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The map label for this gene is pilU [H]

Identifier: 110835532

GI number: 110835532

Start: 3019041

End: 3020162

Strand: Direct

Name: pilU [H]

Synonym: ABO_2671

Alternate gene names: 110835532

Gene position: 3019041-3020162 (Clockwise)

Preceding gene: 110835531

Following gene: 110835534

Centisome position: 96.76

GC content: 56.24

Gene sequence:

>1122_bases
ATGGAATTCGAAGAGCTTCTCAAGTTGATGGTACAGAAAGGTGGGTCAGATCTGTTTATCACCGCTGGCGTACCCCCGAG
TATGAAAATCAACGGCAAGATCCTGCCGGTGACCAAAAGTGCGCTGACCCCGGATATAACACGGGACATCGTGTTTGGCG
TCATGACCGAGCAGCAGCGCAAGGAGTTCGTGGCCAACCATGAGTGTAACTTTGCCATCTCCGCCCGTGGCGTGGGCCGT
TTCCGGGTCTCTGCGTTCTATCAGCGCAACCTGGTGGGCATGGTACTGCGCCGCATCGAAACCAAAATCCCTAACGTGGA
CGACCTGCGCTTGCCGCCAGTGATAAAGGAACTGGCTATGACCAAGCGTGGCTTAGTGATTTTCGTGGGCGCTACCGGTA
CCGGTAAATCCACCTCACTGGCGTCCATGATTGGCCATCGCAACAAGAACTCCAAGGGTCACATCATCACCATTGAAGAC
CCCATCGAATTTATCCACCAGCACCAGGGCTGTATCGTCACCCAGCGTGAGGTGGGCATTGATACCGAATCCTTCGACGT
GGCCCTGAAGAACACCCTGCGCCAGGCACCGGATGTGATTCTGATTGGGGAGATCCGCACCCCTGACACCATGGACAAGG
CCATTGCTTTCGCCGAAACCGGGCACCTGTGTCTAGCTACCTTACACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATTCACTTCTTCCCGGCGGATCGTCATGGCCAGCTGTGGATGGATCTATCCCTGAACCTGAAAGGCATCGTCGCCCAGCA
ACTGATCCCCACCCCAGATGGTAAAGGCCGACGCGCGGCCATCGAAGTGCTTCTAGGCACACCGCTGGTACAAGACCATA
TCCGCAAGGGCGAAGTGCATCAGATCAAGCAGCTGATGAGCAAATCCCGGGAGCAGGGTATGCAGACTTTCGATCAAGCC
CTTTACGACCTGTACTCCGCCGGTGAAATCACCTACGAAGACGCCATGCTCCACGCCGACTCGCCCAACGACTTACGCCT
GATGATTAAGCTCGGCAACGAAACCGACGCCTCCAGTTTAGACGTGGCCACGCGGGGTCTAAGCATCGAGGATACGGAGT
AA

Upstream 100 bases:

>100_bases
TGTGGCTCGTGAGAAAGCCAAGAACCCAGATTCTCTGTAAATAGCCCCGCAACGGCCCAGCCAAGGCGCCGACAGAATCA
GTACAGACAGGCCTTAACGC

Downstream 100 bases:

>100_bases
CAGACCTTGCAGACCACGCGACTTCACTTGTATCTGCAAACAAAAAGGCCGCGCCCAAAGTATGGGCGCGGCCTTTTTCA
TTCTGGCTTCTAGAATGATG

Product: type IV pili twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MEFEELLKLMVQKGGSDLFITAGVPPSMKINGKILPVTKSALTPDITRDIVFGVMTEQQRKEFVANHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETKIPNVDDLRLPPVIKELAMTKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEIRTPDTMDKAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQLWMDLSLNLKGIVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVHQIKQLMSKSREQGMQTFDQA
LYDLYSAGEITYEDAMLHADSPNDLRLMIKLGNETDASSLDVATRGLSIEDTE

Sequences:

>Translated_373_residues
MEFEELLKLMVQKGGSDLFITAGVPPSMKINGKILPVTKSALTPDITRDIVFGVMTEQQRKEFVANHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETKIPNVDDLRLPPVIKELAMTKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEIRTPDTMDKAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQLWMDLSLNLKGIVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVHQIKQLMSKSREQGMQTFDQA
LYDLYSAGEITYEDAMLHADSPNDLRLMIKLGNETDASSLDVATRGLSIEDTE
>Mature_373_residues
MEFEELLKLMVQKGGSDLFITAGVPPSMKINGKILPVTKSALTPDITRDIVFGVMTEQQRKEFVANHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETKIPNVDDLRLPPVIKELAMTKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEIRTPDTMDKAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQLWMDLSLNLKGIVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVHQIKQLMSKSREQGMQTFDQA
LYDLYSAGEITYEDAMLHADSPNDLRLMIKLGNETDASSLDVATRGLSIEDTE

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=320, Percent_Identity=36.5625, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1789723, Length=188, Percent_Identity=33.5106382978723, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786296, Length=300, Percent_Identity=28.6666666666667, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41312; Mature: 41312

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFEELLKLMVQKGGSDLFITAGVPPSMKINGKILPVTKSALTPDITRDIVFGVMTEQQR
CCHHHHHHHHHHCCCCEEEEECCCCCCEEECCEEEEEEHHHCCCCHHHHHHHHHHHHHHH
KEFVANHECNFAISARGVGRFRVSAFYQRNLVGMVLRRIETKIPNVDDLRLPPVIKELAM
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIITIEDPIEFIHQHQGCIVTQREVGI
CCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHCCCCEEEEEHHCCC
DTESFDVALKNTLRQAPDVILIGEIRTPDTMDKAIAFAETGHLCLATLHANNANQALDRI
CCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
IHFFPADRHGQLWMDLSLNLKGIVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVH
HHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHH
QIKQLMSKSREQGMQTFDQALYDLYSAGEITYEDAMLHADSPNDLRLMIKLGNETDASSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHEEEECCCCCCEEEEEEECCCCCCCCH
DVATRGLSIEDTE
HHHHCCCCCCCCC
>Mature Secondary Structure
MEFEELLKLMVQKGGSDLFITAGVPPSMKINGKILPVTKSALTPDITRDIVFGVMTEQQR
CCHHHHHHHHHHCCCCEEEEECCCCCCEEECCEEEEEEHHHCCCCHHHHHHHHHHHHHHH
KEFVANHECNFAISARGVGRFRVSAFYQRNLVGMVLRRIETKIPNVDDLRLPPVIKELAM
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIITIEDPIEFIHQHQGCIVTQREVGI
CCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHCCCCEEEEEHHCCC
DTESFDVALKNTLRQAPDVILIGEIRTPDTMDKAIAFAETGHLCLATLHANNANQALDRI
CCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
IHFFPADRHGQLWMDLSLNLKGIVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVH
HHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHH
QIKQLMSKSREQGMQTFDQALYDLYSAGEITYEDAMLHADSPNDLRLMIKLGNETDASSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHEEEECCCCCCEEEEEEECCCCCCCCH
DVATRGLSIEDTE
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]