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The map label for this gene is hcaE [H]

Identifier: 116694623

GI number: 116694623

Start: 748214

End: 749314

Strand: Direct

Name: hcaE [H]

Synonym: H16_B0672

Alternate gene names: 116694623

Gene position: 748214-749314 (Clockwise)

Preceding gene: 116694622

Following gene: 116694624

Centisome position: 25.69

GC content: 64.76

Gene sequence:

>1101_bases
ATGACCGCTGACGGCAACCAGACTGGCTACAAGACCGGCTACAAGATCGAATCACGCCCGCTCGAGGCCCGCTACGCGCG
CGGCTGGCATTGCCTCGGCCTGGCCGATGGCTACCGCGACGGCGTGCCGCACACGCTGAATATCTTCGGCCGGCGCCTGG
CGGCGTTTGCCGACTCGACCGGCGCGATTCGCGTGATCGACGGCTACTGTCCGCACATGGGCGCAGACCTGAGCACCGGC
ACCGTGCAGGGCGACAACCTGGTCTGCCCGTTCCACGGCTGGCAGTGGGGCGGCGACGGCGGCTGCAAGTCCATTCCCTA
CTGCAAGCGGGTGCCGCCCAAGGCCCGCGTCGGCGCCTGGGAGACCTGCGAGCAGAACCACCTGCTCTTCATCTGGCACG
ATCCCGAAGGCCGCCCGGCACCCCTCGAGGTGGCGATTCCGCGCATCGATGCCTGCTTCTCTTCGGATTGGTCGGACTGG
GCCATCGACGAGATGCTGATCCGCACCAATTGCCGCGAGCTGGTCGACAATATCTCCGACATGGCGCATTTCGGCACGGT
CCATGGCGCGCCGGTGGACTATTTTGCCAACCTGTTCGAAGACCACAAGGCCACGCAGCTGATGATCGGGCGCAGTGCCC
GGCTCTCGGGCGATGCGCAGCTGACGGCGCTGTCCACGTACTTCGGGCCCGCATACCACATCACCGAGATGATCGGACAG
ATGGGCGACCAGCAGATCCACTCGATCCTGCTCAACTGCCATGTGCCGATCGACCTCAACAGCTTCGCGCTGCGCTACGG
CGTGTTGGTGAAGAAGATCCCGGGGCTGTCCGAGGAACAGAACCGTGCCATGGCGCAAGCCTATGTGGAGCAGGCCAAAC
AGGCCTTCTACGAGGACGTGGCGATCTGGGACAGCAAGATCCGCATCGACAACCCGTTGCTGTGCGAAGGCGACGGGCCC
CTCTACCAGATGCGTGACTGGTACCAGCAGTTCTACACCGACGTGGAGCAGGTGCGGCCGTCCAGCGTGGCACGGCGCAT
CTTTGAGCTGAACCCGGGCAATATCGAACCGCCGGCGCTGCGGCATGTGTTCGAGGCCTGA

Upstream 100 bases:

>100_bases
GACGAAGATTGCCGCGCTGCAGTAGTCCGATGGGACGATGAGCCGCCGGCCAGACGCTCTTATCTTGAAGGTCCCACGGG
AATTCAAGGAGACAGACGCC

Downstream 100 bases:

>100_bases
TGGCAACCTCCACGGAACCTCGCACCATGAAAACCGTCGTCATTACCGGAGCCGCGTCCGGTATGGGCGCGGCCGTGCGC
CAGCGCATGCTCGCCGACGG

Product: ring-hydroxylating dioxygenase

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTADGNQTGYKTGYKIESRPLEARYARGWHCLGLADGYRDGVPHTLNIFGRRLAAFADSTGAIRVIDGYCPHMGADLSTG
TVQGDNLVCPFHGWQWGGDGGCKSIPYCKRVPPKARVGAWETCEQNHLLFIWHDPEGRPAPLEVAIPRIDACFSSDWSDW
AIDEMLIRTNCRELVDNISDMAHFGTVHGAPVDYFANLFEDHKATQLMIGRSARLSGDAQLTALSTYFGPAYHITEMIGQ
MGDQQIHSILLNCHVPIDLNSFALRYGVLVKKIPGLSEEQNRAMAQAYVEQAKQAFYEDVAIWDSKIRIDNPLLCEGDGP
LYQMRDWYQQFYTDVEQVRPSSVARRIFELNPGNIEPPALRHVFEA

Sequences:

>Translated_366_residues
MTADGNQTGYKTGYKIESRPLEARYARGWHCLGLADGYRDGVPHTLNIFGRRLAAFADSTGAIRVIDGYCPHMGADLSTG
TVQGDNLVCPFHGWQWGGDGGCKSIPYCKRVPPKARVGAWETCEQNHLLFIWHDPEGRPAPLEVAIPRIDACFSSDWSDW
AIDEMLIRTNCRELVDNISDMAHFGTVHGAPVDYFANLFEDHKATQLMIGRSARLSGDAQLTALSTYFGPAYHITEMIGQ
MGDQQIHSILLNCHVPIDLNSFALRYGVLVKKIPGLSEEQNRAMAQAYVEQAKQAFYEDVAIWDSKIRIDNPLLCEGDGP
LYQMRDWYQQFYTDVEQVRPSSVARRIFELNPGNIEPPALRHVFEA
>Mature_365_residues
TADGNQTGYKTGYKIESRPLEARYARGWHCLGLADGYRDGVPHTLNIFGRRLAAFADSTGAIRVIDGYCPHMGADLSTGT
VQGDNLVCPFHGWQWGGDGGCKSIPYCKRVPPKARVGAWETCEQNHLLFIWHDPEGRPAPLEVAIPRIDACFSSDWSDWA
IDEMLIRTNCRELVDNISDMAHFGTVHGAPVDYFANLFEDHKATQLMIGRSARLSGDAQLTALSTYFGPAYHITEMIGQM
GDQQIHSILLNCHVPIDLNSFALRYGVLVKKIPGLSEEQNRAMAQAYVEQAKQAFYEDVAIWDSKIRIDNPLLCEGDGPL
YQMRDWYQQFYTDVEQVRPSSVARRIFELNPGNIEPPALRHVFEA

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=341, Percent_Identity=24.633431085044, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161085896, Length=339, Percent_Identity=24.188790560472, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: NA

Molecular weight: Translated: 41041; Mature: 40910

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADGNQTGYKTGYKIESRPLEARYARGWHCLGLADGYRDGVPHTLNIFGRRLAAFADST
CCCCCCCCCCCCCCEECCCCCHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
GAIRVIDGYCPHMGADLSTGTVQGDNLVCPFHGWQWGGDGGCKSIPYCKRVPPKARVGAW
CCEEEEECCCCCCCCCCCCCEECCCCEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
ETCEQNHLLFIWHDPEGRPAPLEVAIPRIDACFSSDWSDWAIDEMLIRTNCRELVDNISD
CCCCCCCEEEEEECCCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MAHFGTVHGAPVDYFANLFEDHKATQLMIGRSARLSGDAQLTALSTYFGPAYHITEMIGQ
HHHHCCCCCCCHHHHHHHHHHCCHHEEEECCCCCCCCCCEEEEHHHHCCCHHHHHHHHHH
MGDQQIHSILLNCHVPIDLNSFALRYGVLVKKIPGLSEEQNRAMAQAYVEQAKQAFYEDV
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AIWDSKIRIDNPLLCEGDGPLYQMRDWYQQFYTDVEQVRPSSVARRIFELNPGNIEPPAL
HHCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHH
RHVFEA
HHHHCC
>Mature Secondary Structure 
TADGNQTGYKTGYKIESRPLEARYARGWHCLGLADGYRDGVPHTLNIFGRRLAAFADST
CCCCCCCCCCCCCEECCCCCHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
GAIRVIDGYCPHMGADLSTGTVQGDNLVCPFHGWQWGGDGGCKSIPYCKRVPPKARVGAW
CCEEEEECCCCCCCCCCCCCEECCCCEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
ETCEQNHLLFIWHDPEGRPAPLEVAIPRIDACFSSDWSDWAIDEMLIRTNCRELVDNISD
CCCCCCCEEEEEECCCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MAHFGTVHGAPVDYFANLFEDHKATQLMIGRSARLSGDAQLTALSTYFGPAYHITEMIGQ
HHHHCCCCCCCHHHHHHHHHHCCHHEEEECCCCCCCCCCEEEEHHHHCCCHHHHHHHHHH
MGDQQIHSILLNCHVPIDLNSFALRYGVLVKKIPGLSEEQNRAMAQAYVEQAKQAFYEDV
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AIWDSKIRIDNPLLCEGDGPLYQMRDWYQQFYTDVEQVRPSSVARRIFELNPGNIEPPAL
HHCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHH
RHVFEA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA