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The map label for this gene is fadH [C]

Identifier: 116694622

GI number: 116694622

Start: 746897

End: 748138

Strand: Direct

Name: fadH [C]

Synonym: H16_B0671

Alternate gene names: 116694622

Gene position: 746897-748138 (Clockwise)

Preceding gene: 116694621

Following gene: 116694623

Centisome position: 25.64

GC content: 70.05

Gene sequence:

>1242_bases
ATGCACGATCACCAGGCTTACGCGGCGGCGGCATTCACACCGGTCCGCATCGGCCCGCTCACGCTGCGCAATCGCATTAT
CAAGGCCGGCGCCAACGAGGGCATGACGCCGCAGGGCCTGCCGACCGAGGCGCTGGTCAGGCACCACCGCGAGCTTGCCG
CGGGCGGCGTGGGCATGACCACGGTGGCGTATGCGGCGGTGGCCGACGACGGCCTGACTTTTTCGCACCAGCTCAGTATG
CGCCCGGAGCAGGTTGCGCACCTGCGCGTGCTGACCGATGCCGTGCATCGCGAAGGCGCGGCGGCCTGCCTGCAGATCAC
CCATGCGGGCTCGTTCACCACCATGCGCCATGGCGGCAGCCGCGCGCCGGGCAGTGCTTCATCCGGACTCAATGCCTTTG
GCATGCTGCATGGCGTGTATTTCCAGCGTGCCATGCGCGAGGCGGACATGGCGCGCGTTGCCGGCCAGTTTGCCGCCGCG
GCGCGGCTGGCGCGCGAAGCCGGCTTCGACGCGGTGGAAATCCACATGGGGCACGGCTACCTGCTCAACCAGTTCCTGTC
GCCACTGAGCAACCGTCGGCGCGATGCGTTCGGCGGCAGCGTGGAGAACCGCACCCGCTTTCCGGCCGCGGTACTGCGCC
AGGTGAAGGACGCGGTCGGCGCCGAGCTGGCGGTCTGCTGCAAGCTGAGCGTCAGCGACGGCGTGCCGGGCGGCAACGAA
CCCGCGGATTCCGCCGTGAGCGCGCGCCTGCTGGAGGCGGAAGGCGCCGACCTGCTGACCCTCAGCGGCGGGCGCAACGT
CGAGAGCCCGTGGGCGCTGTTCGGCAGCCCCATGCCCACGGCGCAGATGAAGGCCGCCGCACCGACCGCGGTGGCGCGCT
TCGGCATCACCATGCTGGAGAAGCGCACGCCGCGCGCGCTGGCCTTTCGCGAGCTGTACTTCCTGGAGGCGTCGCGCGTG
GTGCGGCGGGCGGTGCGCATGCCGCTGGCCTATATCGGCGGCGTCAAGTCGATCGGCAATGTCGCGCAGCTGATGGAAGA
GGGCTTCGACTGCATCGCGCTGGCGCGCGCGCTGATCCACGATCCCGCACTGGTCGCCAACTGGCGCAGCGGCGCGGTGG
CGCATTCGGCCTGTGACAGCTGCAATGGCTGCGTGGCGCGGATCTACGATCCGGCCGGTGTCAGTTGCGTGCATGGCCCG
GGCAATGACCCGGCGCTGACGAAGATTGCCGCGCTGCAGTAG

Upstream 100 bases:

>100_bases
CCGCCGGTGGTGCCGGTGGCGCCGCGCGCGGCGAGACGGTGCCGCCGGCGCACGCCCTGAAATCCGACGCGGTGTAAGGC
CGATGACCCGGAGAGACAGC

Downstream 100 bases:

>100_bases
TCCGATGGGACGATGAGCCGCCGGCCAGACGCTCTTATCTTGAAGGTCCCACGGGAATTCAAGGAGACAGACGCCATGAC
CGCTGACGGCAACCAGACTG

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MHDHQAYAAAAFTPVRIGPLTLRNRIIKAGANEGMTPQGLPTEALVRHHRELAAGGVGMTTVAYAAVADDGLTFSHQLSM
RPEQVAHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGSRAPGSASSGLNAFGMLHGVYFQRAMREADMARVAGQFAAA
ARLAREAGFDAVEIHMGHGYLLNQFLSPLSNRRRDAFGGSVENRTRFPAAVLRQVKDAVGAELAVCCKLSVSDGVPGGNE
PADSAVSARLLEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPTAVARFGITMLEKRTPRALAFRELYFLEASRV
VRRAVRMPLAYIGGVKSIGNVAQLMEEGFDCIALARALIHDPALVANWRSGAVAHSACDSCNGCVARIYDPAGVSCVHGP
GNDPALTKIAALQ

Sequences:

>Translated_413_residues
MHDHQAYAAAAFTPVRIGPLTLRNRIIKAGANEGMTPQGLPTEALVRHHRELAAGGVGMTTVAYAAVADDGLTFSHQLSM
RPEQVAHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGSRAPGSASSGLNAFGMLHGVYFQRAMREADMARVAGQFAAA
ARLAREAGFDAVEIHMGHGYLLNQFLSPLSNRRRDAFGGSVENRTRFPAAVLRQVKDAVGAELAVCCKLSVSDGVPGGNE
PADSAVSARLLEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPTAVARFGITMLEKRTPRALAFRELYFLEASRV
VRRAVRMPLAYIGGVKSIGNVAQLMEEGFDCIALARALIHDPALVANWRSGAVAHSACDSCNGCVARIYDPAGVSCVHGP
GNDPALTKIAALQ
>Mature_413_residues
MHDHQAYAAAAFTPVRIGPLTLRNRIIKAGANEGMTPQGLPTEALVRHHRELAAGGVGMTTVAYAAVADDGLTFSHQLSM
RPEQVAHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGSRAPGSASSGLNAFGMLHGVYFQRAMREADMARVAGQFAAA
ARLAREAGFDAVEIHMGHGYLLNQFLSPLSNRRRDAFGGSVENRTRFPAAVLRQVKDAVGAELAVCCKLSVSDGVPGGNE
PADSAVSARLLEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPTAVARFGITMLEKRTPRALAFRELYFLEASRV
VRRAVRMPLAYIGGVKSIGNVAQLMEEGFDCIALARALIHDPALVANWRSGAVAHSACDSCNGCVARIYDPAGVSCVHGP
GNDPALTKIAALQ

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=396, Percent_Identity=26.7676767676768, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787939, Length=279, Percent_Identity=30.4659498207885, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=391, Percent_Identity=25.0639386189258, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=399, Percent_Identity=25.062656641604, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=368, Percent_Identity=25.2717391304348, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=271, Percent_Identity=28.4132841328413, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=364, Percent_Identity=24.1758241758242, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=278, Percent_Identity=29.4964028776978, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=387, Percent_Identity=23.2558139534884, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321973, Length=179, Percent_Identity=34.0782122905028, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=104, Percent_Identity=38.4615384615385, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 43580; Mature: 43580

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDHQAYAAAAFTPVRIGPLTLRNRIIKAGANEGMTPQGLPTEALVRHHRELAAGGVGMT
CCCCHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
TVAYAAVADDGLTFSHQLSMRPEQVAHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGS
HHHHHHHHCCCCEEEHCCCCCHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEECCCCC
RAPGSASSGLNAFGMLHGVYFQRAMREADMARVAGQFAAAARLAREAGFDAVEIHMGHGY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
LLNQFLSPLSNRRRDAFGGSVENRTRFPAAVLRQVKDAVGAELAVCCKLSVSDGVPGGNE
HHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
PADSAVSARLLEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPTAVARFGITMLE
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCHHHHHCCHHHHHHHHHHHHH
KRTPRALAFRELYFLEASRVVRRAVRMPLAYIGGVKSIGNVAQLMEEGFDCIALARALIH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHC
DPALVANWRSGAVAHSACDSCNGCVARIYDPAGVSCVHGPGNDPALTKIAALQ
CCHHEECCCCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MHDHQAYAAAAFTPVRIGPLTLRNRIIKAGANEGMTPQGLPTEALVRHHRELAAGGVGMT
CCCCHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
TVAYAAVADDGLTFSHQLSMRPEQVAHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGS
HHHHHHHHCCCCEEEHCCCCCHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEECCCCC
RAPGSASSGLNAFGMLHGVYFQRAMREADMARVAGQFAAAARLAREAGFDAVEIHMGHGY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
LLNQFLSPLSNRRRDAFGGSVENRTRFPAAVLRQVKDAVGAELAVCCKLSVSDGVPGGNE
HHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
PADSAVSARLLEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPTAVARFGITMLE
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCHHHHHCCHHHHHHHHHHHHH
KRTPRALAFRELYFLEASRVVRRAVRMPLAYIGGVKSIGNVAQLMEEGFDCIALARALIH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHC
DPALVANWRSGAVAHSACDSCNGCVARIYDPAGVSCVHGPGNDPALTKIAALQ
CCHHEECCCCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]