGGCAAGACGGTCCGACTGGCCGACTACCTGCGCGACACCCATACCGACGGCTTTATCGTGCTGCACC
AGGGCAAGGTCGTCTATGAGCGCTACCTCGCGGGCTTTGGTCCTTACCAGCCGCATATCTGGGCCTCGATGACCAAGTCC
GTCACCGGGCTGCTGGCCGCCATGCTGGTCGAGGAGGGCAAGCTCGATCCGCAGGCCCGGCTGGCGCAGTATGTGCCGGA
ACTGGCCGGCAATCCGTTCGGCGAGGCCACGGTACAGCAGAACCTGGATATGGAGGTGCCGGTGGGCTACCCGGAGGGGC
TGCCGCCGGACCTGGCGCTGTTCGGGGCGGTGGGGATCGTGCCGCGCAAGGCGGAGGCGCCGGACACCATCTATGACTTC
CTCAAGGTCGTCCACGCCACCGGCGGGCGCGACGATGGCGGCGTCTGGTACTACCAGAACGGCTCGCCGGAAGCGGTAGC
ATGGGCGCTGCGACGCATCACCGGCAAGAGCTGGGCGCAACTGGTGACCGAGCGGCTGTGGGCCCGCTTTGCCGACGATG
ATGCCTACACCCAGGTGGACCGCCAGGGCACGGAAATGGCCAGCGGTGGCATGAATTCCACGCTGCGCGACGCCGCCCGC
TTTGCCGAGGCCGTGCGCCGGGCGGCCGCGGGCGACGCCTCGGGCGGGATCTCGCCGGCGGCGGTGCGCATTGCGCTGCA
GCCGGCCGGCAACCAGGCCCGATTTGCGCGCGGCAACACCATGCCGGGCCGCGACGGCTATGGCTACCGCAACTACTGGT
TCCAGCGCAACGACGGCGACGGCAGCATCGAGGCCAGCGGGCGCTTCGGACAGAAGATCTATATCAATCCGGCCAGGGGG
CTGACGGTGGTGAAGTTCTCCGCCAGCCCGGACGGCGCCGCGCGTGCGACCAGCGCAGCCGCGGTGCGCAAGCGCGACGA
TCCGGCCCGGGCGCTGGAATCCGCCGAGGCCATGGTGGCCGCTGCGCTCGCGATCCAGCGCGCGGTCAGCCGCTGA

Upstream 100 bases:

>100_bases
TCCAAGATGGCGCCCGCGGCCTGCACCATGCGGGCTTGCACCTCATGCATACAAGGCGGCGCACCGTGCGTGCAACCGCC
TGGACAGGAATGGAGACAGC

Downstream 100 bases:

>100_bases
GCCACGCCGGCAGAGGCCGGCAGAGTCTCCCGATCTGGACTAGGCCGGCGTTTCCGCCGGCACCTGCGGTGCCCCCGCCG
CCCGCCGCAGGCAATCCAGC

Product: Beta-lactamase class C or other penicillin-binding protein

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQAQTVSRRTGFGRSRVWLAVALLVAPMSVALAQGTGQPLAVAEDEVMQGFPPPPDKQVSRGSGLRPPYMRWAFRHAREM
SPTAGIRHASQPLALAGQPGTELDGTTFAVAGKTVRLADYLRDTHTDGFIVLHQGKVVYERYLAGFGPYQPHIWASMTKS
VTGLLAAMLVEEGKLDPQARLAQYVPELAGNPFGEATVQQNLDMEVPVGYPEGLPPDLALFGAVGIVPRKAEAPDTIYDF
LKVVHATGGRDDGGVWYYQNGSPEAVAWALRRITGKSWAQLVTERLWARFADDDAYTQVDRQGTEMASGGMNSTLRDAAR
FAEAVRRAAAGDASGGISPAAVRIALQPAGNQARFARGNTMPGRDGYGYRNYWFQRNDGDGSIEASGRFGQKIYINPARG
LTVVKFSASPDGAARATSAAAVRKRDDPARALESAEAMVAAALAIQRAVSR

Sequences:

>Translated_451_residues
MQAQTVSRRTGFGRSRVWLAVALLVAPMSVALAQGTGQPLAVAEDEVMQGFPPPPDKQVSRGSGLRPPYMRWAFRHAREM
SPTAGIRHASQPLALAGQPGTELDGTTFAVAGKTVRLADYLRDTHTDGFIVLHQGKVVYERYLAGFGPYQPHIWASMTKS
VTGLLAAMLVEEGKLDPQARLAQYVPELAGNPFGEATVQQNLDMEVPVGYPEGLPPDLALFGAVGIVPRKAEAPDTIYDF
LKVVHATGGRDDGGVWYYQNGSPEAVAWALRRITGKSWAQLVTERLWARFADDDAYTQVDRQGTEMASGGMNSTLRDAAR
FAEAVRRAAAGDASGGISPAAVRIALQPAGNQARFARGNTMPGRDGYGYRNYWFQRNDGDGSIEASGRFGQKIYINPARG
LTVVKFSASPDGAARATSAAAVRKRDDPARALESAEAMVAAALAIQRAVSR
>Mature_451_residues
MQAQTVSRRTGFGRSRVWLAVALLVAPMSVALAQGTGQPLAVAEDEVMQGFPPPPDKQVSRGSGLRPPYMRWAFRHAREM
SPTAGIRHASQPLALAGQPGTELDGTTFAVAGKTVRLADYLRDTHTDGFIVLHQGKVVYERYLAGFGPYQPHIWASMTKS
VTGLLAAMLVEEGKLDPQARLAQYVPELAGNPFGEATVQQNLDMEVPVGYPEGLPPDLALFGAVGIVPRKAEAPDTIYDF
LKVVHATGGRDDGGVWYYQNGSPEAVAWALRRITGKSWAQLVTERLWARFADDDAYTQVDRQGTEMASGGMNSTLRDAAR
FAEAVRRAAAGDASGGISPAAVRIALQPAGNQARFARGNTMPGRDGYGYRNYWFQRNDGDGSIEASGRFGQKIYINPARG
LTVVKFSASPDGAARATSAAAVRKRDDPARALESAEAMVAAALAIQRAVSR

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 48430; Mature: 48430

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQTVSRRTGFGRSRVWLAVALLVAPMSVALAQGTGQPLAVAEDEVMQGFPPPPDKQVS
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHCCCCCCCCHHHC
RGSGLRPPYMRWAFRHAREMSPTAGIRHASQPLALAGQPGTELDGTTFAVAGKTVRLADY
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCHHHHHH
LRDTHTDGFIVLHQGKVVYERYLAGFGPYQPHIWASMTKSVTGLLAAMLVEEGKLDPQAR
HHHCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LAQYVPELAGNPFGEATVQQNLDMEVPVGYPEGLPPDLALFGAVGIVPRKAEAPDTIYDF
HHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
LKVVHATGGRDDGGVWYYQNGSPEAVAWALRRITGKSWAQLVTERLWARFADDDAYTQVD
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHH
RQGTEMASGGMNSTLRDAARFAEAVRRAAAGDASGGISPAAVRIALQPAGNQARFARGNT
HCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEECCCCC
MPGRDGYGYRNYWFQRNDGDGSIEASGRFGQKIYINPARGLTVVKFSASPDGAARATSAA
CCCCCCCCCCCCCEEECCCCCCEEECCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHH
AVRKRDDPARALESAEAMVAAALAIQRAVSR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQAQTVSRRTGFGRSRVWLAVALLVAPMSVALAQGTGQPLAVAEDEVMQGFPPPPDKQVS
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHCCCCCCCCHHHC
RGSGLRPPYMRWAFRHAREMSPTAGIRHASQPLALAGQPGTELDGTTFAVAGKTVRLADY
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCHHHHHH
LRDTHTDGFIVLHQGKVVYERYLAGFGPYQPHIWASMTKSVTGLLAAMLVEEGKLDPQAR
HHHCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LAQYVPELAGNPFGEATVQQNLDMEVPVGYPEGLPPDLALFGAVGIVPRKAEAPDTIYDF
HHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
LKVVHATGGRDDGGVWYYQNGSPEAVAWALRRITGKSWAQLVTERLWARFADDDAYTQVD
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHH
RQGTEMASGGMNSTLRDAARFAEAVRRAAAGDASGGISPAAVRIALQPAGNQARFARGNT
HCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEECCCCC
MPGRDGYGYRNYWFQRNDGDGSIEASGRFGQKIYINPARGLTVVKFSASPDGAARATSAA
CCCCCCCCCCCCCEEECCCCCCEEECCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHH
AVRKRDDPARALESAEAMVAAALAIQRAVSR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]