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The map label for this gene is rfbJ [H]

Identifier: 113475904

GI number: 113475904

Start: 3522037

End: 3522942

Strand: Direct

Name: rfbJ [H]

Synonym: Tery_2264

Alternate gene names: 113475904

Gene position: 3522037-3522942 (Clockwise)

Preceding gene: 113475903

Following gene: 113475905

Centisome position: 45.45

GC content: 35.43

Gene sequence:

>906_bases
ATGACACAAACAATTTTAGTTACTGGGGCTACAGGATTTGTGGGAAGTCATTTGGTTAGGCAACTCCTGAATAAAGGCAA
TCGAGTTATTATTCTCAAGCGTAGTTTTTCTGAAACTAAAAGGATTGAGAATTGCCTGTCACAGATAACTGCTTATGACA
TAGATCGGTGCAACATAGAAAAACCTTTTCAAGACTTTGGAAAAATTGATGCAGTAGTTCACACAGCAACGGCCACTGGT
CGCCGTCAAGAGAGTCTTATAGAAATATTTGAGGCTAATACTGCATTTCCTTTAAAATTGTTGAAAACAGCCAAAGATTT
CCAGACCAAGACTTTTATTAATACGGATACTTATGTTAATAAAAGCAATATATTATATCAAGGATTAGAAAGCTACTCTC
TTTCAAAAAAACATTTCCAGCAGTGGGGAAAACAAATTGCTCTAATTCATCAGATAAAGTTTTTGAATGTTAGATTAGAA
TACGTTTTTGGCCAAGGGGATTATCGTAACAAACTAATATCTTACGCGAGCCAGCAGTTTTTAAGCAATGCCTCTGAGTT
AAAATTCACTTCGGGGGAACAGCAACGAGATTTTATTCATGTTGAAGATGTAGTTTTGGCCTATCTTTTGCTGCTAGAAA
AAGAATCGAAACCCTCACAATATTATCAAGAATATGAAGTAGGAACAGGTAAAGCAACTTCATTGCGACAACTTCTAGAA
ATGTTGAAAGAATTAATGCAAGTTCAAACAGAATTAAAATTTGGAGCTTTACCTCAACGTCGGGGTGAAATTATGTTTTC
TCAGGCTGACACTAAGACCATTGAAGAAATTGGTTGGTATCCGGCCAAAAGTTTAAAAGAACGTTTGATGCAAACCATTG
AAGGTGAAAAAAAACTCAAAGATTAA

Upstream 100 bases:

>100_bases
AATCCACCAGTTGTGGTTGATGGCAGAAGAATGTTGGGTAAACAGAGTGTTTCTATCTATGAAGGAATAGGTTGGTCCTC
CAAATCAAAGTATTGAAAAC

Downstream 100 bases:

>100_bases
CTAGGTTGGCTCAAGACTATTTTTGACACATCAGCACATTGCTGATAGAATACTAAAATTGTTTTACTTATATTATATTA
TTATATTCAGGACTTATGCA

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: O4 antigen [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTQTILVTGATGFVGSHLVRQLLNKGNRVIILKRSFSETKRIENCLSQITAYDIDRCNIEKPFQDFGKIDAVVHTATATG
RRQESLIEIFEANTAFPLKLLKTAKDFQTKTFINTDTYVNKSNILYQGLESYSLSKKHFQQWGKQIALIHQIKFLNVRLE
YVFGQGDYRNKLISYASQQFLSNASELKFTSGEQQRDFIHVEDVVLAYLLLLEKESKPSQYYQEYEVGTGKATSLRQLLE
MLKELMQVQTELKFGALPQRRGEIMFSQADTKTIEEIGWYPAKSLKERLMQTIEGEKKLKD

Sequences:

>Translated_301_residues
MTQTILVTGATGFVGSHLVRQLLNKGNRVIILKRSFSETKRIENCLSQITAYDIDRCNIEKPFQDFGKIDAVVHTATATG
RRQESLIEIFEANTAFPLKLLKTAKDFQTKTFINTDTYVNKSNILYQGLESYSLSKKHFQQWGKQIALIHQIKFLNVRLE
YVFGQGDYRNKLISYASQQFLSNASELKFTSGEQQRDFIHVEDVVLAYLLLLEKESKPSQYYQEYEVGTGKATSLRQLLE
MLKELMQVQTELKFGALPQRRGEIMFSQADTKTIEEIGWYPAKSLKERLMQTIEGEKKLKD
>Mature_300_residues
TQTILVTGATGFVGSHLVRQLLNKGNRVIILKRSFSETKRIENCLSQITAYDIDRCNIEKPFQDFGKIDAVVHTATATGR
RQESLIEIFEANTAFPLKLLKTAKDFQTKTFINTDTYVNKSNILYQGLESYSLSKKHFQQWGKQIALIHQIKFLNVRLEY
VFGQGDYRNKLISYASQQFLSNASELKFTSGEQQRDFIHVEDVVLAYLLLLEKESKPSQYYQEYEVGTGKATSLRQLLEM
LKELMQVQTELKFGALPQRRGEIMFSQADTKTIEEIGWYPAKSLKERLMQTIEGEKKLKD

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI21356223, Length=311, Percent_Identity=24.1157556270096, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 34677; Mature: 34546

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTILVTGATGFVGSHLVRQLLNKGNRVIILKRSFSETKRIENCLSQITAYDIDRCNIE
CCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
KPFQDFGKIDAVVHTATATGRRQESLIEIFEANTAFPLKLLKTAKDFQTKTFINTDTYVN
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCEEC
KSNILYQGLESYSLSKKHFQQWGKQIALIHQIKFLNVRLEYVFGQGDYRNKLISYASQQF
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHH
LSNASELKFTSGEQQRDFIHVEDVVLAYLLLLEKESKPSQYYQEYEVGTGKATSLRQLLE
HCCHHHCEECCCCCHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
MLKELMQVQTELKFGALPQRRGEIMFSQADTKTIEEIGWYPAKSLKERLMQTIEGEKKLK
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCHHHHCC
D
C
>Mature Secondary Structure 
TQTILVTGATGFVGSHLVRQLLNKGNRVIILKRSFSETKRIENCLSQITAYDIDRCNIE
CCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
KPFQDFGKIDAVVHTATATGRRQESLIEIFEANTAFPLKLLKTAKDFQTKTFINTDTYVN
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCEEC
KSNILYQGLESYSLSKKHFQQWGKQIALIHQIKFLNVRLEYVFGQGDYRNKLISYASQQF
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHH
LSNASELKFTSGEQQRDFIHVEDVVLAYLLLLEKESKPSQYYQEYEVGTGKATSLRQLLE
HCCHHHCEECCCCCHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
MLKELMQVQTELKFGALPQRRGEIMFSQADTKTIEEIGWYPAKSLKERLMQTIEGEKKLK
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHCHHHHCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793832; 1710759; 11677609 [H]