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The map label for this gene is fabI [H]

Identifier: 113475683

GI number: 113475683

Start: 3151814

End: 3152590

Strand: Direct

Name: fabI [H]

Synonym: Tery_2024

Alternate gene names: 113475683

Gene position: 3151814-3152590 (Clockwise)

Preceding gene: 113475679

Following gene: 113475685

Centisome position: 40.67

GC content: 39.38

Gene sequence:

>777_bases
ATGTTAAACTTAACTGGAAAAAATGCTCTGGTGACAGGGATTGCAAATAATCGTTCTATTGCTTGGGGTATTGCTCAACA
ACTTCATAAAGCCGGGGCTAATTTGGGGGTTACTTATCTACCAGATGATAAGGGTAAGTTTCAAAAGAAGGTTTGGGAGT
TAGTCGAACCTTTGAGTCCTTCTTTATATTTACCTTGTAATGTTCAAGATGACCAACAGGTTAAACAGACTTTTGCCCAA
GTAGGGGAAAAGTGGGGTAAGCTGGATATTTTAATTCATTGTTTGGCTTTTGCTGGTAAGGAGGAGTTGTCTGGGGATTT
TAGTAATACTACTCGTGAAGGGTTTACTCGTGCTTTGGAGATTAGTTCTTATTCTTTGATTGATTTAAGCTCAAAGGCTA
AGGAGTTGATGACTGAAGGAGGGAGTATTCTGACTCTGAGTTACTTGGGTGGGGTGCGAGTTGTTCGTAATTATAATGTT
ATGGGAATTGCTAAGTCTGCTTTGGAAATGAATGTGCGTTATTTAGCAGCAGAACTTGGGCCCAAAAATATTAGGATAAA
TGCTATTTCTGCTGGTCCAATTAGAACTTTGGCTTCTTCTGCTGTTGGTGGTATTTTGGATATGATTCATCATGTGGAAA
AAGTGGCTCCGTTGCGTCGTACGGTGACTCAAATTGAAATTGGTAATACTGCTGCTTTTTTATGTAGTGATCTGGCAAGT
GGAATTACTGGTCAGGTTGTCTATGTGGATGCTGGTTATGAAATTATGGGAATTTAG

Upstream 100 bases:

>100_bases
TATACATATAATAATCATACCATCAATCGGTAATACATCATGATCAAATTAAATTTGAAATTTTTGATCAAATTAAATAT
TAATAAAAATTAATGATATA

Downstream 100 bases:

>100_bases
ATATTGAACTCAAAAGTCAGCACATTTCTAGTCAGAAGTAAAGAAAGTATAGCTAAATTCAAAATTCAGAAGTAAACAAA
AGTAAGTAATAAAGTTTTCT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLNLTGKNALVTGIANNRSIAWGIAQQLHKAGANLGVTYLPDDKGKFQKKVWELVEPLSPSLYLPCNVQDDQQVKQTFAQ
VGEKWGKLDILIHCLAFAGKEELSGDFSNTTREGFTRALEISSYSLIDLSSKAKELMTEGGSILTLSYLGGVRVVRNYNV
MGIAKSALEMNVRYLAAELGPKNIRINAISAGPIRTLASSAVGGILDMIHHVEKVAPLRRTVTQIEIGNTAAFLCSDLAS
GITGQVVYVDAGYEIMGI

Sequences:

>Translated_258_residues
MLNLTGKNALVTGIANNRSIAWGIAQQLHKAGANLGVTYLPDDKGKFQKKVWELVEPLSPSLYLPCNVQDDQQVKQTFAQ
VGEKWGKLDILIHCLAFAGKEELSGDFSNTTREGFTRALEISSYSLIDLSSKAKELMTEGGSILTLSYLGGVRVVRNYNV
MGIAKSALEMNVRYLAAELGPKNIRINAISAGPIRTLASSAVGGILDMIHHVEKVAPLRRTVTQIEIGNTAAFLCSDLAS
GITGQVVYVDAGYEIMGI
>Mature_258_residues
MLNLTGKNALVTGIANNRSIAWGIAQQLHKAGANLGVTYLPDDKGKFQKKVWELVEPLSPSLYLPCNVQDDQQVKQTFAQ
VGEKWGKLDILIHCLAFAGKEELSGDFSNTTREGFTRALEISSYSLIDLSSKAKELMTEGGSILTLSYLGGVRVVRNYNV
MGIAKSALEMNVRYLAAELGPKNIRINAISAGPIRTLASSAVGGILDMIHHVEKVAPLRRTVTQIEIGNTAAFLCSDLAS
GITGQVVYVDAGYEIMGI

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=198, Percent_Identity=29.2929292929293, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI7705925, Length=257, Percent_Identity=27.6264591439689, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI32483357, Length=264, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=48.6166007905138, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI1789378, Length=246, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787905, Length=256, Percent_Identity=29.6875, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=28.3524904214559, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI2367365, Length=257, Percent_Identity=28.4046692607004, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=276, Percent_Identity=28.2608695652174, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=276, Percent_Identity=27.1739130434783, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21357041, Length=204, Percent_Identity=28.921568627451, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644337, Length=180, Percent_Identity=30, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571526, Length=223, Percent_Identity=27.8026905829596, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI23397609, Length=262, Percent_Identity=25.9541984732824, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27841; Mature: 27841

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLTGKNALVTGIANNRSIAWGIAQQLHKAGANLGVTYLPDDKGKFQKKVWELVEPLSP
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCC
SLYLPCNVQDDQQVKQTFAQVGEKWGKLDILIHCLAFAGKEELSGDFSNTTREGFTRALE
CEEEECCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHE
ISSYSLIDLSSKAKELMTEGGSILTLSYLGGVRVVRNYNVMGIAKSALEMNVRYLAAELG
ECCEEEEECHHHHHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
PKNIRINAISAGPIRTLASSAVGGILDMIHHVEKVAPLRRTVTQIEIGNTAAFLCSDLAS
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHC
GITGQVVYVDAGYEIMGI
CCCCEEEEEECCEEEECC
>Mature Secondary Structure
MLNLTGKNALVTGIANNRSIAWGIAQQLHKAGANLGVTYLPDDKGKFQKKVWELVEPLSP
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCC
SLYLPCNVQDDQQVKQTFAQVGEKWGKLDILIHCLAFAGKEELSGDFSNTTREGFTRALE
CEEEECCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHE
ISSYSLIDLSSKAKELMTEGGSILTLSYLGGVRVVRNYNVMGIAKSALEMNVRYLAAELG
ECCEEEEECHHHHHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
PKNIRINAISAGPIRTLASSAVGGILDMIHHVEKVAPLRRTVTQIEIGNTAAFLCSDLAS
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHC
GITGQVVYVDAGYEIMGI
CCCCEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]