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The map label for this gene is yibK [C]

Identifier: 113474137

GI number: 113474137

Start: 372181

End: 372642

Strand: Reverse

Name: yibK [C]

Synonym: Tery_0238

Alternate gene names: 113474137

Gene position: 372642-372181 (Counterclockwise)

Preceding gene: 113474139

Following gene: 113474136

Centisome position: 4.81

GC content: 37.45

Gene sequence:

>462_bases
ATGCCTAATATTGTCCTAGTCTATCCACAAATTCCCCAAAATACAGGTAATGTTGCTCGTACTTGTGCTGCTACCAAAAC
CGAATTACATCTAGTCGGACCTTTAGGTTTTGAAATTAGCGATCGCTATTTGAAACGTGCTGGTTTAGACTACTGGCCTT
ATGTCAAACTTAACTATCACAATGACTGGGCAGAGTTTAAAGTCTATCACCATAAACTTGGTGGAAGACTCATTGGCTTC
AGTAAGATGGGAAAATTAAATTATACCAAATTTCAATATCAAACAAATGATTGGCTATTATTTGGTTCCGAAACTAATGG
ACTACCAAAAGATGTCTTGAACTATTGTAGTGCAACTTTATATATACCAATGACTCAGCCAAACGTTCGTAGTTTAAATC
TTTCTGTAAGTGCTGCGTTAGGTTTGTTTGAGGCCAGGCGTCAATTGGGTTATCTGAGTTAG

Upstream 100 bases:

>100_bases
TTAAAGCGGCCCTAATTGATTCCCTGTCAAAATATATATCCATCAATAAACCTTTTTACTCGTTCTGATTTGATGGACGT
CAAAATATTTCATTCGTTTC

Downstream 100 bases:

>100_bases
AATCTTAATTTTATTACTCAAAAATTAAAATCCCTGTGGAGCTGTTGAGCAATTCTACAGATTTTTCTACACAGCTACCA
TAAGAACTGTTATGTGATCT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPNIVLVYPQIPQNTGNVARTCAATKTELHLVGPLGFEISDRYLKRAGLDYWPYVKLNYHNDWAEFKVYHHKLGGRLIGF
SKMGKLNYTKFQYQTNDWLLFGSETNGLPKDVLNYCSATLYIPMTQPNVRSLNLSVSAALGLFEARRQLGYLS

Sequences:

>Translated_153_residues
MPNIVLVYPQIPQNTGNVARTCAATKTELHLVGPLGFEISDRYLKRAGLDYWPYVKLNYHNDWAEFKVYHHKLGGRLIGF
SKMGKLNYTKFQYQTNDWLLFGSETNGLPKDVLNYCSATLYIPMTQPNVRSLNLSVSAALGLFEARRQLGYLS
>Mature_152_residues
PNIVLVYPQIPQNTGNVARTCAATKTELHLVGPLGFEISDRYLKRAGLDYWPYVKLNYHNDWAEFKVYHHKLGGRLIGFS
KMGKLNYTKFQYQTNDWLLFGSETNGLPKDVLNYCSATLYIPMTQPNVRSLNLSVSAALGLFEARRQLGYLS

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=41.8300653594771, Blast_Score=120, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17437; Mature: 17306

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNIVLVYPQIPQNTGNVARTCAATKTELHLVGPLGFEISDRYLKRAGLDYWPYVKLNYH
CCCEEEEECCCCCCCCCHHHHEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEC
NDWAEFKVYHHKLGGRLIGFSKMGKLNYTKFQYQTNDWLLFGSETNGLPKDVLNYCSATL
CCHHHHEEEEECCCCEEEEECCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHCCEEE
YIPMTQPNVRSLNLSVSAALGLFEARRQLGYLS
EEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNIVLVYPQIPQNTGNVARTCAATKTELHLVGPLGFEISDRYLKRAGLDYWPYVKLNYH
CCEEEEECCCCCCCCCHHHHEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEC
NDWAEFKVYHHKLGGRLIGFSKMGKLNYTKFQYQTNDWLLFGSETNGLPKDVLNYCSATL
CCHHHHEEEEECCCCEEEEECCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHCCEEE
YIPMTQPNVRSLNLSVSAALGLFEARRQLGYLS
EEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]