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The map label for this gene is trmE

Identifier: 110835613

GI number: 110835613

Start: 3115823

End: 3117193

Strand: Reverse

Name: trmE

Synonym: ABO_2752

Alternate gene names: 110835613

Gene position: 3117193-3115823 (Counterclockwise)

Preceding gene: 110835614

Following gene: 110835611

Centisome position: 99.91

GC content: 53.17

Gene sequence:

>1371_bases
ATGCTCACTTCGTCTCCAGATACCATTGTTGCCATTGCTACTGCTCCCGGGCGGGGCGGTGTCGGTGTGGTACGCCTTTC
CGGCCCGCAGGCGCTAAGCATAGCGTCATCCATTGTTGGTGAGCGGTTACTTTCCCCACGGTTGGCGCATTTTTGTCGCT
TTCGCAATCAGGATGGTGACGTTCTGGATGAAGGTTTGGTGATCAGCTTTCCCGCCCCTCACTCTTTTACCGGGGAAGAT
GTGGTCGAGTTGCAGGGGCACGGTGGCCCGGTAATTTTAGACATGTTGGTGGCCACATGTATTGAGGCGGGGGCTCGGCA
GGCCCGTGCCGGTGAATTTTCCCAGCGGGCTTTCCTTAACGACAAGATGGATCTAACCCAGGCGGAAGCTATCGCCGATT
TGATTGATGCGGGCACGGCTGCCTCCGCGAGGGCGGCGCTACATTCTTTACAAGGGGTTTTTTCTGCTGAAGTAAATCAA
CTGGTGGAAGCACTGATTGGCCTGCGCATCTATGTGGAGGCAGCCATCGATTTCCCGGAAGAAGAAATTGACTTCCTGTC
TGATGGTAAGGTCGCTAGTGATCTTAGGGCGGTACGCAAGCAGTGCCTGCGGGTGCTCGGTGAAGCCAATCAAGGACGGT
TGGTACGCGAAGGTATGACCTTGGTGATTGCTGGTAAACCGAACGCGGGAAAATCATCATTGATGAATGCTCTGGCCGGT
TTTGATGCGGCTATCGTCACTGATATTGCCGGCACCACTCGGGATGTACTGCGTGAGTCCATTCAGTTGGATGGTATGCC
CTTGAACCTGATTGATACCGCTGGGCTACGCGACAGTGCCGATGTGGTGGAACAGGAGGGTATCCGCCGCGCTTACGCTG
AAATGCGTAAGGCTGATCGTTTGTTGGTCATGGTGGATTCCCGTGATCCGCTAGACGATATTCACAATCTCCACGATCTG
TTGCCGCAAAGCCAACAGCAATTGGAAAATTTGGACTTACCGGTAACCGTAGTGCTCAATAAAGCCGATTTGTCAGGATT
ATCAACGGGTCCGCGTGCTGATGGGGCTTTTGTGATTTCCGCGGCCAATGGCGAGGGCTTGGATGCGTTGCGCGGCCATT
TGAAGCAGGTTGCGGGTTATCAGGGGGAAGGCCAAAGCCGTTTTTCTGCCCGTCGTCGCCATATTACCGCTCTTGAGAAA
GCGCTGATGGCATTGGAAAGTGGTGAGCAACAACTGCATGGTCAGGCTGCTGGAGAATTGTTAGCTGAAGACCTTCGTGC
TGCTCAGAAGGCACTAGAAGAGATCACCGGAGTATTTACGGCGGATGATCTTTTAGGTCGTATTTTTTCATCATTTTGTA
TCGGTAAATAA

Upstream 100 bases:

>100_bases
ACGTTGGCTGCAGAAAAGAGTAAAGCCGGTCTCACGACCGGCTTTACTCTTTTCTGGCCACGTCTAGCATCAAACGTCGA
GTGCTTTCTATAATCAGCCT

Downstream 100 bases:

>100_bases
TCAAGCAACGTTACCCGCTAACCATAGCGACGCTTTTTCTTGGGAGTAACTATGGGCTTGTTAGGCTTATTGCCATGAGA
GGGACGCGAGTAGCGAGGGG

Product: tRNA modification GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLTSSPDTIVAIATAPGRGGVGVVRLSGPQALSIASSIVGERLLSPRLAHFCRFRNQDGDVLDEGLVISFPAPHSFTGED
VVELQGHGGPVILDMLVATCIEAGARQARAGEFSQRAFLNDKMDLTQAEAIADLIDAGTAASARAALHSLQGVFSAEVNQ
LVEALIGLRIYVEAAIDFPEEEIDFLSDGKVASDLRAVRKQCLRVLGEANQGRLVREGMTLVIAGKPNAGKSSLMNALAG
FDAAIVTDIAGTTRDVLRESIQLDGMPLNLIDTAGLRDSADVVEQEGIRRAYAEMRKADRLLVMVDSRDPLDDIHNLHDL
LPQSQQQLENLDLPVTVVLNKADLSGLSTGPRADGAFVISAANGEGLDALRGHLKQVAGYQGEGQSRFSARRRHITALEK
ALMALESGEQQLHGQAAGELLAEDLRAAQKALEEITGVFTADDLLGRIFSSFCIGK

Sequences:

>Translated_456_residues
MLTSSPDTIVAIATAPGRGGVGVVRLSGPQALSIASSIVGERLLSPRLAHFCRFRNQDGDVLDEGLVISFPAPHSFTGED
VVELQGHGGPVILDMLVATCIEAGARQARAGEFSQRAFLNDKMDLTQAEAIADLIDAGTAASARAALHSLQGVFSAEVNQ
LVEALIGLRIYVEAAIDFPEEEIDFLSDGKVASDLRAVRKQCLRVLGEANQGRLVREGMTLVIAGKPNAGKSSLMNALAG
FDAAIVTDIAGTTRDVLRESIQLDGMPLNLIDTAGLRDSADVVEQEGIRRAYAEMRKADRLLVMVDSRDPLDDIHNLHDL
LPQSQQQLENLDLPVTVVLNKADLSGLSTGPRADGAFVISAANGEGLDALRGHLKQVAGYQGEGQSRFSARRRHITALEK
ALMALESGEQQLHGQAAGELLAEDLRAAQKALEEITGVFTADDLLGRIFSSFCIGK
>Mature_456_residues
MLTSSPDTIVAIATAPGRGGVGVVRLSGPQALSIASSIVGERLLSPRLAHFCRFRNQDGDVLDEGLVISFPAPHSFTGED
VVELQGHGGPVILDMLVATCIEAGARQARAGEFSQRAFLNDKMDLTQAEAIADLIDAGTAASARAALHSLQGVFSAEVNQ
LVEALIGLRIYVEAAIDFPEEEIDFLSDGKVASDLRAVRKQCLRVLGEANQGRLVREGMTLVIAGKPNAGKSSLMNALAG
FDAAIVTDIAGTTRDVLRESIQLDGMPLNLIDTAGLRDSADVVEQEGIRRAYAEMRKADRLLVMVDSRDPLDDIHNLHDL
LPQSQQQLENLDLPVTVVLNKADLSGLSTGPRADGAFVISAANGEGLDALRGHLKQVAGYQGEGQSRFSARRRHITALEK
ALMALESGEQQLHGQAAGELLAEDLRAAQKALEEITGVFTADDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=38.0851063829787, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI193082993, Length=467, Percent_Identity=37.9014989293362, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=35.6573705179283, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=59.9562363238512, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI87082120, Length=98, Percent_Identity=34.6938775510204, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=29.637526652452, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=502, Percent_Identity=30.4780876494024, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI45550813, Length=479, Percent_Identity=33.6116910229645, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_ALCBS (Q0VKU8)

Other databases:

- EMBL:   AM286690
- RefSeq:   YP_694472.1
- ProteinModelPortal:   Q0VKU8
- SMR:   Q0VKU8
- STRING:   Q0VKU8
- GeneID:   4211925
- GenomeReviews:   AM286690_GR
- KEGG:   abo:ABO_2752
- NMPDR:   fig|393595.12.peg.2762
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q0VKU8
- BioCyc:   ABOR393595:ABO_2752-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48513; Mature: 48513

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: BINDING 25-25 BINDING 83-83 BINDING 122-122 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSSPDTIVAIATAPGRGGVGVVRLSGPQALSIASSIVGERLLSPRLAHFCRFRNQDGD
CCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLDEGLVISFPAPHSFTGEDVVELQGHGGPVILDMLVATCIEAGARQARAGEFSQRAFLN
CCCCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
DKMDLTQAEAIADLIDAGTAASARAALHSLQGVFSAEVNQLVEALIGLRIYVEAAIDFPE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCCH
EEIDFLSDGKVASDLRAVRKQCLRVLGEANQGRLVREGMTLVIAGKPNAGKSSLMNALAG
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHC
FDAAIVTDIAGTTRDVLRESIQLDGMPLNLIDTAGLRDSADVVEQEGIRRAYAEMRKADR
CCHHHHHHHCCCHHHHHHHHHHCCCCCEEHEECCCCCCHHHHHHHHHHHHHHHHHHHCCE
LLVMVDSRDPLDDIHNLHDLLPQSQQQLENLDLPVTVVLNKADLSGLSTGPRADGAFVIS
EEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEE
AANGEGLDALRGHLKQVAGYQGEGQSRFSARRRHITALEKALMALESGEQQLHGQAAGEL
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LAEDLRAAQKALEEITGVFTADDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTSSPDTIVAIATAPGRGGVGVVRLSGPQALSIASSIVGERLLSPRLAHFCRFRNQDGD
CCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLDEGLVISFPAPHSFTGEDVVELQGHGGPVILDMLVATCIEAGARQARAGEFSQRAFLN
CCCCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
DKMDLTQAEAIADLIDAGTAASARAALHSLQGVFSAEVNQLVEALIGLRIYVEAAIDFPE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCCH
EEIDFLSDGKVASDLRAVRKQCLRVLGEANQGRLVREGMTLVIAGKPNAGKSSLMNALAG
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHC
FDAAIVTDIAGTTRDVLRESIQLDGMPLNLIDTAGLRDSADVVEQEGIRRAYAEMRKADR
CCHHHHHHHCCCHHHHHHHHHHCCCCCEEHEECCCCCCHHHHHHHHHHHHHHHHHHHCCE
LLVMVDSRDPLDDIHNLHDLLPQSQQQLENLDLPVTVVLNKADLSGLSTGPRADGAFVIS
EEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEE
AANGEGLDALRGHLKQVAGYQGEGQSRFSARRRHITALEKALMALESGEQQLHGQAAGEL
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LAEDLRAAQKALEEITGVFTADDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA